镉胁迫对褐飞虱的影响
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摘要
为探讨镉胁迫对植食性昆虫褐飞虱的影响,本文通过设含镉量为0、2、4、6、8、16、32mg/kg的土壤种植水稻,连续饲养褐飞虱F1代和F2代,研究了褐飞虱的若虫存活率、发育历期、成虫长短翅比例和雌成虫寿命;并采用双向电泳技术和质谱鉴定技术研究了镉胁迫对褐飞虱响应蛋白。结果表明:土壤中高浓度的镉会对褐飞虱产生不利的影响,低浓度的影响不明显。
镉胁迫对褐飞虱具有明显的影响,且呈剂量效应。与对照(0mg/kg)比较,镉胁迫浓度为2mg/kg时褐飞虱的若虫存活率、发育历期变化不显著,成虫长短翅比例和雌成虫寿命显著提高,F2代长短翅比例显著低于F1代;镉胁迫浓度为4、6、8mg/kg时褐飞虱若虫存活率和成虫长短翅比例显著降低,发育历期和雌成虫寿命显著缩短,F2代长短翅比例显著低于F1代,但存活率F2代显著高于F1代;镉胁迫浓度为16、32mg/kg时若虫存活率和成虫长短翅比例显著降低,
雌成虫寿命都显著缩短,而发育历期却显著延长,F2代存活率显著高于F1代。镉胁迫对褐飞属响应蛋白研究表明,镉胁迫引起褐飞虱蛋白质组的变化。土壤中的镉通过水稻进入褐飞虱体内,有多种类型的响应蛋白,包括:细胞氧化损伤的蛋白,DNA损伤有关蛋白,细胞通透性相关蛋白,细胞结构相关蛋白,代谢相关蛋白,信号转导蛋白,细胞分裂相关蛋白,蛋白质生成、降解及转移的相关蛋白等等。其中有些蛋白是对细胞产生毒害作用,另一些是起解毒作用的蛋白。在高浓度(4、6、8、16mg/kg)镉胁迫下,毒害作用占主导;在低浓度(2mg/kg)镉胁迫下,机体自身有很好的解毒功能,毒害作用不明显。
This experiment was conducted to study the effects of different concentrations of cadmium stress in soil on larval survival, larval duration, wing dimorphism, female adult longevity and different proteins of two generations (F1 and F2) of the rice brown plant hopper (BPH), Nilaparvata lugens St?l. The results were as follows:
Cadmium stress influenced the BPH, and had a dose-response. Compared with the control (0 mg/kg), there was no significant difference in larval survival or larval duration when the cadmium concentration was 2 mg/kg, but the ratio of macropterous forms to brachypterous forms and female adult longevity were increased significantly. Also, the ratio of macropterous forms to brachypterous forms of the F2 generation was significantly lower than that of the F1 generation. Larval survival, larval duration, the ratio of macropterous forms to brachypterous forms and female adult longevity were significantly reduced at cadmium concentrations of 4, 6 and 8 mg/kg, and similarly to the cadmium concentration of 2 mg/kg, the ratio of macropterous forms to brachypterous forms of the F2 generation was significantly lower than that of the F1 generation. When the cadmium concentration was 16 or 32 mg/kg, except for the larval duration lengthening, all of the other variables measured were reduced for the F1 generation and the F2 generation compared to the control. However, the larval survival of the F2 generation was higher than that of the F1 generation.
Cadmium stress changed the proteome of female adult BPHs. There were various types of responsive proteins, which relate to hydrogen peroxide-induced injury in cells, DNA damage or repair, membrane permeability in cells, cellular structure, metabolism, signal transduction, cell division, protein synthesis, protein degradation and protein transformation. Some of the proteins poison cells, and others are for detoxification. At the concentrations of 4, 6 and 8 and 16 mg/kg of cadmium stress, toxicity was dominant. At the low concentration (2mg/kg) of cadmium stress, the organism itself had very good alexipharmic function, and toxicity was not obvious.
In essence, high concentrations of cadmium in soil negatively affect the brown plant hopper, but at low concentrations of cadmium, the effects are not significant.
镉胁迫对褐飞虱具有明显的影响,且呈剂量效应。与对照(0mg/kg)比较,镉胁迫浓度为2mg/kg时褐飞虱的若虫存活率、发育历期变化不显著,成虫长短翅比例和雌成虫寿命显著提高,F2代长短翅比例显著低于F1代;镉胁迫浓度为4、6、8mg/kg时褐飞虱若虫存活率和成虫长短翅比例显著降低,发育历期和雌成虫寿命显著缩短,F2代长短翅比例显著低于F1代,但存活率F2代显著高于F1代;镉胁迫浓度为16、32mg/kg时若虫存活率和成虫长短翅比例显著降低,
雌成虫寿命都显著缩短,而发育历期却显著延长,F2代存活率显著高于F1代。镉胁迫对褐飞属响应蛋白研究表明,镉胁迫引起褐飞虱蛋白质组的变化。土壤中的镉通过水稻进入褐飞虱体内,有多种类型的响应蛋白,包括:细胞氧化损伤的蛋白,DNA损伤有关蛋白,细胞通透性相关蛋白,细胞结构相关蛋白,代谢相关蛋白,信号转导蛋白,细胞分裂相关蛋白,蛋白质生成、降解及转移的相关蛋白等等。其中有些蛋白是对细胞产生毒害作用,另一些是起解毒作用的蛋白。在高浓度(4、6、8、16mg/kg)镉胁迫下,毒害作用占主导;在低浓度(2mg/kg)镉胁迫下,机体自身有很好的解毒功能,毒害作用不明显。
This experiment was conducted to study the effects of different concentrations of cadmium stress in soil on larval survival, larval duration, wing dimorphism, female adult longevity and different proteins of two generations (F1 and F2) of the rice brown plant hopper (BPH), Nilaparvata lugens St?l. The results were as follows:
Cadmium stress influenced the BPH, and had a dose-response. Compared with the control (0 mg/kg), there was no significant difference in larval survival or larval duration when the cadmium concentration was 2 mg/kg, but the ratio of macropterous forms to brachypterous forms and female adult longevity were increased significantly. Also, the ratio of macropterous forms to brachypterous forms of the F2 generation was significantly lower than that of the F1 generation. Larval survival, larval duration, the ratio of macropterous forms to brachypterous forms and female adult longevity were significantly reduced at cadmium concentrations of 4, 6 and 8 mg/kg, and similarly to the cadmium concentration of 2 mg/kg, the ratio of macropterous forms to brachypterous forms of the F2 generation was significantly lower than that of the F1 generation. When the cadmium concentration was 16 or 32 mg/kg, except for the larval duration lengthening, all of the other variables measured were reduced for the F1 generation and the F2 generation compared to the control. However, the larval survival of the F2 generation was higher than that of the F1 generation.
Cadmium stress changed the proteome of female adult BPHs. There were various types of responsive proteins, which relate to hydrogen peroxide-induced injury in cells, DNA damage or repair, membrane permeability in cells, cellular structure, metabolism, signal transduction, cell division, protein synthesis, protein degradation and protein transformation. Some of the proteins poison cells, and others are for detoxification. At the concentrations of 4, 6 and 8 and 16 mg/kg of cadmium stress, toxicity was dominant. At the low concentration (2mg/kg) of cadmium stress, the organism itself had very good alexipharmic function, and toxicity was not obvious.
In essence, high concentrations of cadmium in soil negatively affect the brown plant hopper, but at low concentrations of cadmium, the effects are not significant.
引文
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