健胃愈疡颗粒抗消化性溃疡复发的作用及与胃黏膜疏水性的相关性研究
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摘要
目的:
本课题通过实验研究和临床研究,观察健胃愈疡颗粒(Jianweiyuyang granules,JWYY)对消化性溃疡(Peptic ulcer,PU)愈合过程中PAF、PS_2的表达以及对氨基己糖、磷脂含量和胃粘膜表面的接触角(Contact angle,CA)的影响,从胃粘膜表面疏水性的角度探讨JWYY治疗PU及及抗溃疡复发的机制。
方法:
1.实验研究:
将108只大鼠随机分为5组,即正常对照组(12只)、假手术组(12只)、模型组(36只)、JWYY组(24只),雷尼替丁组(简称Rani组)(24只)、。采用改良Okabe乙酸涂抹法复制实验性胃溃疡模型,正常对照组不造模,假手术对照组以生理盐水代替乙酸,造模后24h,JWYY组、Rani组分别灌以JWYY和雷尼替丁药液,量分别为1.62g/(kg.d)、27mg/(kg.d)体重,其余各组灌服蒸馏水,量均为1ml/100g体重,每天1次。造模第90d,再将模型组大鼠随机分为模型组和模型复发组,腹腔注射白细胞介素-1β(interleukin-1β,IL-1β)(1μg/kg体重)复制胃溃疡复发模型,模型组以生理盐水代替IL-1β。分别于造模第7d、92d(即复发溃疡诱导后48h)均分2批处死各组大鼠,剖腹取胃,沿溃疡周围切下约1×1cm~2大小的胃组织,刮取胃粘液低温冷冻保存以后作磷脂和氨基己糖测定,然后将胃组织标本一分为三:一部分将其迅速放入液氮瓶中备用,用于RT-PCR和Western blotting检测胃溃疡复发大鼠PAF、PS_2表达的变化;一部分迅速浸入4%多聚甲醛(PH7.4)中固定16h,然后常规脱水、石蜡包埋、切片、HE染色,免疫组化检测胃溃疡复发大鼠PAF、PS_2表达的变化;最后一部分作胃粘膜表面的接触角的测定,观察JWYY对氨基己糖、磷脂含量和胃粘膜表面的接触角的影响。
2.临床研究:
选取经胃镜证实的消化性溃疡(Peptic Ulcer,PU)患者(中医辨证属肝郁脾虚证者)72例,其中经胃镜和组织学及尿素酶检查证实幽门螺杆菌(Helicobacter pylori,Up)阳性的PU且中医辨证属肝郁脾虚型的患者50例JWYY治疗组和雷尼替丁(Ranitidine,Rani)对照组各36例。以上病例均符合《内科学》第5版PU的诊断标准。治疗方法:治疗组给予JWYY,每次9g口服,3次/d。对照组给予Rani,每次0.15g口服,2次/d,Hp阳性者加用阿莫西林胶囊和甲硝唑。两组均以4周为1个疗程。临床观察主要观察治疗后胃镜疗效、Hp根除率、证候疗效、1年复发率。两组PU患者均胃镜下取溃疡边缘活检标本4—5块,一块做Hp试验;一块多聚甲醛固定后石蜡切片以备免疫组织化学检查检测PAF、PS_2表达的变化;一块刮取胃黏液测氨基己糖、磷脂含量和胃粘膜表面的接触角;一至两块置液氮罐中,尔后-70℃保存以备做免疫荧光法和Western blotting用检测PAF、PS_2表达的变化。另在取得患者知情同意后,胃镜下取12个无粘膜病变患者的胃粘膜标本4—5块作为空白对照组,即正常组。正常组不作治疗和复查,只取一次标本行上述检查。一个疗程后PU患者再按照前面的方法取胃粘膜活检标本。
结果:
1.实验研究:
胃溃疡造模7d后,除正常组和假手术组,其余各组大鼠胃相应乙酸造模部位肉眼观察和组织学检查均可见到溃疡病灶,但溃疡程度不一,模型组GUI最高,JWYY组、Rani组GUI均显著低于模型组(P＜0.01)。JWYY组愈合溃疡组织形态学表现优于Rani组(P＜0.01)。
92d,JWYY组、模型复发组、Rani组复发率分别为75%、16.7%、58.3%,后二者与前者比较有显著性差异均(P＜0.01)。JWYY组比Rani组所见再生黏膜更厚,修复更完全。JWYY组比Rani组更能明显降低溃疡复发率和溃疡指数(P＜0.01)。造模92d时,JWYY组的UI和溃疡复发率明显低于其他各组(P＜0.01)。
JWYY组氨基己糖,磷脂含量和CA增高,与雷尼替丁组比差异有显著性(P＜0.01)。免疫组化及RT-PCR,Western blotting检测显示:大鼠氨基己糖,磷脂含量和CA与PAF、PS_2的表达有直线相关关系(P＜0.01),JWYY颗粒能提高PS_2和下调PAF的表达,影响胃黏膜氨基己糖及磷脂含量和CA,从而影响胃黏膜的疏水性,这可能是其促进溃疡愈合的机制之一。
2.临床研究:
我们通过临床观察发现,JWYY治疗组和Rani对照组均能有效治疗PU,两者溃疡内镜愈合率相当(P＞0.05),但治疗组较对照组更能改善患者临床症状,且1年复发率降低(P＜0.05),治疗组1年复发率为4.1%,对照组为30%,两组比较有显著性差异(P＜0.01)。而且光学显微镜观察也发现,PU患者经JWYY治疗后比治疗前,黏膜厚度增加,修复更完全溃疡床及溃疡周围炎症细胞浸润少,纤维排列较整齐,与与雷尼替丁组比差异有显著性(P＜0.01)。
JWYY组氨基己糖,磷脂含量和CA增高,与雷尼替丁组比差异有显著性(P＜0.01)。免疫组化及RT-PCR,Western blotting检测显示:PU患者氨基己糖,磷脂含量和CA和PAF、PS_2的表达有直线相关关系(P＜0.01),JWYY颗粒能提高PS_2和下调PAF的表达,影响胃黏膜氨基己糖及磷脂含量和CA,从而影响胃黏膜的疏水性,这可能是其促进溃疡愈合的机制之一。
结论:
1.从动物实验到临床观察,均证明JWYY能促进消化性溃疡愈合,并有明显的抗溃疡复发的作用。
2.JWYY颗粒通过提高PS_2和下调PAF的表达,通过影响胃黏膜氨基己糖及磷脂含量和CA,从而影响胃粘膜疏水性,加强黏液凝胶层稳定性,从而防止溃疡的产生和复发,这可能是其促进溃疡愈合的主要作用机制之一
Objective:
To investigate the effect of Jianweiyuyang(JWYY) granule on the gastric ulcer recurrence rats and the peptic ulcer patients and the expression of PAF and PS2 in the ulcer healing process and study the effect of JWYY granule on the contents of aninohexose,phosphatide and Contact angle(CA),in order to reveal the mechanisms of JWYY's effects on ulcer recurrence from the view point of hydrophobicity.
Methods:
1.Animal Experiment:
108 rats were randomly divided into five groups:control group (n=12),sham operated group(n=12),model group(n=36),Ranitidine group(n=24) and JWYY group(n=24).All rats were induced ulcer by the method of Okabe acetic squash method with some modification except for control group and sham operated group.Rats of JWYY and Ranitidine group were intragastric administrated JWYY physic liquor(1.62g/(kg.d)) and Ranitidine physic liquor(27mg/(kg.d)) respectively,one time per day,totally 90 days.The others were intragastric administrated distilled water.At 91~(st) day,rats of model group were randomly divided into model group and model recurrence group.Gastric ulcer recurrence model was duplicated by IL-1β(1μg/kg) intraperitoneal injection except for rats of model group which were intraperitoneal injection saline instead,At 7~(th),92 ~(th) day(92~(th) day is also 48h later after ulcer recurrence was induced),the rats of each group were put to death and the stomach was taken out.After get the liquid of upper gastric mucosa,then stomach wall of rats were devided into three parts.A part of stomach wall was put into the 4% formaldehydum polymerisatum for 16 hour,then it was embedded into the paraffin wax for HE staining and measure the expression of PAF and PS2 by immunohischemical.Then a part of stomach wall was put into the liquid nitrogen kettle for measure the expression of PAF and PS2 by RT-PCR and Western blotting method.The last part of stomach wall was used to measure the contact angle of gastric mucosa and the contents of aninohexose and phosphatide in ulcerated gastric mucosa for examing the hydrophobicity of gastric mucosa.
2.Clinical Research:
Choose 76 gastric ulcer patients(final diagnosis by gastroscope) were randomly divided into two groups,including JWYY treatment group,(36 cases,treatment group) and western drug control group(36 samples,control group).12 patients without gastric mucosa lesion was named normal group.Including 50 Hp-positive PU patients were confirmed by endoscopy,histological inspection,urease test,the differentiation of symptoms and signs of the traditional Chinese medicine is the stagnation of liver-QI with deficiency of the spleen type.
JWYY was used for treatment group,9g a time and 3 times a day and Ranitidine used for control group,0.15g a time and twice a day.All medicine used four weeks as a period of treatment and Amoxicillin and Metronidazole were used for the patients with lip infection additionally in Rani group.We had investigated the rate of ulcer recurrence,curative effect under gastroscope,eradicative rate of Hp,clinical curative effect in Chinese medicine syndrome.We prepared four or five pieces of tissues.They were cut from the part of gastric ulcer before treatment from two groups,one piece was used in Hp urea enzyme experiment,one pieces were fixed with 4%paraformaldehyde sotution,then wrapped up with paraffin wax and sectioned for immunohischemical,to observe the change of PAF,Ps2.one piece was used in measuring the contact angle of gastric mucosa,and the contents of aninohexose and phosphatide in ulcerated gastric mucosa for examining the hydrophobicity of gastric mucosa,another one or two pieces were stored at liquid nitrogen kettle for RT-PCR and Western blotting.To cut four or five pieces of tissues from the part of gastric organ before treatment as normal group,which were dealed with the same method and they did not treat or recheck in the whole processes.The same processes have done after a period of treatment.
Results:
1.Experiments study:
After 7 days when the recurrence model was caused,the other groups can obeserve the ulcer foci of gastric mucosa in model rats except control group and sham operated group,the ulcer foci in rats were observed macroscopically and histologically at different degree.GUI of model group is the highest,while the GUI of JWYY group and Ranitidine group were both significantly lower than that of model group(P＜0.01) Histo-morphology of both JWYY group and Ranitidine group were better than that of model group(P＜0.01).
At the 92~(th) day,ulcer relapse rate was 75%、16.7%、58.3%,in JWYY group,Model recurrence group and Ranitidine group respectively,there was significant difference between the Model recurrence group and the two treatment groups(P＜0.01).While the structure of JWYY group was relatively regulation compared with Ranitidine group The rate of the recurrence of ulcer and the ulcer index were decreased significantly in JWYY group compared with Ranitidine groups(P＜0.01).The contents of aninohexose,phosphatide and Contact angle(CA) increased in JWYY group,there was significant difference between JWYY group and Ranitidine group(P＜0.01).immuno0-hischemical, RT-PCR and Western blotting method show that There has a Linear correlation between the the contents of phosphatide, aninohexose,Contact angle(CA) and the expression of PAF,PS2 mRNA in rats(P＜0.01).JWYY granule can prevent the occurrence and relaps of ulcer and affect the hydrophobicity of gastic mucosa and strengthen the stability of myxo-gellayer by reducing the express of PAFmRNA,elevating the express of PS2mRNA and effecting the contents of phosphatide and aninohexose and Contact angle(CA),this is maybe one of the mechanisms of JWYY to heal ulcer quickly.
2.Clinical research:
Through the clinical investigation we can known that both treatment group and control group have fine effect on gastric ulcer and there are no significant difference in the rate ofventriculi curative effect(P＞0.05),but treatment group is better in ameliorate the clinical symptom than the control group,furthermore the compliance of the patients is better in JWYY group than control group(P＜0.01).The rate of the recurrence is 4.1%in treatment group and 30%in control group(P＜0.01). furthermore optical microscope investigation show that there is litter inflame- matory cell infiltration and the structure of PU patients was relatively regulation compared with other groups in JWYY group.
The contents of aninohexose,phosphatide and Contact angle(CA) of PU patients increased in JWYY group,there was significant difference between JWYY group and Ranitidine group(P＜0.01).The immuno-hischemical, RT-PCR and Western blotting method show that There has a Linear correlation between the the contents of phosphatide,aninohexose, Contact angle(CA) and the expression of PAF、PS2 mRNA in PU patients(P＜0.01).JWYY granule can prevent the occurrence and relaps of ulcer and affect the hydrophobicity of gastic mucosa and strengthen the stability of myxo-gellayer by reducing the express of PAFmRNA,elevating the express of PS2mRNA and effecting the contents of phosphatide and aninohexose and Contact angle(CA),this is maybe one of the mechanisms of JWYY to heal ulcer quickly.
Conclusions:
1.JWYY granules can accelerate the ulcer healing,ameliorate the structure of new mucosal organize and prevent the recurrence of gastric ulcer through animal experiment and clinic observation.
2.JWYY granule can prevent the occurrence and relaps of ulcer and affect the hydrophobicity of gastic mucosa and strengthen the stability of myxo-gellayer by reducing the express of PAF,elevating the express of PS2 and effecting the contents of phosphatide and aninohexose and Contact angle(CA),this is maybe one of the mecha- nisms of JWYY to heal ulcer quickly.
本课题通过实验研究和临床研究,观察健胃愈疡颗粒(Jianweiyuyang granules,JWYY)对消化性溃疡(Peptic ulcer,PU)愈合过程中PAF、PS_2的表达以及对氨基己糖、磷脂含量和胃粘膜表面的接触角(Contact angle,CA)的影响,从胃粘膜表面疏水性的角度探讨JWYY治疗PU及及抗溃疡复发的机制。
方法:
1.实验研究:
将108只大鼠随机分为5组,即正常对照组(12只)、假手术组(12只)、模型组(36只)、JWYY组(24只),雷尼替丁组(简称Rani组)(24只)、。采用改良Okabe乙酸涂抹法复制实验性胃溃疡模型,正常对照组不造模,假手术对照组以生理盐水代替乙酸,造模后24h,JWYY组、Rani组分别灌以JWYY和雷尼替丁药液,量分别为1.62g/(kg.d)、27mg/(kg.d)体重,其余各组灌服蒸馏水,量均为1ml/100g体重,每天1次。造模第90d,再将模型组大鼠随机分为模型组和模型复发组,腹腔注射白细胞介素-1β(interleukin-1β,IL-1β)(1μg/kg体重)复制胃溃疡复发模型,模型组以生理盐水代替IL-1β。分别于造模第7d、92d(即复发溃疡诱导后48h)均分2批处死各组大鼠,剖腹取胃,沿溃疡周围切下约1×1cm~2大小的胃组织,刮取胃粘液低温冷冻保存以后作磷脂和氨基己糖测定,然后将胃组织标本一分为三:一部分将其迅速放入液氮瓶中备用,用于RT-PCR和Western blotting检测胃溃疡复发大鼠PAF、PS_2表达的变化;一部分迅速浸入4%多聚甲醛(PH7.4)中固定16h,然后常规脱水、石蜡包埋、切片、HE染色,免疫组化检测胃溃疡复发大鼠PAF、PS_2表达的变化;最后一部分作胃粘膜表面的接触角的测定,观察JWYY对氨基己糖、磷脂含量和胃粘膜表面的接触角的影响。
2.临床研究:
选取经胃镜证实的消化性溃疡(Peptic Ulcer,PU)患者(中医辨证属肝郁脾虚证者)72例,其中经胃镜和组织学及尿素酶检查证实幽门螺杆菌(Helicobacter pylori,Up)阳性的PU且中医辨证属肝郁脾虚型的患者50例JWYY治疗组和雷尼替丁(Ranitidine,Rani)对照组各36例。以上病例均符合《内科学》第5版PU的诊断标准。治疗方法:治疗组给予JWYY,每次9g口服,3次/d。对照组给予Rani,每次0.15g口服,2次/d,Hp阳性者加用阿莫西林胶囊和甲硝唑。两组均以4周为1个疗程。临床观察主要观察治疗后胃镜疗效、Hp根除率、证候疗效、1年复发率。两组PU患者均胃镜下取溃疡边缘活检标本4—5块,一块做Hp试验;一块多聚甲醛固定后石蜡切片以备免疫组织化学检查检测PAF、PS_2表达的变化;一块刮取胃黏液测氨基己糖、磷脂含量和胃粘膜表面的接触角;一至两块置液氮罐中,尔后-70℃保存以备做免疫荧光法和Western blotting用检测PAF、PS_2表达的变化。另在取得患者知情同意后,胃镜下取12个无粘膜病变患者的胃粘膜标本4—5块作为空白对照组,即正常组。正常组不作治疗和复查,只取一次标本行上述检查。一个疗程后PU患者再按照前面的方法取胃粘膜活检标本。
结果:
1.实验研究:
胃溃疡造模7d后,除正常组和假手术组,其余各组大鼠胃相应乙酸造模部位肉眼观察和组织学检查均可见到溃疡病灶,但溃疡程度不一,模型组GUI最高,JWYY组、Rani组GUI均显著低于模型组(P＜0.01)。JWYY组愈合溃疡组织形态学表现优于Rani组(P＜0.01)。
92d,JWYY组、模型复发组、Rani组复发率分别为75%、16.7%、58.3%,后二者与前者比较有显著性差异均(P＜0.01)。JWYY组比Rani组所见再生黏膜更厚,修复更完全。JWYY组比Rani组更能明显降低溃疡复发率和溃疡指数(P＜0.01)。造模92d时,JWYY组的UI和溃疡复发率明显低于其他各组(P＜0.01)。
JWYY组氨基己糖,磷脂含量和CA增高,与雷尼替丁组比差异有显著性(P＜0.01)。免疫组化及RT-PCR,Western blotting检测显示:大鼠氨基己糖,磷脂含量和CA与PAF、PS_2的表达有直线相关关系(P＜0.01),JWYY颗粒能提高PS_2和下调PAF的表达,影响胃黏膜氨基己糖及磷脂含量和CA,从而影响胃黏膜的疏水性,这可能是其促进溃疡愈合的机制之一。
2.临床研究:
我们通过临床观察发现,JWYY治疗组和Rani对照组均能有效治疗PU,两者溃疡内镜愈合率相当(P＞0.05),但治疗组较对照组更能改善患者临床症状,且1年复发率降低(P＜0.05),治疗组1年复发率为4.1%,对照组为30%,两组比较有显著性差异(P＜0.01)。而且光学显微镜观察也发现,PU患者经JWYY治疗后比治疗前,黏膜厚度增加,修复更完全溃疡床及溃疡周围炎症细胞浸润少,纤维排列较整齐,与与雷尼替丁组比差异有显著性(P＜0.01)。
JWYY组氨基己糖,磷脂含量和CA增高,与雷尼替丁组比差异有显著性(P＜0.01)。免疫组化及RT-PCR,Western blotting检测显示:PU患者氨基己糖,磷脂含量和CA和PAF、PS_2的表达有直线相关关系(P＜0.01),JWYY颗粒能提高PS_2和下调PAF的表达,影响胃黏膜氨基己糖及磷脂含量和CA,从而影响胃黏膜的疏水性,这可能是其促进溃疡愈合的机制之一。
结论:
1.从动物实验到临床观察,均证明JWYY能促进消化性溃疡愈合,并有明显的抗溃疡复发的作用。
2.JWYY颗粒通过提高PS_2和下调PAF的表达,通过影响胃黏膜氨基己糖及磷脂含量和CA,从而影响胃粘膜疏水性,加强黏液凝胶层稳定性,从而防止溃疡的产生和复发,这可能是其促进溃疡愈合的主要作用机制之一
Objective:
To investigate the effect of Jianweiyuyang(JWYY) granule on the gastric ulcer recurrence rats and the peptic ulcer patients and the expression of PAF and PS2 in the ulcer healing process and study the effect of JWYY granule on the contents of aninohexose,phosphatide and Contact angle(CA),in order to reveal the mechanisms of JWYY's effects on ulcer recurrence from the view point of hydrophobicity.
Methods:
1.Animal Experiment:
108 rats were randomly divided into five groups:control group (n=12),sham operated group(n=12),model group(n=36),Ranitidine group(n=24) and JWYY group(n=24).All rats were induced ulcer by the method of Okabe acetic squash method with some modification except for control group and sham operated group.Rats of JWYY and Ranitidine group were intragastric administrated JWYY physic liquor(1.62g/(kg.d)) and Ranitidine physic liquor(27mg/(kg.d)) respectively,one time per day,totally 90 days.The others were intragastric administrated distilled water.At 91~(st) day,rats of model group were randomly divided into model group and model recurrence group.Gastric ulcer recurrence model was duplicated by IL-1β(1μg/kg) intraperitoneal injection except for rats of model group which were intraperitoneal injection saline instead,At 7~(th),92 ~(th) day(92~(th) day is also 48h later after ulcer recurrence was induced),the rats of each group were put to death and the stomach was taken out.After get the liquid of upper gastric mucosa,then stomach wall of rats were devided into three parts.A part of stomach wall was put into the 4% formaldehydum polymerisatum for 16 hour,then it was embedded into the paraffin wax for HE staining and measure the expression of PAF and PS2 by immunohischemical.Then a part of stomach wall was put into the liquid nitrogen kettle for measure the expression of PAF and PS2 by RT-PCR and Western blotting method.The last part of stomach wall was used to measure the contact angle of gastric mucosa and the contents of aninohexose and phosphatide in ulcerated gastric mucosa for examing the hydrophobicity of gastric mucosa.
2.Clinical Research:
Choose 76 gastric ulcer patients(final diagnosis by gastroscope) were randomly divided into two groups,including JWYY treatment group,(36 cases,treatment group) and western drug control group(36 samples,control group).12 patients without gastric mucosa lesion was named normal group.Including 50 Hp-positive PU patients were confirmed by endoscopy,histological inspection,urease test,the differentiation of symptoms and signs of the traditional Chinese medicine is the stagnation of liver-QI with deficiency of the spleen type.
JWYY was used for treatment group,9g a time and 3 times a day and Ranitidine used for control group,0.15g a time and twice a day.All medicine used four weeks as a period of treatment and Amoxicillin and Metronidazole were used for the patients with lip infection additionally in Rani group.We had investigated the rate of ulcer recurrence,curative effect under gastroscope,eradicative rate of Hp,clinical curative effect in Chinese medicine syndrome.We prepared four or five pieces of tissues.They were cut from the part of gastric ulcer before treatment from two groups,one piece was used in Hp urea enzyme experiment,one pieces were fixed with 4%paraformaldehyde sotution,then wrapped up with paraffin wax and sectioned for immunohischemical,to observe the change of PAF,Ps2.one piece was used in measuring the contact angle of gastric mucosa,and the contents of aninohexose and phosphatide in ulcerated gastric mucosa for examining the hydrophobicity of gastric mucosa,another one or two pieces were stored at liquid nitrogen kettle for RT-PCR and Western blotting.To cut four or five pieces of tissues from the part of gastric organ before treatment as normal group,which were dealed with the same method and they did not treat or recheck in the whole processes.The same processes have done after a period of treatment.
Results:
1.Experiments study:
After 7 days when the recurrence model was caused,the other groups can obeserve the ulcer foci of gastric mucosa in model rats except control group and sham operated group,the ulcer foci in rats were observed macroscopically and histologically at different degree.GUI of model group is the highest,while the GUI of JWYY group and Ranitidine group were both significantly lower than that of model group(P＜0.01) Histo-morphology of both JWYY group and Ranitidine group were better than that of model group(P＜0.01).
At the 92~(th) day,ulcer relapse rate was 75%、16.7%、58.3%,in JWYY group,Model recurrence group and Ranitidine group respectively,there was significant difference between the Model recurrence group and the two treatment groups(P＜0.01).While the structure of JWYY group was relatively regulation compared with Ranitidine group The rate of the recurrence of ulcer and the ulcer index were decreased significantly in JWYY group compared with Ranitidine groups(P＜0.01).The contents of aninohexose,phosphatide and Contact angle(CA) increased in JWYY group,there was significant difference between JWYY group and Ranitidine group(P＜0.01).immuno0-hischemical, RT-PCR and Western blotting method show that There has a Linear correlation between the the contents of phosphatide, aninohexose,Contact angle(CA) and the expression of PAF,PS2 mRNA in rats(P＜0.01).JWYY granule can prevent the occurrence and relaps of ulcer and affect the hydrophobicity of gastic mucosa and strengthen the stability of myxo-gellayer by reducing the express of PAFmRNA,elevating the express of PS2mRNA and effecting the contents of phosphatide and aninohexose and Contact angle(CA),this is maybe one of the mechanisms of JWYY to heal ulcer quickly.
2.Clinical research:
Through the clinical investigation we can known that both treatment group and control group have fine effect on gastric ulcer and there are no significant difference in the rate ofventriculi curative effect(P＞0.05),but treatment group is better in ameliorate the clinical symptom than the control group,furthermore the compliance of the patients is better in JWYY group than control group(P＜0.01).The rate of the recurrence is 4.1%in treatment group and 30%in control group(P＜0.01). furthermore optical microscope investigation show that there is litter inflame- matory cell infiltration and the structure of PU patients was relatively regulation compared with other groups in JWYY group.
The contents of aninohexose,phosphatide and Contact angle(CA) of PU patients increased in JWYY group,there was significant difference between JWYY group and Ranitidine group(P＜0.01).The immuno-hischemical, RT-PCR and Western blotting method show that There has a Linear correlation between the the contents of phosphatide,aninohexose, Contact angle(CA) and the expression of PAF、PS2 mRNA in PU patients(P＜0.01).JWYY granule can prevent the occurrence and relaps of ulcer and affect the hydrophobicity of gastic mucosa and strengthen the stability of myxo-gellayer by reducing the express of PAFmRNA,elevating the express of PS2mRNA and effecting the contents of phosphatide and aninohexose and Contact angle(CA),this is maybe one of the mechanisms of JWYY to heal ulcer quickly.
Conclusions:
1.JWYY granules can accelerate the ulcer healing,ameliorate the structure of new mucosal organize and prevent the recurrence of gastric ulcer through animal experiment and clinic observation.
2.JWYY granule can prevent the occurrence and relaps of ulcer and affect the hydrophobicity of gastic mucosa and strengthen the stability of myxo-gellayer by reducing the express of PAF,elevating the express of PS2 and effecting the contents of phosphatide and aninohexose and Contact angle(CA),this is maybe one of the mecha- nisms of JWYY to heal ulcer quickly.
引文
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