弹性蛋白的层层自组装改性研究
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摘要
基于弹性蛋白良好的性能和层层自组装技术的在生物材料表面改性中的一系列优点,本课题首先以弹性蛋白膜为基底材料,分别以细胞外基质成分透明质酸、Ⅰ型胶原和透明质酸、天然多糖壳聚糖为组装材料通过层层自组装成功的对弹性蛋白膜进行了表面改性;其次,将弹性蛋白部分降解后应用冷冻干燥法制备了弹性蛋白三维支架,并对支架进行了自组装改性探索。
1.成功在弹性蛋白膜表面构筑了透明质酸/胶原多层膜,扫描电镜测试显示,随组装层数增加弹性蛋白膜表面粗糙程度逐渐降低,组装14层后弹性蛋白膜表平整,缺陷基本消失,组装14层的弹性蛋白膜纵切面照片直观证明了基材表面多层膜结构的存在;X光电子能谱测试证实了组装膜为透明质酸/胶原;紫外-可见光谱测试证实了透明质酸/胶原多层膜的线性增长;动态接触角测试表明,组装后弹性蛋白膜表面呈现良好的亲水性,组装14层样品的前进接触角由组装前的121.76°降低到69.33°;拉伸测试显示弹性蛋白拉伸强度在840-870 KPa范围内,断裂伸长率在140-160%范围内,改性对其力学性能影响不大。
2.成功在弹性蛋白膜表面组装了层透明质酸/壳聚糖多层膜,扫描电镜照片显示,随组装层数增加弹性蛋白膜表面粗糙程度逐渐降低,组装14层的弹性蛋白膜的表面平整,表面缺陷显著减少;X光电子能谱测试表明组装膜为透明质酸/壳聚糖;动态接触角测试显示,组装后弹性蛋白膜表面呈现良好的亲水性,组装14层样品的前进接触角由组装前的121.76°降低到80.13°;拉伸测试显示弹性蛋白拉伸强度在840-870 KPa范围内,断裂伸长率在130-160%范围内,改性对其力学性能几乎没有影响。
3.成功制备了性能较好的弹性蛋白三维支架,0.15 mol/L NaOH溶液降解2.5 h后制备的支架的孔径可达100μm且较均匀,孔隙间贯通性较好,孔隙率为76.2%,吸水率为126.3%,拉伸强度可达112.6 KPa,0.3 mol/L草酸溶液降解4 h后制备的支架平均孔径在80-100μm左右,孔隙间贯通性较好,孔隙率为83.7%,吸水率为156.5%,拉伸强度为81.5 KPa。并初步利用1、2中的方法对性能较好的弹性蛋白支架进行了表面改性,改性后支架的表面形貌没有明显的变化、孔径基本不变、孔隙率有所降低、吸水能力明显提高、拉伸强度有所提高。
Based on the good properties of elastin and the series advantages of layer-by-layer self-assembly technology in the surface modification of biomaterials, in this paper, first of all, elastin were modified via layer-by-layer self-assembly of hyaluronic acid(HA)/collagen(typeⅠ) and the hyaluronic acid(HA)/chitosan(CS) respectively. Secondly, elastin was degraded partly and prepared into 3D scaffolds, and the modifi- cation of the elastin scaffolds was also explored.
1. HA/collagen multilayer films were successfully constructed on the elastin membranes. SEM images showed it was clear sandwich structures on an edge of the elastin membranes containing 14 layers of HA and collagen. The XPS analysis demonstrated that the assembly films were HA and collagen. The UV-visible spectroscopy results demonstrated the linearity of the multilayer build-up. The advancing contact angle data revealed the hydrophilicity of the elastin membranes was greatly improved after assembly.The tensile test data showed the mechanical properties of elastin membranes had not changed after the modification.
2. Elastin membranes were successfully modified via layer-by-layer self-assembly of HA and CS. SEM images showed the surface roughness of elastin membranes gradually decreased with the number of the multilayer’s increasing. The XPS analysis demonstrated that the assembly films were HA and CS. The advancing contact angle data revealed the hydrophilicity of the elastin membranes was greatly improved after assembly. The tensile test data showed the mechanical properties of elastin membranes had not changed after the modification.
3. Elastin 3D scaffolds with good properties were successfully prepared. The pore diameter of the scaffolds prepared after 2.5 h degraded in the 0.15mol/L NaOH solution reached 100μm and the pores are absolutely open. The porosity are 76.2% and the tensile strength are 112.6 KPa. The pore diameter of the scaffolds prepared after 4 h degraded in the 0.3 mol/L oxalic acid solution are in the range of 80-100μm and the pores are absolutely open. The porosity are 83.7% and the tensile strength are 81.5 KPa. Furthermore, the elastin scaffolds were modified via layer-by-layer self-assembly of HA/collagen and HA/CS, and the modification improved the bibulous ratio of the scaffolds.
1.成功在弹性蛋白膜表面构筑了透明质酸/胶原多层膜,扫描电镜测试显示,随组装层数增加弹性蛋白膜表面粗糙程度逐渐降低,组装14层后弹性蛋白膜表平整,缺陷基本消失,组装14层的弹性蛋白膜纵切面照片直观证明了基材表面多层膜结构的存在;X光电子能谱测试证实了组装膜为透明质酸/胶原;紫外-可见光谱测试证实了透明质酸/胶原多层膜的线性增长;动态接触角测试表明,组装后弹性蛋白膜表面呈现良好的亲水性,组装14层样品的前进接触角由组装前的121.76°降低到69.33°;拉伸测试显示弹性蛋白拉伸强度在840-870 KPa范围内,断裂伸长率在140-160%范围内,改性对其力学性能影响不大。
2.成功在弹性蛋白膜表面组装了层透明质酸/壳聚糖多层膜,扫描电镜照片显示,随组装层数增加弹性蛋白膜表面粗糙程度逐渐降低,组装14层的弹性蛋白膜的表面平整,表面缺陷显著减少;X光电子能谱测试表明组装膜为透明质酸/壳聚糖;动态接触角测试显示,组装后弹性蛋白膜表面呈现良好的亲水性,组装14层样品的前进接触角由组装前的121.76°降低到80.13°;拉伸测试显示弹性蛋白拉伸强度在840-870 KPa范围内,断裂伸长率在130-160%范围内,改性对其力学性能几乎没有影响。
3.成功制备了性能较好的弹性蛋白三维支架,0.15 mol/L NaOH溶液降解2.5 h后制备的支架的孔径可达100μm且较均匀,孔隙间贯通性较好,孔隙率为76.2%,吸水率为126.3%,拉伸强度可达112.6 KPa,0.3 mol/L草酸溶液降解4 h后制备的支架平均孔径在80-100μm左右,孔隙间贯通性较好,孔隙率为83.7%,吸水率为156.5%,拉伸强度为81.5 KPa。并初步利用1、2中的方法对性能较好的弹性蛋白支架进行了表面改性,改性后支架的表面形貌没有明显的变化、孔径基本不变、孔隙率有所降低、吸水能力明显提高、拉伸强度有所提高。
Based on the good properties of elastin and the series advantages of layer-by-layer self-assembly technology in the surface modification of biomaterials, in this paper, first of all, elastin were modified via layer-by-layer self-assembly of hyaluronic acid(HA)/collagen(typeⅠ) and the hyaluronic acid(HA)/chitosan(CS) respectively. Secondly, elastin was degraded partly and prepared into 3D scaffolds, and the modifi- cation of the elastin scaffolds was also explored.
1. HA/collagen multilayer films were successfully constructed on the elastin membranes. SEM images showed it was clear sandwich structures on an edge of the elastin membranes containing 14 layers of HA and collagen. The XPS analysis demonstrated that the assembly films were HA and collagen. The UV-visible spectroscopy results demonstrated the linearity of the multilayer build-up. The advancing contact angle data revealed the hydrophilicity of the elastin membranes was greatly improved after assembly.The tensile test data showed the mechanical properties of elastin membranes had not changed after the modification.
2. Elastin membranes were successfully modified via layer-by-layer self-assembly of HA and CS. SEM images showed the surface roughness of elastin membranes gradually decreased with the number of the multilayer’s increasing. The XPS analysis demonstrated that the assembly films were HA and CS. The advancing contact angle data revealed the hydrophilicity of the elastin membranes was greatly improved after assembly. The tensile test data showed the mechanical properties of elastin membranes had not changed after the modification.
3. Elastin 3D scaffolds with good properties were successfully prepared. The pore diameter of the scaffolds prepared after 2.5 h degraded in the 0.15mol/L NaOH solution reached 100μm and the pores are absolutely open. The porosity are 76.2% and the tensile strength are 112.6 KPa. The pore diameter of the scaffolds prepared after 4 h degraded in the 0.3 mol/L oxalic acid solution are in the range of 80-100μm and the pores are absolutely open. The porosity are 83.7% and the tensile strength are 81.5 KPa. Furthermore, the elastin scaffolds were modified via layer-by-layer self-assembly of HA/collagen and HA/CS, and the modification improved the bibulous ratio of the scaffolds.
引文
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