三种养殖扇贝热休克蛋白HSP70在逆境因子下的表达和相关基因的克隆
|
摘要
本文利用免疫学和分子生物学手段对我国三种养殖经济扇贝，栉孔扇贝
（Chlamys farreri）、海湾扇贝（Argopecten irradians）和虾夷扇贝（Mizuhopecten yessoensis）
热休克蛋白 70（Heat Shock Protein 70， HSP70）的诱导表达和基因序列进行
了研究。
应用免疫印迹法（Western immunoblotting），对高温刺激条件下，栉孔扇
贝不同组织中 HSP70 的表达进行了检测。实验结果显示，热休克可以使扇贝鳃组
织中 HSP70 的表达明显升高，但在肌柱、外套膜、性腺中未检测到 HSP70 表达
的变化。
论文首次将 FCM 技术引入扇贝血淋巴细胞 HSP70 表达的研究。2003 年五月，
应用流式细胞术（flow cytometry，FCM)对几种逆境因子（高温、低温、细菌感
染、水质恶化）作用下，栉孔扇贝血淋巴细胞中 HSP70 的表达进行了初步的研究。
实验数据的 t 检验分析结果表明，几种逆境因子对 HSP70 的诱导表达有十分显著
的作用（自由度为 8，P<0.01）。各处理组显著性分析的结果表明（Duncan 检验）：
当水质 COD 为 8.36 时，污染水环境对扇贝的胁迫已接近于肌柱注射 20μl 鳗弧
菌（Vibrio anguillarum）菌液（109cells/ml）的胁迫程度；在相同的?t 条件
下，热休克对扇贝 HSP70 诱导表达的效果比冷休克更为显著。 2003 年 10 月，
应用 FCM 对栉孔扇贝、虾夷扇贝、海湾扇贝在热休克、冷休克条件下 HSP70 的诱
导表达进行监测和比较。t 检验分析结果表明，热休克、冷休克对扇贝 HSP70 诱
导表达的效果十分显著（自由度为 8，P<0.01）。Duncan 检验结果表明：在相同
的?t 条件下，热休克、冷休克对海湾扇贝 HSP70 诱导表达的差异性十分显著；
但对栉孔扇贝、虾夷扇贝 HSP70 诱导表达的差异性不显著。栉孔扇贝春季和秋季
实验结果的差异，反映出栉孔扇贝热胁迫阈值有随季节变化而变化的现象。采用
FCM 对热休克条件下扇贝血淋巴细胞中 HSP70 表达量进行跟踪监测，结果显示，
高温刺激后，扇贝血淋巴细胞中 HSP70 的表达量逐渐升高，在诱导 7h 后达到最
高，其后逐渐下降。
1
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆
博士学位论文
利用 PCR，3’-RACE 和 5’-RACE 技术，克隆了虾夷扇贝、海湾扇贝 HSP70
的基因序列（Partial Sequence Genbank Accession Number：AY435154，
AY435155）。其中虾夷扇贝蛋白质编码区（coding sequence CDS）全长为 2071bp，
编码 657 个氨基酸；海湾扇贝 CDS 全长为 2077bp，编码 659 个氨基酸；两种扇
贝 HSP70 氨基酸序列有 92.7%的同源性。
This study reported the variational expression of a heat shock protein
70( HSP70）in three species of cultured scallop, Chlamys farreri, Argopecten
irradians and Mizuhopecten yessoensis using immunological method. The sequences
of HSP70 cDNA from Argopecten irradians and Mizuhopecten yessoensis were also
defined in this paper.
By western immunoblotting，the effect of thermal stress on the expression of
HSP70 was obtained in various tissues such as hemolymph, muscle, gonad and gills.
The results showed that HSP70 was prominently induced in gills, but not in
hemolymph, muscle and gonad while exposed to heat shock.
In the present study, flow cytometry (FCM) was for the first time introduced to
monitor HSP70 expression in hemolymph cells of scallops. In May 2003, hemolymph
cells were collected from Chlamys farreri undergoing environmental stresses (heat,
cold, bacteria infection and degradation).T-test showed that all those adverse factors
remarkably enhanced the production of HSP70（DF=8，P<0.01）.Duncan-test showed
that: the blight of degradation environment (COD=8.36) approximated to injection
with 20μlVibrio anguillarum（109cells/ml）; heat aroused more notable increase of
HSP70 than cold under the same ?t. Same experiments were carried out in Oct.2003
for all three species Chlamys farreri, Argopecten irradians and Mizuhopecten
yessoensis. Duncan-test showed that heat aroused more remarkable increase of HSP70
3
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
than cold under the same ?t in Argopecten irradians. But in Chlamys farreri and
Mizuhopecten yessoensis, the difference of effect was not distinct between those two
stresses, heat and cold. Different results between spring and fall suggested that the
threshold induction temperature was changed with season in Chlamys farreri. FCM
was applied to track the induction of HSP70 in hemolymph cells of scallops after the
treat of heat shock. The analysis of these data led to the following observation: the
HSP70 concentration in hemolymph cell was found to increase after heat stress;the
maximum appeared at 7 hours later; and then decreased gradually.
The HSP70 cDNAs were cloned by PCR，3’-RACE and 5’-RACE techniques
from the gill tissues of Argopecten irradian and Mizuhopecten yessoensis（Partial
Sequence Genbank Accession Number：AY435154，AY435155）。There is a coding
sequence( CDS)of 2071 nucleotide acids coding 657amino acids in the sequence of
HSP70 cDNA from Mizuhopecten yessoensis; and in the sequence of HSP70 cDNA
from Argopecten irradians, the span of CDS is 2077 nucleotide acids coding
657amino acids. There is a 92.7% identity between HSP70 from Argopecten irradian
and Mizuhopecten yessoensis in the amino acids sequence.
（Chlamys farreri）、海湾扇贝（Argopecten irradians）和虾夷扇贝（Mizuhopecten yessoensis）
热休克蛋白 70（Heat Shock Protein 70， HSP70）的诱导表达和基因序列进行
了研究。
应用免疫印迹法（Western immunoblotting），对高温刺激条件下，栉孔扇
贝不同组织中 HSP70 的表达进行了检测。实验结果显示，热休克可以使扇贝鳃组
织中 HSP70 的表达明显升高，但在肌柱、外套膜、性腺中未检测到 HSP70 表达
的变化。
论文首次将 FCM 技术引入扇贝血淋巴细胞 HSP70 表达的研究。2003 年五月，
应用流式细胞术（flow cytometry，FCM)对几种逆境因子（高温、低温、细菌感
染、水质恶化）作用下，栉孔扇贝血淋巴细胞中 HSP70 的表达进行了初步的研究。
实验数据的 t 检验分析结果表明，几种逆境因子对 HSP70 的诱导表达有十分显著
的作用（自由度为 8，P<0.01）。各处理组显著性分析的结果表明（Duncan 检验）：
当水质 COD 为 8.36 时，污染水环境对扇贝的胁迫已接近于肌柱注射 20μl 鳗弧
菌（Vibrio anguillarum）菌液（109cells/ml）的胁迫程度；在相同的?t 条件
下，热休克对扇贝 HSP70 诱导表达的效果比冷休克更为显著。 2003 年 10 月，
应用 FCM 对栉孔扇贝、虾夷扇贝、海湾扇贝在热休克、冷休克条件下 HSP70 的诱
导表达进行监测和比较。t 检验分析结果表明，热休克、冷休克对扇贝 HSP70 诱
导表达的效果十分显著（自由度为 8，P<0.01）。Duncan 检验结果表明：在相同
的?t 条件下，热休克、冷休克对海湾扇贝 HSP70 诱导表达的差异性十分显著；
但对栉孔扇贝、虾夷扇贝 HSP70 诱导表达的差异性不显著。栉孔扇贝春季和秋季
实验结果的差异，反映出栉孔扇贝热胁迫阈值有随季节变化而变化的现象。采用
FCM 对热休克条件下扇贝血淋巴细胞中 HSP70 表达量进行跟踪监测，结果显示，
高温刺激后，扇贝血淋巴细胞中 HSP70 的表达量逐渐升高，在诱导 7h 后达到最
高，其后逐渐下降。
1
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆
博士学位论文
利用 PCR，3’-RACE 和 5’-RACE 技术，克隆了虾夷扇贝、海湾扇贝 HSP70
的基因序列（Partial Sequence Genbank Accession Number：AY435154，
AY435155）。其中虾夷扇贝蛋白质编码区（coding sequence CDS）全长为 2071bp，
编码 657 个氨基酸；海湾扇贝 CDS 全长为 2077bp，编码 659 个氨基酸；两种扇
贝 HSP70 氨基酸序列有 92.7%的同源性。
This study reported the variational expression of a heat shock protein
70( HSP70）in three species of cultured scallop, Chlamys farreri, Argopecten
irradians and Mizuhopecten yessoensis using immunological method. The sequences
of HSP70 cDNA from Argopecten irradians and Mizuhopecten yessoensis were also
defined in this paper.
By western immunoblotting，the effect of thermal stress on the expression of
HSP70 was obtained in various tissues such as hemolymph, muscle, gonad and gills.
The results showed that HSP70 was prominently induced in gills, but not in
hemolymph, muscle and gonad while exposed to heat shock.
In the present study, flow cytometry (FCM) was for the first time introduced to
monitor HSP70 expression in hemolymph cells of scallops. In May 2003, hemolymph
cells were collected from Chlamys farreri undergoing environmental stresses (heat,
cold, bacteria infection and degradation).T-test showed that all those adverse factors
remarkably enhanced the production of HSP70（DF=8，P<0.01）.Duncan-test showed
that: the blight of degradation environment (COD=8.36) approximated to injection
with 20μlVibrio anguillarum（109cells/ml）; heat aroused more notable increase of
HSP70 than cold under the same ?t. Same experiments were carried out in Oct.2003
for all three species Chlamys farreri, Argopecten irradians and Mizuhopecten
yessoensis. Duncan-test showed that heat aroused more remarkable increase of HSP70
3
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
than cold under the same ?t in Argopecten irradians. But in Chlamys farreri and
Mizuhopecten yessoensis, the difference of effect was not distinct between those two
stresses, heat and cold. Different results between spring and fall suggested that the
threshold induction temperature was changed with season in Chlamys farreri. FCM
was applied to track the induction of HSP70 in hemolymph cells of scallops after the
treat of heat shock. The analysis of these data led to the following observation: the
HSP70 concentration in hemolymph cell was found to increase after heat stress;the
maximum appeared at 7 hours later; and then decreased gradually.
The HSP70 cDNAs were cloned by PCR，3’-RACE and 5’-RACE techniques
from the gill tissues of Argopecten irradian and Mizuhopecten yessoensis（Partial
Sequence Genbank Accession Number：AY435154，AY435155）。There is a coding
sequence( CDS)of 2071 nucleotide acids coding 657amino acids in the sequence of
HSP70 cDNA from Mizuhopecten yessoensis; and in the sequence of HSP70 cDNA
from Argopecten irradians, the span of CDS is 2077 nucleotide acids coding
657amino acids. There is a 92.7% identity between HSP70 from Argopecten irradian
and Mizuhopecten yessoensis in the amino acids sequence.
引文
宝福凯,柳爱华.热休克蛋白的生物医学功能. 生命科学,1997,9(2):71-73
陈胜，徐耘，肖成峰等.苯作业工人血浆热应激蛋白水平研究.中国工业医学杂
志.1999,12(5):260-262
范 云 霞 , 赵 玫 等 . 热 休 克 蛋 白 70 对 突 变 型 p53 稳 定 性 的 影 响 . 癌
症,2001,20(2):131-134
方伯言.热休克蛋白－肽复合物（HSP－PCs）肿瘤疫苗的研究进展. 锦州医学院
学报,2001,22(4):58-60
高雅娟.热休克蛋白与肿瘤免疫. 国外医学:免疫学分册,2000,23(5):290-293
谷文萍.热休克蛋白 70 研究进展. 国外医学:神经病学.神经外科学分
册,1999,26(2):57-59
贾后军.热休克蛋白 70 与内毒素耐受性. 创伤外科杂志,1999,1(2):124-125
孟颂东 高福. 热休克蛋白-多肽复合物在肿瘤和传染性疾病免疫中的作用. 生
物工程学报,2000,16(4):425-428
秦海宏,郭俊生.微量元素对热应激果蝇存活率及热应激蛋白合成的影响. 解放
军预防医学杂志,2000,18(3):164-166
邱璐,郭俊生. 泛酸钙,吡哆醛,叶酸及生物素对热暴露果蝇的保护作用. 解放军
预防医学杂志,2000,18(2);82-85
邱璐,郭俊生. 维生素 A,E 对热暴露果蝇保护作用的研究.解放军预防医学杂
志,1999,17(3)：169-172
沈关心,周汝麟.现代免疫学实验技术(第二版).武汉:湖北科学技术出版
社.2002:226-264
王枫,郭俊生. HSP70 高表达对 K562 细胞热耐力的影响.中国公共卫
生,2000,16(7)：587-588
王枫，赵法及，郭俊生.谷氨酸对果蝇热耐力和Hsp70蛋白含量的影响. 卫生研
究,1998, 279(6):394-395
王缚鲲 张卫军等.幽门螺杆菌热休克蛋白 A 亚单位基因工程菌的发酵. 中国生
物制品学杂志,2001,14(2):84-86
王 万 银 . HSP27 研 究 现 状 . 国 外 医 学 : 生 理 病 理 科 学 与 临 床 分
册,2001,21(6):467-469
王艳林.热休克因子研究进展.湖北三峡学院学报,1997,19(1):110-114
魏 征 人 . 分 子 伴 侣 疫 苗 抗 肿 瘤 的 研 究 进 展 . 中 国 肿 瘤 生 物 治 疗 杂
志.2001,8(1):70-71
相建海等.海水养殖生物病害发生与控制.北京:海洋出版社, 2001. 176-188.
熊蕾 徐存拴. 热休克蛋白(HSPs)在胚胎发育中的作用研究进展. 生物学杂
志,2000,17(2):1-3
张旭辉. 热休克蛋白 70 与耐受的机制. 解放军预防医学杂志, 2001, 19(1):
90
曲凌云 参 考 文 献 博士学位论文
73-75
张玉秀,柴团耀. HSP70 分子伴侣系统研究进展.生物化学与生物物理进
展,1999,26(6):554-558
赵克森,金丽娟.病理生理学.北京:人民军医出版社,1999.107-108.
郑春福.热休克蛋白在传染病中的免疫保护作用. 国外医学:流行病学传染病学
分册,2000,27(4):149-152
郑磊,颜晓慧. 热应激时热休克蛋白 70 及其细胞保护作用.国外医学:卫生学分
册,1999,26(4):209-212
Abravaya K, Phillips B, Morimoto RI. Heat shock-induced interactions of heat shock
transcription factor and the human hsp70 promoter examined by in vivo
footprinting .Mol Cell Biol., 1991, 11(1)：586-592
Adams CC , Gross DS . The yeast heat shock response is induced by conversion of
cells to spheroplasts and by potent transcriptional inhibitors.J Bacteriol 1991,
173: 7429–7435.
Agarraberes, F.A., Terlecky, S.R., and Dice, J.F. An intra-lysosomal hsp70 is required
for a selective pathway of lysosomal protein degradation. J. Cell Biol.,1997,
137：825 834
Ahn S G , Liu P C.C., Klyachko K. et al. The loop domain of heat shock transcription
factor 1 dictates DNA-binding specificity and responses to heat stress. Genes
& Dev. 2001, 15: 2134-2145.
Arnold, D., S. Faath, H.-G. Rammensee, H. Schild. Cross priming of minor
histocompatibility antigen-specific cytotoxic T cells upon immunization with
the heat shock protein gp96. J. Exp. Med. 1995, 182:885-892
Ary A. Hoffmann, Jesper G. Sorensen1, Volker Loeschcke1. Adaptation of
Drosophila to temperature extremes: bringing together quantitative and
molecular approaches. Journal of Thermal Biology ,2003,28:175–216
Astumian, R.D. Thermodynamics and kinetics of a Brownian motor. Science, 1997,
276, 917–922
Banchereau, J., R. M. Steinman. Dendritic cells and the control of immunity. Nature
1998, 392:245 -253
Barba, D., J. Hardin, M. Sadelain, F. H. Gage. Development of anti tumor immunity
following thymidine kinase-mediated killing of experimental brain tumors.
Proc. Natl. Acad. Sci. USA 1994, 91:4348-4356
Barlough JE, Reubel GH, Madigan JE et al. Detection of Ehrlichia risticii, the agent
of Potomac horse fever, in freshwater stream snails (Pleuroceridae: Juga spp.)
from northern California.Appl Environ Microbiol. 1998, 64(8):2888-2893.
Bateman, A., Bullough, F., Murphy, S., et al. Fusogenic Membrane Glycoproteins As
a Novel Class of Genes for the Local and Immune-mediated Control of Tumor
Growth. Cancer Res ,2000,60: 1492-1497
91
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Bauer, M.F., Hofmann, S., Neupert, W. et al. Protein translocation into mitochondria:
the role of TIM complexes. Trends Cell Biol., 2000,10, 25–31.
Beckmann, R.P., Mizzen, L.A., and Welch, W.J. Interaction of HSP70 newly
synthesized proteins: Implications for protein folding and assembly events.
Science, 1990, 248：850-854
Bellmann, K., Burkart, V., Bruckhoff, J., et al. p38-dependent Enhancement of
Cytokine-induced Nitric-oxide Synthase Gene Expression by Heat Shock
Protein 70. J. Biol. Chem. 2000 ,275: 18172-18179
Berwin, B., Reed, R. C., Nicchitta, C. V. Virally Induced Lytic Cell Death Elicits the
Release of Immunogenic GRP94/gp96. J. Biol. Chem. 2001, 276:
21083-21088
Bienz, M and Pelham HR. Mechanisms of heat shock gene activation in higher
eukaryotes. Adv. Genet., 1987, 24: 31-72
Blachere NE, Li Z, Chandawarkar RY, et al. Heat shock protein-peptide complexes,
reconstituted in vitro, elicit peptide-specific cytotoxic T lymphocyte response
and tumor immunity. J Exp Med. 1997,186(8):1315-1322..
B?mer, U., Maarse, A.C., Martin, F., et al. Separation of structural and dynamic
functions of the mitochondrial translocase: Tim44 is crucial for the inner
membrane import sites in translocation of tightly folded domains, but not of
loosely folded preproteins. EMBO J., 1998, 17, 4226–4237
Bonner J. J., Chen D., Storey K., et al. Structural analysis of yeast HSF by
site-specific crosslinking J. Mol. Bio., 2000, 302(3)：581-592
Bonorino C, Nardi NB, Zhang X, Wysocki LJ.，Characteristics of the strong antibody
response to mycobacterial Hsp70: a primary, T cell-dependent IgG response
with no evidence of natural priming or gamma delta T cell involvement. J
Immunol.1998，161（10）：5210-5216
Boon, T., P. van der Bruggen. Human tumor antigens recognized by T lymphocytes. J.
Exp. Med. 1996,183:725-732
Boon-Niermeijer EK, Souren JE, De Waal AM, Van Wijk R.Thermotolerance induced
by heat and ethanol. Int J Hyperthermia. 1988, 4(2):211-222.
Boon-Niermeijer EK, Souren JE, Van Wijk R.Thermotolerance induced by
2,4-dinitrophenol. Int J Hyperthermia. 1987 , 3(2):133-141.
Boon-Niermeijer EK. Heat shock effects in snail development. Results Probl Cell
Differ. 1991,17:7-28.
Borkovich KA , Farrelly FW , Finkelstein DB , Taulien J , Lindquist S : Hsp82 is an
essential protein that is required in higher concentrations for growth of cells at
higher temperatures.Mol Cell Biol ,1989, 9: 3919–3930.
Boulo V, Cadoret JP, Le Marrec F, Dorange G, Miahle E.Transient expression of
luciferase reporter gene after lipofection in oyster (Crassostrea gigas) primary
cell cultures. Mol Mar Biol Biotechnol. 1996, 5(3):167-174.
Boutet I, Tanguy A, Moraga D. Organization and nucleotide sequence of the European
92
曲凌云 参 考 文 献 博士学位论文
flat oyster Ostrea edulis heat shock cognate 70 (hsc70) and heat shock protein
70 (hsp70) genes. Aquat Toxicol. 2003 , 65(2):221-225.
Boutet I, Tanguy A, Rousseau S, Auffret M, Moraga D. Molecular identification and
expression of heat shock cognate 70 (hsc70) and heat shock protein 70 (hsp70)
genes in the Pacific oyster Crassostrea gigas. Cell Stress Chaperones. 2003 ,
8(1):76-85
Brown C R，Doxsey S J，Hong-Brown L Q，et al. Molecular chaperones and the
centrosone. J Biol Chem,1996,271(2):824-832
Bryan PJ, Steffan RJ, DePaola A, Foster JW, Bej AK. Adaptive response to cold
temperatures in Vibrio vulnificus.Curr Microbiol. 1999 , 38(3):168-175.
Buckley BA, Owen ME, Hofmann GE. Adjusting the thermostat: the threshold
induction temperature for the heat-shock response in intertidal mussels (genus
Mytilus) changes as a function of thermal history. J Exp Biol. 2001,
204(20):3571-3579.
Bukau, B. and Horwich, A.L. The Hsp70 and Hsp60 chaperone machines. Cell, 1998,
92, 351–366.
Cadoret JP, Boulo V, Gendreau S, Mialhe E. Promoters from Drosophila heat shock
protein and cytomegalovirus drive transient expression of luciferase
introduced by particle bombardment into embryos of the oyster Crassostrea
gigas. J Biotechnol. 1997 ,28;56(3):183-189.
Chai Y, Jiang X and Ito Y, et al.. Fate of the mammalian cranial neural crest during
tooth and mandibular morphogenesis. Development. 2000 ,127(8):1671-1679.
Chauwin, J.F., Oster, G. and Glick, B.S. Strong precursor-pore interactions constrain
models for mitochondrial protein import. Biophys. J., 1998,74, 1732–1743.
Cheng E. N, Qutob S., Pavliv M., et al. Hsp 27 is better associated with the expression
of inducible thermotolerance in human pancreatic tumor cell lines than hsp 70,
p53 or p21/waf1/cip1.J. Therm. Bio. , 2002, 27(1)：47-54
Clay JR, Kuzirian A. Trafficking of axonal K+ channels: potential role of Hsc70.J
Neurosci Res. 2002 ,15, 67(6):745-752.
Clegg J.S., Uhlinger K.R., Jackson S.A. Induced thermotolerance and the heat shock
protein–70 family in the Pacific oyster Crassostrea gigas. Molecular Marine
Biology and Biotechnology , 1998, 7(1): 21–30
Colombo, M. P., A. Modesti, G. Parmiani, G. Forni.. Local cytokine availability
elicits tumor rejection and systemic immunity through
granulocyte-T-lymphocyte cross-talk. Cancer Res. 1992, 52:4853-4861.
Corporeau C, Auffret M. In situ hybridisation for flow cytometry: a molecular method
for monitoring stress-gene expression in hemolymph cells of oysters. Aquat
Toxicol. 2003 , 64(4):427-435.
Craig EA . Regulation and function of the HSP70 multigene family of Saccharomyces
cerevisiae. In Stress proteins in Biology and Medicine. 1990: 301–321.
Cruz-Rodriguez LA, Baucum AJ, Soudant P, Chu FL, Hale RC. Effects of PCBs
93
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
sorbed to algal paste and sediments on the stress protein response (HSP70
family) in the eastern oyster, Crassostrea virginica.Mar Environ Res. 2000,
50:341-345.
Cruz-Rodriguez LA, Chu FL. Heat-shock protein (HSP70) response in the eastern
oyster, Crassostrea virginica, exposed to PAHs sorbed to suspended artificial
clay particles and to suspended field contaminated sediments. Aquat Toxicol.
2002, 60:157-168.
Das DK，Engelma RM，Kimura Y. Molecular adaptation of cellular defences
following preconditioning of the heart by repeated ischaemia. Cardiovasc Res,
1993, 27(4):578-565
Delannoy P , Caruthers MH : Detection and characterization of a factor which rescues
spliceosome assembly from a heat-inactivated HeLa cell nuclear extract. Mol
Cell Biol 1991, 11: 3425–3431
DeLuca-Flaherty C, McKay DB, et al. Uncoating protein (hsc70) binds a
conformationally labile domain of clathrin light chain LCa to stimulate ATP
hydrolysis. Cell 1990, 62: 875–887.
Dranoff G E, Jaffee A, Lazenby, et al. Vaccination with irradiated tumor cells
engineered to secrete murine granulocyte macrophage colony stimulating
factor stimulates potent, specific, and long lasting anti-tumor immunity. Proc.
Natl. Acad. Sci. USA, 1993, 90:3539-3543
Drees B L, Grotkopp E K, Nelson H.C. The GCN4 leucine zipper can functionally
substitute for the heat shock transcription factor's trimerization domain. J
Mol Biol. 1997, 273(1)：61-74
Dudley P, Wood C K, Pratt J. R.et al. Developmental regulation of the plant
mitochondrial matrix located HSP70 chaperone and its role in protein import.
FEBS Letters, 1997, 417(3): 321-324
Eilers M and Schatz G. Binding of a specific ligand inhibits import of a purified
precursor protein into mitochondria. Nature, 1986, 322, 228–232.
Feder J H , Rossi J M, Solomon J , Solomon N , Lindquist S. The consequences of
expressing Hsp70 in Drosophila cells at normal temperatures. Genes
Develop,1992, 6: 1402–1413.
Feder M E, Cartano N V, Milos L, Krebs RA, Lindquist L. Effect of engineering
Hsp70 copy number on Hsp70 expression andtolerance of ecologically
relevant heatshock in larvae andpupae of Drosophila melanogaster J. Exp.
Biol. 1996 ,199: 1837–1844.
Fede M E, Hofmann G E. Heat-shock proteins, molecular chaperones, and the stress
response. Annu. Rev.Physiol，1999, 61：243–282.
Feder M E, Krebs R A. Natural and genetic engineering of the heat-shock protein
Hsp70 in Drosophila melanogaster: consequences for thermotolerance. Am.
Zool. 1998,38: 503–517.
Feinstein D L, Galea E, Reis D J. Suppression of glial nitric oxide synthase induction
by heat shock: effects on proteolytic degradation of IkappaB-alpha. Nitric
94
曲凌云 参 考 文 献 博士学位论文
Oxide. 1997,1(2):167-176.
Feng H, ZengY, Whitesell L, Katsanis E. Stressed apoptotic tumor cells express heat
shock proteins and elicit tumor-specific immunity. Blood, 2001, 97:
3505-3512
Finkelman F D, Lees R, Birnbaum W, Gause S C. Morris. Dendritic cells can present
antigen in vivo in a tolerogenic or immunogenic fashion. J. Immunol. 1996,
157:1406-1413
Fiszer-Kierzkowska A, Wysocka A, Jarzab M et al. Structure of gene flanking regions
and functional analysis of sequences upstream of the rat hsp70.1 stress
gene .Biochim Biophys Acta. 2003, 1625(1):77-87
Flaherty KM，Deluca-Flaherty C，Mckay D B. Three dimensional structure of the
ATPase fragment of a 70K heat shock cognate protein. Nature,
1990,346(6285):623-628
Flaherty KM，Wilbanks SM，Deluca-Flaherty C et al. Structural basis of the
70-kilodalton heat shock cognate protein ATP hydrolytic activity. Ⅱ. Structure
of the active site with ADP or ATP bound to wild type or mutant ATPase
fragment. J Biol Chem, 1994, 269(17): 12899-12907
Fortin A, Lagace J, Therien H-M. Trafficking of Surface-linked and Encapsulated
Liposomal Antigens in Macrophages: an Immunocytochemical Study. J.
Histochem. Cytochem. 2001, 49: 1407-1420
Frydenberg J, Pierpaoli M, Loeschcke V. Drosophila melanogaster is polymorphic
for a speci.c repeated(CATA) sequence in the regulatory region of Hsp23.
Gene .1999, 236, 243–250.
Fu Q, Guo R, Yao Z. Generation of cytotoxic T lymphocytes induced with HSP-PC70
derived from tumor cells. Zhonghua Yi Xue Za Zhi, 2000 , 80(4):301-303.
Fünfschilling U and Rospert S. Nascent polypeptide-associated complex stimulates
protein import into yeast mitochondria. Mol. Biol. Cell., 1999,10, 3289–3299.
Fuse T. Alterations in cytokinetics and heat shock protein (70 kDa) expression of glial
cell by hyperthermia. No To Shinkei. 1991, 43(9):843-850.
Gambill B D, Voos W, Kang P J, et al. A dual role for mitochondrial heat shock
protein 70 in membrane translocation of preproteins. J. Cell Biol., 1993, 123,
109–117.
Gao B, Biosca J, Craig E A , et al. Uncoating of coated vesicles by yeast hsp70
proteins. J Biol Chem 1991, 266: 19565–19571.
Garrido C, Ottavi P, and Fromentin A et al. HSP27 as a mediator of confluence-dependent
resistance to cell death induced by anticancer drugs. Cancer Res.
1997,57(13):2661-2667
G?rtner F, B?mer U, Guiard B and Pfanner N. The sorting signal of cytochrome b2
promotes early divergence from the general mitochondrial import pathway and
restricts the unfoldase activity of matrix Hsp70. EMBO J., 1995, 14,
6043–6057.
95
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Gaume B, Klaus C, Ungermann C, et al .Unfolding of preproteins upon import into
mitochondria. EMBO J., 1998, 17, 6497–6507
Gioio AE, Eyman M, Zhang H, et al. Local synthesis of nuclear-encoded
mitochondrial proteins in the presynaptic nerve terminal.J Neurosci Res. 2001,
64(5):447-453.
Giudice G, Sconzo G, Roccheri MC. Studies on heat shock proteins in sea urchin
development. Dev Growth Differ. 1999 , 41(4)：375-380
Glick, B.S. Can hsp70 proteins act as force generating motors? Cell, 1995, 80, 11–14.
Glick B S, Wachter C, Reid G A and Schatz G. Import of cytochrome b2 to the
mitochondrial intermembrane space: the tightly folded heme-binding domain
makes import dependent upon matrix ATP. Protein Sci., 1993, 2:1901–1917
Golumbek P T, Azhai E M, Jaffee H I, et al. Controlled release, biodegradable
cytokine depots: a new approach in cancer vaccine design. Cancer Res. 1993,
53:5841-5849
Gomez F J, Gomez M, Deepe G S. An 80-kilodalton antigen from histoplasma
capsulatum that has homology to heat shock protein 70 induces cell-mediated
immune response and protection in mice. Infect Immun,1992,
60(7):2565-2572
Goto S G. Accumulation of Hsp70 mRNA under environmental stresses in diapausing
and nondiapausing adults of Drosophila triauraria. J. Insect Physiol. 1998,
44:1009–1015.
Gottesman S, Squires C , Pichersky E , et al. Conservation of the regulatory subunit
for the CIp ATP-dependent protease in prokaryotes and eukaryotes. Proc Natl
Acad Sci USA 1990, 87: 3513–3517
Gough M J, Melcher A A, Ahmed A, et al. Macrophages Orchestrate the Immune
Response to Tumor Cell Death. Cancer Res 2001, 61: 7240-7247
Gourdon I, Gricourt L, Kellner K, Roch P, Escoubas JM.Characterization of a cDNA
encoding a 72 kDa heat shock cognate protein (Hsc72) from the Pacific oyster,
Crassostrea gigas.DNA Seq. 2000, 11(3):265-270
Guidon PT Jr, Hightower L E. Purification and initial characterization of the
71-kilodalton rat heat-shock protein and its cognate as fatty acid binding
proteins. Biochemistry 1986, 25: 3231–3239.
Gupta R S, Singh B. Bacterrology, Cloning of the HSP70 gene from
Halobacterium marismortui: relatedness of archaebacterial HSP70 to
its eubacterial homologs and a model for the evolution of the HSP70
gene. J Bacteriol. 1992，174：4594-4605
Guzhova IV，Darieva ZA，Melo AR，et al. Major stress protein HSP70 interacts with
NF-κβ regulatory complex in human T-lymphoma cells. Cell Stress
Chaperones,1997, 2(2):132-139
Hall D M, Xu L, Drake V J, et al. Aging reduces adaptive capacity and stress protein
expression in the liver after heat stress. J. Appl. Physiol. 2000, 89: 749-759
96
曲凌云 参 考 文 献 博士学位论文
Hamdoun AM, Cheney DP, Cherr GN. Phenotypic plasticity of HSP70 and HSP70
gene expression in the Pacific oyster (Crassostrea gigas): implications for
thermal limits and induction of thermal tolerance. Biol Bull. 2003 ,
205(2):160-169
Han JH, Yim SW, Lim CS, Park CW, Kaang BK. Expression of a non-inactivating K+
channel driven by a rat heat shock promoter increased the resting potential in
Aplysia silent neurons. Neurosci Res. 1999 May;34(1):13-19.
Hartl F U. Molecular chaperones in cellular protein folding. Nature, 1996, 381,
571–579
He X, You S, Bian S, Liao X, et al. In vitro induction of autologous T cell killing by
heat treated human chronic myelogenous leukemia cells. Zhonghua Xue Ye
Xue Za Zhi. 2000, 21(6):287-90.
Hellstrom K E I, Hellstrom L, Chen. Can co-stimulated tumor immunity be
therapeutically efficacious. Immunol. Rev. 1995, 145:123-129.
Helmuth BS, Hofmann GE. Microhabitats, thermal heterogeneity, and patterns of
physiological stress in the rocky intertidal zone. Biol Bull. 2001,
201(3):374-384.
Hendrick J P, Hartl F U. Molecular chaperone functions of heat-shock proteins. Annu
Rev Biochem，1993, 72:349－384.
Henikoff S , Eghtedarzadeh MK. Conserved arrangement of nested genes at the
Drosophila Gart Locus. Genetics 1987, 117: 711–725
Hill K, Model K, Ryan M T, et al. Tom40 forms the hydrophilic channel of the
mitochondrial import pore for preproteins. Nature, 1998, 395: 516–521
Hock H M, Dorsch U, Kunzendorf K, Uberla Z, Qin T, Diamanstein, T. Blankenstein..
Vaccinations with tumor cells genetically engineered to produce different
cytokines: effectivity not superior to a classical adjuvant. Cancer Res. 1993,
53:714-721
Horwich A L, Weber-Ban E U and Finley D. Chaperone rings in protein folding and
degradation. Proc. Natl Acad. Sci. USA, 1999, 96, 11033–11040
Hotchkiss R，Nunnally I，Lindquist S et al. Hyper thermia protectsmice against the
lethal effects of endotoxin. Am J Physiol,1993, 265(6):1447-1452
Houghton A N. Cancer antigens: immune recognition of self and altered self. J. Exp.
Med. 1994, 180:1-9
Huang A Y, Golumbek M, Ahmadzadeh E, Jaffee D, Pardoll H. Levitsky. Role of
bone marrow derived cells in presenting MHC class I-restricted tumor
antigens. Science 1994, 264:961-972
Huang S, Ratliff K S, Schwartz M P, Spenner J M and Matouschek A. Mitochondria
unfold precursor proteins by unraveling them from their N-termini. Nature
Struct. Biol. 1999, 6:1132–1138.
Hugh R B Pelham，et al. A regulatory upstream promoter element in the drosophila
HSP70 heat shock gene. Cell, 1982, 30:517-528
97
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Hughes P E, Alexi T, Yoshida T et al. Excitotoxic lesion of rat brain with quinolinic
acid induces expression of p53 messenger RNA and protein and p53-inducible
genes bax and Gadd-45 in brain areas showing DNA fragmentation.
Neuroscience, 1996, 74:1143 -1160
Inaba K M, Inaba M, Romani H, Aya et al. Generation of large numbers of dendritic
cells from mouse bone marrow cultures supplemented with granulocyte
macrophage colony stimulating factor. J. Exp. Med. 1992, 176:1693-1701
Jensen R E and Johnson A E. Protein translocation: is Hsp70 pulling my chain? Curr.
Biol., 1999, 9:779–782
Jimenez CR, Eyman M, Lavina ZS, et al. Protein synthesis in synaptosomes: a
proteomics analysis.J Neurochem. 2002 , 81(4):735-744
Johnson KS, Wells K, Bock JV, et al.The 86-kilodalton antigen from Schistosoma
mansoni is a heat-shock protein homologous to yeast HSP-90. Mol Biochem
Parasitol, 1989 ,36(1):19-28
J?rg Martin, F Ulrich Hartl. Chaperone-assisted protein folding .Current Opinion in
Structural Biology, 1997,7(1):41-52
Juliann G, Kiang and George C. Heat shock protein 70 kDa: Molecular Biology,
Biochemistry, and Physiology. Pharmacol. Ther. 1998, 80 (20)：183-201
Kammerer R, Stober D, Riedl P, et al. Noncovalent Association with Stress Protein
Facilitates Cross-Priming of CD8+ T Cells to Tumor Cell Antigens by
Dendritic Cells. The JI 2002, 168: 108-117
Kang P L, Ostermann J, Shilling J, et al. Requirement for hsp70 in the mitochondrial
matrix for translocation and folding of precursor proteins. Nature, 1990, 348:
137–143
Kanwar J R, Kanwar R K, et al. Vascular Attack by 5,6-Dimethylxanthenone
-4-acetic Acid Combined with B7.1 (CD80)-mediated Immunotherapy
Overcomes Immune Resistance and Leads to the Eradication of Large Tumors
and Multiple Tumor Foci. Cancer Res 2001, 61: 1948-1956
Karlin S, Brocchieri L. Heat Shock Protein 70 Family: Multiple Sequence
Comparisons, Function，and Evolution. J Mol Evol, 1998, 47:565–577
Kawazoe Y, Nakai A, Tanabe M, et al. Proteasome inhibition leads to the activation of
all members of the heat-shock-factor family . Eur J Biochem,1998，255(2):356
－362.
Keegstra K and Froehlich J E. Protein import into chloroplasts. Curr. Opin. Plant
Biol., 1999, 2:471–476
Kiang JG, Carr FE, Burns MR, McClain DE. HSP-72 synthesis is promoted by
increase in [Ca2+]i or activation of G proteins but not pHi or cAMP. Am J
Physiol. 1994 ，267：104-114.
Kim YM，De-Vera ME，Watkins SC et al. Nitric oxide protects cultured rat
hepatocytes from tumor necrosis factor-alpha-induced apoptosis by inducing
heat shock protein 70 expression .J.Biol. Chem，1997，272（2）：1402-1411
98
曲凌云 参 考 文 献 博士学位论文
Kim T S, Cohen E P. Interleukin-2 secreting mouse fibroblasts transfected with
genomic DNA from murine melanoma cells prolong the survival of mice with
melanoma. Cancer Res. 1994,54:2531-2538.
Klosterhalfen B，Hauptmann S，Tietze L，et al. The influence of heat shock protein 70
induction on hemodynamic variables in a porcine model of recurrent
endotoxemia. Shock,1997,7(5):358-366
Kotera Y, Shimizu K, Mule J J. Comparative Analysis of Necrotic and Apoptotic
Tumor Cells As a Source of Antigen(s) in Dendritic Cell-based Immunization.
Cancer Res 2001, 61: 8105-8109
Krebs R A, Bettencourt B R. Evolution of thermotolerance andvariation in the heat
shock protein, Hsp70. Am. Zool. 1999,39: 910–919
Krebs RA, Feder M E. Deleterious consequences of Hsp70 over-expression in
Drosophila melanogaster larvae. Cell Stress Chaperones, 1997a, 2(1)：60-71
Krebs RA, Feder M E. Natural variation in the expression of the heat-shock protein
Hsp70 in a population of Drosophila melanogaster and its correlation with
tolerance of ecologically relevant thermal stress. Evolution，1997 b，51：
173–179
Krebs R A, Feder M E. Tissue-special variation in Hsp70 expression and thermal
damage in Drosophila melanogaster larvae. J. Exp. Biol. 1997c, 200：
2007–2015
Krebs RA, Feder M E. Hsp70 and larval thermotolerance in Drosophila melanogaster:
how much is enough and when is more too much? J. Insect Physiol.
1998b,.44:1091–1101
Krebs R A, Roberts S P, et al. Changes in thermotolerance and Hsp70 expression with
domestication in Drosophila melanogaster. J. Evol. Biol. ,2001,14: 75–82.
Kreiling JA, Stephens RE, Kuzirian AM, et al. Polychlorinated biphenyls are
selectively neurotoxic in the developing Spisula solidissima embryo. J Toxicol
Environ Health A. 2000, 61(8):657-675.
Krimmer T, Rassow J, Kunau W H, et al. The mitochondrial protein import motor: the
ATPase domain of matrix Hsp70 is crucial for binding to Tim44, while the
peptide binding domain and the carboxy-terminal segment play a stimulatory
role. Mol. Cell. Biol., 2000, 20: 5879–5887
Kristensen T N, Dahlgaard J, Loeschcke, V. Inbreeding affects the Hsp70 expression
level in two species of Drosophila even at benign temperatures. Evol. Ecol.
Res. , 2002,4:1209–1216.
Kuhl D, Kennedy TE, Barzilai A, Kandel ER.Long-term sensitization training in
Aplysia leads to an increase in the expression of BiP, the major protein
chaperon of the ER.J Cell Biol. 1992 ,119(5):1069-1076.
Künkele, K.P., Heins, S., Dembowski, M., et al. The preprotein translocation channel
of the outer membrane of mitochondria. Cell, 1998,93:1009–1019
99
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Kurtz S , Rossi J , Petko L , Lindquist SL .An ancient developmental induction:
heat-shock proteins induced in sporulation and oogenesis. Science 1986, 231:
1154–1157.
Wood L.A., Brown I.R. and Youson J.H.. Characterisation of the heat shock
response in the gills of sea lampreys and a brook lamprey at
different intervals of their life cycles. Comp. Biochem. Physiol.
1998, 120, 509–518.
Lacoste A, De Cian MC, Cueff A, Poulet SA. Noradrenaline and alpha-adrenergic
signaling induce the hsp70 gene promoter in mollusc immune cells.J Cell Sci.
2001 ,114:3557-3564.
Landschulz W H, Johnson P F, McKnight S L. The leucine zipper: a hypothetical
structure common to a new class of DNA bin-ding proteins.
Science,1988,240(4860):1 759－1764.
Lansing E, Justesen J, Loeschcke V. Variation in the expression of Hsp70, the major
heat-shock protein, and thermotolerance in larval andad ult selection lines of
Drosophila melanogaster. J. Therm. Biol. 2000, 25, 443–450.
Lanuti M, Rudginsky S, Force S D, et al. Cationic Lipid:Bacterial DNA Complexes
Elicit Adaptive Cellular Immunity in Murine Intraperitoneal Tumor Models.
Cancer Res ,2000, 60: 2955-2963
Laursen JR, di Liu H, Wu XJ, Yoshino TP.Heat-shock response in a molluscan cell
line: characterization of the response and cloning of an inducible HSP70
cDNA.J Invertebr Pathol. 1997 ,70(3):226-233.
Lavoie JN, Gingras-Breton G, Tanguay RM, Landry J. Induction of Chinese hamster
HSP27 gene expression in mouse cells confers resistance to heat shock.
HSP27 stabilization of the microfilament organization. J Biol Chem.
1993,268(5):3420-3429
Le Naour F, Hohenkirk L, et al. Profiling Changes in Gene Expression during
Differentiation and Maturation of Monocyte-derived Dendritic Cells Using
Both Oligonucleotide Microarrays and Proteomics. J. Biol. Chem. 2001, 276:
17920-17931
Lerman D N, Feder M E. Laboratory selection at different temperatures modi.es
heat-shock transcription factor (HSF) activation in Drosophila melanogaster. J.
Exp.Biol. 2001, 204, 315–323.
Levitsky H I, A. Lazenby R J, Hayashi D. Pardoll.. In vivo priming of two distinct
antitumor effector populations: the role of MHC class I expression. J. Exp.
Med. 1994, 179:1215-1221
Li GC, Li L, Liu RY , Rehman M , Lee WM . Heat shock protein hsp70 protects cells
from thermal stress even after deletion of its ATP-binding domain.Proc Natl
Acad Sci USA 1992, 89: 2036–2040.
Li Z. Priming of T cells by heat shock protein-peptide complexes as the basis of
tumor vaccines. Semin. Immunol. 1997, 9:315-322
100
曲凌云 参 考 文 献 博士学位论文
Liao XC , Colot HV , Wang Y , Rosbash M : Requirements for U2 snRNP addition to
yeast pre-mRNA.Nucleic Acids Res 1992, 20: 4237–4245.
Liberek K, Skowyra D, Zylicz M, et al. The Escherichia coli DnaK chaperone, the
70-kDa heat shock protein eukaryotic equivalent, changes conformation upon
ATP hydrolysis, thus triggering its dissociation from a bound target protein.
J. Biol. Chem., 1991,266: 14491–14496.
Lima K M, Santos RR, Brandao IT et al, Efficacy of DNA–hsp65 vaccination for
tuberculosis varies with method of DNA introduction in vivo.Vaccine. 2003,
22 (1)：49-56
Lin R, Newman AJ, Cheng S, Abelson J.Yeast mRNA splicing in vitro.J Biol Chem
1985, 260: 14780–14792.
Lindquist S, Craig E A. The heat -shock proteins. Ann Rev Genet, 1988, 22: 631～677
Lindquist S. Translational efficiency of heat-induced messages in Drosophila
melanogaster cells. J Mol Biol. 1980 ,137(2):151-158.
Lindquist S. Varying patterns of protein synthesis in Drosophila during heat shock:
implications for regulation. Dev Biol. 1980，77(2):463-479.
Linquist S. The heat shock response. Annu Rev Chem，1986, 55:1151－1191.
Lithgow T. Targeting of proteins to mitochondria. FEBS Lett., 2000,476: 22–26.
Locke M, Tanguay R M. Increased HSF activation in muscles with a high constitutive
HSP70 expression . Cell Stress Chaperones,1996,1(3):189－195.
Lukacs K V, Nakakes C J, et al.. In vivo gene therapy of malignant tumors with heat
shock protein-65 gene. Gene Ther. 1997, 4:346-353
Lukacs K V, D B Lowrie R W, Stokes M J. Colston.. Tumor cells transfected with a
bacterial heat-shock gene lose tumorigenicity and induce protection against
tumors. J. Exp. Med. 1993, 178:343-349
Lyons C, Dowling V, Tedengren M, et al.Variability of heat shock proteins and
glutathione S-transferase in gill and digestive gland of blue mussel, Mytilus
edulis.Mar Environ Res. 2003,56(5):585-597.
Maass G W, Schmidt M, Berger F, et al. Priming of tumor specific T cells in the
draining lymph nodes after immunization with interleukin 2-secreting tumor
cells: three consecutive stages may be required for successful tumor
vaccination. Proc. Natl. Acad. Sci. USA 1995, 92:5540-5549
Manjili M.H., Wang X.Y., Park J et al. Immunotherapy of cancer using heat shock
proteins. Front Biosci. 2002, 7：43-52
Marber MS, Mestril R, Chi SH. Overexpression of the rat inducible 70-KD heat stress
protein in a transgenic mouse increases the resistance of the heart to ischemic
injury. J. Clin. Invest. 1995,95(4):1446-1453
Margulis BA, Antropova OYu, Kharazova AD.70 kDa heat shock proteins from
mollusc and human cells have common structural and functional
domains.Comp Biochem Physiol B. 1989;94(4):621-623.
101
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Martin J, Mahlke K and Pfanner N. Role of an energized inner membrane in
mitochondrial protein import: drives the movement of presequences. J. Biol.
Chem., 1991, 266, 18051–18057.
Mason PJ, Hall LM and Gausz J. The expression of heat shock genes during normal
development in Drosophila melanogaster. Mol. Gen. Genet., 1984,194: 73-78
Matlack KE, Misselwitz B, Plath K et al. BiP acts as a molecular ratchet during
posttranslational transport of prepro- factor across the ER membrane. Cell,
1999, 97:553–564
Matouschek A, Azem A, Ratliff K, et al. Active unfolding of precursor proteins
during mitochondrial protein import. EMBO J., 1997,16: 6727–6736
Mattingly J R Jr, Iriarte A, et al. Structural features which control folding of
homologous proteins in cell-free translation systems. The effect of a
mitochondrial-targeting presequence on aspartate aminotransferase. J. Biol.
Chem., 1993, 268:26320–26327
Matzinger P. Tolerance, danger and the extended family. Annu. Rev. Immunol. 1994,
12:991998
Matzinger P. An innate sense of danger. Semin. Immunol. 1998,10:399-403
Mehlen P, Schulze-Osthoff K, Arrigo AP. Small stress proteins as novel regulators of
apoptosis, Heat shock protein 27 blocks Fas/APO-1- and
staurosporine-induced cell death. J Biol Chem., 1996, 271(28):16510-16514
Melcher A A S, Todryk N, Hardwick M, Ford M, Jacobson R G Vile. Tumor
immunogenicity is determined by the mechanism of cell death via induction of
heat shock protein expression. Nat. Med. 1998, 4:58-590.
Merlin A, Voos W, Maarse A C, et al.The J-related segment of Tim44 is essential for
cell viability: a mutant Tim44 remains in the mitochondrial import site, but
inefficiently recruits mtHsp70 and impairs protein translocation. J. Cell Biol.,
1999,145:961–972.
Michaud S, Marin R, Tanguay RM.. Regulation of heat shock gene induction and
expression during Drosophila development. Cell Mol Life Sci., 1997，53(1)：
104-113
Mihara K and Omura T. Cytoplasmic chaperones in precursor targeting to
mitochondria: the role of MSF and hsp70. Trends Cell Biol., 1996, 6:104–108.
Mitchell D A, S K Nair E, Gilboa. Dendritic cell/macrophage precursors capture
exogenous antigen for MHC class I presentation by dendritic cells. Eur. J.
Immunol. 1998,28:1923-1928
Morales A V，Haduiargyrou M，et al. Heat shock protein in retinal neurogenesis:
identification of the pMI antigen in comparison to that of other chaperones.
Eur J Neurosci, 1998, 10(10): 3237-3245
Morange M, Diu A, Bensaude O, Babinet C. Altered Expression of Heat Shock
Proteins in Embryonal Carcinoma and Mouse Early Embryonic Cells. Mol.
Cell. Biol., 1984,4:730-735
102
曲凌云 参 考 文 献 博士学位论文
Morimoto R L，Tissieres A. Stress proteins in biology and medicine. Cold Spring
Harbor: CSHL Press, 1990: 1-36
Moro F, Sirrenberg C, Schneider H C, Neupert W and Brunner, M. The TIM17·23
preprotein translocase of mitochondria: composition and function in protein
transport into the matrix. EMBO J., 1999,18, 3667–3675.
Morris J C. Enzyme/Prodrug-Based Tumor Vaccination: All Politics (and Immunity)
Are Local. J Natl Cancer Inst 1999,91: 1986-1989
Muhich ML, Boothroyd JC. Polycistronic transcripts in trypanosomes and their
accumulation during heat shock: evidence for a precursor role in mRNA
synthesis. Mol Cell Biol 1988, 8: 3837–3846.
Mullen C A M M, Coale R, Lowe R M, Blaese. Tumors expressing the cytosine
deaminase suicide gene can be eliminated in vivo with 5-fluorocytosine and
induce protective immunity to wild type tumor. Cancer Res.
1994,54:1503-1509
Multhoff G，Botzler C，Issels R. The role of heat shock protein in the stimulation of an
immune response. Biol Chem., 1998, 379(3): 295-301
Multhoff G C, Botzler L, Jennen J, Schmidt J, Ellwart R Issels. Heat shock protein 72
on tumor cells: a recognition structure for natural killer cells. J. Immunol.
1997,158:4341-4352
Nabel G J A, Chang E G, Nabel G, Plautz B A, Fox L, Huang S Shu. Clinical protocol:
immunotherapy of malignancy by in vivo gene transfer into tumors. Hum.
Gene Ther. 1992,3:399-405
Nakai A, Tanabe M, Kawazoe Y, et al. HSF4, a new member of the human heat shock
factor family which lacks properties of a transcriptional activator. Mol Cell
Biol, 1997, 17(1): 469－481.
Nakanoma T, Ueno M, Iida M, et al Effects of quercetin on the heat-induced
cytotoxicity of prostate cancer cells. Int J Urol. 2001 , 8(11):623-630.
Nathan D F, Lindquist S. Mutational analysis of Hsp90 function: Interactions with a
steroid receptor and protein kinase. Mol Cell Biol, 1995, 15: 3917～3925
Neira J L and Fersht A R. Acquisition of native-like interactions in C-terminal
fragments of barnase. J. Mol. Biol., 1999,287:421–432.
Netzer W and Hartl F. Recombination of protein domains facilitated by
co-translational folding in eukaryotes. Nature, 1997 ,388:343–349.
Neumann S, Ziv E, Lantner F, Schechter I.Regulation of HSP70 gene expression
during the life cycle of the parasitic helminth Schistosoma mansoni.Eur J
Biochem. 1993 ,212(2):589-596.
Neupert W. Protein import into mitochondria. Annu. Rev. Biochem., 1997,66:
863–917
Neupert W, Hartl F U, Craig E A and Pfanner N. How do polypeptides cross the
mitochondrial membranes? Cell, 1990, 63: 447–450.
103
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Newton EM, Knauf U, Green M, et al. The regulatory domain of human heat shock
factor 1 is sufficient to sense heat stress. Mol Cell Biol,1996,16(3):839－846.
Jurivich D, Sistonen L, Kroes R, et al. Effect of sodium sali-cylate on the human heat
shock response. Science,1992, 255(5 049):1 243－1245
Ng R, Abelson J. Isolation and sequence of the gene for actin in Saccharomyces
cerevisiae.Proc Natl Acad Sci USA 1980, 77: 3912–3916.
Ng D T, Brown J D and Walter P. Signal sequences specify the targeting route to the
endoplasmic reticulum membrane. J. Cell Biol., 1996,134:269–278.
Niedzwiecki A, Reveillaud I, Fleming JE. Changes in superoxide dismutasc and
catalase in aging heat-shocked Drosophila. Free Radic Res Commun, 1992,
17(6):355-361
Ochoa GH, Clark YM, Matsumoto B et al. Heat shock protein 70 and heat shock
protein 90 expression in light- and dark-adapted adult octopus retinas. J
Neurocytol. 2002 ,31(2):161-174.
Ohtsuka K.,Suzuki T. Roles of molecular chaperones in the nervous system. Brain
Research Bulletin, 2000, 53(2):141-146
Onozuka M, Watanabe K.Intracellularly applied anti-P70 antibody blocks the
induction of abnormal membrane properties by pentylenetetrazole in identified
Euhadra neurons.Brain Res. 1996 ,716(1):187-191.
Ouellette MM ， Mcdaniel LD ， Wright WE et al. The establishment of
telomerase-immortalized cell lines representing human chromosome
instability syndromes. Hum Mol Gene,2000, 9:403-411
Pardoll D M. Paracrine cytokine adjuvants in cancer immunotherapy. Annu. Rev.
Immunol. 1995,13:399-407
Parsell DA , Kowal AS , Lindquist SL. Saccharomyces cerevisiae Hsp104 protein.J
Biol Chem 1994, 269: 4480–4487
Parsell DA , Kowal AS , Singer M , Lindquist SL. Hsp104-mediated protein
disaggregation: a novel heat-shock protein function. Nature 1994, 372:
475–478.
Parsell DA , Lindquist SL. The function of heat-shock proteins in stress tolerance:
degradation and reactivation of damaged proteins. Annu Rev Genet 1993, 27:
437–496
Parsell DA , Sanchez Y , Stitzel JD , Lindquist S .Hsp104 is a highly conserved
protein with two essential nucleotide-binding sites. Nature 1991, 353:
270–273
Parsell DA, Taulien J, Lindquist S. The role of heat-shock proteins in
thermotolerance.Philos Trans R Soc Lond .1993, 339: 279–285
Paunola E, Suntio T, Jamsa E and Makarow M. Folding of active ?-lactamase in the
yeast cytoplasm before translocation into the endoplasmic reticulum. Mol. Biol.
Cell, 1998,9, 817–827
104
曲凌云 参 考 文 献 博士学位论文
Pelham H R B. A regulatory upstream promoter element in the Drosophila hsp70 heat-
shock gene . Cell，1986,46(5):959－961.
Pfanner N and Meijer M. Pulling in the proteins. Curr. Biol., 1995,5:132–135.
Pfanner N, Craig E A and H?nlinger A. Mitochondrial preprotein translocase. Annu.
Rev. Cell. Dev. Biol., 1997,13, 25–51
Piano A, Asirelli C, Caselli F et al. Hsp70 expression in thermally stressed Ostrea
edulis, a commercially important oyster in Europe. Cell Stress Chaperones.
2002, 7(3)：250-257
Polla BS，Kantengwa S，Franccis D，et al. Mitochondria are selective targets for the
protective effects of heat shock against oxidative injury. Proc Natl Acad Sci
USA,1996,93(13):6458-6463
Porte C, Biosca X, Sole M, Albaiges J. The integrated use of chemical analysis,
cytochrome P450 and stress proteins in mussels to assess pollution along the
Galician coast (NW Spain).Environ Pollut. 2001,112(2):261-268.
Radlowska M, Pempkowiak J.Stress-70 as indicator of heavy metals accumulation in
blue mussel Mytilus edulis. Environ Int. 2002, 27(8):605-608.
Rajasekar R G K, Sim A, Augustin. Self heat shock and T-cell reactivity. Proc. Natl.
Acad. Sci. USA 1990,87:1767-1773
Ramage JM, Young JL, Goodall JC, Gaston JS.T cell responses to heat-shock protein
60: differential responses by CD4+ T cell subsets according to their expression
of CD45 isotypes.J Immunol. 1999,162(2):704-710.
Rassow J, Guiard B, Wienhues U et al. Translocation arrest by reversible folding of a
precursor protein imported into mitochondria. A means to quantitate
translocation contact sites. J. Cell Biol., 1989,109:1421–1428.
Rassow J, Hartl F-U, Guiard B, et al. Polypeptides traverse the mitochondrial
envelope in an extended state. FEBS Lett., 1990, 275:190–194.
Rathinam AV, Chen TT, Grossfeld RM.Cloning and sequence analysis of a cDNA for
an inducible 70 kDa heat shock protein (Hsp70) of the American oyster
(Crassostrea virginica).DNA Seq. 2000,11:261-264.
Reubel GH, Barlough JE, Madigan JE.Production and characterization of Ehrlichia
risticii, the agent of Potomac horse fever, from snails (Pleuroceridae: Juga spp.)
in aquarium culture and genetic comparison to equine strains.J Clin Microbiol.
1998,36(6):1501-1511.
Richard I. Morimoto. Cells in Stress: Transcriptional Activation of Heat Shock Genes.
Science, 1993, 259, (5): 1409-1410
Ritossa F. A new puffing pattern induced by temperature shock and DNP in
Drosophila. Experientia， 1962,18:571－ 573.
Rodolfo M C, Zilocchi C, Melani B, Cappetti I, Arioli G, Parmiani M P.
Immunotherapy of experimental metastases by vaccination with interleukin
gene-transduced adenocarcinoma cells sharing tumor-associated antigens:
105
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
comparison between IL-12 and IL-2 gene-transduced tumor cell vaccines. J.
Immunol. 1996,157:5536-5541
Roy H，Bur Don. Heat shock and the heat shock proteins. Biochem J, 1986, 240：
313-324
Ryan AJ，Flanagan SW，Moseley PL.et al. Acute heat stress protects rates against
endotoxin shock. J Appl Physiol,1992,73(4):1517-1524
S^rensen J G, Loeschcke V. Decreased heat-shock resistance and own-regulation of
Hsp70 expression with increasing age in adult Drosophila melanogaster.
Funct .Ecol. 2002, 16:379–384.
S^rensen, J.G., Loeschcke, V.,. Larval crowding in Drosophila melanogaster induces
Hsp70 expression, and leads to increased adult longevity and adult thermal
stress resistance. J. Insect Physiol. 2001,47:1301–1307.
Samali A, Cotter T G. Heat Shock Proteins Increase Resistance to Apoptosis.
Experimental Cell Research, 1996, 223:163–170
Sanatacruz H, Vriz S, Angelier N. Molecular characterization of a heat shock cognate
cDNA of zebrafish, hsc70, and developmental expression of the corresponding
transcripts. Dev Genetics1997, 21: 223–233.
Sanchez Y, Lindquist SL. HSP104 required for induced thermotolerance. Science
1990, 248: 1112–1115.
Sanchez Y, Parsell DA, Taulien J, et al. Genetic evidence for a functional relationship
between Hsp104 and Hsp70. J Bacteriol 1993, 175: 6484–6491
Sanchez Y, Taulien J , Borkovich KA , Lindquist SL. Hsp104 is required for tolerance
to many forms of stress. EMBO J 1992, 11: 2357–2364
Sanchez Y, Lindquist S.L. HSP104 required for induced thermotolerance. Science,
1990, 248: 1112-1115
Sanders BM, Martin LS, Howe SR, et al. Tissue-specific differences in accumulation
of stress proteins in Mytilus edulis exposed to a range of copper
concentrations.Toxicol Appl Pharmacol. 1994 ,125(2):206-213.
Sanders BM, Martin LS.Stress proteins as biomarkers of contaminant exposure in
archived environmental samples. Sci Total Environ. 1993 ,139:459-470.
Sauter B, Albert M L, Francisco L et al. Consequences of Cell Death: Exposure to
Necrotic Tumor Cells, but Not Primary Tissue Cells or Apoptotic Cells,
Induces the Maturation of Immunostimulatory Dendritic Cells. J. Exp. Med.
2000,191: 423-434
Schatz G and Dobberstein B. Common principles of protein translocation across
membranes. Science, 1996, 271:1519–1526.
Schena M, Picard D, Yamamoto K R.Vectors for constitutive and inducible gene
expression in yeast. In Guide to Yeast Genetics and Molecular Biology. Edited
by Guthrie C, Fink GR. Academic Press, Inc., 1991, 194: 389–398.
Schill RO, Gayle PM, Kohler HR. Daily stress protein (hsp70) cycle in chitons
106
曲凌云 参 考 文 献 博士学位论文
(Acanthopleura granulata Gmelin, 1791) which inhabit the rocky intertidal
shoreline in a tropical ecosystem.Comp Biochem Physiol C Toxicol Pharmacol.
2002 ,131(3):253-258.
Schroder HT, Langer T , Hartl F-U , Bukau B. DnaK, DnaJ, GrpE form a cellular
chaperone machinery capable of repairing heat-induced protein damage.
EMBO J 1993, 12: 4137–4144.
Schwartz M P and Matouschek A. The dimensions of the protein import channels in
the outer and inner mitochondrial membranes. Proc. Natl Acad. Sci. USA,
1999, 96, 13086–13090
Schwartz M P, Huang S and Matouschek A. The structure of precursor proteins
during import into mitochondria. J. Biol. Chem., 1999,274:12759–12764
Schwarz E, Seytter T, Guiard B, et al. Targeting of cytochrome b2 into the
mitochondrial intermembrane space: specific recognition of the sorting signal.
EMBO J., 1993,12: 2295–2302
Shaner SL , Piatt DM , Wensley CG ,et al. Aggregation equilibria of Escherichia coli
RNA polymerase: evidence for anion-linked conformational transitions in the
protomers of core and holoenzyme. Biochemistry 1982, 21: 5539–5551
Shen Z G, Reznikoff G, Dranoff et al. Cloned dendritic cells can present exogenous
antigens on both class I and class II molecules. J. Immunol. 1997,
158:2723-2729
Shi L, Kataoka M and Fink A L. Conformational characterization of DnaK and its
complexes by small-angle X-ray scattering. Biochemistry, 1996, 35:
3297–3308.
Shukla RR , Dominski Z , Zwierzynski T et al. Inactivation of splicing factors in
HeLa cells subjected to heat shock.J Biol Chem 1990, 265: 20377–20383
Simon S M, Peskin C S and Oster G F. What drives the translocation of proteins?
Proc. Natl Acad. Sci. USA, 1992,89:3770–3774.
Sistonen L, Sarge K D, Phillips B, et al. Activation of heat shock factor 2 during
hemin- induced differentiation of human ery-throleukemia cells. Mol Cell Biol,
1992, 12（9）: 4104－4111
Skowyra D, Georgopoulos C , Zylicz M : The E. Coli dnaK gene product, the hsp70
homolog, can reactivate heat-inactivated RNA polymerase in an ATP
hydrolysis-dependent manner. Cell 1990, 62: 939–944
Snyder MJ, Girvetz E, Mulder EP. Induction of marine mollusc stress proteins by
chemical or physical stress.Arch Environ Contam Toxicol. 2001,41(1):22-29.
Sol M, Morcillo Y, Porte C.Stress-protein response in tributyltin-exposed clams. Bull
Environ Contam Toxicol. 2000,64(6):852-858.
Solomon JM, Rossi JM , Golic K et al. Changes in hsp70 alter thermotolerance and
heat-shock regulation in Drosophila. New Biol 1991, 3: 1106–1120
Sorger P K and Pelham H R B. Yeast heat shock factor is an essential DNA- binding
protein that exhibits temperature-dependent phosphorylation. Cell, 1988, 54(6):
107
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
855－864.
Squires C, Squires CL. The CIp proteins: proteolysis regulators or molecular
chaperones. J Bacteriol 1992, 174: 1081–1085
Srivastava PK ， Udono H. Heat shock protein-peptide complexes in cancer
immunotherapy. Curr Opin Immunol. 1994, 6(5): 728-734
Srivastava P K A, Menoret S, Basu R J, Binder K L. McQuade. Heat shock proteins
come of age: primitive functions acquire new roles in an adaptive world.
Immunity 1998,8:657-665
Srivastava P K, Udono H. Heat shock protein-peptide complexes in cancer
immunotherapy. Curr. Opin. Immunol. 1994,6:728-734
Srivastava P H, Udono N E, Blachere Z Li. Heat shock proteins transfer peptides
during antigen processing and CTL priming. Immunogenetics 1994, 39:93-101
Stone DE, Craig EA. Self-regulation of 70-kilodalton heat shock proteins in
Saccharomyces cerevisiae. Mol Cell Biol 1990, 10: 1622–1632.
Sullivan CS，Cantalupo P，Pipas JM. The molecular chaperone activity of simian virus
40 large antigen is required to disrupt Rb-E2F family complexes by an
ATP-dependent mechanism. Mol Cell Biol, 2000,20:6233-6243
Suto R P, Srivastava K. A mechanism for the specific immunogenicity of heat shock
protein-chaperoned peptides. Science 1995,269:1585-1599
Suzue K X, Zhou H N, Eisen R A. Young. Heat shock fusion proteins as vehicles for
antigen delivery into the major histocompatibility complex class I presentation
pathway. Proc. Natl. Acad. Sci. USA 1997, 94:13146-13154
Suzuki H, Kalair W, Shivji G M, Wang B, Toto P, Amerio P, Kraemer K H, Sauder D
N. Impaired Ultraviolet-B-Induced Cytokine Induction in Xeroderma
Pigmentosum Fibroblasts. J Invest Dermatol 2001,117: 1151-1155
Tamura Y P, Peng K, Liu M, Daou P K. Srivastava. Immunotherapy of tumors with
autologous tumor-derived heat shock protein preparations. Science
1997,278:117-129
Tanaka Y, Kobayashi K, Kita M, Kinoshita S, Imanishi J .Messenger RNA expression
of heat shock proteins (HSPs) during ocular development. Curr Eye Res. 1995
14(12):1125-1133.
Tang WB, Ma PQ, Ni H, Zeng WT, Zhu KL. Expression of HSP70 and P-glycoprotein
(P-gp) in human hepatocarcinoma HepG2 cells induced by heat shock and
inhibiting effect of quercetin on them. Ai Zheng. 2003 ,22(9):954-958.
Tatar M. Evolution of senescence: longevity and the expression of heat shock proteins.
Am. Zool. 1999，39：920–927.
Thompson EM, Legouy EL, Christians E, Renard JP. Expression of the HSP70.1 gene,
a landmark of early zygotic activity in the mouse embryo, is restricted to the
first burst of transcription. Development .1995,121:113-122
Tomanek L, Sanford E.Heat-shock protein 70 (Hsp70) as a biochemical stress
108
曲凌云 参 考 文 献 博士学位论文
indicator: an experimental field test in two congeneric intertidal gastropods
(genus: Tegula).Biol Bull. 2003 ,205(3):276-284.
Tomanek L, Somero GN.Interspecific- and acclimation-induced variation in levels of
heat-shock proteins 70 (hsp70) and 90 (hsp90) and heat-shock transcription
factor-1 (HSF1) in congeneric marine snails (genus Tegula): implications for
regulation of hsp gene expression.J Exp Biol. 2002 ,205(5):677-685.
Tomanek L, Somero GN.Time course and magnitude of synthesis of heat-shock
proteins in congeneric marine snails (Genus tegula) from different tidal
heights.Physiol Biochem Zool. 2000 ,73(2):249-256.
Tower J. Transgenic methods for increasing Drosophila life span. Mech Ageing Dev.
2000,118:1-14
Tsdengen M, Olsson B, Reimer J F, et al. Heat pre-treatment increases cadmium
resistance and HSP70 levels in Baltic sea mussels. Aquat. Toxicol.,
2000,48:1-12
Tytell M, Greenberg SG, Lasek RJ.Heat shock-like protein is transferred from glia to
axon. Brain Res. 1986, 363(1):161-164.
Udono H, Saito T, Ogawa M, et al. Hsp-antigen fusion and their use for immunization.
Methods, 2004，32：21-24
Udono H D L, Levey P K Srivastava. Cellular requirements for tumor-specific
immunity elicited by heat shock proteins: tumor rejection antigen gp96 primes
CD8+ T cells in vivo. Proc. Natl. Acad. Sci. USA 1994, 91:3077-3083
Ungermann C, Neupert W and Cyr D M. The role of hsp70 in conferring
unidirectionality on protein translocation into mitochondria. Science, 1994,
266, 1250–1253.
Utans U, Behrens S , Luhrmann R , Kole R , Kramer A. A splicing factor that is
inactivated during in vivo heat shock is functionally equivalent to the
(U4/U6.U5) snRNP-specific proteins.Genes Dev, 1992, 6: 631–641
Vayssier M, Vayssier F, Leguerhier J F, et al. Cloning and analysis of a Trichinella briotovi gene
encoding a cytoplasmic heat shock protein of 72 kDa. Parasitology ,1999, 119:81–93.
Veldhuizen-Tsoerkan MB, Holwerda DA et al. A field study on stress indices in the
sea mussel, Mytilus edulis: application of the "stress approach" in
biomonitoring. Arch Environ Contam Toxicol. 1991, 21(4):497-504.
Veldhuizen-Tsoerkan MB, Holwerda DA,et al. Synthesis of stress proteins under
normal and heat shock conditions in gill tissue of sea mussels (Mytilus edulis)
after chronic exposure to cadmium. Comp Biochem Physiol C.
1991,100(3):699-706.
Vestweber D and Schatz G. DNA–protein conjugates can enter mitochondria via the
protein import pathway. Nature, 1989, 338, 170–172.
Vijayraghavan U, Company M, Abelson J. Isolation and characterization of
pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 1989, 3:
1206–1216
109
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Vile R G, Chong H. Immunotherapy: combinatorial molecular immunotherapy: a
synthesis and suggestions. Cancer Metastasis Rev. 1996,15:351-359
Vile R G, Hart I R. In vitro and in vivo targeting of gene expression to melanoma
cells. Cancer Res. 1993,53:962-970
Vile R G, Nelson J A, Castleden S, Chong H, Hart I R. Systemic gene therapy of
murine melanoma using tissue specific expression of the HSVtk gene involves
an immune component. Cancer Res. 1994,54:6228-6233
Vile R G, Castleden S C, Marshall J, Camplejohn R, Upton C, Chong H. Generation
of an anti-tumor immune response in a non-immunogenic tumor:
HSVtk-killing in vivo stimulates a mononuclear cell infiltrate and a Th1-like
profile of intratumoral cytokine expression. Int. J. Cancer 1997,71:267-274
Virca GD, Northemann W , Shiels BR et al. Simplified northern blot hybridization
using 5 % sodium dodecyl sulfate. Biotechniques 1990, 8: 370–371
Voisine C, Craig EA, Zufall N, et al. The protein import motor of mitochondria:
unfolding and trapping of preproteins are distinct and separable functions of
matrix Hsp70. Cell, 1999,97:565–574.
Von Ahsen O, Voos W, Henninger H and Pfanner N. The mitochondrial protein
import machinery: role of ATP in dissociation of the Hsp70–Mim44 complex.
J. Biol. Chem., 1995,270, 29848–29853
Voos W, Gambill B D, Guiard B et al. Presequence and mature part of preproteins
strongly influence the dependence of mitochondrial protein import on heat
shock protein 70 in the matrix. J. Cell Biol., 1993, 123, 119–126
Voos W, Martin H, Krimmer T and Pfanner N. Mechanisms of protein translocation
into mitochondria. Biochim. Biophys. Acta, 1999, 1422: 235–254
Voos W, von Ahsen O, Müller H, et al. Differential requirement for the mitochondrial
Hsp70–Tim44 complex in unfolding and translocation of preproteins. EMBO
J., 1996,15:2668–2677
Wachter C, Schatz G and Glick B S. Protein import into mitochondria: the
requirement for external ATP is precursor-specific whereas intramitochondrial
ATP is universally needed for translocation into the matrix. Mol. Biol. Cell,
1994, 5:465–474
Wadhwa R，Yaguchi T. HSP70 family member, mot-2/mthsp70/grp75, hinds to the
cytoplasmic sequestration domain of the P53 Protein. Cell Res, 2002, 274:
246-253
Walker D, Chaddock A M, Chaddock J A, et al. Ricin A chain fused to a
chloroplast-targeting signal is unfolded on the chloroplast surface prior to
import across the envelope membranes. J. Biol. Chem., 1996 ,271: 4082–4085
Wang F, Li B, Yang B, Yu F. Effects of heat shock protein 70 overexpression on
apoptosis of K562 cell caused by hyperthermia. Wei Sheng Yan Jiu. 2001
30(2):65-67.
Wang X Y, Manjili M H and Chen X, et al. Development of cancer vaccines using
autologous and recombinant high molecular weight stress proteins .Methods,
110
曲凌云 参 考 文 献 博士学位论文
2004，32(1)：13-20
Watanabe N, Tsuji N, Kabayashi D, et al. Endogenous tumor necrosis factor functions
as a resistant factor against hyperthermic cytotoxicity in pancreatic carcinoma
cells via enhancement of the heat shock element- binding activity of heat
shock factor 1 .Chemotherapy,1997,43(6):406－414.
Wei Y -q, Zhao X, Kariya Y, Fukata H, Teshigawara K, Uchida A. Induction of
autologous tumor killing by heat treatment of fresh human tumor cells:
involvement of yd T cells and heat shock protein 70. Cancer Res.
1996,56:1104-1112
Wells AD, Malkovsky M. Heat shock proteins, tumor immunogenicity and antigen
presentation: an integrated view. Immunol Today, 2000, 21(3): 129-133
Wells AD, Rai S K, S M, et al. Hsp-72-mediated augmentation of MHC class I
surface expression and endogenous antigen presentation. Int. Immunol. ,1997,
10:609-615
Welte MA, Tetrault JM, Dellavalle RP, Lindquist SL .A new method for manipulating
transgenes: engineering heat tolerance in a complex, multicellular organism.
Curr Biol 1993, 3: 842–853.
Wendling U，Bloemendal A，Van Der Zee R et al. Entirheumatic E. coli. Extract
OM-89 induces T cell response to HSP60 and 70. Int J Immunopharmacol,
1997, 19: 565-575
Werner I, Hinton DE.Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula
amurensis) in northern San Francisco Bay may be linked to natural rather than
anthropogenic stressors. Mar Environ Res. 2000，50(1):379-384.
Werner-Washburne M , Stone DE , Craig EA. Complex interactions among members
of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae.Mol
Cell Biol 1987, 7: 2568–2577.
Wickner S, Gottesman S, Skowyra D, et al. A molecular chaperone, ClpA, functions
like DnaK and DnaJ.Proc Natl Acad Sci USA 1994, 91: 12218–12222.
Wickner S , Hoskins J , McKenney K. Monomerization of RepA dimers by heat shock
proteins activates binding to DNA replication origin.Proc Natl Acad Sci
USA ,1991, 88: 7903–7907.
Wienhues U, Becker K, Schleyer M et al.Protein folding causes an arrest of preprotein
translocation into mitochondria in vivo. J. Cell Biol., 1991,115:1601–1609
Wu C. Activating protein factor binds in vitro to upstream control sequences in heat
shock gene chromatin . Nature，1984,311 (5981):81－84.
Wu WZ, Liu KD, Xie Q, Wu HS. The study on the expression of membrane HSP70
protein in H22 cell and its immunoprotective mechanism against carcinoma. J
Exp Clin Cancer Res. 1999, 18(4):543-548.
Xiao H, Perisic O, Lis JT. Cooperative binding of Drosophila heat shock factor to
arrays of a conserved 5 bp unit. Cell. 1991, 64(3):585-593
Yem A W, Tomasselli A G and Heinrikson R L. The Hsp56 component of steroid
111
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
receptor complexes binds to immobilized FK506 and shows homology to
FKBP-12 and FKBP-13.J. Biol. Chem., 1992, 267(5)：2868 -2871
Yoshino TP, Wu XJ, Liu HD. Transfection and heat-inducible expression of molluscan
promoter-luciferase reporter gene constructs in the Biomphalaria glabrata
embryonic snail cell line. Am J Trop Med Hyg. 1998,59(3):414-420.
Yost HJ, Lindquist S . RNA splicing is interrupted by heat shock and is rescued by
heat shock protein synthesis. Cell 1986, 45: 185–193.
Yost HJ, Lindquist S. Translation of unspliced transcripts after heat shock. Science
1988, 242: 1544–1548.
Yost HJ , Lindquist SL. Heat shock proteins affect RNA processing during the heat
shock response of Saccharomyces cerevisiae.Mol Cell Biol 1991, 11:
1062–1068
Yost HJ , Petersen RB , Lindquist SL. RNA metabolism: strategies for regulation in
the heat shock response. Trends Genet 1990, 6: 223–227
Yura T, Nagai H, Mori H. Regulation of the heat－shock response in bacteria. Annu
Rev Microbiol，1993,47:321－350.
Zhang Y, Frejtag W, Dai R. et al. Heat shock factor-4 (HSF-4a) is a repressor of
HSF-1 mediated transcription. J Cell Biochem. 2001, 82(4)：692-703
Zhu X, Zhao X, Burkholder W F, et al. Structural analysis of substrate binding by the
molecular chaperone Dnak. Science, 1996, 272(5268): 1606-1614
Ziemienowicz A , Skowyra D , Zeilstra-Ryalls J , Fayet O , Georgopoulos C , Zylicz
M. Both the Escherichia coli chaperone systems, GroEL/GroES and
Dnak/Dnaj/GrpE, can reactivate heattreated RNA polymerase. J Biol Chem
1993, 268: 25425–25431
Zimmerman J L，Petri W，Meselson M. Accumulation of specific subset of
Drosophila melanogaster mRNA in normal development without heat shock.
Cell, 1983, 32:1161-1170
Zugel U, Kaufmann SH. Activation of CD8 T cells with specificity for mycobacterial heat
shock protein 60 in Mycobacterium bovis bacillus Calmette-Guerin-vaccinated mice.
Infect Immun. 1997,65(9): 3947-3950
Zugel U，Kaufmann SH. Immune response against heat shock protein in infectious
diseases. Innunobiology, 1999, 201(1): 22-27
Zugel U，Kaufmann SH. Role of heat shock proteins in protection from pathogensis of
infectious diseases. Clin Microbiol Rev, 1999, 12(1):19-24
Zylicz M. The Escherichia coli chaperones involved in DNA replication.Philos Trans
R Soc Lond [Biol] 1993, 339: 271–277.
陈胜，徐耘，肖成峰等.苯作业工人血浆热应激蛋白水平研究.中国工业医学杂
志.1999,12(5):260-262
范 云 霞 , 赵 玫 等 . 热 休 克 蛋 白 70 对 突 变 型 p53 稳 定 性 的 影 响 . 癌
症,2001,20(2):131-134
方伯言.热休克蛋白－肽复合物（HSP－PCs）肿瘤疫苗的研究进展. 锦州医学院
学报,2001,22(4):58-60
高雅娟.热休克蛋白与肿瘤免疫. 国外医学:免疫学分册,2000,23(5):290-293
谷文萍.热休克蛋白 70 研究进展. 国外医学:神经病学.神经外科学分
册,1999,26(2):57-59
贾后军.热休克蛋白 70 与内毒素耐受性. 创伤外科杂志,1999,1(2):124-125
孟颂东 高福. 热休克蛋白-多肽复合物在肿瘤和传染性疾病免疫中的作用. 生
物工程学报,2000,16(4):425-428
秦海宏,郭俊生.微量元素对热应激果蝇存活率及热应激蛋白合成的影响. 解放
军预防医学杂志,2000,18(3):164-166
邱璐,郭俊生. 泛酸钙,吡哆醛,叶酸及生物素对热暴露果蝇的保护作用. 解放军
预防医学杂志,2000,18(2);82-85
邱璐,郭俊生. 维生素 A,E 对热暴露果蝇保护作用的研究.解放军预防医学杂
志,1999,17(3)：169-172
沈关心,周汝麟.现代免疫学实验技术(第二版).武汉:湖北科学技术出版
社.2002:226-264
王枫,郭俊生. HSP70 高表达对 K562 细胞热耐力的影响.中国公共卫
生,2000,16(7)：587-588
王枫，赵法及，郭俊生.谷氨酸对果蝇热耐力和Hsp70蛋白含量的影响. 卫生研
究,1998, 279(6):394-395
王缚鲲 张卫军等.幽门螺杆菌热休克蛋白 A 亚单位基因工程菌的发酵. 中国生
物制品学杂志,2001,14(2):84-86
王 万 银 . HSP27 研 究 现 状 . 国 外 医 学 : 生 理 病 理 科 学 与 临 床 分
册,2001,21(6):467-469
王艳林.热休克因子研究进展.湖北三峡学院学报,1997,19(1):110-114
魏 征 人 . 分 子 伴 侣 疫 苗 抗 肿 瘤 的 研 究 进 展 . 中 国 肿 瘤 生 物 治 疗 杂
志.2001,8(1):70-71
相建海等.海水养殖生物病害发生与控制.北京:海洋出版社, 2001. 176-188.
熊蕾 徐存拴. 热休克蛋白(HSPs)在胚胎发育中的作用研究进展. 生物学杂
志,2000,17(2):1-3
张旭辉. 热休克蛋白 70 与耐受的机制. 解放军预防医学杂志, 2001, 19(1):
90
曲凌云 参 考 文 献 博士学位论文
73-75
张玉秀,柴团耀. HSP70 分子伴侣系统研究进展.生物化学与生物物理进
展,1999,26(6):554-558
赵克森,金丽娟.病理生理学.北京:人民军医出版社,1999.107-108.
郑春福.热休克蛋白在传染病中的免疫保护作用. 国外医学:流行病学传染病学
分册,2000,27(4):149-152
郑磊,颜晓慧. 热应激时热休克蛋白 70 及其细胞保护作用.国外医学:卫生学分
册,1999,26(4):209-212
Abravaya K, Phillips B, Morimoto RI. Heat shock-induced interactions of heat shock
transcription factor and the human hsp70 promoter examined by in vivo
footprinting .Mol Cell Biol., 1991, 11(1)：586-592
Adams CC , Gross DS . The yeast heat shock response is induced by conversion of
cells to spheroplasts and by potent transcriptional inhibitors.J Bacteriol 1991,
173: 7429–7435.
Agarraberes, F.A., Terlecky, S.R., and Dice, J.F. An intra-lysosomal hsp70 is required
for a selective pathway of lysosomal protein degradation. J. Cell Biol.,1997,
137：825 834
Ahn S G , Liu P C.C., Klyachko K. et al. The loop domain of heat shock transcription
factor 1 dictates DNA-binding specificity and responses to heat stress. Genes
& Dev. 2001, 15: 2134-2145.
Arnold, D., S. Faath, H.-G. Rammensee, H. Schild. Cross priming of minor
histocompatibility antigen-specific cytotoxic T cells upon immunization with
the heat shock protein gp96. J. Exp. Med. 1995, 182:885-892
Ary A. Hoffmann, Jesper G. Sorensen1, Volker Loeschcke1. Adaptation of
Drosophila to temperature extremes: bringing together quantitative and
molecular approaches. Journal of Thermal Biology ,2003,28:175–216
Astumian, R.D. Thermodynamics and kinetics of a Brownian motor. Science, 1997,
276, 917–922
Banchereau, J., R. M. Steinman. Dendritic cells and the control of immunity. Nature
1998, 392:245 -253
Barba, D., J. Hardin, M. Sadelain, F. H. Gage. Development of anti tumor immunity
following thymidine kinase-mediated killing of experimental brain tumors.
Proc. Natl. Acad. Sci. USA 1994, 91:4348-4356
Barlough JE, Reubel GH, Madigan JE et al. Detection of Ehrlichia risticii, the agent
of Potomac horse fever, in freshwater stream snails (Pleuroceridae: Juga spp.)
from northern California.Appl Environ Microbiol. 1998, 64(8):2888-2893.
Bateman, A., Bullough, F., Murphy, S., et al. Fusogenic Membrane Glycoproteins As
a Novel Class of Genes for the Local and Immune-mediated Control of Tumor
Growth. Cancer Res ,2000,60: 1492-1497
91
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Bauer, M.F., Hofmann, S., Neupert, W. et al. Protein translocation into mitochondria:
the role of TIM complexes. Trends Cell Biol., 2000,10, 25–31.
Beckmann, R.P., Mizzen, L.A., and Welch, W.J. Interaction of HSP70 newly
synthesized proteins: Implications for protein folding and assembly events.
Science, 1990, 248：850-854
Bellmann, K., Burkart, V., Bruckhoff, J., et al. p38-dependent Enhancement of
Cytokine-induced Nitric-oxide Synthase Gene Expression by Heat Shock
Protein 70. J. Biol. Chem. 2000 ,275: 18172-18179
Berwin, B., Reed, R. C., Nicchitta, C. V. Virally Induced Lytic Cell Death Elicits the
Release of Immunogenic GRP94/gp96. J. Biol. Chem. 2001, 276:
21083-21088
Bienz, M and Pelham HR. Mechanisms of heat shock gene activation in higher
eukaryotes. Adv. Genet., 1987, 24: 31-72
Blachere NE, Li Z, Chandawarkar RY, et al. Heat shock protein-peptide complexes,
reconstituted in vitro, elicit peptide-specific cytotoxic T lymphocyte response
and tumor immunity. J Exp Med. 1997,186(8):1315-1322..
B?mer, U., Maarse, A.C., Martin, F., et al. Separation of structural and dynamic
functions of the mitochondrial translocase: Tim44 is crucial for the inner
membrane import sites in translocation of tightly folded domains, but not of
loosely folded preproteins. EMBO J., 1998, 17, 4226–4237
Bonner J. J., Chen D., Storey K., et al. Structural analysis of yeast HSF by
site-specific crosslinking J. Mol. Bio., 2000, 302(3)：581-592
Bonorino C, Nardi NB, Zhang X, Wysocki LJ.，Characteristics of the strong antibody
response to mycobacterial Hsp70: a primary, T cell-dependent IgG response
with no evidence of natural priming or gamma delta T cell involvement. J
Immunol.1998，161（10）：5210-5216
Boon, T., P. van der Bruggen. Human tumor antigens recognized by T lymphocytes. J.
Exp. Med. 1996,183:725-732
Boon-Niermeijer EK, Souren JE, De Waal AM, Van Wijk R.Thermotolerance induced
by heat and ethanol. Int J Hyperthermia. 1988, 4(2):211-222.
Boon-Niermeijer EK, Souren JE, Van Wijk R.Thermotolerance induced by
2,4-dinitrophenol. Int J Hyperthermia. 1987 , 3(2):133-141.
Boon-Niermeijer EK. Heat shock effects in snail development. Results Probl Cell
Differ. 1991,17:7-28.
Borkovich KA , Farrelly FW , Finkelstein DB , Taulien J , Lindquist S : Hsp82 is an
essential protein that is required in higher concentrations for growth of cells at
higher temperatures.Mol Cell Biol ,1989, 9: 3919–3930.
Boulo V, Cadoret JP, Le Marrec F, Dorange G, Miahle E.Transient expression of
luciferase reporter gene after lipofection in oyster (Crassostrea gigas) primary
cell cultures. Mol Mar Biol Biotechnol. 1996, 5(3):167-174.
Boutet I, Tanguy A, Moraga D. Organization and nucleotide sequence of the European
92
曲凌云 参 考 文 献 博士学位论文
flat oyster Ostrea edulis heat shock cognate 70 (hsc70) and heat shock protein
70 (hsp70) genes. Aquat Toxicol. 2003 , 65(2):221-225.
Boutet I, Tanguy A, Rousseau S, Auffret M, Moraga D. Molecular identification and
expression of heat shock cognate 70 (hsc70) and heat shock protein 70 (hsp70)
genes in the Pacific oyster Crassostrea gigas. Cell Stress Chaperones. 2003 ,
8(1):76-85
Brown C R，Doxsey S J，Hong-Brown L Q，et al. Molecular chaperones and the
centrosone. J Biol Chem,1996,271(2):824-832
Bryan PJ, Steffan RJ, DePaola A, Foster JW, Bej AK. Adaptive response to cold
temperatures in Vibrio vulnificus.Curr Microbiol. 1999 , 38(3):168-175.
Buckley BA, Owen ME, Hofmann GE. Adjusting the thermostat: the threshold
induction temperature for the heat-shock response in intertidal mussels (genus
Mytilus) changes as a function of thermal history. J Exp Biol. 2001,
204(20):3571-3579.
Bukau, B. and Horwich, A.L. The Hsp70 and Hsp60 chaperone machines. Cell, 1998,
92, 351–366.
Cadoret JP, Boulo V, Gendreau S, Mialhe E. Promoters from Drosophila heat shock
protein and cytomegalovirus drive transient expression of luciferase
introduced by particle bombardment into embryos of the oyster Crassostrea
gigas. J Biotechnol. 1997 ,28;56(3):183-189.
Chai Y, Jiang X and Ito Y, et al.. Fate of the mammalian cranial neural crest during
tooth and mandibular morphogenesis. Development. 2000 ,127(8):1671-1679.
Chauwin, J.F., Oster, G. and Glick, B.S. Strong precursor-pore interactions constrain
models for mitochondrial protein import. Biophys. J., 1998,74, 1732–1743.
Cheng E. N, Qutob S., Pavliv M., et al. Hsp 27 is better associated with the expression
of inducible thermotolerance in human pancreatic tumor cell lines than hsp 70,
p53 or p21/waf1/cip1.J. Therm. Bio. , 2002, 27(1)：47-54
Clay JR, Kuzirian A. Trafficking of axonal K+ channels: potential role of Hsc70.J
Neurosci Res. 2002 ,15, 67(6):745-752.
Clegg J.S., Uhlinger K.R., Jackson S.A. Induced thermotolerance and the heat shock
protein–70 family in the Pacific oyster Crassostrea gigas. Molecular Marine
Biology and Biotechnology , 1998, 7(1): 21–30
Colombo, M. P., A. Modesti, G. Parmiani, G. Forni.. Local cytokine availability
elicits tumor rejection and systemic immunity through
granulocyte-T-lymphocyte cross-talk. Cancer Res. 1992, 52:4853-4861.
Corporeau C, Auffret M. In situ hybridisation for flow cytometry: a molecular method
for monitoring stress-gene expression in hemolymph cells of oysters. Aquat
Toxicol. 2003 , 64(4):427-435.
Craig EA . Regulation and function of the HSP70 multigene family of Saccharomyces
cerevisiae. In Stress proteins in Biology and Medicine. 1990: 301–321.
Cruz-Rodriguez LA, Baucum AJ, Soudant P, Chu FL, Hale RC. Effects of PCBs
93
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
sorbed to algal paste and sediments on the stress protein response (HSP70
family) in the eastern oyster, Crassostrea virginica.Mar Environ Res. 2000,
50:341-345.
Cruz-Rodriguez LA, Chu FL. Heat-shock protein (HSP70) response in the eastern
oyster, Crassostrea virginica, exposed to PAHs sorbed to suspended artificial
clay particles and to suspended field contaminated sediments. Aquat Toxicol.
2002, 60:157-168.
Das DK，Engelma RM，Kimura Y. Molecular adaptation of cellular defences
following preconditioning of the heart by repeated ischaemia. Cardiovasc Res,
1993, 27(4):578-565
Delannoy P , Caruthers MH : Detection and characterization of a factor which rescues
spliceosome assembly from a heat-inactivated HeLa cell nuclear extract. Mol
Cell Biol 1991, 11: 3425–3431
DeLuca-Flaherty C, McKay DB, et al. Uncoating protein (hsc70) binds a
conformationally labile domain of clathrin light chain LCa to stimulate ATP
hydrolysis. Cell 1990, 62: 875–887.
Dranoff G E, Jaffee A, Lazenby, et al. Vaccination with irradiated tumor cells
engineered to secrete murine granulocyte macrophage colony stimulating
factor stimulates potent, specific, and long lasting anti-tumor immunity. Proc.
Natl. Acad. Sci. USA, 1993, 90:3539-3543
Drees B L, Grotkopp E K, Nelson H.C. The GCN4 leucine zipper can functionally
substitute for the heat shock transcription factor's trimerization domain. J
Mol Biol. 1997, 273(1)：61-74
Dudley P, Wood C K, Pratt J. R.et al. Developmental regulation of the plant
mitochondrial matrix located HSP70 chaperone and its role in protein import.
FEBS Letters, 1997, 417(3): 321-324
Eilers M and Schatz G. Binding of a specific ligand inhibits import of a purified
precursor protein into mitochondria. Nature, 1986, 322, 228–232.
Feder J H , Rossi J M, Solomon J , Solomon N , Lindquist S. The consequences of
expressing Hsp70 in Drosophila cells at normal temperatures. Genes
Develop,1992, 6: 1402–1413.
Feder M E, Cartano N V, Milos L, Krebs RA, Lindquist L. Effect of engineering
Hsp70 copy number on Hsp70 expression andtolerance of ecologically
relevant heatshock in larvae andpupae of Drosophila melanogaster J. Exp.
Biol. 1996 ,199: 1837–1844.
Fede M E, Hofmann G E. Heat-shock proteins, molecular chaperones, and the stress
response. Annu. Rev.Physiol，1999, 61：243–282.
Feder M E, Krebs R A. Natural and genetic engineering of the heat-shock protein
Hsp70 in Drosophila melanogaster: consequences for thermotolerance. Am.
Zool. 1998,38: 503–517.
Feinstein D L, Galea E, Reis D J. Suppression of glial nitric oxide synthase induction
by heat shock: effects on proteolytic degradation of IkappaB-alpha. Nitric
94
曲凌云 参 考 文 献 博士学位论文
Oxide. 1997,1(2):167-176.
Feng H, ZengY, Whitesell L, Katsanis E. Stressed apoptotic tumor cells express heat
shock proteins and elicit tumor-specific immunity. Blood, 2001, 97:
3505-3512
Finkelman F D, Lees R, Birnbaum W, Gause S C. Morris. Dendritic cells can present
antigen in vivo in a tolerogenic or immunogenic fashion. J. Immunol. 1996,
157:1406-1413
Fiszer-Kierzkowska A, Wysocka A, Jarzab M et al. Structure of gene flanking regions
and functional analysis of sequences upstream of the rat hsp70.1 stress
gene .Biochim Biophys Acta. 2003, 1625(1):77-87
Flaherty KM，Deluca-Flaherty C，Mckay D B. Three dimensional structure of the
ATPase fragment of a 70K heat shock cognate protein. Nature,
1990,346(6285):623-628
Flaherty KM，Wilbanks SM，Deluca-Flaherty C et al. Structural basis of the
70-kilodalton heat shock cognate protein ATP hydrolytic activity. Ⅱ. Structure
of the active site with ADP or ATP bound to wild type or mutant ATPase
fragment. J Biol Chem, 1994, 269(17): 12899-12907
Fortin A, Lagace J, Therien H-M. Trafficking of Surface-linked and Encapsulated
Liposomal Antigens in Macrophages: an Immunocytochemical Study. J.
Histochem. Cytochem. 2001, 49: 1407-1420
Frydenberg J, Pierpaoli M, Loeschcke V. Drosophila melanogaster is polymorphic
for a speci.c repeated(CATA) sequence in the regulatory region of Hsp23.
Gene .1999, 236, 243–250.
Fu Q, Guo R, Yao Z. Generation of cytotoxic T lymphocytes induced with HSP-PC70
derived from tumor cells. Zhonghua Yi Xue Za Zhi, 2000 , 80(4):301-303.
Fünfschilling U and Rospert S. Nascent polypeptide-associated complex stimulates
protein import into yeast mitochondria. Mol. Biol. Cell., 1999,10, 3289–3299.
Fuse T. Alterations in cytokinetics and heat shock protein (70 kDa) expression of glial
cell by hyperthermia. No To Shinkei. 1991, 43(9):843-850.
Gambill B D, Voos W, Kang P J, et al. A dual role for mitochondrial heat shock
protein 70 in membrane translocation of preproteins. J. Cell Biol., 1993, 123,
109–117.
Gao B, Biosca J, Craig E A , et al. Uncoating of coated vesicles by yeast hsp70
proteins. J Biol Chem 1991, 266: 19565–19571.
Garrido C, Ottavi P, and Fromentin A et al. HSP27 as a mediator of confluence-dependent
resistance to cell death induced by anticancer drugs. Cancer Res.
1997,57(13):2661-2667
G?rtner F, B?mer U, Guiard B and Pfanner N. The sorting signal of cytochrome b2
promotes early divergence from the general mitochondrial import pathway and
restricts the unfoldase activity of matrix Hsp70. EMBO J., 1995, 14,
6043–6057.
95
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Gaume B, Klaus C, Ungermann C, et al .Unfolding of preproteins upon import into
mitochondria. EMBO J., 1998, 17, 6497–6507
Gioio AE, Eyman M, Zhang H, et al. Local synthesis of nuclear-encoded
mitochondrial proteins in the presynaptic nerve terminal.J Neurosci Res. 2001,
64(5):447-453.
Giudice G, Sconzo G, Roccheri MC. Studies on heat shock proteins in sea urchin
development. Dev Growth Differ. 1999 , 41(4)：375-380
Glick, B.S. Can hsp70 proteins act as force generating motors? Cell, 1995, 80, 11–14.
Glick B S, Wachter C, Reid G A and Schatz G. Import of cytochrome b2 to the
mitochondrial intermembrane space: the tightly folded heme-binding domain
makes import dependent upon matrix ATP. Protein Sci., 1993, 2:1901–1917
Golumbek P T, Azhai E M, Jaffee H I, et al. Controlled release, biodegradable
cytokine depots: a new approach in cancer vaccine design. Cancer Res. 1993,
53:5841-5849
Gomez F J, Gomez M, Deepe G S. An 80-kilodalton antigen from histoplasma
capsulatum that has homology to heat shock protein 70 induces cell-mediated
immune response and protection in mice. Infect Immun,1992,
60(7):2565-2572
Goto S G. Accumulation of Hsp70 mRNA under environmental stresses in diapausing
and nondiapausing adults of Drosophila triauraria. J. Insect Physiol. 1998,
44:1009–1015.
Gottesman S, Squires C , Pichersky E , et al. Conservation of the regulatory subunit
for the CIp ATP-dependent protease in prokaryotes and eukaryotes. Proc Natl
Acad Sci USA 1990, 87: 3513–3517
Gough M J, Melcher A A, Ahmed A, et al. Macrophages Orchestrate the Immune
Response to Tumor Cell Death. Cancer Res 2001, 61: 7240-7247
Gourdon I, Gricourt L, Kellner K, Roch P, Escoubas JM.Characterization of a cDNA
encoding a 72 kDa heat shock cognate protein (Hsc72) from the Pacific oyster,
Crassostrea gigas.DNA Seq. 2000, 11(3):265-270
Guidon PT Jr, Hightower L E. Purification and initial characterization of the
71-kilodalton rat heat-shock protein and its cognate as fatty acid binding
proteins. Biochemistry 1986, 25: 3231–3239.
Gupta R S, Singh B. Bacterrology, Cloning of the HSP70 gene from
Halobacterium marismortui: relatedness of archaebacterial HSP70 to
its eubacterial homologs and a model for the evolution of the HSP70
gene. J Bacteriol. 1992，174：4594-4605
Guzhova IV，Darieva ZA，Melo AR，et al. Major stress protein HSP70 interacts with
NF-κβ regulatory complex in human T-lymphoma cells. Cell Stress
Chaperones,1997, 2(2):132-139
Hall D M, Xu L, Drake V J, et al. Aging reduces adaptive capacity and stress protein
expression in the liver after heat stress. J. Appl. Physiol. 2000, 89: 749-759
96
曲凌云 参 考 文 献 博士学位论文
Hamdoun AM, Cheney DP, Cherr GN. Phenotypic plasticity of HSP70 and HSP70
gene expression in the Pacific oyster (Crassostrea gigas): implications for
thermal limits and induction of thermal tolerance. Biol Bull. 2003 ,
205(2):160-169
Han JH, Yim SW, Lim CS, Park CW, Kaang BK. Expression of a non-inactivating K+
channel driven by a rat heat shock promoter increased the resting potential in
Aplysia silent neurons. Neurosci Res. 1999 May;34(1):13-19.
Hartl F U. Molecular chaperones in cellular protein folding. Nature, 1996, 381,
571–579
He X, You S, Bian S, Liao X, et al. In vitro induction of autologous T cell killing by
heat treated human chronic myelogenous leukemia cells. Zhonghua Xue Ye
Xue Za Zhi. 2000, 21(6):287-90.
Hellstrom K E I, Hellstrom L, Chen. Can co-stimulated tumor immunity be
therapeutically efficacious. Immunol. Rev. 1995, 145:123-129.
Helmuth BS, Hofmann GE. Microhabitats, thermal heterogeneity, and patterns of
physiological stress in the rocky intertidal zone. Biol Bull. 2001,
201(3):374-384.
Hendrick J P, Hartl F U. Molecular chaperone functions of heat-shock proteins. Annu
Rev Biochem，1993, 72:349－384.
Henikoff S , Eghtedarzadeh MK. Conserved arrangement of nested genes at the
Drosophila Gart Locus. Genetics 1987, 117: 711–725
Hill K, Model K, Ryan M T, et al. Tom40 forms the hydrophilic channel of the
mitochondrial import pore for preproteins. Nature, 1998, 395: 516–521
Hock H M, Dorsch U, Kunzendorf K, Uberla Z, Qin T, Diamanstein, T. Blankenstein..
Vaccinations with tumor cells genetically engineered to produce different
cytokines: effectivity not superior to a classical adjuvant. Cancer Res. 1993,
53:714-721
Horwich A L, Weber-Ban E U and Finley D. Chaperone rings in protein folding and
degradation. Proc. Natl Acad. Sci. USA, 1999, 96, 11033–11040
Hotchkiss R，Nunnally I，Lindquist S et al. Hyper thermia protectsmice against the
lethal effects of endotoxin. Am J Physiol,1993, 265(6):1447-1452
Houghton A N. Cancer antigens: immune recognition of self and altered self. J. Exp.
Med. 1994, 180:1-9
Huang A Y, Golumbek M, Ahmadzadeh E, Jaffee D, Pardoll H. Levitsky. Role of
bone marrow derived cells in presenting MHC class I-restricted tumor
antigens. Science 1994, 264:961-972
Huang S, Ratliff K S, Schwartz M P, Spenner J M and Matouschek A. Mitochondria
unfold precursor proteins by unraveling them from their N-termini. Nature
Struct. Biol. 1999, 6:1132–1138.
Hugh R B Pelham，et al. A regulatory upstream promoter element in the drosophila
HSP70 heat shock gene. Cell, 1982, 30:517-528
97
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Hughes P E, Alexi T, Yoshida T et al. Excitotoxic lesion of rat brain with quinolinic
acid induces expression of p53 messenger RNA and protein and p53-inducible
genes bax and Gadd-45 in brain areas showing DNA fragmentation.
Neuroscience, 1996, 74:1143 -1160
Inaba K M, Inaba M, Romani H, Aya et al. Generation of large numbers of dendritic
cells from mouse bone marrow cultures supplemented with granulocyte
macrophage colony stimulating factor. J. Exp. Med. 1992, 176:1693-1701
Jensen R E and Johnson A E. Protein translocation: is Hsp70 pulling my chain? Curr.
Biol., 1999, 9:779–782
Jimenez CR, Eyman M, Lavina ZS, et al. Protein synthesis in synaptosomes: a
proteomics analysis.J Neurochem. 2002 , 81(4):735-744
Johnson KS, Wells K, Bock JV, et al.The 86-kilodalton antigen from Schistosoma
mansoni is a heat-shock protein homologous to yeast HSP-90. Mol Biochem
Parasitol, 1989 ,36(1):19-28
J?rg Martin, F Ulrich Hartl. Chaperone-assisted protein folding .Current Opinion in
Structural Biology, 1997,7(1):41-52
Juliann G, Kiang and George C. Heat shock protein 70 kDa: Molecular Biology,
Biochemistry, and Physiology. Pharmacol. Ther. 1998, 80 (20)：183-201
Kammerer R, Stober D, Riedl P, et al. Noncovalent Association with Stress Protein
Facilitates Cross-Priming of CD8+ T Cells to Tumor Cell Antigens by
Dendritic Cells. The JI 2002, 168: 108-117
Kang P L, Ostermann J, Shilling J, et al. Requirement for hsp70 in the mitochondrial
matrix for translocation and folding of precursor proteins. Nature, 1990, 348:
137–143
Kanwar J R, Kanwar R K, et al. Vascular Attack by 5,6-Dimethylxanthenone
-4-acetic Acid Combined with B7.1 (CD80)-mediated Immunotherapy
Overcomes Immune Resistance and Leads to the Eradication of Large Tumors
and Multiple Tumor Foci. Cancer Res 2001, 61: 1948-1956
Karlin S, Brocchieri L. Heat Shock Protein 70 Family: Multiple Sequence
Comparisons, Function，and Evolution. J Mol Evol, 1998, 47:565–577
Kawazoe Y, Nakai A, Tanabe M, et al. Proteasome inhibition leads to the activation of
all members of the heat-shock-factor family . Eur J Biochem,1998，255(2):356
－362.
Keegstra K and Froehlich J E. Protein import into chloroplasts. Curr. Opin. Plant
Biol., 1999, 2:471–476
Kiang JG, Carr FE, Burns MR, McClain DE. HSP-72 synthesis is promoted by
increase in [Ca2+]i or activation of G proteins but not pHi or cAMP. Am J
Physiol. 1994 ，267：104-114.
Kim YM，De-Vera ME，Watkins SC et al. Nitric oxide protects cultured rat
hepatocytes from tumor necrosis factor-alpha-induced apoptosis by inducing
heat shock protein 70 expression .J.Biol. Chem，1997，272（2）：1402-1411
98
曲凌云 参 考 文 献 博士学位论文
Kim T S, Cohen E P. Interleukin-2 secreting mouse fibroblasts transfected with
genomic DNA from murine melanoma cells prolong the survival of mice with
melanoma. Cancer Res. 1994,54:2531-2538.
Klosterhalfen B，Hauptmann S，Tietze L，et al. The influence of heat shock protein 70
induction on hemodynamic variables in a porcine model of recurrent
endotoxemia. Shock,1997,7(5):358-366
Kotera Y, Shimizu K, Mule J J. Comparative Analysis of Necrotic and Apoptotic
Tumor Cells As a Source of Antigen(s) in Dendritic Cell-based Immunization.
Cancer Res 2001, 61: 8105-8109
Krebs R A, Bettencourt B R. Evolution of thermotolerance andvariation in the heat
shock protein, Hsp70. Am. Zool. 1999,39: 910–919
Krebs RA, Feder M E. Deleterious consequences of Hsp70 over-expression in
Drosophila melanogaster larvae. Cell Stress Chaperones, 1997a, 2(1)：60-71
Krebs RA, Feder M E. Natural variation in the expression of the heat-shock protein
Hsp70 in a population of Drosophila melanogaster and its correlation with
tolerance of ecologically relevant thermal stress. Evolution，1997 b，51：
173–179
Krebs R A, Feder M E. Tissue-special variation in Hsp70 expression and thermal
damage in Drosophila melanogaster larvae. J. Exp. Biol. 1997c, 200：
2007–2015
Krebs RA, Feder M E. Hsp70 and larval thermotolerance in Drosophila melanogaster:
how much is enough and when is more too much? J. Insect Physiol.
1998b,.44:1091–1101
Krebs R A, Roberts S P, et al. Changes in thermotolerance and Hsp70 expression with
domestication in Drosophila melanogaster. J. Evol. Biol. ,2001,14: 75–82.
Kreiling JA, Stephens RE, Kuzirian AM, et al. Polychlorinated biphenyls are
selectively neurotoxic in the developing Spisula solidissima embryo. J Toxicol
Environ Health A. 2000, 61(8):657-675.
Krimmer T, Rassow J, Kunau W H, et al. The mitochondrial protein import motor: the
ATPase domain of matrix Hsp70 is crucial for binding to Tim44, while the
peptide binding domain and the carboxy-terminal segment play a stimulatory
role. Mol. Cell. Biol., 2000, 20: 5879–5887
Kristensen T N, Dahlgaard J, Loeschcke, V. Inbreeding affects the Hsp70 expression
level in two species of Drosophila even at benign temperatures. Evol. Ecol.
Res. , 2002,4:1209–1216.
Kuhl D, Kennedy TE, Barzilai A, Kandel ER.Long-term sensitization training in
Aplysia leads to an increase in the expression of BiP, the major protein
chaperon of the ER.J Cell Biol. 1992 ,119(5):1069-1076.
Künkele, K.P., Heins, S., Dembowski, M., et al. The preprotein translocation channel
of the outer membrane of mitochondria. Cell, 1998,93:1009–1019
99
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Kurtz S , Rossi J , Petko L , Lindquist SL .An ancient developmental induction:
heat-shock proteins induced in sporulation and oogenesis. Science 1986, 231:
1154–1157.
Wood L.A., Brown I.R. and Youson J.H.. Characterisation of the heat shock
response in the gills of sea lampreys and a brook lamprey at
different intervals of their life cycles. Comp. Biochem. Physiol.
1998, 120, 509–518.
Lacoste A, De Cian MC, Cueff A, Poulet SA. Noradrenaline and alpha-adrenergic
signaling induce the hsp70 gene promoter in mollusc immune cells.J Cell Sci.
2001 ,114:3557-3564.
Landschulz W H, Johnson P F, McKnight S L. The leucine zipper: a hypothetical
structure common to a new class of DNA bin-ding proteins.
Science,1988,240(4860):1 759－1764.
Lansing E, Justesen J, Loeschcke V. Variation in the expression of Hsp70, the major
heat-shock protein, and thermotolerance in larval andad ult selection lines of
Drosophila melanogaster. J. Therm. Biol. 2000, 25, 443–450.
Lanuti M, Rudginsky S, Force S D, et al. Cationic Lipid:Bacterial DNA Complexes
Elicit Adaptive Cellular Immunity in Murine Intraperitoneal Tumor Models.
Cancer Res ,2000, 60: 2955-2963
Laursen JR, di Liu H, Wu XJ, Yoshino TP.Heat-shock response in a molluscan cell
line: characterization of the response and cloning of an inducible HSP70
cDNA.J Invertebr Pathol. 1997 ,70(3):226-233.
Lavoie JN, Gingras-Breton G, Tanguay RM, Landry J. Induction of Chinese hamster
HSP27 gene expression in mouse cells confers resistance to heat shock.
HSP27 stabilization of the microfilament organization. J Biol Chem.
1993,268(5):3420-3429
Le Naour F, Hohenkirk L, et al. Profiling Changes in Gene Expression during
Differentiation and Maturation of Monocyte-derived Dendritic Cells Using
Both Oligonucleotide Microarrays and Proteomics. J. Biol. Chem. 2001, 276:
17920-17931
Lerman D N, Feder M E. Laboratory selection at different temperatures modi.es
heat-shock transcription factor (HSF) activation in Drosophila melanogaster. J.
Exp.Biol. 2001, 204, 315–323.
Levitsky H I, A. Lazenby R J, Hayashi D. Pardoll.. In vivo priming of two distinct
antitumor effector populations: the role of MHC class I expression. J. Exp.
Med. 1994, 179:1215-1221
Li GC, Li L, Liu RY , Rehman M , Lee WM . Heat shock protein hsp70 protects cells
from thermal stress even after deletion of its ATP-binding domain.Proc Natl
Acad Sci USA 1992, 89: 2036–2040.
Li Z. Priming of T cells by heat shock protein-peptide complexes as the basis of
tumor vaccines. Semin. Immunol. 1997, 9:315-322
100
曲凌云 参 考 文 献 博士学位论文
Liao XC , Colot HV , Wang Y , Rosbash M : Requirements for U2 snRNP addition to
yeast pre-mRNA.Nucleic Acids Res 1992, 20: 4237–4245.
Liberek K, Skowyra D, Zylicz M, et al. The Escherichia coli DnaK chaperone, the
70-kDa heat shock protein eukaryotic equivalent, changes conformation upon
ATP hydrolysis, thus triggering its dissociation from a bound target protein.
J. Biol. Chem., 1991,266: 14491–14496.
Lima K M, Santos RR, Brandao IT et al, Efficacy of DNA–hsp65 vaccination for
tuberculosis varies with method of DNA introduction in vivo.Vaccine. 2003,
22 (1)：49-56
Lin R, Newman AJ, Cheng S, Abelson J.Yeast mRNA splicing in vitro.J Biol Chem
1985, 260: 14780–14792.
Lindquist S, Craig E A. The heat -shock proteins. Ann Rev Genet, 1988, 22: 631～677
Lindquist S. Translational efficiency of heat-induced messages in Drosophila
melanogaster cells. J Mol Biol. 1980 ,137(2):151-158.
Lindquist S. Varying patterns of protein synthesis in Drosophila during heat shock:
implications for regulation. Dev Biol. 1980，77(2):463-479.
Linquist S. The heat shock response. Annu Rev Chem，1986, 55:1151－1191.
Lithgow T. Targeting of proteins to mitochondria. FEBS Lett., 2000,476: 22–26.
Locke M, Tanguay R M. Increased HSF activation in muscles with a high constitutive
HSP70 expression . Cell Stress Chaperones,1996,1(3):189－195.
Lukacs K V, Nakakes C J, et al.. In vivo gene therapy of malignant tumors with heat
shock protein-65 gene. Gene Ther. 1997, 4:346-353
Lukacs K V, D B Lowrie R W, Stokes M J. Colston.. Tumor cells transfected with a
bacterial heat-shock gene lose tumorigenicity and induce protection against
tumors. J. Exp. Med. 1993, 178:343-349
Lyons C, Dowling V, Tedengren M, et al.Variability of heat shock proteins and
glutathione S-transferase in gill and digestive gland of blue mussel, Mytilus
edulis.Mar Environ Res. 2003,56(5):585-597.
Maass G W, Schmidt M, Berger F, et al. Priming of tumor specific T cells in the
draining lymph nodes after immunization with interleukin 2-secreting tumor
cells: three consecutive stages may be required for successful tumor
vaccination. Proc. Natl. Acad. Sci. USA 1995, 92:5540-5549
Manjili M.H., Wang X.Y., Park J et al. Immunotherapy of cancer using heat shock
proteins. Front Biosci. 2002, 7：43-52
Marber MS, Mestril R, Chi SH. Overexpression of the rat inducible 70-KD heat stress
protein in a transgenic mouse increases the resistance of the heart to ischemic
injury. J. Clin. Invest. 1995,95(4):1446-1453
Margulis BA, Antropova OYu, Kharazova AD.70 kDa heat shock proteins from
mollusc and human cells have common structural and functional
domains.Comp Biochem Physiol B. 1989;94(4):621-623.
101
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Martin J, Mahlke K and Pfanner N. Role of an energized inner membrane in
mitochondrial protein import: drives the movement of presequences. J. Biol.
Chem., 1991, 266, 18051–18057.
Mason PJ, Hall LM and Gausz J. The expression of heat shock genes during normal
development in Drosophila melanogaster. Mol. Gen. Genet., 1984,194: 73-78
Matlack KE, Misselwitz B, Plath K et al. BiP acts as a molecular ratchet during
posttranslational transport of prepro- factor across the ER membrane. Cell,
1999, 97:553–564
Matouschek A, Azem A, Ratliff K, et al. Active unfolding of precursor proteins
during mitochondrial protein import. EMBO J., 1997,16: 6727–6736
Mattingly J R Jr, Iriarte A, et al. Structural features which control folding of
homologous proteins in cell-free translation systems. The effect of a
mitochondrial-targeting presequence on aspartate aminotransferase. J. Biol.
Chem., 1993, 268:26320–26327
Matzinger P. Tolerance, danger and the extended family. Annu. Rev. Immunol. 1994,
12:991998
Matzinger P. An innate sense of danger. Semin. Immunol. 1998,10:399-403
Mehlen P, Schulze-Osthoff K, Arrigo AP. Small stress proteins as novel regulators of
apoptosis, Heat shock protein 27 blocks Fas/APO-1- and
staurosporine-induced cell death. J Biol Chem., 1996, 271(28):16510-16514
Melcher A A S, Todryk N, Hardwick M, Ford M, Jacobson R G Vile. Tumor
immunogenicity is determined by the mechanism of cell death via induction of
heat shock protein expression. Nat. Med. 1998, 4:58-590.
Merlin A, Voos W, Maarse A C, et al.The J-related segment of Tim44 is essential for
cell viability: a mutant Tim44 remains in the mitochondrial import site, but
inefficiently recruits mtHsp70 and impairs protein translocation. J. Cell Biol.,
1999,145:961–972.
Michaud S, Marin R, Tanguay RM.. Regulation of heat shock gene induction and
expression during Drosophila development. Cell Mol Life Sci., 1997，53(1)：
104-113
Mihara K and Omura T. Cytoplasmic chaperones in precursor targeting to
mitochondria: the role of MSF and hsp70. Trends Cell Biol., 1996, 6:104–108.
Mitchell D A, S K Nair E, Gilboa. Dendritic cell/macrophage precursors capture
exogenous antigen for MHC class I presentation by dendritic cells. Eur. J.
Immunol. 1998,28:1923-1928
Morales A V，Haduiargyrou M，et al. Heat shock protein in retinal neurogenesis:
identification of the pMI antigen in comparison to that of other chaperones.
Eur J Neurosci, 1998, 10(10): 3237-3245
Morange M, Diu A, Bensaude O, Babinet C. Altered Expression of Heat Shock
Proteins in Embryonal Carcinoma and Mouse Early Embryonic Cells. Mol.
Cell. Biol., 1984,4:730-735
102
曲凌云 参 考 文 献 博士学位论文
Morimoto R L，Tissieres A. Stress proteins in biology and medicine. Cold Spring
Harbor: CSHL Press, 1990: 1-36
Moro F, Sirrenberg C, Schneider H C, Neupert W and Brunner, M. The TIM17·23
preprotein translocase of mitochondria: composition and function in protein
transport into the matrix. EMBO J., 1999,18, 3667–3675.
Morris J C. Enzyme/Prodrug-Based Tumor Vaccination: All Politics (and Immunity)
Are Local. J Natl Cancer Inst 1999,91: 1986-1989
Muhich ML, Boothroyd JC. Polycistronic transcripts in trypanosomes and their
accumulation during heat shock: evidence for a precursor role in mRNA
synthesis. Mol Cell Biol 1988, 8: 3837–3846.
Mullen C A M M, Coale R, Lowe R M, Blaese. Tumors expressing the cytosine
deaminase suicide gene can be eliminated in vivo with 5-fluorocytosine and
induce protective immunity to wild type tumor. Cancer Res.
1994,54:1503-1509
Multhoff G，Botzler C，Issels R. The role of heat shock protein in the stimulation of an
immune response. Biol Chem., 1998, 379(3): 295-301
Multhoff G C, Botzler L, Jennen J, Schmidt J, Ellwart R Issels. Heat shock protein 72
on tumor cells: a recognition structure for natural killer cells. J. Immunol.
1997,158:4341-4352
Nabel G J A, Chang E G, Nabel G, Plautz B A, Fox L, Huang S Shu. Clinical protocol:
immunotherapy of malignancy by in vivo gene transfer into tumors. Hum.
Gene Ther. 1992,3:399-405
Nakai A, Tanabe M, Kawazoe Y, et al. HSF4, a new member of the human heat shock
factor family which lacks properties of a transcriptional activator. Mol Cell
Biol, 1997, 17(1): 469－481.
Nakanoma T, Ueno M, Iida M, et al Effects of quercetin on the heat-induced
cytotoxicity of prostate cancer cells. Int J Urol. 2001 , 8(11):623-630.
Nathan D F, Lindquist S. Mutational analysis of Hsp90 function: Interactions with a
steroid receptor and protein kinase. Mol Cell Biol, 1995, 15: 3917～3925
Neira J L and Fersht A R. Acquisition of native-like interactions in C-terminal
fragments of barnase. J. Mol. Biol., 1999,287:421–432.
Netzer W and Hartl F. Recombination of protein domains facilitated by
co-translational folding in eukaryotes. Nature, 1997 ,388:343–349.
Neumann S, Ziv E, Lantner F, Schechter I.Regulation of HSP70 gene expression
during the life cycle of the parasitic helminth Schistosoma mansoni.Eur J
Biochem. 1993 ,212(2):589-596.
Neupert W. Protein import into mitochondria. Annu. Rev. Biochem., 1997,66:
863–917
Neupert W, Hartl F U, Craig E A and Pfanner N. How do polypeptides cross the
mitochondrial membranes? Cell, 1990, 63: 447–450.
103
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Newton EM, Knauf U, Green M, et al. The regulatory domain of human heat shock
factor 1 is sufficient to sense heat stress. Mol Cell Biol,1996,16(3):839－846.
Jurivich D, Sistonen L, Kroes R, et al. Effect of sodium sali-cylate on the human heat
shock response. Science,1992, 255(5 049):1 243－1245
Ng R, Abelson J. Isolation and sequence of the gene for actin in Saccharomyces
cerevisiae.Proc Natl Acad Sci USA 1980, 77: 3912–3916.
Ng D T, Brown J D and Walter P. Signal sequences specify the targeting route to the
endoplasmic reticulum membrane. J. Cell Biol., 1996,134:269–278.
Niedzwiecki A, Reveillaud I, Fleming JE. Changes in superoxide dismutasc and
catalase in aging heat-shocked Drosophila. Free Radic Res Commun, 1992,
17(6):355-361
Ochoa GH, Clark YM, Matsumoto B et al. Heat shock protein 70 and heat shock
protein 90 expression in light- and dark-adapted adult octopus retinas. J
Neurocytol. 2002 ,31(2):161-174.
Ohtsuka K.,Suzuki T. Roles of molecular chaperones in the nervous system. Brain
Research Bulletin, 2000, 53(2):141-146
Onozuka M, Watanabe K.Intracellularly applied anti-P70 antibody blocks the
induction of abnormal membrane properties by pentylenetetrazole in identified
Euhadra neurons.Brain Res. 1996 ,716(1):187-191.
Ouellette MM ， Mcdaniel LD ， Wright WE et al. The establishment of
telomerase-immortalized cell lines representing human chromosome
instability syndromes. Hum Mol Gene,2000, 9:403-411
Pardoll D M. Paracrine cytokine adjuvants in cancer immunotherapy. Annu. Rev.
Immunol. 1995,13:399-407
Parsell DA , Kowal AS , Lindquist SL. Saccharomyces cerevisiae Hsp104 protein.J
Biol Chem 1994, 269: 4480–4487
Parsell DA , Kowal AS , Singer M , Lindquist SL. Hsp104-mediated protein
disaggregation: a novel heat-shock protein function. Nature 1994, 372:
475–478.
Parsell DA , Lindquist SL. The function of heat-shock proteins in stress tolerance:
degradation and reactivation of damaged proteins. Annu Rev Genet 1993, 27:
437–496
Parsell DA , Sanchez Y , Stitzel JD , Lindquist S .Hsp104 is a highly conserved
protein with two essential nucleotide-binding sites. Nature 1991, 353:
270–273
Parsell DA, Taulien J, Lindquist S. The role of heat-shock proteins in
thermotolerance.Philos Trans R Soc Lond .1993, 339: 279–285
Paunola E, Suntio T, Jamsa E and Makarow M. Folding of active ?-lactamase in the
yeast cytoplasm before translocation into the endoplasmic reticulum. Mol. Biol.
Cell, 1998,9, 817–827
104
曲凌云 参 考 文 献 博士学位论文
Pelham H R B. A regulatory upstream promoter element in the Drosophila hsp70 heat-
shock gene . Cell，1986,46(5):959－961.
Pfanner N and Meijer M. Pulling in the proteins. Curr. Biol., 1995,5:132–135.
Pfanner N, Craig E A and H?nlinger A. Mitochondrial preprotein translocase. Annu.
Rev. Cell. Dev. Biol., 1997,13, 25–51
Piano A, Asirelli C, Caselli F et al. Hsp70 expression in thermally stressed Ostrea
edulis, a commercially important oyster in Europe. Cell Stress Chaperones.
2002, 7(3)：250-257
Polla BS，Kantengwa S，Franccis D，et al. Mitochondria are selective targets for the
protective effects of heat shock against oxidative injury. Proc Natl Acad Sci
USA,1996,93(13):6458-6463
Porte C, Biosca X, Sole M, Albaiges J. The integrated use of chemical analysis,
cytochrome P450 and stress proteins in mussels to assess pollution along the
Galician coast (NW Spain).Environ Pollut. 2001,112(2):261-268.
Radlowska M, Pempkowiak J.Stress-70 as indicator of heavy metals accumulation in
blue mussel Mytilus edulis. Environ Int. 2002, 27(8):605-608.
Rajasekar R G K, Sim A, Augustin. Self heat shock and T-cell reactivity. Proc. Natl.
Acad. Sci. USA 1990,87:1767-1773
Ramage JM, Young JL, Goodall JC, Gaston JS.T cell responses to heat-shock protein
60: differential responses by CD4+ T cell subsets according to their expression
of CD45 isotypes.J Immunol. 1999,162(2):704-710.
Rassow J, Guiard B, Wienhues U et al. Translocation arrest by reversible folding of a
precursor protein imported into mitochondria. A means to quantitate
translocation contact sites. J. Cell Biol., 1989,109:1421–1428.
Rassow J, Hartl F-U, Guiard B, et al. Polypeptides traverse the mitochondrial
envelope in an extended state. FEBS Lett., 1990, 275:190–194.
Rathinam AV, Chen TT, Grossfeld RM.Cloning and sequence analysis of a cDNA for
an inducible 70 kDa heat shock protein (Hsp70) of the American oyster
(Crassostrea virginica).DNA Seq. 2000,11:261-264.
Reubel GH, Barlough JE, Madigan JE.Production and characterization of Ehrlichia
risticii, the agent of Potomac horse fever, from snails (Pleuroceridae: Juga spp.)
in aquarium culture and genetic comparison to equine strains.J Clin Microbiol.
1998,36(6):1501-1511.
Richard I. Morimoto. Cells in Stress: Transcriptional Activation of Heat Shock Genes.
Science, 1993, 259, (5): 1409-1410
Ritossa F. A new puffing pattern induced by temperature shock and DNP in
Drosophila. Experientia， 1962,18:571－ 573.
Rodolfo M C, Zilocchi C, Melani B, Cappetti I, Arioli G, Parmiani M P.
Immunotherapy of experimental metastases by vaccination with interleukin
gene-transduced adenocarcinoma cells sharing tumor-associated antigens:
105
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
comparison between IL-12 and IL-2 gene-transduced tumor cell vaccines. J.
Immunol. 1996,157:5536-5541
Roy H，Bur Don. Heat shock and the heat shock proteins. Biochem J, 1986, 240：
313-324
Ryan AJ，Flanagan SW，Moseley PL.et al. Acute heat stress protects rates against
endotoxin shock. J Appl Physiol,1992,73(4):1517-1524
S^rensen J G, Loeschcke V. Decreased heat-shock resistance and own-regulation of
Hsp70 expression with increasing age in adult Drosophila melanogaster.
Funct .Ecol. 2002, 16:379–384.
S^rensen, J.G., Loeschcke, V.,. Larval crowding in Drosophila melanogaster induces
Hsp70 expression, and leads to increased adult longevity and adult thermal
stress resistance. J. Insect Physiol. 2001,47:1301–1307.
Samali A, Cotter T G. Heat Shock Proteins Increase Resistance to Apoptosis.
Experimental Cell Research, 1996, 223:163–170
Sanatacruz H, Vriz S, Angelier N. Molecular characterization of a heat shock cognate
cDNA of zebrafish, hsc70, and developmental expression of the corresponding
transcripts. Dev Genetics1997, 21: 223–233.
Sanchez Y, Lindquist SL. HSP104 required for induced thermotolerance. Science
1990, 248: 1112–1115.
Sanchez Y, Parsell DA, Taulien J, et al. Genetic evidence for a functional relationship
between Hsp104 and Hsp70. J Bacteriol 1993, 175: 6484–6491
Sanchez Y, Taulien J , Borkovich KA , Lindquist SL. Hsp104 is required for tolerance
to many forms of stress. EMBO J 1992, 11: 2357–2364
Sanchez Y, Lindquist S.L. HSP104 required for induced thermotolerance. Science,
1990, 248: 1112-1115
Sanders BM, Martin LS, Howe SR, et al. Tissue-specific differences in accumulation
of stress proteins in Mytilus edulis exposed to a range of copper
concentrations.Toxicol Appl Pharmacol. 1994 ,125(2):206-213.
Sanders BM, Martin LS.Stress proteins as biomarkers of contaminant exposure in
archived environmental samples. Sci Total Environ. 1993 ,139:459-470.
Sauter B, Albert M L, Francisco L et al. Consequences of Cell Death: Exposure to
Necrotic Tumor Cells, but Not Primary Tissue Cells or Apoptotic Cells,
Induces the Maturation of Immunostimulatory Dendritic Cells. J. Exp. Med.
2000,191: 423-434
Schatz G and Dobberstein B. Common principles of protein translocation across
membranes. Science, 1996, 271:1519–1526.
Schena M, Picard D, Yamamoto K R.Vectors for constitutive and inducible gene
expression in yeast. In Guide to Yeast Genetics and Molecular Biology. Edited
by Guthrie C, Fink GR. Academic Press, Inc., 1991, 194: 389–398.
Schill RO, Gayle PM, Kohler HR. Daily stress protein (hsp70) cycle in chitons
106
曲凌云 参 考 文 献 博士学位论文
(Acanthopleura granulata Gmelin, 1791) which inhabit the rocky intertidal
shoreline in a tropical ecosystem.Comp Biochem Physiol C Toxicol Pharmacol.
2002 ,131(3):253-258.
Schroder HT, Langer T , Hartl F-U , Bukau B. DnaK, DnaJ, GrpE form a cellular
chaperone machinery capable of repairing heat-induced protein damage.
EMBO J 1993, 12: 4137–4144.
Schwartz M P and Matouschek A. The dimensions of the protein import channels in
the outer and inner mitochondrial membranes. Proc. Natl Acad. Sci. USA,
1999, 96, 13086–13090
Schwartz M P, Huang S and Matouschek A. The structure of precursor proteins
during import into mitochondria. J. Biol. Chem., 1999,274:12759–12764
Schwarz E, Seytter T, Guiard B, et al. Targeting of cytochrome b2 into the
mitochondrial intermembrane space: specific recognition of the sorting signal.
EMBO J., 1993,12: 2295–2302
Shaner SL , Piatt DM , Wensley CG ,et al. Aggregation equilibria of Escherichia coli
RNA polymerase: evidence for anion-linked conformational transitions in the
protomers of core and holoenzyme. Biochemistry 1982, 21: 5539–5551
Shen Z G, Reznikoff G, Dranoff et al. Cloned dendritic cells can present exogenous
antigens on both class I and class II molecules. J. Immunol. 1997,
158:2723-2729
Shi L, Kataoka M and Fink A L. Conformational characterization of DnaK and its
complexes by small-angle X-ray scattering. Biochemistry, 1996, 35:
3297–3308.
Shukla RR , Dominski Z , Zwierzynski T et al. Inactivation of splicing factors in
HeLa cells subjected to heat shock.J Biol Chem 1990, 265: 20377–20383
Simon S M, Peskin C S and Oster G F. What drives the translocation of proteins?
Proc. Natl Acad. Sci. USA, 1992,89:3770–3774.
Sistonen L, Sarge K D, Phillips B, et al. Activation of heat shock factor 2 during
hemin- induced differentiation of human ery-throleukemia cells. Mol Cell Biol,
1992, 12（9）: 4104－4111
Skowyra D, Georgopoulos C , Zylicz M : The E. Coli dnaK gene product, the hsp70
homolog, can reactivate heat-inactivated RNA polymerase in an ATP
hydrolysis-dependent manner. Cell 1990, 62: 939–944
Snyder MJ, Girvetz E, Mulder EP. Induction of marine mollusc stress proteins by
chemical or physical stress.Arch Environ Contam Toxicol. 2001,41(1):22-29.
Sol M, Morcillo Y, Porte C.Stress-protein response in tributyltin-exposed clams. Bull
Environ Contam Toxicol. 2000,64(6):852-858.
Solomon JM, Rossi JM , Golic K et al. Changes in hsp70 alter thermotolerance and
heat-shock regulation in Drosophila. New Biol 1991, 3: 1106–1120
Sorger P K and Pelham H R B. Yeast heat shock factor is an essential DNA- binding
protein that exhibits temperature-dependent phosphorylation. Cell, 1988, 54(6):
107
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
855－864.
Squires C, Squires CL. The CIp proteins: proteolysis regulators or molecular
chaperones. J Bacteriol 1992, 174: 1081–1085
Srivastava PK ， Udono H. Heat shock protein-peptide complexes in cancer
immunotherapy. Curr Opin Immunol. 1994, 6(5): 728-734
Srivastava P K A, Menoret S, Basu R J, Binder K L. McQuade. Heat shock proteins
come of age: primitive functions acquire new roles in an adaptive world.
Immunity 1998,8:657-665
Srivastava P K, Udono H. Heat shock protein-peptide complexes in cancer
immunotherapy. Curr. Opin. Immunol. 1994,6:728-734
Srivastava P H, Udono N E, Blachere Z Li. Heat shock proteins transfer peptides
during antigen processing and CTL priming. Immunogenetics 1994, 39:93-101
Stone DE, Craig EA. Self-regulation of 70-kilodalton heat shock proteins in
Saccharomyces cerevisiae. Mol Cell Biol 1990, 10: 1622–1632.
Sullivan CS，Cantalupo P，Pipas JM. The molecular chaperone activity of simian virus
40 large antigen is required to disrupt Rb-E2F family complexes by an
ATP-dependent mechanism. Mol Cell Biol, 2000,20:6233-6243
Suto R P, Srivastava K. A mechanism for the specific immunogenicity of heat shock
protein-chaperoned peptides. Science 1995,269:1585-1599
Suzue K X, Zhou H N, Eisen R A. Young. Heat shock fusion proteins as vehicles for
antigen delivery into the major histocompatibility complex class I presentation
pathway. Proc. Natl. Acad. Sci. USA 1997, 94:13146-13154
Suzuki H, Kalair W, Shivji G M, Wang B, Toto P, Amerio P, Kraemer K H, Sauder D
N. Impaired Ultraviolet-B-Induced Cytokine Induction in Xeroderma
Pigmentosum Fibroblasts. J Invest Dermatol 2001,117: 1151-1155
Tamura Y P, Peng K, Liu M, Daou P K. Srivastava. Immunotherapy of tumors with
autologous tumor-derived heat shock protein preparations. Science
1997,278:117-129
Tanaka Y, Kobayashi K, Kita M, Kinoshita S, Imanishi J .Messenger RNA expression
of heat shock proteins (HSPs) during ocular development. Curr Eye Res. 1995
14(12):1125-1133.
Tang WB, Ma PQ, Ni H, Zeng WT, Zhu KL. Expression of HSP70 and P-glycoprotein
(P-gp) in human hepatocarcinoma HepG2 cells induced by heat shock and
inhibiting effect of quercetin on them. Ai Zheng. 2003 ,22(9):954-958.
Tatar M. Evolution of senescence: longevity and the expression of heat shock proteins.
Am. Zool. 1999，39：920–927.
Thompson EM, Legouy EL, Christians E, Renard JP. Expression of the HSP70.1 gene,
a landmark of early zygotic activity in the mouse embryo, is restricted to the
first burst of transcription. Development .1995,121:113-122
Tomanek L, Sanford E.Heat-shock protein 70 (Hsp70) as a biochemical stress
108
曲凌云 参 考 文 献 博士学位论文
indicator: an experimental field test in two congeneric intertidal gastropods
(genus: Tegula).Biol Bull. 2003 ,205(3):276-284.
Tomanek L, Somero GN.Interspecific- and acclimation-induced variation in levels of
heat-shock proteins 70 (hsp70) and 90 (hsp90) and heat-shock transcription
factor-1 (HSF1) in congeneric marine snails (genus Tegula): implications for
regulation of hsp gene expression.J Exp Biol. 2002 ,205(5):677-685.
Tomanek L, Somero GN.Time course and magnitude of synthesis of heat-shock
proteins in congeneric marine snails (Genus tegula) from different tidal
heights.Physiol Biochem Zool. 2000 ,73(2):249-256.
Tower J. Transgenic methods for increasing Drosophila life span. Mech Ageing Dev.
2000,118:1-14
Tsdengen M, Olsson B, Reimer J F, et al. Heat pre-treatment increases cadmium
resistance and HSP70 levels in Baltic sea mussels. Aquat. Toxicol.,
2000,48:1-12
Tytell M, Greenberg SG, Lasek RJ.Heat shock-like protein is transferred from glia to
axon. Brain Res. 1986, 363(1):161-164.
Udono H, Saito T, Ogawa M, et al. Hsp-antigen fusion and their use for immunization.
Methods, 2004，32：21-24
Udono H D L, Levey P K Srivastava. Cellular requirements for tumor-specific
immunity elicited by heat shock proteins: tumor rejection antigen gp96 primes
CD8+ T cells in vivo. Proc. Natl. Acad. Sci. USA 1994, 91:3077-3083
Ungermann C, Neupert W and Cyr D M. The role of hsp70 in conferring
unidirectionality on protein translocation into mitochondria. Science, 1994,
266, 1250–1253.
Utans U, Behrens S , Luhrmann R , Kole R , Kramer A. A splicing factor that is
inactivated during in vivo heat shock is functionally equivalent to the
(U4/U6.U5) snRNP-specific proteins.Genes Dev, 1992, 6: 631–641
Vayssier M, Vayssier F, Leguerhier J F, et al. Cloning and analysis of a Trichinella briotovi gene
encoding a cytoplasmic heat shock protein of 72 kDa. Parasitology ,1999, 119:81–93.
Veldhuizen-Tsoerkan MB, Holwerda DA et al. A field study on stress indices in the
sea mussel, Mytilus edulis: application of the "stress approach" in
biomonitoring. Arch Environ Contam Toxicol. 1991, 21(4):497-504.
Veldhuizen-Tsoerkan MB, Holwerda DA,et al. Synthesis of stress proteins under
normal and heat shock conditions in gill tissue of sea mussels (Mytilus edulis)
after chronic exposure to cadmium. Comp Biochem Physiol C.
1991,100(3):699-706.
Vestweber D and Schatz G. DNA–protein conjugates can enter mitochondria via the
protein import pathway. Nature, 1989, 338, 170–172.
Vijayraghavan U, Company M, Abelson J. Isolation and characterization of
pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev 1989, 3:
1206–1216
109
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
Vile R G, Chong H. Immunotherapy: combinatorial molecular immunotherapy: a
synthesis and suggestions. Cancer Metastasis Rev. 1996,15:351-359
Vile R G, Hart I R. In vitro and in vivo targeting of gene expression to melanoma
cells. Cancer Res. 1993,53:962-970
Vile R G, Nelson J A, Castleden S, Chong H, Hart I R. Systemic gene therapy of
murine melanoma using tissue specific expression of the HSVtk gene involves
an immune component. Cancer Res. 1994,54:6228-6233
Vile R G, Castleden S C, Marshall J, Camplejohn R, Upton C, Chong H. Generation
of an anti-tumor immune response in a non-immunogenic tumor:
HSVtk-killing in vivo stimulates a mononuclear cell infiltrate and a Th1-like
profile of intratumoral cytokine expression. Int. J. Cancer 1997,71:267-274
Virca GD, Northemann W , Shiels BR et al. Simplified northern blot hybridization
using 5 % sodium dodecyl sulfate. Biotechniques 1990, 8: 370–371
Voisine C, Craig EA, Zufall N, et al. The protein import motor of mitochondria:
unfolding and trapping of preproteins are distinct and separable functions of
matrix Hsp70. Cell, 1999,97:565–574.
Von Ahsen O, Voos W, Henninger H and Pfanner N. The mitochondrial protein
import machinery: role of ATP in dissociation of the Hsp70–Mim44 complex.
J. Biol. Chem., 1995,270, 29848–29853
Voos W, Gambill B D, Guiard B et al. Presequence and mature part of preproteins
strongly influence the dependence of mitochondrial protein import on heat
shock protein 70 in the matrix. J. Cell Biol., 1993, 123, 119–126
Voos W, Martin H, Krimmer T and Pfanner N. Mechanisms of protein translocation
into mitochondria. Biochim. Biophys. Acta, 1999, 1422: 235–254
Voos W, von Ahsen O, Müller H, et al. Differential requirement for the mitochondrial
Hsp70–Tim44 complex in unfolding and translocation of preproteins. EMBO
J., 1996,15:2668–2677
Wachter C, Schatz G and Glick B S. Protein import into mitochondria: the
requirement for external ATP is precursor-specific whereas intramitochondrial
ATP is universally needed for translocation into the matrix. Mol. Biol. Cell,
1994, 5:465–474
Wadhwa R，Yaguchi T. HSP70 family member, mot-2/mthsp70/grp75, hinds to the
cytoplasmic sequestration domain of the P53 Protein. Cell Res, 2002, 274:
246-253
Walker D, Chaddock A M, Chaddock J A, et al. Ricin A chain fused to a
chloroplast-targeting signal is unfolded on the chloroplast surface prior to
import across the envelope membranes. J. Biol. Chem., 1996 ,271: 4082–4085
Wang F, Li B, Yang B, Yu F. Effects of heat shock protein 70 overexpression on
apoptosis of K562 cell caused by hyperthermia. Wei Sheng Yan Jiu. 2001
30(2):65-67.
Wang X Y, Manjili M H and Chen X, et al. Development of cancer vaccines using
autologous and recombinant high molecular weight stress proteins .Methods,
110
曲凌云 参 考 文 献 博士学位论文
2004，32(1)：13-20
Watanabe N, Tsuji N, Kabayashi D, et al. Endogenous tumor necrosis factor functions
as a resistant factor against hyperthermic cytotoxicity in pancreatic carcinoma
cells via enhancement of the heat shock element- binding activity of heat
shock factor 1 .Chemotherapy,1997,43(6):406－414.
Wei Y -q, Zhao X, Kariya Y, Fukata H, Teshigawara K, Uchida A. Induction of
autologous tumor killing by heat treatment of fresh human tumor cells:
involvement of yd T cells and heat shock protein 70. Cancer Res.
1996,56:1104-1112
Wells AD, Malkovsky M. Heat shock proteins, tumor immunogenicity and antigen
presentation: an integrated view. Immunol Today, 2000, 21(3): 129-133
Wells AD, Rai S K, S M, et al. Hsp-72-mediated augmentation of MHC class I
surface expression and endogenous antigen presentation. Int. Immunol. ,1997,
10:609-615
Welte MA, Tetrault JM, Dellavalle RP, Lindquist SL .A new method for manipulating
transgenes: engineering heat tolerance in a complex, multicellular organism.
Curr Biol 1993, 3: 842–853.
Wendling U，Bloemendal A，Van Der Zee R et al. Entirheumatic E. coli. Extract
OM-89 induces T cell response to HSP60 and 70. Int J Immunopharmacol,
1997, 19: 565-575
Werner I, Hinton DE.Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula
amurensis) in northern San Francisco Bay may be linked to natural rather than
anthropogenic stressors. Mar Environ Res. 2000，50(1):379-384.
Werner-Washburne M , Stone DE , Craig EA. Complex interactions among members
of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae.Mol
Cell Biol 1987, 7: 2568–2577.
Wickner S, Gottesman S, Skowyra D, et al. A molecular chaperone, ClpA, functions
like DnaK and DnaJ.Proc Natl Acad Sci USA 1994, 91: 12218–12222.
Wickner S , Hoskins J , McKenney K. Monomerization of RepA dimers by heat shock
proteins activates binding to DNA replication origin.Proc Natl Acad Sci
USA ,1991, 88: 7903–7907.
Wienhues U, Becker K, Schleyer M et al.Protein folding causes an arrest of preprotein
translocation into mitochondria in vivo. J. Cell Biol., 1991,115:1601–1609
Wu C. Activating protein factor binds in vitro to upstream control sequences in heat
shock gene chromatin . Nature，1984,311 (5981):81－84.
Wu WZ, Liu KD, Xie Q, Wu HS. The study on the expression of membrane HSP70
protein in H22 cell and its immunoprotective mechanism against carcinoma. J
Exp Clin Cancer Res. 1999, 18(4):543-548.
Xiao H, Perisic O, Lis JT. Cooperative binding of Drosophila heat shock factor to
arrays of a conserved 5 bp unit. Cell. 1991, 64(3):585-593
Yem A W, Tomasselli A G and Heinrikson R L. The Hsp56 component of steroid
111
曲凌云 三种养殖扇贝热休克蛋白 HSP70 在逆境因子下的表达和相关基因的克隆 博士学位论文
receptor complexes binds to immobilized FK506 and shows homology to
FKBP-12 and FKBP-13.J. Biol. Chem., 1992, 267(5)：2868 -2871
Yoshino TP, Wu XJ, Liu HD. Transfection and heat-inducible expression of molluscan
promoter-luciferase reporter gene constructs in the Biomphalaria glabrata
embryonic snail cell line. Am J Trop Med Hyg. 1998,59(3):414-420.
Yost HJ, Lindquist S . RNA splicing is interrupted by heat shock and is rescued by
heat shock protein synthesis. Cell 1986, 45: 185–193.
Yost HJ, Lindquist S. Translation of unspliced transcripts after heat shock. Science
1988, 242: 1544–1548.
Yost HJ , Lindquist SL. Heat shock proteins affect RNA processing during the heat
shock response of Saccharomyces cerevisiae.Mol Cell Biol 1991, 11:
1062–1068
Yost HJ , Petersen RB , Lindquist SL. RNA metabolism: strategies for regulation in
the heat shock response. Trends Genet 1990, 6: 223–227
Yura T, Nagai H, Mori H. Regulation of the heat－shock response in bacteria. Annu
Rev Microbiol，1993,47:321－350.
Zhang Y, Frejtag W, Dai R. et al. Heat shock factor-4 (HSF-4a) is a repressor of
HSF-1 mediated transcription. J Cell Biochem. 2001, 82(4)：692-703
Zhu X, Zhao X, Burkholder W F, et al. Structural analysis of substrate binding by the
molecular chaperone Dnak. Science, 1996, 272(5268): 1606-1614
Ziemienowicz A , Skowyra D , Zeilstra-Ryalls J , Fayet O , Georgopoulos C , Zylicz
M. Both the Escherichia coli chaperone systems, GroEL/GroES and
Dnak/Dnaj/GrpE, can reactivate heattreated RNA polymerase. J Biol Chem
1993, 268: 25425–25431
Zimmerman J L，Petri W，Meselson M. Accumulation of specific subset of
Drosophila melanogaster mRNA in normal development without heat shock.
Cell, 1983, 32:1161-1170
Zugel U, Kaufmann SH. Activation of CD8 T cells with specificity for mycobacterial heat
shock protein 60 in Mycobacterium bovis bacillus Calmette-Guerin-vaccinated mice.
Infect Immun. 1997,65(9): 3947-3950
Zugel U，Kaufmann SH. Immune response against heat shock protein in infectious
diseases. Innunobiology, 1999, 201(1): 22-27
Zugel U，Kaufmann SH. Role of heat shock proteins in protection from pathogensis of
infectious diseases. Clin Microbiol Rev, 1999, 12(1):19-24
Zylicz M. The Escherichia coli chaperones involved in DNA replication.Philos Trans
R Soc Lond [Biol] 1993, 339: 271–277.