抗变链鸡卵黄抗体（IgY）对两种变形链球菌粘附的影响
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摘要
目的 探讨抗变形链球菌和茸毛链球菌鸡卵黄抗体IgY抑制这
两种变链菌对唾液包被羟基磷灰生（S-HA）的粘附作用及最佳浓
度。
方法 将用S.mutans MT6R（遗传I型，血清C型）和S.sobrinus
6715（遗传Ⅲ型，血清g型）加弗氏佐剂免疫母鸡后，从其产下鸡
蛋黄中提取的特异性IgY抗体作梯度稀释，分别观察不同稀释倍数
的特异性IgY抗体对S.mutans MT6R，S.sobrinus 6715和S.mutans
MT6R及S.sobrinus 6715混合组粘附的影响。
结果 发现原倍和稀释倍数为1:2，1:4，1:8，1:16的
IgY抗体分别能明显抑制S.mutans MT6R，S.sobrinus 6715和S.
mutans MT6R及S.sobrinus 6715对S-HA的粘附（p＜0.05），当稀释倍
数达32倍时，IgY抗体对S.mutans MT6R和S.sobrinus的粘附无明显
抑制效应（p＞0.05）。本实验显示，当稀释倍数为1:2时，变链菌
的粘附率最低。
结论 抗S.mutans MT6R和S.sobrinus 6715的IgY抗体能干扰这
两种变链菌的粘附，1:2稀释倍数浓度的IgY抗体抗S.mutans
MT6R和S.sobrinus 6715粘附到S-HA能力最佳。
Objective To investigate the effects of polyclonal hen-egg-yolk
antibodies(IgY)to S.mutans and S.sobrinus on streptococcal adherence
arid its best adherence-inhibiting concentration. Methods applying
the specific JgY antibodies purified from the egg-yolk laid by the hen
imm i tnized with S .mutans MT6R (serotype c)S .sobrinus 6715 (serotype
g) attd Freud adjuvant,and marked S.mutans MT6R and S.sobrinus
6715 with to investigate the IgY antibodies of different
concentration on these two streptococcal~dherence to saliva-coated HA
by diluting the antibodies in the way of 1:1,1:2,1:4,1:8,1:16,1:32 in
vitro. Results The results showed that the IgY antibodies with a
concentration of 1:1,1:2,1:4,1:8,1:1 6,could significantly inhibit S.
rnutans,S.sobrinus and S.mutans+S.sobrinus to adhere to S-HA
resp cctively (p is I. :2 of original concenflation. Conclusion The IgY antibodies could
inhibit streptococcal adherence significantly and the best adherence-
inhibiting concentration is 1:2 of the original.
两种变链菌对唾液包被羟基磷灰生（S-HA）的粘附作用及最佳浓
度。
方法 将用S.mutans MT6R（遗传I型，血清C型）和S.sobrinus
6715（遗传Ⅲ型，血清g型）加弗氏佐剂免疫母鸡后，从其产下鸡
蛋黄中提取的特异性IgY抗体作梯度稀释，分别观察不同稀释倍数
的特异性IgY抗体对S.mutans MT6R，S.sobrinus 6715和S.mutans
MT6R及S.sobrinus 6715混合组粘附的影响。
结果 发现原倍和稀释倍数为1:2，1:4，1:8，1:16的
IgY抗体分别能明显抑制S.mutans MT6R，S.sobrinus 6715和S.
mutans MT6R及S.sobrinus 6715对S-HA的粘附（p＜0.05），当稀释倍
数达32倍时，IgY抗体对S.mutans MT6R和S.sobrinus的粘附无明显
抑制效应（p＞0.05）。本实验显示，当稀释倍数为1:2时，变链菌
的粘附率最低。
结论 抗S.mutans MT6R和S.sobrinus 6715的IgY抗体能干扰这
两种变链菌的粘附，1:2稀释倍数浓度的IgY抗体抗S.mutans
MT6R和S.sobrinus 6715粘附到S-HA能力最佳。
Objective To investigate the effects of polyclonal hen-egg-yolk
antibodies(IgY)to S.mutans and S.sobrinus on streptococcal adherence
arid its best adherence-inhibiting concentration. Methods applying
the specific JgY antibodies purified from the egg-yolk laid by the hen
imm i tnized with S .mutans MT6R (serotype c)S .sobrinus 6715 (serotype
g) attd Freud adjuvant,and marked S.mutans MT6R and S.sobrinus
6715 with to investigate the IgY antibodies of different
concentration on these two streptococcal~dherence to saliva-coated HA
by diluting the antibodies in the way of 1:1,1:2,1:4,1:8,1:16,1:32 in
vitro. Results The results showed that the IgY antibodies with a
concentration of 1:1,1:2,1:4,1:8,1:1 6,could significantly inhibit S.
rnutans,S.sobrinus and S.mutans+S.sobrinus to adhere to S-HA
resp cctively (p
inhibit streptococcal adherence significantly and the best adherence-
inhibiting concentration is 1:2 of the original.
引文
1.Hamada s.T.Koga, and T.Ooshima Virulence factors of streptococcus mutans and dental caries prevention.J.Dent,Res. 1984,63:407-411
2．张平，樊明文，边专等，单克隆抗体对茸毛链球菌粘附及龋病发生的影响，中华口腔医学杂志，1999，34(2)：109
3。Brady LJ,et al.Differentiation of Salivary agglutinin mediated adherence and aggre gation of mutans streptococci by use of monnoclonal antibodies against the major surface adhesin pl.Infect Immun, 1992,60:1008
4.Hajishengallis 9;T Koga,M W Russell. Affinity and specificity of the interaction between S.mutans antigen I/II and salivary components J Dent Res, 1992,91:12
5.Wennerholm K.D Birk hed, C G Emilson,Effects of sugar restriction on S.mutans and S.sobrinus in Saliva and dental plaque.Caries Res, 1995,29.
6.Nesbitt. WE,Been,E,et al. Adherence inhibition of actinomyces viscosus by a monoclonal antibody to type 1 fimbriae associated adhesive proteins.Dent Res, 1991,abstr 417,317
7.Crawford PC,Clark WB.Fimbria-specific antibodies in serum and saliva of mice immunized with actinomyces viscosus T14v firnbria Infect Immun, 1986,54:507
8.Otake. S.et al,Protection of rats against dental caries by passive immunization with hen-egg-yolk antibody(IgY),1991,J.Dent,Res,70:162-166
9.Hamada, S,et al,Oral passive immunization against dental caries in Rats by use of hen-egg-yolk antibodies specific for cell-associated Glucosyltransferase of streptoccus mutans, 1991,Infect Immun,59(11):4161-4167
10.Hatta H, et al,Passive immunization against dental plaque roemation in humans:Effect of a mouth rinse containing egg-yolk antibody(IgY).Caries Res. 1997,31 (4):268-274
11．顾淑萍，刘正，宋培智，乳杆菌和变链菌的牙本质能力实验。习体牙髓牙周病学杂志，1999，9(1)：8-10
12．黄定明，罗宗莲，变形链球菌和远缘链球菌致龋性的研究近况，上海口腔医学，1996，5(2)：98-101
13.Lehner T,et al. Local passive immunization by monoclonal antibodies against streptococcus antigen I/II in the prevention of dental caries. Infect Immun, 1985;50:796
14．文冰等，抗变形链球菌IgY抗体抑制变链菌产酸及合成葡聚糖的实验研究，待发表。
15、樊明文，张平，边专，免疫防龋的研究新进展，中华口腔医学杂志，1999，34(2)：69-72。
2．张平，樊明文，边专等，单克隆抗体对茸毛链球菌粘附及龋病发生的影响，中华口腔医学杂志，1999，34(2)：109
3。Brady LJ,et al.Differentiation of Salivary agglutinin mediated adherence and aggre gation of mutans streptococci by use of monnoclonal antibodies against the major surface adhesin pl.Infect Immun, 1992,60:1008
4.Hajishengallis 9;T Koga,M W Russell. Affinity and specificity of the interaction between S.mutans antigen I/II and salivary components J Dent Res, 1992,91:12
5.Wennerholm K.D Birk hed, C G Emilson,Effects of sugar restriction on S.mutans and S.sobrinus in Saliva and dental plaque.Caries Res, 1995,29.
6.Nesbitt. WE,Been,E,et al. Adherence inhibition of actinomyces viscosus by a monoclonal antibody to type 1 fimbriae associated adhesive proteins.Dent Res, 1991,abstr 417,317
7.Crawford PC,Clark WB.Fimbria-specific antibodies in serum and saliva of mice immunized with actinomyces viscosus T14v firnbria Infect Immun, 1986,54:507
8.Otake. S.et al,Protection of rats against dental caries by passive immunization with hen-egg-yolk antibody(IgY),1991,J.Dent,Res,70:162-166
9.Hamada, S,et al,Oral passive immunization against dental caries in Rats by use of hen-egg-yolk antibodies specific for cell-associated Glucosyltransferase of streptoccus mutans, 1991,Infect Immun,59(11):4161-4167
10.Hatta H, et al,Passive immunization against dental plaque roemation in humans:Effect of a mouth rinse containing egg-yolk antibody(IgY).Caries Res. 1997,31 (4):268-274
11．顾淑萍，刘正，宋培智，乳杆菌和变链菌的牙本质能力实验。习体牙髓牙周病学杂志，1999，9(1)：8-10
12．黄定明，罗宗莲，变形链球菌和远缘链球菌致龋性的研究近况，上海口腔医学，1996，5(2)：98-101
13.Lehner T,et al. Local passive immunization by monoclonal antibodies against streptococcus antigen I/II in the prevention of dental caries. Infect Immun, 1985;50:796
14．文冰等，抗变形链球菌IgY抗体抑制变链菌产酸及合成葡聚糖的实验研究，待发表。
15、樊明文，张平，边专，免疫防龋的研究新进展，中华口腔医学杂志，1999，34(2)：69-72。