麦粒灸对痛风性关节炎小鼠滑膜细胞功能调控研究
摘要
目的 探讨麦粒灸对痛风性关节炎小鼠滑膜细胞功能的调控及其机制。
方法 以痛风性关节炎小鼠为实验模型,分别予以麦粒灸及消炎痛治疗;以排水法进行关节肿胀程度评价;HE染色观察尿酸盐结晶及滑膜病理组织学变化;ELISA检测TNF-α、IL-2、IL-4、IL-12含量;免疫组织化学测定HSP70表达。
结果:痛风性关节炎小鼠关节肿胀、尿酸盐数目沉积增多、炎性细胞浸润、增多、关节局部TNF-α升高、IL-2、IL-12降低,艾灸后关节肿胀减轻,尿酸盐数目沉积减少,炎性细胞减少、关节局部TNF-α下降、IL-2、IL-12升高;艾灸后关节炎局部热休克蛋白(HSP 70)表达增强。
结论:麦粒灸能明显改善痛风性关节炎小鼠关节症状;其机制可能与调整关节滑膜细胞因子含量,增强关节滑膜细胞HSP70表达有关。
Objective To investigate the regulation of synovm cell function and its mechanism in gout mouse with moxibustion. Methods The gout mouse were treated respectively with indomethin and moxibustion; the swelling of joint was measured by drainage; the number of MSU and pathological feature were detected through HE colouring; The change of concentration of TNF- a ? IL-2, IL-4, IL-12 were evaluated by ELISA; the exppression of HSP70 detected by immunohistochemistry. Results: The swelling was alleviative the number of MSU was fewer after moxibustion treatment taken in gout mouse; the amount of TNF-a in joint synovm was decreased while IL-2 and IL-12 increase after 3 courses(21days) treatment with moxibustion ; the expression of HSP70 was enhanced by moxibustion. Conclusions: Wheat-moxibustion can alleviate the swelling apparently which is relation to regularting the mount of cytokine joint synovm and enhacing the expression of HSP70.
方法 以痛风性关节炎小鼠为实验模型,分别予以麦粒灸及消炎痛治疗;以排水法进行关节肿胀程度评价;HE染色观察尿酸盐结晶及滑膜病理组织学变化;ELISA检测TNF-α、IL-2、IL-4、IL-12含量;免疫组织化学测定HSP70表达。
结果:痛风性关节炎小鼠关节肿胀、尿酸盐数目沉积增多、炎性细胞浸润、增多、关节局部TNF-α升高、IL-2、IL-12降低,艾灸后关节肿胀减轻,尿酸盐数目沉积减少,炎性细胞减少、关节局部TNF-α下降、IL-2、IL-12升高;艾灸后关节炎局部热休克蛋白(HSP 70)表达增强。
结论:麦粒灸能明显改善痛风性关节炎小鼠关节症状;其机制可能与调整关节滑膜细胞因子含量,增强关节滑膜细胞HSP70表达有关。
Objective To investigate the regulation of synovm cell function and its mechanism in gout mouse with moxibustion. Methods The gout mouse were treated respectively with indomethin and moxibustion; the swelling of joint was measured by drainage; the number of MSU and pathological feature were detected through HE colouring; The change of concentration of TNF- a ? IL-2, IL-4, IL-12 were evaluated by ELISA; the exppression of HSP70 detected by immunohistochemistry. Results: The swelling was alleviative the number of MSU was fewer after moxibustion treatment taken in gout mouse; the amount of TNF-a in joint synovm was decreased while IL-2 and IL-12 increase after 3 courses(21days) treatment with moxibustion ; the expression of HSP70 was enhanced by moxibustion. Conclusions: Wheat-moxibustion can alleviate the swelling apparently which is relation to regularting the mount of cytokine joint synovm and enhacing the expression of HSP70.
引文
1 新加坡华文报刊资料7/8/1998 Pubism Page 22
2 王庆文等,原发性痛风的临床和流行痛学研究.中华内科杂志,2001,40(5):313-315
3 孟昭享.痛风.北京医科大学 中国协和医科大学联合出版社,1997,10
4 张浩宇等.痛风性关节炎的分子生物学基础.中国国伤,2001,14(3):160-162
5 郑文浩等.痛风的治疗进展。中华医学杂志 1 2 002 82(10):716-717
6 寿涛等.吲哚美辛使用情况调查及严重副作用分析,中华风湿病学杂志.2003,7(2):109-110
7 马剑颖.痛风中医治疗研究进展.江苏中医药,2002,23(6):40-41
8 唐丽.中医药外治痛风的临床进展.中国中医急症,2002,11(4):298-299
9 张淑英.针刺治疗痛风性关节炎32例.针灸临床杂志,2001:17 (08):09
10 董斌.针刺五输穴治疗痛风性关节炎25例.安徽中医学院学报,1994,(3):70
11 吴自力.针刺小肠俞对痛风镇痛效果观察.四川中医,1994:(10):54
12 李兆文.刺血疗法治疗痛风性关节炎23例.中国针灸,1993;
13 潘红玲.梅花针加拔罐治疗痛风39例.针灸临床杂志.1997;13(3):29
14 吴金钻.温针刺治疗痛风28例,针灸临床杂志,1997;13(3):41
15 黄蔚霞.药物对痛风性关节炎模型IL-1、IL-6的影响,中国中西医结合肾病杂志,2002:3(6):359
16 徐叔云主编.药理实验方法学.北京:人民卫生出版社,1985;532
17 郑文洁等.痛风的治疗进展。中华医学杂志.2002,82(10):716-717
18 陈宝兴.痛风.中华骨科杂志.2000,20(5):316-318
19 庞俊.针刺治疗痛风的体会.江苏中医1993(1):19
20 韦莉莉.取足三里、三阴交为主穴治疗急性痛风性节炎.针灸临床杂志,1997;(45):84
21 李素仁.采用针刺阳陵泉、足三里及局部取穴治疗痛风50例.四川中医,1996;14(11):53
22 陈永华.针刺治疗痛风性节炎.中国针灸,2001;21(6):377
23 周成功,针刺治疗急性痛风78例.中医外治杂志2001;10(2):5
24 孟昭亨主编.痛风.北京:北京医科大学中国协和医科大学联合出版社.1997:103-104
25 Terkeltaub R, Baird S, Sears P. et al. The murine homolog of the intedeukin-8 receptor cxcr-2 is essential for the occurrence of neutrophilic inflammation in air pouch model of acute urate crystal-induced gouty synovitis. Arthritis Rheum, 1998, 41 (5): 900-909
26 Terkeltaub R. Pathogenesis and treatment of crystal-induced inflammation In: Koopman WJ. Arthritis and allied conditions: a text book rheematology. Baltimore: Williams and Wilkins, 1996, 2085-2102
27 Terkeltaub R.Pathogenesis and treatment of crystal-induced inflammation In: Mccarty DJ, Koopman WJ.Arthritis and allied conditions: Philadelphia: 1993, 1819-1833
28 Weinberger A. Gout, uric acid metabolism and crystal-induced inflammation. Curr Pin Rheumatol, 1995,7:359-363
29 Hansbroughn JF, Wikstrom ,Braide M, et al. Neurophil activation and tissue neutrophil sequestration in a rat model of themal injury.J SurgRes.l996,61(l):17-22
30 Chosay JG, Essan NA, Dunn CJ, et al. Neutrophil margination and extravasation in sinusoids and venules of licer during endotoxin-induced injury. Am J Physiol, 1997,272(1): 1195-1200
31 Berends C. Inhibition of PAF-induced expression of CD11b and shedding of L-selection on human neutrophils and eosinophils by the type 1 selective PDE inhibitor, roliram. J Eur Respir, 1997, 10 (50): 1000-1007
32 Miguelez R, Palacios I, Navarro F, et al. Anti-inflammatory effect of a PAF receptory antagonist and a new molecule with antiproctimmn ativity in an experimental model of acute urate crystal arthritis lipid. Mediat Cell Sigal, 1996,13:35-49
33 Fujiwara Y, Arakawa T, Fukuda T, et al. Interleukin-8 stimulates leukocyte migration across a monolayer of cultured rabbit TNF- α , IL-1 β ,IL-8, and IL-1ra in Monosodium Urate Crystal induced rabbit arthritis. Lab Invest,1998,78(5):559-569
34 Akihiro Mastsukawa,Teizo Yoshimura Kazuhiko Miyamoto, et al. Analysis of the Inflammatory Cytokine Network among TNF- α , IL-1 β ,IL-1 receptor antagonist, and IL-8 in LPS-Induced Rabbit Arthritis. Laboratory Investigation. 1997;76(50:629
35 Xing Z, Kirpalaut H, Torry D, et al. polymorphonuclear leukocytes as a significant source of tumor necrosis factor-alpha in endotoxin challenged lung tissue. Am J Pathol,1993,143(4):1009-1016
36 Baggioline M, Dewald B, mosev B. IL-8 and related chemotactic cytokines CXC and Chemokines. Adv Immunol, 1994,55:97-179
37 Nishimura A, Akahoshi T, Takahashi M, et al. Attenuation of monosodium urate crystal induced arthritis in rabbits by neuralizing antibody against IL-8. J Leukoc Biol, 1997,62:441-449
38 Matsukawa A, Yoshimura T, Maeda T, et al. Neutrophil accumalation and activiation by homologons IL-8 in rabbits: IL-8 induced destruction of cartiologe and produced of IL-1 and IL-1 Ra in vivo. J Immunol, 1995,154:5419-5424
39 Di Giovine FS, Malawista SE, Nuki G, et al, Intereukin-1(IL-1) as a mediator of crystal arthritis: Stimulation of T cell and synovial fibroblast mitogenesis by urate crystal-induced IL-1. J Immunol, 1987, 138: 3213-3218
40 Udhya T, et al. Corticotropin-releasing factor suppresses intedeukin-2 induced lymphocyte proliferation and increases cAMP production in human splenocytes. Clin Res, 1991; 38: 461
41 王锁英.白细胞介素12与感染性疾病.国外医学儿科学分册,1998;25 (5):241
42 杨家和,钱其军.白细胞介素12:重要的免疫调节因子.国外医学临床生物化学与检验学分册.1999;20(3):118
43 Nelms K, Keegan AD, Zamorano J, et al. Annu Rev Immunol, 1999,17:701-738
44 Hage T, Sebald W, Reinemer P. Cell, 1999, 97(2): 271-281
45 Pan PY, Rothman P. Curr Opin in Immun,1999,11(6):615-620
46 Whitehead JP, Clark SF, James DE, et al. Curr Opinion in Cell Bio, 2000, 12(20: 222-228
47 Villar J, et al. Induction of heat stress protein is associated with decreased mortality in an animal model of acute lung injury. Am Rev Respir Dis, 1993; 147: 177-81
48 Maber MS, et al. Cardiac stress protein elevation 24-hours after brief ischemia or heat stress is associated with resistance to myocardial infarction. Circulation, 1993; 88: 1264
49 黄炳,等.健脾益气方对大鼠急性胃粘膜损伤预防作用的实验研究.同济医科大学学报,1997;26(6):453-6
50 路敦跃,等.脑缺血再灌流大鼠海马热休克蛋白70基因表达与细胞凋亡.中华医学杂志,1997;77:451-
51 马玉红,等.热休克处理对巨噬细胞HSP70、HLA-DR表达的影响.中国实验临床免疫学杂志,1999;11(5):56-8
52 Hirvonen MR, et al. Heat shock proteins and macrophages resistance to the toxic effects of nitric oxide. Biochem J, 1996, 315(pt3): 845
53 Kobayashi K, et al. Relationship between moxibustion and heat-shock proteins. J Clin & Exp Med. 1989; 148: 199-200
54 Kobayashi K. Induction of heat-shock protein (hsp) by Moxibustion.
2 王庆文等,原发性痛风的临床和流行痛学研究.中华内科杂志,2001,40(5):313-315
3 孟昭享.痛风.北京医科大学 中国协和医科大学联合出版社,1997,10
4 张浩宇等.痛风性关节炎的分子生物学基础.中国国伤,2001,14(3):160-162
5 郑文浩等.痛风的治疗进展。中华医学杂志 1 2 002 82(10):716-717
6 寿涛等.吲哚美辛使用情况调查及严重副作用分析,中华风湿病学杂志.2003,7(2):109-110
7 马剑颖.痛风中医治疗研究进展.江苏中医药,2002,23(6):40-41
8 唐丽.中医药外治痛风的临床进展.中国中医急症,2002,11(4):298-299
9 张淑英.针刺治疗痛风性关节炎32例.针灸临床杂志,2001:17 (08):09
10 董斌.针刺五输穴治疗痛风性关节炎25例.安徽中医学院学报,1994,(3):70
11 吴自力.针刺小肠俞对痛风镇痛效果观察.四川中医,1994:(10):54
12 李兆文.刺血疗法治疗痛风性关节炎23例.中国针灸,1993;
13 潘红玲.梅花针加拔罐治疗痛风39例.针灸临床杂志.1997;13(3):29
14 吴金钻.温针刺治疗痛风28例,针灸临床杂志,1997;13(3):41
15 黄蔚霞.药物对痛风性关节炎模型IL-1、IL-6的影响,中国中西医结合肾病杂志,2002:3(6):359
16 徐叔云主编.药理实验方法学.北京:人民卫生出版社,1985;532
17 郑文洁等.痛风的治疗进展。中华医学杂志.2002,82(10):716-717
18 陈宝兴.痛风.中华骨科杂志.2000,20(5):316-318
19 庞俊.针刺治疗痛风的体会.江苏中医1993(1):19
20 韦莉莉.取足三里、三阴交为主穴治疗急性痛风性节炎.针灸临床杂志,1997;(45):84
21 李素仁.采用针刺阳陵泉、足三里及局部取穴治疗痛风50例.四川中医,1996;14(11):53
22 陈永华.针刺治疗痛风性节炎.中国针灸,2001;21(6):377
23 周成功,针刺治疗急性痛风78例.中医外治杂志2001;10(2):5
24 孟昭亨主编.痛风.北京:北京医科大学中国协和医科大学联合出版社.1997:103-104
25 Terkeltaub R, Baird S, Sears P. et al. The murine homolog of the intedeukin-8 receptor cxcr-2 is essential for the occurrence of neutrophilic inflammation in air pouch model of acute urate crystal-induced gouty synovitis. Arthritis Rheum, 1998, 41 (5): 900-909
26 Terkeltaub R. Pathogenesis and treatment of crystal-induced inflammation In: Koopman WJ. Arthritis and allied conditions: a text book rheematology. Baltimore: Williams and Wilkins, 1996, 2085-2102
27 Terkeltaub R.Pathogenesis and treatment of crystal-induced inflammation In: Mccarty DJ, Koopman WJ.Arthritis and allied conditions: Philadelphia: 1993, 1819-1833
28 Weinberger A. Gout, uric acid metabolism and crystal-induced inflammation. Curr Pin Rheumatol, 1995,7:359-363
29 Hansbroughn JF, Wikstrom ,Braide M, et al. Neurophil activation and tissue neutrophil sequestration in a rat model of themal injury.J SurgRes.l996,61(l):17-22
30 Chosay JG, Essan NA, Dunn CJ, et al. Neutrophil margination and extravasation in sinusoids and venules of licer during endotoxin-induced injury. Am J Physiol, 1997,272(1): 1195-1200
31 Berends C. Inhibition of PAF-induced expression of CD11b and shedding of L-selection on human neutrophils and eosinophils by the type 1 selective PDE inhibitor, roliram. J Eur Respir, 1997, 10 (50): 1000-1007
32 Miguelez R, Palacios I, Navarro F, et al. Anti-inflammatory effect of a PAF receptory antagonist and a new molecule with antiproctimmn ativity in an experimental model of acute urate crystal arthritis lipid. Mediat Cell Sigal, 1996,13:35-49
33 Fujiwara Y, Arakawa T, Fukuda T, et al. Interleukin-8 stimulates leukocyte migration across a monolayer of cultured rabbit TNF- α , IL-1 β ,IL-8, and IL-1ra in Monosodium Urate Crystal induced rabbit arthritis. Lab Invest,1998,78(5):559-569
34 Akihiro Mastsukawa,Teizo Yoshimura Kazuhiko Miyamoto, et al. Analysis of the Inflammatory Cytokine Network among TNF- α , IL-1 β ,IL-1 receptor antagonist, and IL-8 in LPS-Induced Rabbit Arthritis. Laboratory Investigation. 1997;76(50:629
35 Xing Z, Kirpalaut H, Torry D, et al. polymorphonuclear leukocytes as a significant source of tumor necrosis factor-alpha in endotoxin challenged lung tissue. Am J Pathol,1993,143(4):1009-1016
36 Baggioline M, Dewald B, mosev B. IL-8 and related chemotactic cytokines CXC and Chemokines. Adv Immunol, 1994,55:97-179
37 Nishimura A, Akahoshi T, Takahashi M, et al. Attenuation of monosodium urate crystal induced arthritis in rabbits by neuralizing antibody against IL-8. J Leukoc Biol, 1997,62:441-449
38 Matsukawa A, Yoshimura T, Maeda T, et al. Neutrophil accumalation and activiation by homologons IL-8 in rabbits: IL-8 induced destruction of cartiologe and produced of IL-1 and IL-1 Ra in vivo. J Immunol, 1995,154:5419-5424
39 Di Giovine FS, Malawista SE, Nuki G, et al, Intereukin-1(IL-1) as a mediator of crystal arthritis: Stimulation of T cell and synovial fibroblast mitogenesis by urate crystal-induced IL-1. J Immunol, 1987, 138: 3213-3218
40 Udhya T, et al. Corticotropin-releasing factor suppresses intedeukin-2 induced lymphocyte proliferation and increases cAMP production in human splenocytes. Clin Res, 1991; 38: 461
41 王锁英.白细胞介素12与感染性疾病.国外医学儿科学分册,1998;25 (5):241
42 杨家和,钱其军.白细胞介素12:重要的免疫调节因子.国外医学临床生物化学与检验学分册.1999;20(3):118
43 Nelms K, Keegan AD, Zamorano J, et al. Annu Rev Immunol, 1999,17:701-738
44 Hage T, Sebald W, Reinemer P. Cell, 1999, 97(2): 271-281
45 Pan PY, Rothman P. Curr Opin in Immun,1999,11(6):615-620
46 Whitehead JP, Clark SF, James DE, et al. Curr Opinion in Cell Bio, 2000, 12(20: 222-228
47 Villar J, et al. Induction of heat stress protein is associated with decreased mortality in an animal model of acute lung injury. Am Rev Respir Dis, 1993; 147: 177-81
48 Maber MS, et al. Cardiac stress protein elevation 24-hours after brief ischemia or heat stress is associated with resistance to myocardial infarction. Circulation, 1993; 88: 1264
49 黄炳,等.健脾益气方对大鼠急性胃粘膜损伤预防作用的实验研究.同济医科大学学报,1997;26(6):453-6
50 路敦跃,等.脑缺血再灌流大鼠海马热休克蛋白70基因表达与细胞凋亡.中华医学杂志,1997;77:451-
51 马玉红,等.热休克处理对巨噬细胞HSP70、HLA-DR表达的影响.中国实验临床免疫学杂志,1999;11(5):56-8
52 Hirvonen MR, et al. Heat shock proteins and macrophages resistance to the toxic effects of nitric oxide. Biochem J, 1996, 315(pt3): 845
53 Kobayashi K, et al. Relationship between moxibustion and heat-shock proteins. J Clin & Exp Med. 1989; 148: 199-200
54 Kobayashi K. Induction of heat-shock protein (hsp) by Moxibustion.