植物叶片延迟发光的特异性研究
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摘要
本文采用超微弱发光测量系统,以口红吊兰和绿宝石喜林芋叶片为样品,对植物叶片延迟发光的一种新特性：生物光子在生物组织中的传递行为,以及这种行为与植物叶片衰老的关系进行了研究,主要内容包括：
1.新现象的发现及其在生物光子学上的意义：在以往生物样品的延迟发光实验中,测量区域同时也是光照区域。无论是平行于叶脉还是垂直于叶脉分割叶片,同一叶片的整体与被分割的部分延迟发光规律和相互作用是相同的。在本文实验中,把叶片分为两个不同区域,一部分(A区域)被遮盖不能接受外界的光照,而另一部分(B区域)不做任何处理,光照B区域,测量A区域的延迟发光。结果发现：在外部条件一致时,整体叶片延迟发光曲线与局部叶片延迟发光出现了偏离；而且无论是只切断叶脉还是只保留叶脉(叶片其它部分保持连结),二者的延迟发光曲线均不相同,这说明叶片内部可能存在一个生物光场,光照后叶片中生物光子的传递并不依赖于叶脉或是叶肉组织等,可能存在其独特的通道；叶片的被光照区域(B区域)与被测区域(A区域)互相对换,结果表明：其延迟发光曲线衰减趋势一致,即生物光子的传递不具有方向性。
2.生物光子传递行为与叶片衰老关系的研究：对植物叶片在自然衰老过程中生物光子传递进行了测量和分析。结果表明：在自然衰老过程中,随叶龄增加,其自身发光强度先增加后减小；没有生物光子传递的叶片整体的延迟发光强度变化规律是先升高再降低,生物光子传递后叶片的延迟发光强度变化规律是先降低再升高；生物光子传递前后延迟发光衰减参数1／P的夹角先增大再减小。
3.植物叶片延迟发光的光谱特性研究：对绿宝石喜林芋成熟叶片在特定波长下的延迟发光特性进行了测量和分析,得到在400nm-640nm波长范围内其延迟发光衰减参数1／P随波长变化的光谱特性。结果表明：叶片在各个特定波长下“1／P光谱”特性不同；植物叶片延迟发光主要集中在大于500nm的长波波段,在这个波段内延迟发光强度最大；衰减参数1／P随波长的增加而上升,当波长大于500nm时,衰减参数1／P相对稳定,在这个波段条件下衰减参数1／P最大。
Using ultra-weak photon emission detective technique, we have investigated the delayed luminescence (DL) singularity which the transfer of biophoton in the biological tissue and the relation between the transfer and the senescence of plant leaves. The main contents are as follows:
1. The finding of the Novel phenomenon and the significance of the phenomenon in the biophotonics:In the former experiments of DL, the area detected is just the same area that was illuminated, the DL curve has a tendency of approximate homology between a whole leaf and their parts, whatever this leaf is divided into two parts by paralleling along leaf venation or perpendicularity to leaf venation.
In our experiments of DL, we dispose respectively two different part of a leaf, a part of the leaf (call it as A area) that is covered with opacity, other part of the leaf (B area) is illuminated, then, we measure the delayed luminescence of the A area, namely the area coverage that we measure is not the area coverage that is illuminated. The result shows that:at the similar exterior conditions, the DL curve has an angle between a whole leaf and their parts, which indicate that the DL interaction of them are dissimilarity; whatever leaf venation is cut or leaf venation is retained only(other the leaf parts are keepped in perfect). This result means that:there is a biophotons fields inside of the leaf and the biophotons fields have special passage that the transfer of biophoton independently on the nervation and the mesophyll tissue. We exchange the A area with the B area, then, B area that is covered with opacity, other A area is illuminated, we measure the delayed luminescence of the B area, the result shows that:The DL curve attenuation has a tendency of approximate homology with the state before exchange, which indicate that the transfer of biophoton haven't directivity.
2. The relation between the transfer of biophoton and the senescence of plant leaves:the transfer of biophoton was investigated during leaf natural senescence. The spontaneous luminescence intensities firstly increase and then decrease with the increasing of the leaf age in the process of natural senescence; the DL variety of the intensity of a whole leaf firstly increase and then decrease without biophotons transfer, but the DL intensity variety of the leaf firstly decrease and then increase after biophotons transfer; the DL attenuation curve without biophotons transfer have departure from the DL attenuation curve after biophotons transfer, whose change firstly increase and then decrease with the increasing of the leaf age.
3. Spectral feature of delayed luminescence of plant Leaf:Within the scope of the 400nm-640nm spectrum, we gained the change of delayed luminescence attenuation parameter (1/P) of green emerald leaves at different wave-length, which was regarded as "the 1/P spectrum". The result shows that:The characteristic of "the 1/P spectrum" of plant leaf was dissimilar at different wave-length. The intensity of the delayed luminescence ascends with the wave-length increasing. However, when the wave-length exceeds 500nm, the intensity of DL remains stable; the 1/P parameter of plant leaf also ascend with the increasing of the wave-length, but when the wave-length exceed 500nm, the 1/P parameter of remain opposite stability, whose its change is very small with the wide range of the wave-length. The rule of "the 1/P Spectrum" reflected the interior interaction of plant leaf at different excitation states.
1.新现象的发现及其在生物光子学上的意义：在以往生物样品的延迟发光实验中,测量区域同时也是光照区域。无论是平行于叶脉还是垂直于叶脉分割叶片,同一叶片的整体与被分割的部分延迟发光规律和相互作用是相同的。在本文实验中,把叶片分为两个不同区域,一部分(A区域)被遮盖不能接受外界的光照,而另一部分(B区域)不做任何处理,光照B区域,测量A区域的延迟发光。结果发现：在外部条件一致时,整体叶片延迟发光曲线与局部叶片延迟发光出现了偏离；而且无论是只切断叶脉还是只保留叶脉(叶片其它部分保持连结),二者的延迟发光曲线均不相同,这说明叶片内部可能存在一个生物光场,光照后叶片中生物光子的传递并不依赖于叶脉或是叶肉组织等,可能存在其独特的通道；叶片的被光照区域(B区域)与被测区域(A区域)互相对换,结果表明：其延迟发光曲线衰减趋势一致,即生物光子的传递不具有方向性。
2.生物光子传递行为与叶片衰老关系的研究：对植物叶片在自然衰老过程中生物光子传递进行了测量和分析。结果表明：在自然衰老过程中,随叶龄增加,其自身发光强度先增加后减小；没有生物光子传递的叶片整体的延迟发光强度变化规律是先升高再降低,生物光子传递后叶片的延迟发光强度变化规律是先降低再升高；生物光子传递前后延迟发光衰减参数1／P的夹角先增大再减小。
3.植物叶片延迟发光的光谱特性研究：对绿宝石喜林芋成熟叶片在特定波长下的延迟发光特性进行了测量和分析,得到在400nm-640nm波长范围内其延迟发光衰减参数1／P随波长变化的光谱特性。结果表明：叶片在各个特定波长下“1／P光谱”特性不同；植物叶片延迟发光主要集中在大于500nm的长波波段,在这个波段内延迟发光强度最大；衰减参数1／P随波长的增加而上升,当波长大于500nm时,衰减参数1／P相对稳定,在这个波段条件下衰减参数1／P最大。
Using ultra-weak photon emission detective technique, we have investigated the delayed luminescence (DL) singularity which the transfer of biophoton in the biological tissue and the relation between the transfer and the senescence of plant leaves. The main contents are as follows:
1. The finding of the Novel phenomenon and the significance of the phenomenon in the biophotonics:In the former experiments of DL, the area detected is just the same area that was illuminated, the DL curve has a tendency of approximate homology between a whole leaf and their parts, whatever this leaf is divided into two parts by paralleling along leaf venation or perpendicularity to leaf venation.
In our experiments of DL, we dispose respectively two different part of a leaf, a part of the leaf (call it as A area) that is covered with opacity, other part of the leaf (B area) is illuminated, then, we measure the delayed luminescence of the A area, namely the area coverage that we measure is not the area coverage that is illuminated. The result shows that:at the similar exterior conditions, the DL curve has an angle between a whole leaf and their parts, which indicate that the DL interaction of them are dissimilarity; whatever leaf venation is cut or leaf venation is retained only(other the leaf parts are keepped in perfect). This result means that:there is a biophotons fields inside of the leaf and the biophotons fields have special passage that the transfer of biophoton independently on the nervation and the mesophyll tissue. We exchange the A area with the B area, then, B area that is covered with opacity, other A area is illuminated, we measure the delayed luminescence of the B area, the result shows that:The DL curve attenuation has a tendency of approximate homology with the state before exchange, which indicate that the transfer of biophoton haven't directivity.
2. The relation between the transfer of biophoton and the senescence of plant leaves:the transfer of biophoton was investigated during leaf natural senescence. The spontaneous luminescence intensities firstly increase and then decrease with the increasing of the leaf age in the process of natural senescence; the DL variety of the intensity of a whole leaf firstly increase and then decrease without biophotons transfer, but the DL intensity variety of the leaf firstly decrease and then increase after biophotons transfer; the DL attenuation curve without biophotons transfer have departure from the DL attenuation curve after biophotons transfer, whose change firstly increase and then decrease with the increasing of the leaf age.
3. Spectral feature of delayed luminescence of plant Leaf:Within the scope of the 400nm-640nm spectrum, we gained the change of delayed luminescence attenuation parameter (1/P) of green emerald leaves at different wave-length, which was regarded as "the 1/P spectrum". The result shows that:The characteristic of "the 1/P spectrum" of plant leaf was dissimilar at different wave-length. The intensity of the delayed luminescence ascends with the wave-length increasing. However, when the wave-length exceeds 500nm, the intensity of DL remains stable; the 1/P parameter of plant leaf also ascend with the increasing of the wave-length, but when the wave-length exceed 500nm, the 1/P parameter of remain opposite stability, whose its change is very small with the wide range of the wave-length. The rule of "the 1/P Spectrum" reflected the interior interaction of plant leaf at different excitation states.
引文
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