不同温度对樟巢螟蛹体内氧化还原状态的影响
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摘要
樟巢螟(Orthaga achatina Butler)是香樟(Cinnamomum camphora)的一种重要的害虫,其越夏蛹和越冬蛹发育经历的温度差异明显,本文研究了不同温度对樟巢螟羽化率和蛹发育过程中谷胱甘肽代谢及相关的抗氧化酶活性的影响。
1不同温度下樟巢螟羽化率的变化
25℃下,樟巢螟的羽化率是86%,而16℃和30℃时樟巢螟的羽化率较25℃的比较显著降低,分别下降为22%和76%。
2低温处理樟巢螟蛹体内抗氧化酶活性和谷胱甘肽含量变化
25℃下,GSH/GSSG从1.01降到0.87,16℃下从1.01降到0.24,标志低温条件下樟巢螟蛹内氧化胁迫严重;与25℃相比,16℃下GSH含量显著下降,GSSG含量、总谷胱甘肽含量升高,进一步说明谷胱甘肽代谢氧化加强,氧化胁迫严重;同时16℃下的樟巢螟蛹中XOD活性显著升高,表明XOD活性升高,导致H_2O_2含量积累,CAT、GST和TPX活性也显著高于25℃,更进一步证实樟巢螟蛹代谢处于过氧化状态。
3高温处理樟巢螟蛹体内抗氧化酶活性和谷胱甘肽含量变化
30℃下,GSH/GSSG从1.01降到0.50,介于25℃和16℃之间,表明高温条件下樟巢螟蛹内氧化胁迫严重,但与低温下相比氧化胁迫较轻;30℃下,XOD、CAT、GST和TPX的活性显著高于25℃,说明随着XOD活性升高,导致H_2O_2含量积累,从而提高CAT、GST和TPX的活性,谷胱甘肽氧化加强,GSH被氧化为GSSG,樟巢螟蛹处于过氧化状态。
因此,温度胁迫下,樟巢螟的羽化率显著降低,并且低温下对樟巢螟蛹的氧化胁迫较高温严重,这可能与活性氧积累,导致谷胱甘肽氧化加强有关,樟巢螟蛹处于过氧化状态。
Orthaga achatina Bulter (Lepidoptera: Pyralidae) is a significant defoliator of Cinnamomum camphora (Lauraceae). The mean temperatures during pupal development are obviously different between the overwintering and oversummering generations. Effects of different temperatures on the eclosion rate and enzymes related to glutathione metabolism were examined in Orthaga pupae.
1 Effects of different temperatures on the eclosion rate
When pupae of Orthaga achatina Bulter were transferred from 25℃to 16℃and 30℃, the eclosion rate decreased from 86% to 22% and 76%, respectively.
2 Variations of glutathione and related enzymes in the 16℃-treated pupae
Ratios of GSH and GSSG decreased from 1.01 to 0.87 in the 25℃-treated pupae and from 1.01 to 0.24 in the 16℃-treated pupae, respectively. It indicated that oxidative stress in pupae at 16℃is involved in the marked decline of eclosion rate. Compared with 25℃, the content of GSH decreased obviously,whereas the contents of GSSG and total glutathione increased. It showed that the stronger oxidation of glutathione in pupae at 16℃. Furthermore, the 16℃-treated pupae had higher activities of XOD, CAT, GST and TPX. It suggested that XOD enhancement in the 16℃-treated pupae leads to H_2O_2 accumulation, which reversely up-regulating CAT, GST and TPX.
3 Variations of glutathione and related enzymes in the 30℃-treated pupae
Ratio of GSH and GSSG decreased from 1.01 to 0.50 in the 30℃-treated pupae, which was intermediate between 25℃and 16℃. It indicated that oxidative stress in pupae at 30℃is involved in the marked decline of eclosion rate, whereas it was lower than that in pupae at 16℃. Variations of other biochemical parameters in the 30℃-treated pupae were similar to the 16℃-treated pupae.
In conclusion, the marked decline of eclosion rate of Orthaga pupae at 16℃and 30℃is involved in pro-oxidative status, and also it was more serious in 16℃-treated pupae, which may be resulted from the stronger oxidation of glutathione catalyzed by GST and TPX.
1不同温度下樟巢螟羽化率的变化
25℃下,樟巢螟的羽化率是86%,而16℃和30℃时樟巢螟的羽化率较25℃的比较显著降低,分别下降为22%和76%。
2低温处理樟巢螟蛹体内抗氧化酶活性和谷胱甘肽含量变化
25℃下,GSH/GSSG从1.01降到0.87,16℃下从1.01降到0.24,标志低温条件下樟巢螟蛹内氧化胁迫严重;与25℃相比,16℃下GSH含量显著下降,GSSG含量、总谷胱甘肽含量升高,进一步说明谷胱甘肽代谢氧化加强,氧化胁迫严重;同时16℃下的樟巢螟蛹中XOD活性显著升高,表明XOD活性升高,导致H_2O_2含量积累,CAT、GST和TPX活性也显著高于25℃,更进一步证实樟巢螟蛹代谢处于过氧化状态。
3高温处理樟巢螟蛹体内抗氧化酶活性和谷胱甘肽含量变化
30℃下,GSH/GSSG从1.01降到0.50,介于25℃和16℃之间,表明高温条件下樟巢螟蛹内氧化胁迫严重,但与低温下相比氧化胁迫较轻;30℃下,XOD、CAT、GST和TPX的活性显著高于25℃,说明随着XOD活性升高,导致H_2O_2含量积累,从而提高CAT、GST和TPX的活性,谷胱甘肽氧化加强,GSH被氧化为GSSG,樟巢螟蛹处于过氧化状态。
因此,温度胁迫下,樟巢螟的羽化率显著降低,并且低温下对樟巢螟蛹的氧化胁迫较高温严重,这可能与活性氧积累,导致谷胱甘肽氧化加强有关,樟巢螟蛹处于过氧化状态。
Orthaga achatina Bulter (Lepidoptera: Pyralidae) is a significant defoliator of Cinnamomum camphora (Lauraceae). The mean temperatures during pupal development are obviously different between the overwintering and oversummering generations. Effects of different temperatures on the eclosion rate and enzymes related to glutathione metabolism were examined in Orthaga pupae.
1 Effects of different temperatures on the eclosion rate
When pupae of Orthaga achatina Bulter were transferred from 25℃to 16℃and 30℃, the eclosion rate decreased from 86% to 22% and 76%, respectively.
2 Variations of glutathione and related enzymes in the 16℃-treated pupae
Ratios of GSH and GSSG decreased from 1.01 to 0.87 in the 25℃-treated pupae and from 1.01 to 0.24 in the 16℃-treated pupae, respectively. It indicated that oxidative stress in pupae at 16℃is involved in the marked decline of eclosion rate. Compared with 25℃, the content of GSH decreased obviously,whereas the contents of GSSG and total glutathione increased. It showed that the stronger oxidation of glutathione in pupae at 16℃. Furthermore, the 16℃-treated pupae had higher activities of XOD, CAT, GST and TPX. It suggested that XOD enhancement in the 16℃-treated pupae leads to H_2O_2 accumulation, which reversely up-regulating CAT, GST and TPX.
3 Variations of glutathione and related enzymes in the 30℃-treated pupae
Ratio of GSH and GSSG decreased from 1.01 to 0.50 in the 30℃-treated pupae, which was intermediate between 25℃and 16℃. It indicated that oxidative stress in pupae at 30℃is involved in the marked decline of eclosion rate, whereas it was lower than that in pupae at 16℃. Variations of other biochemical parameters in the 30℃-treated pupae were similar to the 16℃-treated pupae.
In conclusion, the marked decline of eclosion rate of Orthaga pupae at 16℃and 30℃is involved in pro-oxidative status, and also it was more serious in 16℃-treated pupae, which may be resulted from the stronger oxidation of glutathione catalyzed by GST and TPX.
引文
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