BTH诱导葫芦科作物抗病毒研究
摘要
小西葫芦黄花叶病毒(Zuccini yellow mosaic virus, ZYMV)、西瓜花叶病毒(Watermelon mosaic virus, WMV)、黄瓜花叶病毒(Cucumber mosaic virus, CMV)是葫芦科作物上普遍发生、危害严重的3种病毒。本研究以这3种病毒为研究对象,考察苯并噻二唑类化合物S-甲基苯基(1,2,3)噻二唑-7-硫代羧酸酯(benzothiodiazole, BTH)处理后,植株对ZYMV、WMV、CMV的抗病效果。在此基础上,采用以18SrRNA为内参的半定量RT-PCR方法,对BTH诱导西葫芦抗ZYMV、WMV、CMV的作用机理进行了探讨。获得的主要研究结果如下:
1.BTH处理后,植株生长量减少;BTH诱导不同葫芦科作物抗病毒病所需的适宜浓度不同。BTH诱导西瓜、甜瓜抗ZYMV、WMV、CMV所需的适宜浓度为25μg/ml;BTH诱导西葫芦抗ZYMV、WMV、CMV所需的适宜浓度为50μg/ml。
2.在接种ZYMV、WMV前3 d用25μg/mlBTH处理西瓜,28 d后植株的病情指数减少,相对防效分别为55.6%、56.8%;在接种CMV前3 d用25μg/ml BTH处理甜瓜,28 d后植株的病情指数减少,相对防效为78.2%。进一步研究发现:BTH处理后,处理与接种的间隔期即诱导时间的长短对植株接种后的病情指数有一定的影响;BTH对病毒病的防治效果优于病毒A、病毒K、宁南霉素、芸薹素内酯。
3.在接种ZYMV、WMV、CMV前3 d用50μg/ml BTH处理西葫芦,28 d后植株的病情指数减少,相对防效分别为39.3%、45.4%、87.5%;在接种ZYMV、WMV、CMV后1d和8d分别用50μg/ml BTH处理西葫芦,第一次处理28 d后植株的病情指数减少,相对防效分别为40.6%、38.7%、54.8%;在接种ZYMV、WMV、CMV前3 d用50μg/mlBTH处理西葫芦,接种后3 d定植到大田,定植后搭防虫网,28 d后植株的病情指数减少,相对防效分别为38.2%、42.0%、54.4%。
4.采用以18SrRNA为内参的半定量RT-PCR方法,对分别接种ZYMV、WMV、CMV(接种前3 d 50μg/ml BTH处理植株)后第28 d时新生叶片病毒繁殖量进行分析,结果表明:BTH处理能抑制植株叶片内WMV、CMV复制,不能抑制植株叶片内ZYMV的复制。
Zuccini yellow mosaic virus(ZYMV),Watermelon mosaic virus(WMV)and Cucumber mosaic virus(CMV)are the most important pathogens infecting cucurbitaceous crops. The potential effect of benzothiodiazole to trigger systemic acquired resistance (SAR) in plants against ZYMV, WMV, CMV was investigated by treatment of plants with BTH;With the aid of Semi-quantityitive RT-PCR with the plant’s 18SrRNA as internal control, we studied the resistance mechanism too. The results obtained in this study were illustrated as followings:
1. Average fresh weigh decreased after treatment of plants with BTH; The better concentration of pre-treatment with BTH to watermelon, melon is 25μg/ml; the better concentration of pre-treatment with BTH to zucchini is 50μg/ml .
2. The resistance was evident as a reduced disease severity in BTH-treated plants. Average relative control effect 28 days after treatments reached 55.6%, 56.8% for ZYMV, WMV respectively when pre-treatment of watermelon with 25μg/ml BTH 3 days before virus inoculations; Average relative control effect 28 days after treatments reached 78.2% for CMV when pre-treatment of melon with 25μg/ml BTH 3 days before virus inoculations. The further research indicated that the time between treatment of BTH and the inoculation also affected disease indices; The potential effect of BTH is better than that of Moroxydine hydrochloride copper acetate, K virusicide, Ningnanmycin, Brassinolide.
3. Average relative control effect 28 days after treatments reached 39.3%, 45.4%, 87.5% for ZYMV, WMV, CMV respectively when pre-treatment of zucchini with 50μg/ml BTH 3 days before virus inoculations; Average relative control effect 28 days after first treatments also reached 40.6%, 38.7%, 54.8% for ZYMV, WMV, CMV respectively when BTH applied ,1day and 8 days after virus inoculation;Average relative control effect 28 days after treatments reached 38.2%, 42.0%, 54.4% for ZYMV, WMV, CMV respectively when pre-treatment of zucchini with 50μg/ml BTH 3 days before virus inoculations, outdoor cultivation with insect screen 3 days after inoculation.
4. The virus concentration of newly developed leaves of plants 28 days after 50μg/ml BTH treatments 3 days before inoculation was analyzed by semi-quantitive RT-PCR with the plant’s 18S rRNA as internal control. The results were indicated that 50μg/ml BTH treatment 3 days before inoculation affected WMV, CMV replication but did not affect ZYMV replication.
1.BTH处理后,植株生长量减少;BTH诱导不同葫芦科作物抗病毒病所需的适宜浓度不同。BTH诱导西瓜、甜瓜抗ZYMV、WMV、CMV所需的适宜浓度为25μg/ml;BTH诱导西葫芦抗ZYMV、WMV、CMV所需的适宜浓度为50μg/ml。
2.在接种ZYMV、WMV前3 d用25μg/mlBTH处理西瓜,28 d后植株的病情指数减少,相对防效分别为55.6%、56.8%;在接种CMV前3 d用25μg/ml BTH处理甜瓜,28 d后植株的病情指数减少,相对防效为78.2%。进一步研究发现:BTH处理后,处理与接种的间隔期即诱导时间的长短对植株接种后的病情指数有一定的影响;BTH对病毒病的防治效果优于病毒A、病毒K、宁南霉素、芸薹素内酯。
3.在接种ZYMV、WMV、CMV前3 d用50μg/ml BTH处理西葫芦,28 d后植株的病情指数减少,相对防效分别为39.3%、45.4%、87.5%;在接种ZYMV、WMV、CMV后1d和8d分别用50μg/ml BTH处理西葫芦,第一次处理28 d后植株的病情指数减少,相对防效分别为40.6%、38.7%、54.8%;在接种ZYMV、WMV、CMV前3 d用50μg/mlBTH处理西葫芦,接种后3 d定植到大田,定植后搭防虫网,28 d后植株的病情指数减少,相对防效分别为38.2%、42.0%、54.4%。
4.采用以18SrRNA为内参的半定量RT-PCR方法,对分别接种ZYMV、WMV、CMV(接种前3 d 50μg/ml BTH处理植株)后第28 d时新生叶片病毒繁殖量进行分析,结果表明:BTH处理能抑制植株叶片内WMV、CMV复制,不能抑制植株叶片内ZYMV的复制。
Zuccini yellow mosaic virus(ZYMV),Watermelon mosaic virus(WMV)and Cucumber mosaic virus(CMV)are the most important pathogens infecting cucurbitaceous crops. The potential effect of benzothiodiazole to trigger systemic acquired resistance (SAR) in plants against ZYMV, WMV, CMV was investigated by treatment of plants with BTH;With the aid of Semi-quantityitive RT-PCR with the plant’s 18SrRNA as internal control, we studied the resistance mechanism too. The results obtained in this study were illustrated as followings:
1. Average fresh weigh decreased after treatment of plants with BTH; The better concentration of pre-treatment with BTH to watermelon, melon is 25μg/ml; the better concentration of pre-treatment with BTH to zucchini is 50μg/ml .
2. The resistance was evident as a reduced disease severity in BTH-treated plants. Average relative control effect 28 days after treatments reached 55.6%, 56.8% for ZYMV, WMV respectively when pre-treatment of watermelon with 25μg/ml BTH 3 days before virus inoculations; Average relative control effect 28 days after treatments reached 78.2% for CMV when pre-treatment of melon with 25μg/ml BTH 3 days before virus inoculations. The further research indicated that the time between treatment of BTH and the inoculation also affected disease indices; The potential effect of BTH is better than that of Moroxydine hydrochloride copper acetate, K virusicide, Ningnanmycin, Brassinolide.
3. Average relative control effect 28 days after treatments reached 39.3%, 45.4%, 87.5% for ZYMV, WMV, CMV respectively when pre-treatment of zucchini with 50μg/ml BTH 3 days before virus inoculations; Average relative control effect 28 days after first treatments also reached 40.6%, 38.7%, 54.8% for ZYMV, WMV, CMV respectively when BTH applied ,1day and 8 days after virus inoculation;Average relative control effect 28 days after treatments reached 38.2%, 42.0%, 54.4% for ZYMV, WMV, CMV respectively when pre-treatment of zucchini with 50μg/ml BTH 3 days before virus inoculations, outdoor cultivation with insect screen 3 days after inoculation.
4. The virus concentration of newly developed leaves of plants 28 days after 50μg/ml BTH treatments 3 days before inoculation was analyzed by semi-quantitive RT-PCR with the plant’s 18S rRNA as internal control. The results were indicated that 50μg/ml BTH treatment 3 days before inoculation affected WMV, CMV replication but did not affect ZYMV replication.
引文
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