鸡传染性法氏囊病病毒主要结构蛋白的克隆与表达
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摘要
传染性法氏囊病(Infectious bursal disease, IBD)是由传染性法氏囊病病毒(Infectious bursal disease virus, IBDV)引起的一种急性、高度接触性传染病,是危害世界养禽业的主要传染病之一,该病主要侵害雏鸡的中枢免疫器官―法氏囊,造成免疫抑制,给养鸡企业带来了巨大的经济损失。
IBD于1957年首次在美国特拉华州甘布罗镇(Gumboro)的肉鸡群中发现,于20世纪80年代在欧洲国家首先报道有超强毒株(vvIBDV)和变异株的出现,于90年代初vvIBDV传至中国及亚洲其它国家和地区。随着变异株、超强毒株的出现,该病的发生与流行不断出现新的特点,给该病的诊断与防治带来了更多的困难,引起了人们的高度关注。
近年来,IBDV的分子生物学研究己取得了较大进展,但在分子水平对鸡传染性法氏囊病病毒的免疫机理、致病机制、病毒分子结构与功能方面仍然有许多问题有待于进一步深入研究,鉴定IBDV主要结构蛋白的功能有助于揭示病毒与机体相互作用的本质,从而了解IBDV的致病机制和免疫机理;表达的主要结构蛋白为进一步制备单克隆抗体,进而研制快速诊断试剂盒以及多表位疫苗打下了基础。
本研究共分三个部分:
试验一免疫鸡群中IBDV山东地方株的分离鉴定
从山东地区采集已免疫过IBDV疫苗,但又表现出IBD临床症状的病鸡病料,根据病鸡发病的流行特点和临床症状,结合病毒分离、组织病理学观察、琼脂免疫扩散试验、分子生物学诊断、动物回归试验、IBDV VP2基因的序列同源性分析等结果,确定鸡场的疫情是由感染IBDV野毒引起,该分离株命名为IBDV SD株。
试验二IBDV SD株主要结构蛋白VP2基因在BL21(DE3)plysS中的表达及免疫血清的制备
IBDV主要结构蛋白VP2基因既是主要保护性蛋白,又与病毒的致病性相关,本研究利用RT-PCR技术扩增出IBDV的结构蛋白VP2基因,并将其克隆到原核表达载体PGEX-4T-3中,获得重组质粒命名为PGEX-VP2,经PCR、酶切和序列分析鉴定表明:插入的位置、大小和读码框均正确。SDS- PAGE电泳检测表明,经重组质粒PGEX-VP2转化、诱导的受体菌E.coli BL21(DE3)plysS能表达结构蛋白VP2基因,约69KDa,表达的蛋白经纯化后免疫6周龄BALB/c小鼠,ELISA分析表明制备的抗血清效价在1:5120以上,Western blot分析表明VP2表达产物能与IBDV抗血清发生反应,具有良好的免疫原性,为单克隆抗体的制备及快速诊断试剂盒的制备奠定了基础。
试验三IBDV SD株主要结构蛋白VP3基因的克隆表达与生物学活性的鉴定
为研究IBDV SD株主要结构蛋白VP3基因与致病性及免疫原性的相关性,本研究利用RT-PCR技术扩增出IBDV的结构蛋白VP3基因,将其克隆到原核表达载体pET32a中,获得重组质粒,命名为pET-VP3,经PCR、酶切和序列分析鉴定表明:插入的位置、大小和读码框均正确。SDS-PAGE检测表明,经重组质粒pET-VP3转化、诱导的受体菌BL21(DE3)能表达结构蛋白VP3基因,并以包涵体形式存在,蛋白约为57KDa,经Western blot等检测表明,诱导表达的蛋白能与IBDV阳性血清发生特异性反应。
通过切胶纯化蛋白,并将纯化的蛋白免疫SPF鸡,然后以点眼滴鼻的形式攻毒,结果表明:注射VP3表达蛋白的鸡能抵御IBDV强毒的攻击,说明IBDV VP3基因不但具有很好的免疫原性,而且还具有一定的免疫保护作用,为血清学诊断和下一步基因工程疫苗的制备提供了依据。
Infectious bursal disease virus(IBDV),the causative agent of infectious bursal disease(IBD),causes immunosuppression in young chickens by destroying the precursors of B lympHocytes in the bursa of Fabricius. And enhanced any other blight susceptibility.In 1957,the disease first broke out in broilers in Gumboro,Telahua state of America.In the 1980s,the variation strain of IBDV was first appeared in America. Acute IBD caused by very virulent infectious bursal disease virus(vvIBDV) was first reported in Europe at the end of 1980s.The acute form of the disease were then transmitted to Japan in the early 1990s,and then rapidly spreaded all over Asia and other major parts of the world.The IBDV has beeen the cause of significant economic losses in poltry industry for a long time.
Recent years,molecular biology research of infectious bursal disease virus achieved great advance,but the study of immune and pathogenic mechanism、molecular structure and function of infectious bursal disease virus need further research.Thus,we had identified the function of the main structural protein of infectious bursal disease virus which is valuable for further research such as the preparation of monoclonal antibody to get a rapid diagnosis kit about this kind of virus which is useful for prevention and cure it.
The research divide three parts:
Part one: Isolation and Identification of Shandong IBDV strain
Collecting pathological tissue from inoculated chickens in Shandong province,according to the epidemiology and clinic symptom of infected chickens, virus isolation,results of tissue pathology,agar gel immunodiffusion test(AGID),molecular biology diagnosis,animal regression test,sequence homology analysis,one IBDV strain had been isolated and identified. and we named it as IBDV SD strain.
Part two: Expression of IBDV Structural Protein VP2 Gene in BL21(DE3)plysS and Preparation of immune serum
IBDV structural protein VP2 gene is protective protein and related with pathogenicity which is foundation of rapid diagnosis kit.By RT-PCR,the structural protein gene VP2 of infectious bursal disease virus (IBDV) was determined from segment A of IBDV.The gene was cloned into expressing vector PGEX-4T-3.The recombinant plasmid named PGEX-VP2 was constructed. The PGEX-VP2 was used to transform into E. coli BL21(DE3) plysS. The results of SDS- PAGE and Western blot indicated that the VP2 protein was expressed in high level which was about 69 KDa , the high titer anti-VP2 serum was also prepared in BALB /c mice immunized with purified VP2 fusion protein inclusion.ELISA results showed that titer was above 1:5120 and Western blot analysis showed that the expressed protein VP2 reacted with polyclonal antibody,It explained that the expressed protein VP2 possess specific satisfactory immunological reaction.had immunological reactive activity.
Part three: Clone Expression and Biologic Activity Identification of VP3 Gene of IBDV Shandong strain
In order to study the relataion to pathogenicity or immunogenic of Shandong strain IBDV VP3 gene,By PCR,a structural protein gene VP3 of infectious bursal disease virus (IBDV) was determined from segment A of IBDV.The gene was cloned into expressing vector PET32a.The recombinant plasmid named PET-VP3 was constructed.The PET-VP3 was used to transform into E.coli BL21(DE3).The results of SDS-PAGE and Western blot indicated that the VP3 protein was expressed in high level and mainly existed as inclusion bodies,which was about 57 KDa,had immunological reactive activity.
Purify the protein and immune chicken with the protein,and then give IBDV to the chicken, the result shows that IBDV VP3 gene can withstand virus.This study lay on foundation for the development of the serological diagnosis and the preparation of genetically engineering vaccine.
IBD于1957年首次在美国特拉华州甘布罗镇(Gumboro)的肉鸡群中发现,于20世纪80年代在欧洲国家首先报道有超强毒株(vvIBDV)和变异株的出现,于90年代初vvIBDV传至中国及亚洲其它国家和地区。随着变异株、超强毒株的出现,该病的发生与流行不断出现新的特点,给该病的诊断与防治带来了更多的困难,引起了人们的高度关注。
近年来,IBDV的分子生物学研究己取得了较大进展,但在分子水平对鸡传染性法氏囊病病毒的免疫机理、致病机制、病毒分子结构与功能方面仍然有许多问题有待于进一步深入研究,鉴定IBDV主要结构蛋白的功能有助于揭示病毒与机体相互作用的本质,从而了解IBDV的致病机制和免疫机理;表达的主要结构蛋白为进一步制备单克隆抗体,进而研制快速诊断试剂盒以及多表位疫苗打下了基础。
本研究共分三个部分:
试验一免疫鸡群中IBDV山东地方株的分离鉴定
从山东地区采集已免疫过IBDV疫苗,但又表现出IBD临床症状的病鸡病料,根据病鸡发病的流行特点和临床症状,结合病毒分离、组织病理学观察、琼脂免疫扩散试验、分子生物学诊断、动物回归试验、IBDV VP2基因的序列同源性分析等结果,确定鸡场的疫情是由感染IBDV野毒引起,该分离株命名为IBDV SD株。
试验二IBDV SD株主要结构蛋白VP2基因在BL21(DE3)plysS中的表达及免疫血清的制备
IBDV主要结构蛋白VP2基因既是主要保护性蛋白,又与病毒的致病性相关,本研究利用RT-PCR技术扩增出IBDV的结构蛋白VP2基因,并将其克隆到原核表达载体PGEX-4T-3中,获得重组质粒命名为PGEX-VP2,经PCR、酶切和序列分析鉴定表明:插入的位置、大小和读码框均正确。SDS- PAGE电泳检测表明,经重组质粒PGEX-VP2转化、诱导的受体菌E.coli BL21(DE3)plysS能表达结构蛋白VP2基因,约69KDa,表达的蛋白经纯化后免疫6周龄BALB/c小鼠,ELISA分析表明制备的抗血清效价在1:5120以上,Western blot分析表明VP2表达产物能与IBDV抗血清发生反应,具有良好的免疫原性,为单克隆抗体的制备及快速诊断试剂盒的制备奠定了基础。
试验三IBDV SD株主要结构蛋白VP3基因的克隆表达与生物学活性的鉴定
为研究IBDV SD株主要结构蛋白VP3基因与致病性及免疫原性的相关性,本研究利用RT-PCR技术扩增出IBDV的结构蛋白VP3基因,将其克隆到原核表达载体pET32a中,获得重组质粒,命名为pET-VP3,经PCR、酶切和序列分析鉴定表明:插入的位置、大小和读码框均正确。SDS-PAGE检测表明,经重组质粒pET-VP3转化、诱导的受体菌BL21(DE3)能表达结构蛋白VP3基因,并以包涵体形式存在,蛋白约为57KDa,经Western blot等检测表明,诱导表达的蛋白能与IBDV阳性血清发生特异性反应。
通过切胶纯化蛋白,并将纯化的蛋白免疫SPF鸡,然后以点眼滴鼻的形式攻毒,结果表明:注射VP3表达蛋白的鸡能抵御IBDV强毒的攻击,说明IBDV VP3基因不但具有很好的免疫原性,而且还具有一定的免疫保护作用,为血清学诊断和下一步基因工程疫苗的制备提供了依据。
Infectious bursal disease virus(IBDV),the causative agent of infectious bursal disease(IBD),causes immunosuppression in young chickens by destroying the precursors of B lympHocytes in the bursa of Fabricius. And enhanced any other blight susceptibility.In 1957,the disease first broke out in broilers in Gumboro,Telahua state of America.In the 1980s,the variation strain of IBDV was first appeared in America. Acute IBD caused by very virulent infectious bursal disease virus(vvIBDV) was first reported in Europe at the end of 1980s.The acute form of the disease were then transmitted to Japan in the early 1990s,and then rapidly spreaded all over Asia and other major parts of the world.The IBDV has beeen the cause of significant economic losses in poltry industry for a long time.
Recent years,molecular biology research of infectious bursal disease virus achieved great advance,but the study of immune and pathogenic mechanism、molecular structure and function of infectious bursal disease virus need further research.Thus,we had identified the function of the main structural protein of infectious bursal disease virus which is valuable for further research such as the preparation of monoclonal antibody to get a rapid diagnosis kit about this kind of virus which is useful for prevention and cure it.
The research divide three parts:
Part one: Isolation and Identification of Shandong IBDV strain
Collecting pathological tissue from inoculated chickens in Shandong province,according to the epidemiology and clinic symptom of infected chickens, virus isolation,results of tissue pathology,agar gel immunodiffusion test(AGID),molecular biology diagnosis,animal regression test,sequence homology analysis,one IBDV strain had been isolated and identified. and we named it as IBDV SD strain.
Part two: Expression of IBDV Structural Protein VP2 Gene in BL21(DE3)plysS and Preparation of immune serum
IBDV structural protein VP2 gene is protective protein and related with pathogenicity which is foundation of rapid diagnosis kit.By RT-PCR,the structural protein gene VP2 of infectious bursal disease virus (IBDV) was determined from segment A of IBDV.The gene was cloned into expressing vector PGEX-4T-3.The recombinant plasmid named PGEX-VP2 was constructed. The PGEX-VP2 was used to transform into E. coli BL21(DE3) plysS. The results of SDS- PAGE and Western blot indicated that the VP2 protein was expressed in high level which was about 69 KDa , the high titer anti-VP2 serum was also prepared in BALB /c mice immunized with purified VP2 fusion protein inclusion.ELISA results showed that titer was above 1:5120 and Western blot analysis showed that the expressed protein VP2 reacted with polyclonal antibody,It explained that the expressed protein VP2 possess specific satisfactory immunological reaction.had immunological reactive activity.
Part three: Clone Expression and Biologic Activity Identification of VP3 Gene of IBDV Shandong strain
In order to study the relataion to pathogenicity or immunogenic of Shandong strain IBDV VP3 gene,By PCR,a structural protein gene VP3 of infectious bursal disease virus (IBDV) was determined from segment A of IBDV.The gene was cloned into expressing vector PET32a.The recombinant plasmid named PET-VP3 was constructed.The PET-VP3 was used to transform into E.coli BL21(DE3).The results of SDS-PAGE and Western blot indicated that the VP3 protein was expressed in high level and mainly existed as inclusion bodies,which was about 57 KDa,had immunological reactive activity.
Purify the protein and immune chicken with the protein,and then give IBDV to the chicken, the result shows that IBDV VP3 gene can withstand virus.This study lay on foundation for the development of the serological diagnosis and the preparation of genetically engineering vaccine.
引文
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