脂肪酶产生菌的筛选及固定化脂肪酶在生产生物柴油中的应用研究
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摘要
生物柴油是由大豆油、菜油等植物油或动物脂肪通过低碳醇在催化剂作用下形成的长链脂肪酸单酯类物质,具有不受地理、环境等因素的影响,可减少对石油市场的依赖等优点而受到越来越多的关注。
原料的价格、供应和廉价的脂肪酶是影响酶法生产生物柴油的关键因素。为解决这些问题,在本研究中,筛选了25株脂肪酶产生菌并对其中效果较好的2个脂肪酶进行催化生产生物柴油的研究,同时研究了原料的影响,为筛选合适的原料提供了参考。
研究内容和结果如下:
1.筛选得到25株脂肪酶菌种,其中8株活性较高,对其中活性较好的3株进行,并种类鉴定、最佳培养条件确定等研究。
产气肠杆菌(Enterobacter aerogenes)以1.0%的葡萄糖和1.5%的NaHCO_3为C源,1.5%的蛋白胨为N源,添加0.1%MgSO_4.7 H_2O,起始pH 9.0,装液量50mL/100 mL,120 rpm,30℃培养48 h,酶活达34.16 U/mL;加入1.0%的橄榄油,酶活可提高到66.31 U/mL。该脂肪酶在30℃,pH 8.0时酶催化活性较高。
成团肠杆菌(Enterobacter agglomerans)以2%的乳糖作为碳源,1.5%的牛肉浸膏和1.0%的酵母膏作为氮源,添加0.1%MgSO_4·7 H_2O,初始pH 7.0,120 r/min,30℃培养48 h时产酶活力达39.09 U/mL。发酵液在30℃,180 r/min时催化性能较强。在反应体系中含水量达91%以上时,仍可催化芝麻油生产生物柴油,产率达54.51%。
乙酸钙不动杆菌(Acinetobacter calcoaceticus),以1.5%的麦芽糖作为碳源,2.5%的NH_4Cl为氮源,初始pH 7.0,接种量1.0%,装液量40 mL/100 mL,120 r/min下30℃培养24 h,酶活达9.07 U/mL。2.确定了气相色谱内标法测定生物柴油方法
以十七酸甲酯为内标,依据菜籽油组分含量不同,改进了气相色谱内标法测定生物柴油方法,建立了棕榈酸甲酯、硬脂酸甲酯、油酸甲酯、亚油酸甲酯、亚麻酸甲酯和花生一烯酸甲酯的线性方程,使各个组分同时处于测量范围内,确保了测定结果的准确性。各脂肪酸甲酯含量在各自线性范围内的相关系数r≥0.999、平均回收率在97.91%-100.45%,精密度高,试验标准偏差在0.28%-2.22%,重复性好。该体系可用于菜籽油合成生物柴油产率测定,也可用于原料成分影响研究和原料的筛选研究。
3.脂肪酶固定化的研究
对产气肠杆菌和成团肠杆菌发酵液得到的沉淀进行冷冻干燥,采用硅藻土固定化。
产气肠杆菌:硅藻土用量与酶粉用量为10:1,20℃下吸附2 h,得到的固定化酶的活性最大(1111.1 U/g)。该固定化酶在30℃,pH 8.0条件下催化性和稳定性较好。
成团肠杆菌:硅藻土用量与酶粉用量为15.625:1,15℃吸附1.5 h,得到的固定化酶的活性最大(666.7 U/g)。该固定化酶在30℃,pH 8.0条件下催化性和稳定性较好。
4.有机溶剂体下催化生产生物柴油
硅藻土固定化产气肠杆菌脂肪酶用量为1000 U,乙醇为酰基受体(醇:油摩尔比为4:1,2次等量加入),5 mL正己烷,180 r/min,35℃,反应48 h,转化率达92.91%。同时还发现,水对该反应有抑制作用,随着其含量增加,转化率降低;油酸对该反应有促进作用,而芥酸有不利影响。
硅藻土固定化成团肠杆菌脂肪酶用量为1000 U,甲醇为酰基受体,甲醇与油摩尔比为3:1,3次等量加入,5 mL正己烷,180 r/min,35℃反应48 h,转化率到91%。该反应体系可用于催化大豆油和葵花子油生产生物柴油,产率分别为88.57%和83.51%。该体系也可催化含水原料生物柴油,在反应体系中水含量达25%时,仍可获得61.97%的转化率。
该研究取得了如下创新:
(1)筛选得到3株活性较高的脂肪酶产生菌,进行了分类鉴定和培养条件优化:
(2)改进了气相色谱内标法测定生物柴油方法,纠正了前人该方面研究的不足;
(3)在有机溶剂体系下催化生产生物柴油的研究,并得到较高的产率,证实本研究得到脂肪酶的有效性,也为原料筛选提供了参考。
Biodiesel is produced from soybean oil,rapeseed oil and other vegetable oils or animal fats by transesterification with methanol,has attracted considerable attention as a renewable energy.It can not be affected by geographical,environmental and other factors,and can reduce the dependence on the oil market,so it becomes more and more and more attractive.
As the price and adequate supplies of raw materials are the bottleneck of the development of biodiesel,low-cost lipase is also a key factor of enzymatic biodiesel, so,a large number of strains which produce lipase were screened and different rapeseed oils were tested in this study to resolve these problem.
The main points were as follows,
1.25 strains produced lipase were screened and 8 of them have higher activity.Three strains with high lipase productivity were identified;the optimum culture conditions were achieved.
The enzyme activity of Enterobacter aerogenes can reach 34.16 U/mL under the optimum conditions which were:initial pH 9.0,agitating at 30℃for 40 h,with NaHCO_3 and glucose as carbon sources,peptone as nitrogen source,0.1%(w/v) MgSO_4·7H_2O.When adding 1.0%(w/v) olive oil to the culture,the enzyme activity can reach 66.31 U/mL,which has increased by 56.5%compared to that obtained by using 2.0%(w/v) olive oil as the sole carbon source.With a preliminary study,the characteristic of this enzyme were found:the optimum reaction temperature is 30℃, the best reaction pH was 8.0.
Under the optimum conditions determined,the enzyme activity of Enterobacter agglomerans can reached to 39.09 U/mL,and the optimal conditions were found to be: initial pH 7.0,agitating at 30℃for 48 h,with 2.0%(w/v) lactose as carbon sources, 1.5%(w/v) beef extract and 1.0%(w/v) yeast extract as nitrogen source,0.1%(w/v) MgSO_4·7H_2O.Olive oil,sesame oil and tea oil as raw materials can be catalyzed on biodiesel by the lipase of this strain under the conditions of 30℃and 180 rpm.And the yield reached to 54.51%with sesame oil as raw material,even when there contained 92.4%(w/v) water in the starting materials.
The optimum culture conditions of Acinetobacter calcoaceticus were:initial pH 7.0, agitating at 30℃for 24 h,1.5%(w/v) maltose,2.5%(w/v) NH_4Cl,0.1%(w/v) MgSO_4·7H_2O.The optimal medium loading volume was 40 mL/100 mL and the inoculation volume is 1.0%,the enzyme activity reached 9.07 U/mL.
2.A determination method of fatty acid methyl esters in biodiesel by GC was developed and established
A determination method of fatty acid methyl esters in biodiesel by GC was developed and established and the impact of various components of biodiesel was studied.The Linear Equations of the fatty acid methyl esters(methyl palmitate, methyl stearic acid methyl,methyl oleate,methyl linoleate,methyl linolenate,methyl cis-11-eicosenoate) was established based on their different content for the first time, which used methyl heptadecanoate as an internal standard.When the sample of the fatty acid methyl esters was in their own linear range respectively,the linearity relative coefficient was more than 0.999,the average recoveries was from 97.91%to 100.45%,and the relative standard deviations were from 0.28%to 2.22%in the precision experimental,while the present of rapeseed oil has little affection to the determination system.The synthesis reaction degree can be judged accurately by comparing the total content of the fatty acid methyl esters in reaction system,and the determination results are creditable.This method can be used for the determination of biodiesel yield and the exact content of the various components.At the same time it can be used to the screening of raw materials and the effect of different components.
3.The study of immobilized lipases
The lipase of Enterobacter aerogenes and Enterobacter agglomerans were immobilized by diatomite in this study.
The lipase of Enterobacter aerogenes was immobilized by diatomite in this study. When the dosage of enzyme powder compared with diatomite is 10:1,the adsorption time is 2 h,and the adsorption reaction was operated on 20℃,the greatest activity (1111.1 U/g) was obtained.Under the conditions of 30℃,pH 8.0,the immobilized lipase has better catalytic effects and good stability.
The lipase of Enterobacter agglomerans was immobilized by diatomite in this study.When the dosage of enzyme powder compared with diatomite is 15.625:1,the adsorption time is 1.5 h,and the adsorption reaction was operated on 15℃,the greatest activity(666.7 U/g) was obtained.Under the conditions of 30℃,pH 8.0,the immobilized lipase has better catalytic effects and good stability.
4.The transesterification reaction were studied under organic solvents system
The dosage of diatomite immobilized lipase of Enterobacter aerogenes is 1000 U, ethanol as acyl receptors(alcohol:oil molar ratio is 4:1,2 times add),5 mL n-hexane, 180 r/min,35℃,48 h,and the conversion rate of 92.91%.At the same time,we found that the conversion rate decrease with the water content increase,lower conversion rate;it is in favor of high oleic acid content in the production of biodiesel, thus erucic acid have adverse effects.
The dosage of immobilized lipase of Enterobacter agglomerans is 1000 U, methanol as acyl receptors(alcohol:oil molar ratio is 3:1,3 times add),5 mL n-hexane,180 r/min,35℃,48 h,and the conversion rate was 91%.This reaction system can catalyze soybean oil and sunflower oil too,and the conversion rate was 83.51%and 88.57%respectively.It is in favor of high oleic acid content in the production of biodiesel,thus erucic acid have adverse effects.With 25%water content, there also has a conversion rate of 61.97%,so it can be used on waste oils to produce biodiesel.
Achievements of this study:
1.Three strains with high lipase productivity were selected and identified,the optimum culture conditions were achieved;
2.A determination method of fatty acid methyl esters in biodiesel by GC was established and developed;
3.Immobilized lipases catalyzed biodiesel were studied in organic solvents system and obtained higher yield.It tested that the lipases screened in this study can be used on biodiesel production.
原料的价格、供应和廉价的脂肪酶是影响酶法生产生物柴油的关键因素。为解决这些问题,在本研究中,筛选了25株脂肪酶产生菌并对其中效果较好的2个脂肪酶进行催化生产生物柴油的研究,同时研究了原料的影响,为筛选合适的原料提供了参考。
研究内容和结果如下:
1.筛选得到25株脂肪酶菌种,其中8株活性较高,对其中活性较好的3株进行,并种类鉴定、最佳培养条件确定等研究。
产气肠杆菌(Enterobacter aerogenes)以1.0%的葡萄糖和1.5%的NaHCO_3为C源,1.5%的蛋白胨为N源,添加0.1%MgSO_4.7 H_2O,起始pH 9.0,装液量50mL/100 mL,120 rpm,30℃培养48 h,酶活达34.16 U/mL;加入1.0%的橄榄油,酶活可提高到66.31 U/mL。该脂肪酶在30℃,pH 8.0时酶催化活性较高。
成团肠杆菌(Enterobacter agglomerans)以2%的乳糖作为碳源,1.5%的牛肉浸膏和1.0%的酵母膏作为氮源,添加0.1%MgSO_4·7 H_2O,初始pH 7.0,120 r/min,30℃培养48 h时产酶活力达39.09 U/mL。发酵液在30℃,180 r/min时催化性能较强。在反应体系中含水量达91%以上时,仍可催化芝麻油生产生物柴油,产率达54.51%。
乙酸钙不动杆菌(Acinetobacter calcoaceticus),以1.5%的麦芽糖作为碳源,2.5%的NH_4Cl为氮源,初始pH 7.0,接种量1.0%,装液量40 mL/100 mL,120 r/min下30℃培养24 h,酶活达9.07 U/mL。2.确定了气相色谱内标法测定生物柴油方法
以十七酸甲酯为内标,依据菜籽油组分含量不同,改进了气相色谱内标法测定生物柴油方法,建立了棕榈酸甲酯、硬脂酸甲酯、油酸甲酯、亚油酸甲酯、亚麻酸甲酯和花生一烯酸甲酯的线性方程,使各个组分同时处于测量范围内,确保了测定结果的准确性。各脂肪酸甲酯含量在各自线性范围内的相关系数r≥0.999、平均回收率在97.91%-100.45%,精密度高,试验标准偏差在0.28%-2.22%,重复性好。该体系可用于菜籽油合成生物柴油产率测定,也可用于原料成分影响研究和原料的筛选研究。
3.脂肪酶固定化的研究
对产气肠杆菌和成团肠杆菌发酵液得到的沉淀进行冷冻干燥,采用硅藻土固定化。
产气肠杆菌:硅藻土用量与酶粉用量为10:1,20℃下吸附2 h,得到的固定化酶的活性最大(1111.1 U/g)。该固定化酶在30℃,pH 8.0条件下催化性和稳定性较好。
成团肠杆菌:硅藻土用量与酶粉用量为15.625:1,15℃吸附1.5 h,得到的固定化酶的活性最大(666.7 U/g)。该固定化酶在30℃,pH 8.0条件下催化性和稳定性较好。
4.有机溶剂体下催化生产生物柴油
硅藻土固定化产气肠杆菌脂肪酶用量为1000 U,乙醇为酰基受体(醇:油摩尔比为4:1,2次等量加入),5 mL正己烷,180 r/min,35℃,反应48 h,转化率达92.91%。同时还发现,水对该反应有抑制作用,随着其含量增加,转化率降低;油酸对该反应有促进作用,而芥酸有不利影响。
硅藻土固定化成团肠杆菌脂肪酶用量为1000 U,甲醇为酰基受体,甲醇与油摩尔比为3:1,3次等量加入,5 mL正己烷,180 r/min,35℃反应48 h,转化率到91%。该反应体系可用于催化大豆油和葵花子油生产生物柴油,产率分别为88.57%和83.51%。该体系也可催化含水原料生物柴油,在反应体系中水含量达25%时,仍可获得61.97%的转化率。
该研究取得了如下创新:
(1)筛选得到3株活性较高的脂肪酶产生菌,进行了分类鉴定和培养条件优化:
(2)改进了气相色谱内标法测定生物柴油方法,纠正了前人该方面研究的不足;
(3)在有机溶剂体系下催化生产生物柴油的研究,并得到较高的产率,证实本研究得到脂肪酶的有效性,也为原料筛选提供了参考。
Biodiesel is produced from soybean oil,rapeseed oil and other vegetable oils or animal fats by transesterification with methanol,has attracted considerable attention as a renewable energy.It can not be affected by geographical,environmental and other factors,and can reduce the dependence on the oil market,so it becomes more and more and more attractive.
As the price and adequate supplies of raw materials are the bottleneck of the development of biodiesel,low-cost lipase is also a key factor of enzymatic biodiesel, so,a large number of strains which produce lipase were screened and different rapeseed oils were tested in this study to resolve these problem.
The main points were as follows,
1.25 strains produced lipase were screened and 8 of them have higher activity.Three strains with high lipase productivity were identified;the optimum culture conditions were achieved.
The enzyme activity of Enterobacter aerogenes can reach 34.16 U/mL under the optimum conditions which were:initial pH 9.0,agitating at 30℃for 40 h,with NaHCO_3 and glucose as carbon sources,peptone as nitrogen source,0.1%(w/v) MgSO_4·7H_2O.When adding 1.0%(w/v) olive oil to the culture,the enzyme activity can reach 66.31 U/mL,which has increased by 56.5%compared to that obtained by using 2.0%(w/v) olive oil as the sole carbon source.With a preliminary study,the characteristic of this enzyme were found:the optimum reaction temperature is 30℃, the best reaction pH was 8.0.
Under the optimum conditions determined,the enzyme activity of Enterobacter agglomerans can reached to 39.09 U/mL,and the optimal conditions were found to be: initial pH 7.0,agitating at 30℃for 48 h,with 2.0%(w/v) lactose as carbon sources, 1.5%(w/v) beef extract and 1.0%(w/v) yeast extract as nitrogen source,0.1%(w/v) MgSO_4·7H_2O.Olive oil,sesame oil and tea oil as raw materials can be catalyzed on biodiesel by the lipase of this strain under the conditions of 30℃and 180 rpm.And the yield reached to 54.51%with sesame oil as raw material,even when there contained 92.4%(w/v) water in the starting materials.
The optimum culture conditions of Acinetobacter calcoaceticus were:initial pH 7.0, agitating at 30℃for 24 h,1.5%(w/v) maltose,2.5%(w/v) NH_4Cl,0.1%(w/v) MgSO_4·7H_2O.The optimal medium loading volume was 40 mL/100 mL and the inoculation volume is 1.0%,the enzyme activity reached 9.07 U/mL.
2.A determination method of fatty acid methyl esters in biodiesel by GC was developed and established
A determination method of fatty acid methyl esters in biodiesel by GC was developed and established and the impact of various components of biodiesel was studied.The Linear Equations of the fatty acid methyl esters(methyl palmitate, methyl stearic acid methyl,methyl oleate,methyl linoleate,methyl linolenate,methyl cis-11-eicosenoate) was established based on their different content for the first time, which used methyl heptadecanoate as an internal standard.When the sample of the fatty acid methyl esters was in their own linear range respectively,the linearity relative coefficient was more than 0.999,the average recoveries was from 97.91%to 100.45%,and the relative standard deviations were from 0.28%to 2.22%in the precision experimental,while the present of rapeseed oil has little affection to the determination system.The synthesis reaction degree can be judged accurately by comparing the total content of the fatty acid methyl esters in reaction system,and the determination results are creditable.This method can be used for the determination of biodiesel yield and the exact content of the various components.At the same time it can be used to the screening of raw materials and the effect of different components.
3.The study of immobilized lipases
The lipase of Enterobacter aerogenes and Enterobacter agglomerans were immobilized by diatomite in this study.
The lipase of Enterobacter aerogenes was immobilized by diatomite in this study. When the dosage of enzyme powder compared with diatomite is 10:1,the adsorption time is 2 h,and the adsorption reaction was operated on 20℃,the greatest activity (1111.1 U/g) was obtained.Under the conditions of 30℃,pH 8.0,the immobilized lipase has better catalytic effects and good stability.
The lipase of Enterobacter agglomerans was immobilized by diatomite in this study.When the dosage of enzyme powder compared with diatomite is 15.625:1,the adsorption time is 1.5 h,and the adsorption reaction was operated on 15℃,the greatest activity(666.7 U/g) was obtained.Under the conditions of 30℃,pH 8.0,the immobilized lipase has better catalytic effects and good stability.
4.The transesterification reaction were studied under organic solvents system
The dosage of diatomite immobilized lipase of Enterobacter aerogenes is 1000 U, ethanol as acyl receptors(alcohol:oil molar ratio is 4:1,2 times add),5 mL n-hexane, 180 r/min,35℃,48 h,and the conversion rate of 92.91%.At the same time,we found that the conversion rate decrease with the water content increase,lower conversion rate;it is in favor of high oleic acid content in the production of biodiesel, thus erucic acid have adverse effects.
The dosage of immobilized lipase of Enterobacter agglomerans is 1000 U, methanol as acyl receptors(alcohol:oil molar ratio is 3:1,3 times add),5 mL n-hexane,180 r/min,35℃,48 h,and the conversion rate was 91%.This reaction system can catalyze soybean oil and sunflower oil too,and the conversion rate was 83.51%and 88.57%respectively.It is in favor of high oleic acid content in the production of biodiesel,thus erucic acid have adverse effects.With 25%water content, there also has a conversion rate of 61.97%,so it can be used on waste oils to produce biodiesel.
Achievements of this study:
1.Three strains with high lipase productivity were selected and identified,the optimum culture conditions were achieved;
2.A determination method of fatty acid methyl esters in biodiesel by GC was established and developed;
3.Immobilized lipases catalyzed biodiesel were studied in organic solvents system and obtained higher yield.It tested that the lipases screened in this study can be used on biodiesel production.
引文
[1]翟秀静,刘奎仁,韩庆.新能源技术[M].北京,化学工业出版社,2005年9月第一版
[2]Yasuhisa A,Hideki F,Yuji S,et al.Biofuel Production Process by Novel Biocatalysts[J].Journal of Molecular Catalysis B:Enzymatic,2002,17(3-5):111
[3]邓利,谭天伟,王芳.脂肪酶催化合成生物柴油的研究[J].生物工程学报,2003,19(1):97-101
[4]巫淼鑫,邬国英,韩瑛等.6种食用植物油及其生物柴油中脂肪酸成分的比较研究[J].中国油脂,2003,28(12):65-67
[5]杨伟华,许红霞,王延琴等.应用棉子油生产生物柴油的可行性分析[J].中国棉花·棉区了望,2007,34(1):41-42
[6]张欢,孟永彪.用棉籽油制备生物柴油[J].化工进展,2007,26(1):86-89
[7]邬国英,林西平,巫淼鑫等.棉籽油甲酯化联产生物柴油和甘油[J].中国油脂,2003,28(4):70-73
[8]马俊林,郭俊宝,徐一辉等.大豆油制备生物柴油的工艺探索[J].可再生能源,2006,2:34-36
[9]盛梅,郭登峰,张大华等.大豆油制备生物柴油的研究[J].中国油脂,2002,27(1):70-72
[10]何东平,张世宏,齐玉堂等.生物柴油生产技术研究[J].武汉工业大学学报,2003,23(4):52-54
[11]周慧,鲁厚芳,唐盛伟等.麻疯树油制备生物柴油的酯交换工艺研究[J].应用化工,2006,35(4):284-287
[12]韦公远.用米糠油制取生物柴油[J].西部粮油科技,2006,26(1):51-52
[13]王蓉辉,曹祖宾,王亮等.葵花籽油制备生物柴油的研究[J].广州化工,2006,36(1):35-37
[14]G.Antolin,F.V.Tinaut,Y.Briceno,et al.Optimisation of biodiesel production by sunflower oil transesterification[J].Bioresource Technology.2002,83:111-114
[15]刘伟伟,苏有勇,张无敌等.橡胶籽油制备生物柴油的研究[J].中国油脂,2005,30(10):63-66
[16]苏有勇,刘士清,张无敌等.小桐油制备生物柴油的研究[J].新能源及工艺,2006,1:22-26
[17]马传国,司耀彬,侯华锋.皂脚制备生物柴油的研究[J].中国油脂,2006,31(4):59-61
[18]金付强,张建春,杨儒等.大麻籽油合成生物柴油[J].应用化学,2007,24 (1):100-104
[19]Yuji S,Yomi W,Akio S,et al.Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing[J].Journal of Molecular Catatlysis B:Enzymatic,2002,17:133-142
[20]庾乐.非水相体系中酶催化制备生物柴油[D].南宁:广西大学,2006
[21]高静,王芳,谭天伟等.固定化脂肪酶催化废油合成生物柴油[J].化工学报,2005,56(9):1727-1730
[22]陈志锋,吴虹,宗敏华.固定化脂肪酶催化高酸废油脂酯交换生产生物柴油[J].催化学报,2006,27(2):146-150
[23]聂开立,王芳,邓利等.间歇及连续式固定化酶反应生产生物柴油[J].生物加工过程,2005,3(1):58-62
[24]Ana VLP,Enoch YP.Lipase-catalyzed production of Biodiesel fuel from vegetable oils contained in waste activated bleaching earth[J].Process Biochemistry,2003,38:1077-1082
[25]Enoch YP,Masahiro M.Kinetic study of esterification of rapeseed oil contained in waste activated bleaching earth using Candida rugosa lipase in organic solvent system[J].Journal of Molecular Catatlysis B:Enzymatic,2005,37:95-100
[26]Vanessa PL,Enoch YP.Potential application of waste activated bleaching earth on the production of fatty acid alkyl esters using Candida cylindracea lipase in organic solvent system[J].Enzyme and Microbial Technology,2004,34:270-277
[27]Mohamed M S,Uwe T B.Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil[J].Enzyme and Microbial Technology,2003,33:97-103
[28]Wu H,Zong MH,Lou WY.Transesterification of waste oil to biodiesel in solvent free system catalyzed by immobilized lipase[J].Chinese journal of Catalysis,2004,25(11):903-909
[29]John S,Terri D,John B,et al.A Look Back at the U.S.Department of Energy's Aquatic Species Program-Biodiesel from Algae[R].Prepared for:U.S.Department of Energy's Office of Fuels Development,1998
[30]里伟,杜伟,李永红等.生物酶法转化酵母油脂合成生物柴油[J].过程工程学报,2007,7(1):137-140
[31]刘会丽.Novo435酶催化植物油制取生物柴油酯交换反应条件的试验研究[D].郑州:河南农业大学,2006
[32]Veena K,Shweta S,Munishwar N G.Preparation of Biodiesel by Lipase-Catalyzed Transesterification of High Free Fatty Acid Containing Oil from Madhuca indica[J].Energy & Fuels,2007,21:368-372
[33]Kamini NR,Iefuji H.Lipase catalyzed methanolysis of vegetable oils in aqueous medium by Cryptococcus spp.S-2[J].Process Biochemistry,2001,37:405-410
[34]Shweta S,Munishwar N G.Lipase catalyzed preparation of biodiesel from Jatropha oil in a solvent free system[J].Process Biochemistry,2007,42:409-414
[35]Shah S,Sharma S,Gupta M N.Biodiesel Preparation by Lipase-Catalyzed Transesterification of Jatropha Oil[J].Energy & Fuels,2004,18:154-159
[36]倪婉星,张晓鸣.非水相脂肪酶催化合成生物柴油[J].食品与机械,2007,23(1):25-28
[37]陈文伟.乌柏梓油制备生物柴油的研究[D].南昌:南昌大学,2006
[38]田春龙,郭斌,刘春朝.能源植物研究现状和展望[J].生物加工过程,2005,3(1):14-18.
[39]巫淼鑫,邬国英,韩立峰等.食用植物油所制备生物柴油的低温流动性能[J].石油炼制与化工,2005,35(4):57-60.
[40]谢立华,李培武,张文等.油菜作为优势能源作物的发展潜力与展望[J].生物加工过程,2005,3(1):28-31.
[41]王汉中.发展油菜生物柴油的潜力、问题和对策[J].中国油料作物学报,2005,27(2):74-76.
[42]王汉中.实施油菜生物柴油计划建造永不枯竭绿色油田[J].中国农村科技,2005.10:40-41.
[43]张书芬,朱家成,王建平等.选育高油高产油菜品种,大力发展油菜生物柴油[J].河南农业科学,2006,9:52-55.
[44]Breda K.Experimental Investigation of Optimal Timing of the Diesel Engine Injection Pump Using Biodiesel Fuel[J].Energy & Fuels,2006,20:1460-1470.
[45]Leung DYC,Luo Y,Chan T L.Optimization of Exhaust Emissions of a Diesel Engine Fuelled with Biodiesel[J].Energy & Fuels,2006,20:1015-1023.
[46]Tsolakis A.Effects on Particle Size Distribution from the Diesel Engine Operating on RME-Biodiesel with EGR[J].Energy & Fuels,2006,20:1418-1424.
[47]盛梅,李为民,邬国英.生物柴油研究进展[J].中国油脂,2003,28(4):66-70.
[48]李为民,章文峰,邬国英.菜籽油油脚制备生物柴油[J].江苏工业学院学报,2003,15(1):7-10.
[49]Peterson CL,Feldman M,Korus R,et al.Batch type tranesterification process for winter rape oil[J].Applied Engineenng In Agriculture.1991,7(6):711-716.
[50]Barn,Narendra.Process development of rapeseed oil ethyl ester as a dieselfirel substitute[R].Department of Chemical Engineering,University of Idaho,U.S.A.,1991.
[51]Komers K,Machek J,Stloukal R.Biodiesel from rapeseed oil,methanol and KOH.2.Composition of solution of KOH in methanol as reaction partner of oil[J].Eur.J.Lipid Sci.Technol.,2001,103:359-362.
[52]Komers K,Stloukal R,Machek J.Biodiesel from rapeseed oil,methanol and KOH.3.Analysis of composition of actual reaction mixture[J].Eur.J.Lipid Sci.Technol.,2001,103:363-371.
[53]Komers K,Stloukal R,Machek J.Kinetics and mechanism of the KOH-catalyzed methanolysis of rapeseed oil for biodiesel production[J].Eur.J.Lipid Sci.Technol.,2002,104:728-737.
[54]Hemmerlein M,Korte V,Richter H S.Performance,exhaust emission and durability of modem diesel engines running on rapeseed oil[J].SAE Paper,1991,910848.
[55]Recep A,Selim C,H(u|¨)seyin S,et al.The potential of using vegetable oil fuels as fuel for diesel engines[J].Energy Conversion and Management,2001,42:529-538.
[56]Charles L,Peterson,Daryl L,et al.Ethyl ester of rapeseed used as a biodiesel fuel-a case study[J].Biomass and Bioenergy,1996,10:331-336.
[57]邬国英,巫森鑫,林西平等.植物油制备生物柴油[J].江苏石油化工学院学报,2002,12(3):8-11.
[58]盛梅,邬国英,徐鸽等.生物柴油的制备[J].高校化学工程学报,2004,18(2):231-236.
[59]雷猛,许世海,魏小平.菜籽油制备生物柴油及其理化性能的研究[J].后勤工程学院学报,2004,4:59-63.
[60]黄庆德,黄凤洪,郭萍梅.生物柴油生产技术及其开发意义[J].粮食与油脂,2002,9:8-10
[61]Roger A,Korus,Dwight S.Transesterification Process to Manufacture Ethyl Ester of Rape Oil[R].Proceedings of 1st Biomass Conference of the Americas,biodieselgear.com,1992.
[62]张冠杰.利用滴流床反应器制备生物柴油的研究[D].南京:南京工业大学,2004.
[63]徐元浩.生物柴油的实用性研究[D].武汉:武汉理工大学,2005.
[64]张无敌,刘士清,尹芳等.菜籽油酯交换制备生物柴油的工艺研究[J].农业工程学报,2006,22(11):131-134.
[65]李为民,邬国英,徐鸽等.菜籽油制备生物柴油放大实验研究[J].江苏工业学院学报,2005,17(2):9-12.
[66]陈文,王存文,张圣利.碱催化酯交换法制备生物柴油的研究[J].化学与生物工程,2007,24(1):38-40.
[67]蒋大勇,郭和军,张红云等.一种新型菜籽油酯生物柴油的制备[J].可再生 能源,2007,25(1):32-35.
[68]杨凯华.天然产物油脂制备生物柴油新技术的研究[D].北京:中国林业科学研究院,2005.
[69]杨军峰,王忠,孙平等.双低菜籽油制备生物柴油的工艺探索[J].柴油机Diesel Engine,2003,3:43-45.
[70]王鹏.生物柴油的制备及相关研究[D].北京:中国农业大学,2006.
[71]Gryglewicz S.Rapeseed Oil Methyl Esters Preparation Using Heterogeneous Catalysts[J].Bioresour Technol,1999,70(3):249-253.
[72]王广欣,颜姝丽,周重文等.用于生物柴油的钙镁催化剂的制备及其活性评价[J].中国油脂,2005,30(10):66-69.
[73]李为民,郑晓林,徐春明等.固体碱法制备生物柴油及其性能[J].化工学报,2005,56(4):711-716.
[74]Tanja C,Siegfried P,Eckhard W.Biodiesel-Transesterification of Biological Oils with Liquid Catalysts:Thermodynamic Properties of Oil-Methanol-Amine Mixtures[J].Ind.Eng.Chem.Res.2005,44(25):9535-9541.
[75]Kusdiana D,Saka S.Effects of water on biodiesel fuel production by supercritical methanol treatment[J].Bioresour.Technol,2004,91:289-295.
[76]Mittelbach M,Silberholz A,Koncar M.In Novel aspects concerning acid-catalyzed alcoholysis of triglycerides[J].Oils-Fats-Lipids 1995,Proceedings of the 21st World Congress of the International Society for Fats Research,The Hague,1995,1996:497-499.
[77]Samukawa T,Kaieda M,Matsumoto T,et al.Retreament of immobilized Candida antarctica lipase for biodiesel fuel production fiom plant oil[J].J.Biosci.Bioeng,2000,90:180-183.
[78]Watanabe Y,Shimada Y,Sugihara A,et al.Conversion of degummed soybean oil to biodiesel fuel with immobilized Candidn arttarctical lipase[J].J.Molecular Catalysis B:Enzymatic,2002,17:151-155.
[79]Mohamed M,Soumanou,Uwe T.Ornschacer.Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil[J].Enzyme and Microbial Technology,2003,33:97-103.
[80]Watanabe Y,Shimada Y,Sugihara A,et al.Continuous production of biodiesel fuel from vegetable oil using immobilized Candida antarctica lipase[J].J.Am.Oil Chem.Soc.,2000,77:355-360.
[81]徐桂转,刘会丽,张百良等.利用菜籽油酶法生产生物柴油的初步研究[J].农业工程学报,2006,22(8):162-165.
[82]盛梅,郭登峰.固定化酶催化菜籽油合成生物柴油稳定性研究[J].中国油 脂,2005,30(5):68-70.
[83]盛梅,杨文伟,郭登峰.固定化脂肪酶催化合成生物柴油[J].应用化学,2005,22(7):788-791.
[84]蔡志强,邬国英,林西平等.固定化脂酶催化合成生物柴油的研究[J].中国油脂,2004,29(8):29-32.
[85]李俐林,杜伟,刘德华等.乙酸甲酯萃取菜籽油制备生物柴油的研究[J].食品与发酵工业,2006,32(5):5-8.
[86]Uosukainen E,L(a|¨)ms(a|¨) M,Linko Y-Y,et al.Optimization of enzymatic transesterification of rapeseed oil ester using response surface and principal component methodology[J].Enzyme and Microbial Technology,1999,25:236-243.
[87]Linko Y-Y,Tervakangas T,L(a|¨)ms(a|¨) M,et al.Production of trimethylolpropane esters of rapeseed oil fatty acids by immobilized lipase[J].Biotechnology Techniques,1997,11(12):889-892.
[88]Linko Y-Y,Tervakangas T,Huhtala A,et al.Lipase biocatalysis in the production of esters[J].Journal of the American Oil Chemists' Society,1995,72(11):1293-1299.
[89]Lamsa,Merja,Linko,et al.Enzymatic process for preparing a synthetic ester from a vegetable oil[P].US:5 747 434,1998-05-05.
[90]Ayhan D.Biodiesel from vegetable oils via transesterification in supercritical methanol[J].Energy Conversion and Management,2002,43:2349-2356.
[91]Kusdiana D,Saka S.Biodiesel fuel from rapeseed oil as prepared in supercritical methanol[J].Fuel,2001,80(20):225-231.
[92]Hiroaki I,Eiji M,Shusaku H,et al.Thermal Stability of Biodiesel Fuel as Prepared by Supercritical Methanol Process[R].The 2nd Joint International Conference on "Sustainable Energy and Environment(SEE 2006)",2006,11:21-23.
[93]Yuichiro W,Dadan K,Shiro S.Reactivity of triglycerides and fatty acids of rapeseed oil in supercritical alcohols[J].Bioresource Technology,2001,91(3):283-287.
[94]Kusdiana D,Saka S.Kinetics of transesterification in rapeseed oil to biodiesel fuel as treated in supercritical methanol[J].Fuel,2001,80(20):693-698.
[95]赵宗保,华艳艳,刘波.中国如何突破生物柴油产业的原料瓶颈[J].中国生物工程杂志,2005,25(11):1-6.
[96]王涛.中国主要生物质燃料油木本能源植物资源概况与展望[J].科技导报,2005,23(5):12-14.(119)
[97]Gerhard K.Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters[J].Fuel Processing Technology,2005,86:1059-1070.
[98]王小行.新型全细胞催化剂在麻风树生物柴油中的应用[D].成都:四川大学博士研究生毕业论文,2006.
[99]Mamoru I,Chen BX,Eguchi M,et al.Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase[J].Journal of Molecular Catalysis B Enzymatic,2001,16(1):53-58.
[100]Kaieda M,Samukawa T,Kondo A,et al.Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system[J].J Biosci Bioeng,2001,91(1):12-15.
[101](O|¨)znur K,Melek T,Ayse AH.Immobilized Candida Antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium[J].Bioresour Technol.,2002,83:125-129.
[102]余琼,梁运祥.产低温脂肪酶假单胞菌的选育及产酶条件的优化[J].湖北农业科学,2006,45(5):662-665
[103]张金伟,曾润颖.南极产低温脂肪酶菌株Psychrobacter sp.7195的选育、发酵条件及酶学性质研究[J].生物磁学,2006,6(1):6-10
[104]Frederic M.T.,章克昌.从非洲土壤中分离新的产生脂肪酶的细菌[J].无锡轻工大学学报,2000,19(1):18-22
[105]雷晓燕.土壤中产碱性脂肪酶霉菌的筛选[J].沈阳化工学院学报,2006,20(1):9-12
[106]宋欣,魏留梅,刘瑞田等.脂肪酶高产菌株选育和菌种库的建立[J].微生物学通报,2002,29(I):6-10
[107]卢世琦,刘光烨,江跃林等.合成己酸乙酯脂肪酶产生菌的筛选及产酶条件[J].微生物学通报,1994,21(1):23-26
[108]宋欣,曲音波,叶寒青等.具脂肪酶和酯酶活性酵母菌的选育和酶学性质初步研究[J].应用与环境生物学报,1999,5(6):628-631
[109]王乐恒.具脂肪酶和酯酶活性酵母菌的选育及酶学研究[J].青岛建筑工程学院学报,2004,25(2):75-77
[110]Gorokhova IV,Ivanov AE,Zaitsev S.Yu,et al.Study of the interaction and activation of lipase from Pseudomonas fluorescens in surfactant monolayers and precipitates.Russ Chem.Bull,2003,52(4):961-969
[111]吕飒音,胡磊.凝结芽胞杆菌产碱性脂肪酶的条件研究[J].安徽大学学报:自然科学版,2004,28(1):69-72
[112]逢淑召,王群,王智等.荧光假单孢菌Pseudomonas fluorescence 5963产脂肪酶条件的优化[J],生物加工过程,2004,2(1):62-65
[113]Kohno M,Funatsu J,Mikami B,et al.The crystal structure of lipase Ⅱ from Rhizopus niveus at 2.2 A resolution[J].J Biotechem,1996,120:505-510.
[114]Washida M,Takahashi S,Ueda M,et al.Spacer-mediated display of active lipase on the yeast cell surface[J].Appl Microbiol Biotechnol,2001,56:681-686.
[115]Takahashi S,Ueda M,Tanaka A.Function of the prosequence for in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae[J].Appl Microbiol Biotechnol,2001,55:454-462.
[116]徐岩,章克昌,王亚非.微生物脂肪酶在正庚烷中合成短链芳香酯的研究[J].生物工程学报,1998,14(2):214-219
[117]Joerger RD,Haas MJ.Alteration of chain length selectivity of a Rhizopus delemar lipase through site-directed mutagenesis[J].Lipids,1994,29:377-384
[118]Klein RR,King G,Moreau RA,et al.Additive effects of acyl-binding site mutations on the fatty acid selectivity of Rhizopus delemar lipase[J].JAOCS,1997,74(11):1401-1407
[119]侯红漫,吴韬,王运吉.产脂肪酶根霉的诱变及其酶促酯化反应的研究[J].大连轻工业学院学报,1996,15(1):138-141
[120]张冬花,王运吉.高产脂肪酶菌株的诱变筛选[J].生物技术,1996,6(2):20-22
[121]周延,张鹏,孟庆云.用快中子法选育细菌脂肪酶高产菌株[J].微生物学通报,2001,28(2):81-85
[122]黄娟,陈正杰,冯军等.紫外和微波结合诱变选育lipstatin产生菌[J].中国医药工业杂志,2006,37(1):12-14
[123]Jinhong Zhang,Zhibo Hou,Chuanyi Yao,et al.Purilicalion and properlies of lipase from a Bacillus drain for calalylic resolution of(R)-Naproxen.Journal of Molecular Catalvis B:Enzymatic.2002,18:205-210
[124]NR Kamini,T Fujii,T Kurosu,et al.Production,purification and characterization of an extracellular lipase from the yeast,Cryptococcus sp.S-2.Process Biochemistry.2000,36:317-324
[125]N Krieger,MA Taipa,EHM Melo,et al.Purification of a Penicillium citrinum lipase by chromatographic processes.Bioprosses Engineering.1999,20:59-65
[126]闫云君,陈晖,杨江科等.ATPS法分离纯化Aspergillus sp.F044脂肪酶[J].华中科技大学学报(自然科学版),2005,33(10):92-95
[127]辛嘉英,徐毅,李树本等.Candida rugosa脂肪酶同工酶的分离及选择固定化[J].生物化学与生物物理进展,2000,27(5):513-516
[128]邬敏辰,刘为平,许云敏等.碱性脂肪酶的高效液相色谱和质谱分析[J].无锡轻工大学学报,2000,19(5):158-161
[129]吴吟妮,潘剑茹,江贤章等.嗜硫色谱分离纯化碱性脂肪酶及其氨基酸序列测定[J].工业微生物,2004,34(4):158-161
[130]Marttin N,Joha B,Erland H,el al.Lipase immobilized by adsorption[J].Appl Microbiol Biotechnol,1988,28:527-530
[131]Tsuneo Y.Repeated use of lipase immobilized on amphiphilic gel for hydrolysis of a small amount of glycerides in eluded in liquid crude fatty acid[J].Ferment Technol,1982,60(6):517-523
[132]张军,徐家立.固定化假丝酵母1619脂肪酶催化油酸油醇酯的合成[J].生物工程学报,1995,11(4):325-331
[133]刘新喜,彭立凤.蛋壳作载体固定化脂肪酶[J].固原师专学报(自然科学版),2000,1(6):21-24
[134]高阳,谭天伟,聂开立等.大孔树脂固定化脂肪酶及在微水相中催化合成生物柴油的研究[J].生物工程学报,2006,22(1):114-118
[135]陈秀琳,郑毅,王绍钊等.CM-葡聚糖凝胶固定化脂肪酶的研究[J].药物生物技术,1996,6(1):20-23
[136]陈秀琳.CM-纤维素固定化脂肪酶的研究[J].Strait Pharmaceutical Journa,2005,17(1):35-37
[137]徐慧显,李民勤,王道宾等.聚丙烯酸甲酯载体对柱状假丝酵母脂肪酶固定化的研究(Ⅰ)[J].离子交换与吸附,1994,10(4):316-321
[138]谭天伟,王芳,刘天全.用硅藻土固定化脂肪酶及酶法合成单甘油酯[J].中国粮油学报,2000,15(2):29-31
[139]Sailas Benjamin,Ashok Pandey.Enhancement of lipase production during repeated batch culture using immobilized Candida rugosa[J].Process Biochemistry,1997,32(5):437-440
[140]赵庆节,余莹.有机相中大孔吸附树脂固定化酶催化拆分消旋萘普生[J].华东理工大学学报:自然科学版,2001,27(40):423-426.
[141]Wu XY,Jaaskelainen S,Linko YY.An investigation of crude lipases for hydrolysis,esterification and transesterification[J].Enzyme Microb.Technol.1996,19:226-231
[142]Shaw JF,Chang R.C.Lipolytic Activities of a Lipase Immobilization on Six Selected Supporting Materials[J].Biotechnology and Bioengineering,1990,35:132-137
[143]安永磊,唐唯森,高松.酶法催化餐饮废油制备生物柴油的研究[J].吉林大学学报(地球科学版),2006,36增刊:147-150.
[144]徐圆圆,杜伟,刘德华.非水相脂肪酶催化大豆油脂合成生物柴油的研究[J].现代化工,2003,23卷增刊:167-169.
[145]曾淑华,周位,杨江科等.固定化脂肪酶催化大豆油制备生物柴油[J].食品与生物技术学报,2007,26(3):75-79.
[146]王学伟,常杰,吕鹏梅等.固定化脂肪酶催化制备生物柴油[J].石油化工,2005,34(9):855-858.
[147]聂开立,王芳,谭天伟.固定酶法生产生物柴油[J].现代化工,2003,23(9):35-38.(104)
[148]鲁明波,余龙江,薛勇等.生物柴油的酶促合成研究[J].中国油脂,2005,30(11):62-64.
[149]薛勇.微生物脂肪酶及其催化合成生物柴油的研究[D].武汉:华中科技大学硕士研究生毕业论文,2004.
[150]李堂,黄朋,周建红等.正交优化脂肪酶催化菜籽油制备生物柴油[J].湘潭师范学院学报(自然科学版),2007,29(2):22-25.
[151]Noureddini H,Gao X,Philkana RS.Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil[J].Bioresource Technology,2005,96:769-777.
[152]Liu Y,Kondo A,Ohkawa H,et al.Bioconversion Using Immobilized Recombinant Flocculent Yeast Cells Carryinga Fused Enzyme Gene in an "Intelligent" Bioreactor[J].Biochem Eng J,1998,2(4):229-235
[153]Fukuda H.,Kondo A.,Noda H.Biodiesel Fuel Production by Transesterification of Oils[J].J Biosci Bioeng,2001,92(5):405-416
[154]Matsumoto T,Takahashi S,Kaieda M,et al.Yeast Whole-cell Biocatalyst Constructed by Intracellular Overproduction of Rhizopus Oryzaelipase is Applicable to Biodiesel Fuel Production[J].Appl Microbiol Biotechnol,2001,57(4):515-520
[155]Matsumoto T.,Takahashi S.,Ueda M.,et al.Preparation of High Activity Yeast Whole Cell Bioctalysts by Optimization of Intracellular Production of Recombinant Rhizopus Oryzae Lipase[J].Journal of Molecular Catalysis B:Enzymatic,2002,17(3-5):143-149
[156]Kazuhiro B,Shinji H,Keiko N,et al.Repeated use of whole-cell biocatalysts immobilized with biomass support particles for Biodiesel fuel production[J].Journal of Molecular Catatlysis B Enzymatic,2002(17):157-165.
[157]Kazuhiro Ban,Shinji Hama,Keiko Nishizuka,et al.Repeated Use of Whole-cell Biocatalysts Immobilized within Biomass Support Particles for Biodiesel Fuel Production[J].Journal of Molecular Catalysis B:Enzymatic,2002,17(3-5):157-165
[158]Matsumoto T,Fukuda H,Ueda M,et al.Construction of Yeast Strains with High Cell Surface Lipase Activity by Using Novel Display Systems Based on the Flolp Flocculation Functional Domain[J].Applied and Enviromental Microbiology,2002,68(9):4517-4522
[159]Shinji H,Sriappareddy T,Takahiro F,et al.Lipase Localization in Rhizopus oryzae Cells Immobilized within Biomass support Particles for Use as Whole-Cell Biocatalysts in Biodisel-Fuelc Production[J].J.bioscience and bioengineering,2006,101(4):328-333
[160]Ban K,Kaieda M,Matsumoto T,et al.Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles[J].Biochem Eng J,2001,8(1):39-43.
[161]Dossat V,Combes D,Marty A.Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor:influence of the glycerol production[J].Enzyme Microbial Technol.,1999,25:194-200.
[162]Kaili N,Feng X,Fang W,et al.Lipase catalyzed methanolysis to produce biodiesel[J].Journal of Molecular Catalysis B:Enzymatic,2006,43:142-147.
[163]张振乾,官春云.酶催化法合成生物柴油发展现状及对策[J].中国生物柴油,2008,2:16-20
[164]胡爱军,邓捷.超声波辐射对酶法制备生物柴油的影响[J].天津科技大学学报,2007,22(1):29-32.
[165]王学伟.假丝酵母脂肪酶醇解制备生物柴油的研究[D].北京:北京化工大学硕士研究生毕业论文,2006.
[166]陈天祥,吴邦信.菜籽油芥酸提取甲酯化工艺研究[J].贵州化工,1996,(4):12-15
[167]章亭洲,杨立荣,朱自强.脂肪酶改性对其在有机相中催化特性的影响.浙江大学学报(农业与生命科学版).2001,27(3):237-243
[168]En-Shyh Lin,Chee-Chan Wang,Shu-Chiao Sung.Cultivating conditions influence lipase production by the edible Basidiomycete Antrodia cinnamomea in submerged culture[J].Enzyme and Microbial Technology,2006,39:98-102
[169]Shinji H,Hideki Y,Masaru K,et al.Effect of fatty acid membrane composition on whole-cell biocatalysts for biodiesel-fuel production[J].Biochemical Engineering Journal,2004,21:155-160
[170]MMD Maia,A Heasley,MM Camargo de Morais,et al.Effect of culture conditions on lipase production by Fusarium solani in batch fermentation[J].Bioresource Technology,2001,76:23-27
[171]JA Rodriguez,JC Mateos,J Nungaray,et al.Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation[J].Process Biochemistry,2006,41:2264-2269
[172]Yao-Qiang He,Tian-Wei Tan.Use of response surface methodology to optimize culture medium for production of lipase with Candida sp.99-125[J].Journal of Molecular Catalysis B:Enzymatic,2006,43:9-14
[173]Tsuchiya K,Tada S,Gomi K,et al.High level expression of the synthetic human lysozyme gene in Aspergillus oryzae[J].Appl Micrdbiol Biotechnol,1992,38:109-114.
[174]Zheng XF,Kobayashi Y,Takeuchi M.Construction of a low-serinetype -carboxypeptidase-producing mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion[J].Appl Microbiol Biotechnol,1998,49:39-44.
[175]JL Mau,JY Li.Effect of 10-oxo-trans-8-decenoic acid on the growth and enzyme activities of Aspergillus niger and A.oryzae[J].Fungal Science.2002,17(4):65-76.
[176]HP Chen,KF Hsiao,SH Wu.Regioselectivity Enhancement by Partially Purified Lipase from Aspergillus Niger[J].Biotechnol.Lett.1995,17:305-308.
[177]Chen Jyh-Ping,Mcgill SD.Enzymatic hydrolysis of triglycerides by Rhizopus delemar immobilized on biomass support particles[J].Food Biotechnol,1992,6(1):1-18
[178]Elibol M,Ozer D.Lipase production by immobilized Rhizopus arrhizus[J].Process Biochem,2000,36:219-223
[179]Salleh AB,Musani R,Basri M,et al.Extra-and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production[J].Can J Microbiol.1993.39:978-981
[180]邬敏辰,孙崇荣,乌显章等.平板扩散法粗略确定碱性脂肪酶的活性[J].无锡轻工大学学报,2000,19(2):168-172
[181]Abramic M,Lescic I,Korica T,et al.Purification and properties of extracellular lipase from Streptomyces rimosus[J].Enzyme and Microb Technol,1999,25:522-529
[182]王树宁,高晗,孙俊良等.不同诱变剂对醋酸杆菌诱变效果的研究[J].河南科技学院学报(自然科学版),2006,34(9):5-8
[183]徐晓军,宫磊,孟云生等.硫杆菌的化学诱变及对低品位黄铜矿的浸出[J].金属矿山,2004,8:42-44
[184]吴松刚,谢新东,黄建忠等.类产碱假单胞菌耐热碱性脂肪酶的研究[J].微生物学报,1997,37(1):32-39.
[185]Gulati R,Saxena RK,Gupta R,and et al.Parametric optimization of Aspergillus terreus lipase production and its potential in ester synthesis[J].Process Biochem,2000,35:459-464.
[186]Sevgi E,G(o|¨)n(u|¨)l D,Serpil T.Isolation of lipase producing Bacillus sp.From olive mill wastewater and improving its enzyme activity[J].Journal of Hazardous Materials,2007,149:720-724
[187]金波,罗杰,余斐等.产脂肪酶假丝酵母的筛选及其研究[J].华中科技大学学报,2001,29(5):111-113
[188]洪骏,夏黎明.产碱性脂肪酶菌株的筛选和培养[J].过程工程学报,2003,3(5):428-432
[189]高修功,章克昌,曹淑桂.脂肪酶产生菌的选育及产酶条件优化[J].微生物学报,1998,38(4):313-317.
[190]Masaru K,Taichi S,Takeshi M,et al.Biodiesel fuel production from plant oil catalyzed by rhizopus oryzae lipase in a water-containing system without an oganic solvent[J].Journal of Bioscience and Bioengineering,1999,88(6):627-631
[191]Sulaiman AZ,Kishnu VJ,Smita K,et al.The effect of fatty acid concentration and water content on the production of biodiesel by lipase[J].Biochemical Engineering Journal,2006,30:212-217
[192]Noureddini H,Gao X,Philkana R S.Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil[J].Bioresource Technology,2005,96:769-777
[193]Michal H,Pavel J,Jan F,et al.Analytical monitoring of the production of biodiesel by high performance liquid chromatography with various detection methods[J].Journal of Chromatography A,1999,858:13-31
[194]吴苏喜,官春云.菜籽生物柴油合成反应程度的气相色谱法判断[J].中国油脂,2006,31(8):67-69
[195]周位,丁双,闫云君.一种酶法催化合成生物柴油的气相色谱快速测定法[J].当代化工,2007,36(4):469-472
[196]Su Er-Zheng,Zhang Min-Jie,Zhang Jian-Guo,et al.Lipase-catalyzed irreversible transesterification of vegetable oils for fatty acid methyl esters production with dimethyl carbonate as the acyl acceptor[J].Biochemical Engineering Journal,2007,36:167-173
[197]Masaru K,Taichi S,Takeshi M,et al.Biodiesel Fuel Production from Plant Oil Catalyzed by Rhizopus oryzae Lipase in a Water-Containing System without an organic solvent[J].Journal of Bioscience and Bioengineering,1999,88(6):627-631
[198]GB/T 17376-1998,动植物油脂脂肪酸甲酯制备[S].
[199]Toida J,Kondoh K,Fukuzawa M,et al.Purification and characterization of a lipase from Aspergillus oryzae[J].Biosci Biotech Biochem.,1995,59(7):1 199-1 203
[200]Yamaguchi S,Mase T.Purification and characterization of mono-and diacylglycerol lipase isolated from Penicillium camembertii U-150[J].Appl. Microbiol.Biotechnol.,1991,34:800-805
[201]Dlercreutz P.Enzymesin Food Processing[M].NewYork:Academic Press,1993,103-109
[202]Al-Duri B,Robinson E,McNerlan S,et al.Hydrolysisof edible oils by lipase immobilized on hydrophobic support:Effect to internal structure[J].J Am Oil Chem Soc,1995,72:1351-1359
[203]Cao SG,Yang H,Ma L.Enhancing enzymatic properties by the immobilization method[J].Appl Biochem Biotechnol,1996,59:7-14.
[204]Matthijs G,Schacht E.Comparative study of methodologies for obtaining glucosidase immobilized on hydrophilic support[J].Biotechnol Lett,1996,7:647-650
[205]张振乾,官春云.酶催化法生产生物柴油的研究进展[J].中国生物柴油,2009,2:16-20
[206]李堂,周建红,黄朋等.正己烷体系中酶催化菜籽油制备乙酯生物柴油[J].中国油脂,2007,32(12):45-47
[207]刘志强,李堂,黄朋等.叔丁醇体系脂肪酶催化菜籽油制备生物柴油[J].农业工程学报,2007,23(7):174-177
[208]李俐林,杜伟,刘德华等.新型反应介质中脂肪酶催化多种油脂制备生物柴油[J].过程工程学报,2006,6(5):799-803
[209]Neerupma Nawani,Jagdeep Kaur.Studies on lipolytic isoenzymes from a thermophilic Bacillus sp.:Production,purification and biochemical characterization [J].Enzyme and Microbial Technology,2007,40:881-887
[210]徐炜枫,陆兆新,吕凤霞等.固定化脂肪酶Lipozyme TL IM催化菜籽油醇解反应合成生物柴油的工艺条件[J].食品与生物技术学报,2007,26(5):79-83
[211]Volklin DB,Staubli A,Langer R,et al.Enzyme thermo inactivation in anhydrous organic solvents[J].Biotechnol Bioeng,1991,37:843-853.
[212]Zaks A,Rusell A J.Enzymes in organic solvents properties and applications[J].J Biotechnol,1988,8:259-270.
[213]Xu XB.Enzyme production of structured lipase:process reactions and acyl migration[J].INFORM,2000,11:1121-1130.
[214]肖弥彰.高产脂肪酶菌种选育与脂肪酶催化合成生物柴油[D].湖南:长沙,湖南农业大学,2006.
[215]宋玉卿,刘春雷,于殿宇等.酶法催化大豆油脚脂肪酸制备生物柴油的研究[J].食品工业,2008,4:58-61
[216]侯继贤,鲁吉珂,聂开立等.固定化Candida sp.99-125脂肪酶催化大豆油合成脂肪酸乙酯[J].过程工程学报,2008,8(2):355-358
[217]刘志强,李堂,周建红等.分步加醇脂肪酶催化菜籽油制备乙醋生物柴油[J].中国粮油学报,2008,23(2):81-84
[218]王英,周剑忠,黄开红等.固定化脂肪酶催化活性影响因素的改变对生物柴油转化率的影响[J].粮油加工,2007,9:81-84
[219]Laane C,Boeren S,Vos K,et al.Rules for optimization of biocatalysis in organic solvents[J].Biotechnol Bioeng,1987,30:81-87.
[220]Hailing P J.Solvent selection for biocatalysis in mainly organic systems:Prediction of effects on equilibrium position[J].Biotech Bioeng,1990,35:691-701.
[2]Yasuhisa A,Hideki F,Yuji S,et al.Biofuel Production Process by Novel Biocatalysts[J].Journal of Molecular Catalysis B:Enzymatic,2002,17(3-5):111
[3]邓利,谭天伟,王芳.脂肪酶催化合成生物柴油的研究[J].生物工程学报,2003,19(1):97-101
[4]巫淼鑫,邬国英,韩瑛等.6种食用植物油及其生物柴油中脂肪酸成分的比较研究[J].中国油脂,2003,28(12):65-67
[5]杨伟华,许红霞,王延琴等.应用棉子油生产生物柴油的可行性分析[J].中国棉花·棉区了望,2007,34(1):41-42
[6]张欢,孟永彪.用棉籽油制备生物柴油[J].化工进展,2007,26(1):86-89
[7]邬国英,林西平,巫淼鑫等.棉籽油甲酯化联产生物柴油和甘油[J].中国油脂,2003,28(4):70-73
[8]马俊林,郭俊宝,徐一辉等.大豆油制备生物柴油的工艺探索[J].可再生能源,2006,2:34-36
[9]盛梅,郭登峰,张大华等.大豆油制备生物柴油的研究[J].中国油脂,2002,27(1):70-72
[10]何东平,张世宏,齐玉堂等.生物柴油生产技术研究[J].武汉工业大学学报,2003,23(4):52-54
[11]周慧,鲁厚芳,唐盛伟等.麻疯树油制备生物柴油的酯交换工艺研究[J].应用化工,2006,35(4):284-287
[12]韦公远.用米糠油制取生物柴油[J].西部粮油科技,2006,26(1):51-52
[13]王蓉辉,曹祖宾,王亮等.葵花籽油制备生物柴油的研究[J].广州化工,2006,36(1):35-37
[14]G.Antolin,F.V.Tinaut,Y.Briceno,et al.Optimisation of biodiesel production by sunflower oil transesterification[J].Bioresource Technology.2002,83:111-114
[15]刘伟伟,苏有勇,张无敌等.橡胶籽油制备生物柴油的研究[J].中国油脂,2005,30(10):63-66
[16]苏有勇,刘士清,张无敌等.小桐油制备生物柴油的研究[J].新能源及工艺,2006,1:22-26
[17]马传国,司耀彬,侯华锋.皂脚制备生物柴油的研究[J].中国油脂,2006,31(4):59-61
[18]金付强,张建春,杨儒等.大麻籽油合成生物柴油[J].应用化学,2007,24 (1):100-104
[19]Yuji S,Yomi W,Akio S,et al.Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing[J].Journal of Molecular Catatlysis B:Enzymatic,2002,17:133-142
[20]庾乐.非水相体系中酶催化制备生物柴油[D].南宁:广西大学,2006
[21]高静,王芳,谭天伟等.固定化脂肪酶催化废油合成生物柴油[J].化工学报,2005,56(9):1727-1730
[22]陈志锋,吴虹,宗敏华.固定化脂肪酶催化高酸废油脂酯交换生产生物柴油[J].催化学报,2006,27(2):146-150
[23]聂开立,王芳,邓利等.间歇及连续式固定化酶反应生产生物柴油[J].生物加工过程,2005,3(1):58-62
[24]Ana VLP,Enoch YP.Lipase-catalyzed production of Biodiesel fuel from vegetable oils contained in waste activated bleaching earth[J].Process Biochemistry,2003,38:1077-1082
[25]Enoch YP,Masahiro M.Kinetic study of esterification of rapeseed oil contained in waste activated bleaching earth using Candida rugosa lipase in organic solvent system[J].Journal of Molecular Catatlysis B:Enzymatic,2005,37:95-100
[26]Vanessa PL,Enoch YP.Potential application of waste activated bleaching earth on the production of fatty acid alkyl esters using Candida cylindracea lipase in organic solvent system[J].Enzyme and Microbial Technology,2004,34:270-277
[27]Mohamed M S,Uwe T B.Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil[J].Enzyme and Microbial Technology,2003,33:97-103
[28]Wu H,Zong MH,Lou WY.Transesterification of waste oil to biodiesel in solvent free system catalyzed by immobilized lipase[J].Chinese journal of Catalysis,2004,25(11):903-909
[29]John S,Terri D,John B,et al.A Look Back at the U.S.Department of Energy's Aquatic Species Program-Biodiesel from Algae[R].Prepared for:U.S.Department of Energy's Office of Fuels Development,1998
[30]里伟,杜伟,李永红等.生物酶法转化酵母油脂合成生物柴油[J].过程工程学报,2007,7(1):137-140
[31]刘会丽.Novo435酶催化植物油制取生物柴油酯交换反应条件的试验研究[D].郑州:河南农业大学,2006
[32]Veena K,Shweta S,Munishwar N G.Preparation of Biodiesel by Lipase-Catalyzed Transesterification of High Free Fatty Acid Containing Oil from Madhuca indica[J].Energy & Fuels,2007,21:368-372
[33]Kamini NR,Iefuji H.Lipase catalyzed methanolysis of vegetable oils in aqueous medium by Cryptococcus spp.S-2[J].Process Biochemistry,2001,37:405-410
[34]Shweta S,Munishwar N G.Lipase catalyzed preparation of biodiesel from Jatropha oil in a solvent free system[J].Process Biochemistry,2007,42:409-414
[35]Shah S,Sharma S,Gupta M N.Biodiesel Preparation by Lipase-Catalyzed Transesterification of Jatropha Oil[J].Energy & Fuels,2004,18:154-159
[36]倪婉星,张晓鸣.非水相脂肪酶催化合成生物柴油[J].食品与机械,2007,23(1):25-28
[37]陈文伟.乌柏梓油制备生物柴油的研究[D].南昌:南昌大学,2006
[38]田春龙,郭斌,刘春朝.能源植物研究现状和展望[J].生物加工过程,2005,3(1):14-18.
[39]巫淼鑫,邬国英,韩立峰等.食用植物油所制备生物柴油的低温流动性能[J].石油炼制与化工,2005,35(4):57-60.
[40]谢立华,李培武,张文等.油菜作为优势能源作物的发展潜力与展望[J].生物加工过程,2005,3(1):28-31.
[41]王汉中.发展油菜生物柴油的潜力、问题和对策[J].中国油料作物学报,2005,27(2):74-76.
[42]王汉中.实施油菜生物柴油计划建造永不枯竭绿色油田[J].中国农村科技,2005.10:40-41.
[43]张书芬,朱家成,王建平等.选育高油高产油菜品种,大力发展油菜生物柴油[J].河南农业科学,2006,9:52-55.
[44]Breda K.Experimental Investigation of Optimal Timing of the Diesel Engine Injection Pump Using Biodiesel Fuel[J].Energy & Fuels,2006,20:1460-1470.
[45]Leung DYC,Luo Y,Chan T L.Optimization of Exhaust Emissions of a Diesel Engine Fuelled with Biodiesel[J].Energy & Fuels,2006,20:1015-1023.
[46]Tsolakis A.Effects on Particle Size Distribution from the Diesel Engine Operating on RME-Biodiesel with EGR[J].Energy & Fuels,2006,20:1418-1424.
[47]盛梅,李为民,邬国英.生物柴油研究进展[J].中国油脂,2003,28(4):66-70.
[48]李为民,章文峰,邬国英.菜籽油油脚制备生物柴油[J].江苏工业学院学报,2003,15(1):7-10.
[49]Peterson CL,Feldman M,Korus R,et al.Batch type tranesterification process for winter rape oil[J].Applied Engineenng In Agriculture.1991,7(6):711-716.
[50]Barn,Narendra.Process development of rapeseed oil ethyl ester as a dieselfirel substitute[R].Department of Chemical Engineering,University of Idaho,U.S.A.,1991.
[51]Komers K,Machek J,Stloukal R.Biodiesel from rapeseed oil,methanol and KOH.2.Composition of solution of KOH in methanol as reaction partner of oil[J].Eur.J.Lipid Sci.Technol.,2001,103:359-362.
[52]Komers K,Stloukal R,Machek J.Biodiesel from rapeseed oil,methanol and KOH.3.Analysis of composition of actual reaction mixture[J].Eur.J.Lipid Sci.Technol.,2001,103:363-371.
[53]Komers K,Stloukal R,Machek J.Kinetics and mechanism of the KOH-catalyzed methanolysis of rapeseed oil for biodiesel production[J].Eur.J.Lipid Sci.Technol.,2002,104:728-737.
[54]Hemmerlein M,Korte V,Richter H S.Performance,exhaust emission and durability of modem diesel engines running on rapeseed oil[J].SAE Paper,1991,910848.
[55]Recep A,Selim C,H(u|¨)seyin S,et al.The potential of using vegetable oil fuels as fuel for diesel engines[J].Energy Conversion and Management,2001,42:529-538.
[56]Charles L,Peterson,Daryl L,et al.Ethyl ester of rapeseed used as a biodiesel fuel-a case study[J].Biomass and Bioenergy,1996,10:331-336.
[57]邬国英,巫森鑫,林西平等.植物油制备生物柴油[J].江苏石油化工学院学报,2002,12(3):8-11.
[58]盛梅,邬国英,徐鸽等.生物柴油的制备[J].高校化学工程学报,2004,18(2):231-236.
[59]雷猛,许世海,魏小平.菜籽油制备生物柴油及其理化性能的研究[J].后勤工程学院学报,2004,4:59-63.
[60]黄庆德,黄凤洪,郭萍梅.生物柴油生产技术及其开发意义[J].粮食与油脂,2002,9:8-10
[61]Roger A,Korus,Dwight S.Transesterification Process to Manufacture Ethyl Ester of Rape Oil[R].Proceedings of 1st Biomass Conference of the Americas,biodieselgear.com,1992.
[62]张冠杰.利用滴流床反应器制备生物柴油的研究[D].南京:南京工业大学,2004.
[63]徐元浩.生物柴油的实用性研究[D].武汉:武汉理工大学,2005.
[64]张无敌,刘士清,尹芳等.菜籽油酯交换制备生物柴油的工艺研究[J].农业工程学报,2006,22(11):131-134.
[65]李为民,邬国英,徐鸽等.菜籽油制备生物柴油放大实验研究[J].江苏工业学院学报,2005,17(2):9-12.
[66]陈文,王存文,张圣利.碱催化酯交换法制备生物柴油的研究[J].化学与生物工程,2007,24(1):38-40.
[67]蒋大勇,郭和军,张红云等.一种新型菜籽油酯生物柴油的制备[J].可再生 能源,2007,25(1):32-35.
[68]杨凯华.天然产物油脂制备生物柴油新技术的研究[D].北京:中国林业科学研究院,2005.
[69]杨军峰,王忠,孙平等.双低菜籽油制备生物柴油的工艺探索[J].柴油机Diesel Engine,2003,3:43-45.
[70]王鹏.生物柴油的制备及相关研究[D].北京:中国农业大学,2006.
[71]Gryglewicz S.Rapeseed Oil Methyl Esters Preparation Using Heterogeneous Catalysts[J].Bioresour Technol,1999,70(3):249-253.
[72]王广欣,颜姝丽,周重文等.用于生物柴油的钙镁催化剂的制备及其活性评价[J].中国油脂,2005,30(10):66-69.
[73]李为民,郑晓林,徐春明等.固体碱法制备生物柴油及其性能[J].化工学报,2005,56(4):711-716.
[74]Tanja C,Siegfried P,Eckhard W.Biodiesel-Transesterification of Biological Oils with Liquid Catalysts:Thermodynamic Properties of Oil-Methanol-Amine Mixtures[J].Ind.Eng.Chem.Res.2005,44(25):9535-9541.
[75]Kusdiana D,Saka S.Effects of water on biodiesel fuel production by supercritical methanol treatment[J].Bioresour.Technol,2004,91:289-295.
[76]Mittelbach M,Silberholz A,Koncar M.In Novel aspects concerning acid-catalyzed alcoholysis of triglycerides[J].Oils-Fats-Lipids 1995,Proceedings of the 21st World Congress of the International Society for Fats Research,The Hague,1995,1996:497-499.
[77]Samukawa T,Kaieda M,Matsumoto T,et al.Retreament of immobilized Candida antarctica lipase for biodiesel fuel production fiom plant oil[J].J.Biosci.Bioeng,2000,90:180-183.
[78]Watanabe Y,Shimada Y,Sugihara A,et al.Conversion of degummed soybean oil to biodiesel fuel with immobilized Candidn arttarctical lipase[J].J.Molecular Catalysis B:Enzymatic,2002,17:151-155.
[79]Mohamed M,Soumanou,Uwe T.Ornschacer.Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil[J].Enzyme and Microbial Technology,2003,33:97-103.
[80]Watanabe Y,Shimada Y,Sugihara A,et al.Continuous production of biodiesel fuel from vegetable oil using immobilized Candida antarctica lipase[J].J.Am.Oil Chem.Soc.,2000,77:355-360.
[81]徐桂转,刘会丽,张百良等.利用菜籽油酶法生产生物柴油的初步研究[J].农业工程学报,2006,22(8):162-165.
[82]盛梅,郭登峰.固定化酶催化菜籽油合成生物柴油稳定性研究[J].中国油 脂,2005,30(5):68-70.
[83]盛梅,杨文伟,郭登峰.固定化脂肪酶催化合成生物柴油[J].应用化学,2005,22(7):788-791.
[84]蔡志强,邬国英,林西平等.固定化脂酶催化合成生物柴油的研究[J].中国油脂,2004,29(8):29-32.
[85]李俐林,杜伟,刘德华等.乙酸甲酯萃取菜籽油制备生物柴油的研究[J].食品与发酵工业,2006,32(5):5-8.
[86]Uosukainen E,L(a|¨)ms(a|¨) M,Linko Y-Y,et al.Optimization of enzymatic transesterification of rapeseed oil ester using response surface and principal component methodology[J].Enzyme and Microbial Technology,1999,25:236-243.
[87]Linko Y-Y,Tervakangas T,L(a|¨)ms(a|¨) M,et al.Production of trimethylolpropane esters of rapeseed oil fatty acids by immobilized lipase[J].Biotechnology Techniques,1997,11(12):889-892.
[88]Linko Y-Y,Tervakangas T,Huhtala A,et al.Lipase biocatalysis in the production of esters[J].Journal of the American Oil Chemists' Society,1995,72(11):1293-1299.
[89]Lamsa,Merja,Linko,et al.Enzymatic process for preparing a synthetic ester from a vegetable oil[P].US:5 747 434,1998-05-05.
[90]Ayhan D.Biodiesel from vegetable oils via transesterification in supercritical methanol[J].Energy Conversion and Management,2002,43:2349-2356.
[91]Kusdiana D,Saka S.Biodiesel fuel from rapeseed oil as prepared in supercritical methanol[J].Fuel,2001,80(20):225-231.
[92]Hiroaki I,Eiji M,Shusaku H,et al.Thermal Stability of Biodiesel Fuel as Prepared by Supercritical Methanol Process[R].The 2nd Joint International Conference on "Sustainable Energy and Environment(SEE 2006)",2006,11:21-23.
[93]Yuichiro W,Dadan K,Shiro S.Reactivity of triglycerides and fatty acids of rapeseed oil in supercritical alcohols[J].Bioresource Technology,2001,91(3):283-287.
[94]Kusdiana D,Saka S.Kinetics of transesterification in rapeseed oil to biodiesel fuel as treated in supercritical methanol[J].Fuel,2001,80(20):693-698.
[95]赵宗保,华艳艳,刘波.中国如何突破生物柴油产业的原料瓶颈[J].中国生物工程杂志,2005,25(11):1-6.
[96]王涛.中国主要生物质燃料油木本能源植物资源概况与展望[J].科技导报,2005,23(5):12-14.(119)
[97]Gerhard K.Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters[J].Fuel Processing Technology,2005,86:1059-1070.
[98]王小行.新型全细胞催化剂在麻风树生物柴油中的应用[D].成都:四川大学博士研究生毕业论文,2006.
[99]Mamoru I,Chen BX,Eguchi M,et al.Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase[J].Journal of Molecular Catalysis B Enzymatic,2001,16(1):53-58.
[100]Kaieda M,Samukawa T,Kondo A,et al.Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system[J].J Biosci Bioeng,2001,91(1):12-15.
[101](O|¨)znur K,Melek T,Ayse AH.Immobilized Candida Antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium[J].Bioresour Technol.,2002,83:125-129.
[102]余琼,梁运祥.产低温脂肪酶假单胞菌的选育及产酶条件的优化[J].湖北农业科学,2006,45(5):662-665
[103]张金伟,曾润颖.南极产低温脂肪酶菌株Psychrobacter sp.7195的选育、发酵条件及酶学性质研究[J].生物磁学,2006,6(1):6-10
[104]Frederic M.T.,章克昌.从非洲土壤中分离新的产生脂肪酶的细菌[J].无锡轻工大学学报,2000,19(1):18-22
[105]雷晓燕.土壤中产碱性脂肪酶霉菌的筛选[J].沈阳化工学院学报,2006,20(1):9-12
[106]宋欣,魏留梅,刘瑞田等.脂肪酶高产菌株选育和菌种库的建立[J].微生物学通报,2002,29(I):6-10
[107]卢世琦,刘光烨,江跃林等.合成己酸乙酯脂肪酶产生菌的筛选及产酶条件[J].微生物学通报,1994,21(1):23-26
[108]宋欣,曲音波,叶寒青等.具脂肪酶和酯酶活性酵母菌的选育和酶学性质初步研究[J].应用与环境生物学报,1999,5(6):628-631
[109]王乐恒.具脂肪酶和酯酶活性酵母菌的选育及酶学研究[J].青岛建筑工程学院学报,2004,25(2):75-77
[110]Gorokhova IV,Ivanov AE,Zaitsev S.Yu,et al.Study of the interaction and activation of lipase from Pseudomonas fluorescens in surfactant monolayers and precipitates.Russ Chem.Bull,2003,52(4):961-969
[111]吕飒音,胡磊.凝结芽胞杆菌产碱性脂肪酶的条件研究[J].安徽大学学报:自然科学版,2004,28(1):69-72
[112]逢淑召,王群,王智等.荧光假单孢菌Pseudomonas fluorescence 5963产脂肪酶条件的优化[J],生物加工过程,2004,2(1):62-65
[113]Kohno M,Funatsu J,Mikami B,et al.The crystal structure of lipase Ⅱ from Rhizopus niveus at 2.2 A resolution[J].J Biotechem,1996,120:505-510.
[114]Washida M,Takahashi S,Ueda M,et al.Spacer-mediated display of active lipase on the yeast cell surface[J].Appl Microbiol Biotechnol,2001,56:681-686.
[115]Takahashi S,Ueda M,Tanaka A.Function of the prosequence for in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae[J].Appl Microbiol Biotechnol,2001,55:454-462.
[116]徐岩,章克昌,王亚非.微生物脂肪酶在正庚烷中合成短链芳香酯的研究[J].生物工程学报,1998,14(2):214-219
[117]Joerger RD,Haas MJ.Alteration of chain length selectivity of a Rhizopus delemar lipase through site-directed mutagenesis[J].Lipids,1994,29:377-384
[118]Klein RR,King G,Moreau RA,et al.Additive effects of acyl-binding site mutations on the fatty acid selectivity of Rhizopus delemar lipase[J].JAOCS,1997,74(11):1401-1407
[119]侯红漫,吴韬,王运吉.产脂肪酶根霉的诱变及其酶促酯化反应的研究[J].大连轻工业学院学报,1996,15(1):138-141
[120]张冬花,王运吉.高产脂肪酶菌株的诱变筛选[J].生物技术,1996,6(2):20-22
[121]周延,张鹏,孟庆云.用快中子法选育细菌脂肪酶高产菌株[J].微生物学通报,2001,28(2):81-85
[122]黄娟,陈正杰,冯军等.紫外和微波结合诱变选育lipstatin产生菌[J].中国医药工业杂志,2006,37(1):12-14
[123]Jinhong Zhang,Zhibo Hou,Chuanyi Yao,et al.Purilicalion and properlies of lipase from a Bacillus drain for calalylic resolution of(R)-Naproxen.Journal of Molecular Catalvis B:Enzymatic.2002,18:205-210
[124]NR Kamini,T Fujii,T Kurosu,et al.Production,purification and characterization of an extracellular lipase from the yeast,Cryptococcus sp.S-2.Process Biochemistry.2000,36:317-324
[125]N Krieger,MA Taipa,EHM Melo,et al.Purification of a Penicillium citrinum lipase by chromatographic processes.Bioprosses Engineering.1999,20:59-65
[126]闫云君,陈晖,杨江科等.ATPS法分离纯化Aspergillus sp.F044脂肪酶[J].华中科技大学学报(自然科学版),2005,33(10):92-95
[127]辛嘉英,徐毅,李树本等.Candida rugosa脂肪酶同工酶的分离及选择固定化[J].生物化学与生物物理进展,2000,27(5):513-516
[128]邬敏辰,刘为平,许云敏等.碱性脂肪酶的高效液相色谱和质谱分析[J].无锡轻工大学学报,2000,19(5):158-161
[129]吴吟妮,潘剑茹,江贤章等.嗜硫色谱分离纯化碱性脂肪酶及其氨基酸序列测定[J].工业微生物,2004,34(4):158-161
[130]Marttin N,Joha B,Erland H,el al.Lipase immobilized by adsorption[J].Appl Microbiol Biotechnol,1988,28:527-530
[131]Tsuneo Y.Repeated use of lipase immobilized on amphiphilic gel for hydrolysis of a small amount of glycerides in eluded in liquid crude fatty acid[J].Ferment Technol,1982,60(6):517-523
[132]张军,徐家立.固定化假丝酵母1619脂肪酶催化油酸油醇酯的合成[J].生物工程学报,1995,11(4):325-331
[133]刘新喜,彭立凤.蛋壳作载体固定化脂肪酶[J].固原师专学报(自然科学版),2000,1(6):21-24
[134]高阳,谭天伟,聂开立等.大孔树脂固定化脂肪酶及在微水相中催化合成生物柴油的研究[J].生物工程学报,2006,22(1):114-118
[135]陈秀琳,郑毅,王绍钊等.CM-葡聚糖凝胶固定化脂肪酶的研究[J].药物生物技术,1996,6(1):20-23
[136]陈秀琳.CM-纤维素固定化脂肪酶的研究[J].Strait Pharmaceutical Journa,2005,17(1):35-37
[137]徐慧显,李民勤,王道宾等.聚丙烯酸甲酯载体对柱状假丝酵母脂肪酶固定化的研究(Ⅰ)[J].离子交换与吸附,1994,10(4):316-321
[138]谭天伟,王芳,刘天全.用硅藻土固定化脂肪酶及酶法合成单甘油酯[J].中国粮油学报,2000,15(2):29-31
[139]Sailas Benjamin,Ashok Pandey.Enhancement of lipase production during repeated batch culture using immobilized Candida rugosa[J].Process Biochemistry,1997,32(5):437-440
[140]赵庆节,余莹.有机相中大孔吸附树脂固定化酶催化拆分消旋萘普生[J].华东理工大学学报:自然科学版,2001,27(40):423-426.
[141]Wu XY,Jaaskelainen S,Linko YY.An investigation of crude lipases for hydrolysis,esterification and transesterification[J].Enzyme Microb.Technol.1996,19:226-231
[142]Shaw JF,Chang R.C.Lipolytic Activities of a Lipase Immobilization on Six Selected Supporting Materials[J].Biotechnology and Bioengineering,1990,35:132-137
[143]安永磊,唐唯森,高松.酶法催化餐饮废油制备生物柴油的研究[J].吉林大学学报(地球科学版),2006,36增刊:147-150.
[144]徐圆圆,杜伟,刘德华.非水相脂肪酶催化大豆油脂合成生物柴油的研究[J].现代化工,2003,23卷增刊:167-169.
[145]曾淑华,周位,杨江科等.固定化脂肪酶催化大豆油制备生物柴油[J].食品与生物技术学报,2007,26(3):75-79.
[146]王学伟,常杰,吕鹏梅等.固定化脂肪酶催化制备生物柴油[J].石油化工,2005,34(9):855-858.
[147]聂开立,王芳,谭天伟.固定酶法生产生物柴油[J].现代化工,2003,23(9):35-38.(104)
[148]鲁明波,余龙江,薛勇等.生物柴油的酶促合成研究[J].中国油脂,2005,30(11):62-64.
[149]薛勇.微生物脂肪酶及其催化合成生物柴油的研究[D].武汉:华中科技大学硕士研究生毕业论文,2004.
[150]李堂,黄朋,周建红等.正交优化脂肪酶催化菜籽油制备生物柴油[J].湘潭师范学院学报(自然科学版),2007,29(2):22-25.
[151]Noureddini H,Gao X,Philkana RS.Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil[J].Bioresource Technology,2005,96:769-777.
[152]Liu Y,Kondo A,Ohkawa H,et al.Bioconversion Using Immobilized Recombinant Flocculent Yeast Cells Carryinga Fused Enzyme Gene in an "Intelligent" Bioreactor[J].Biochem Eng J,1998,2(4):229-235
[153]Fukuda H.,Kondo A.,Noda H.Biodiesel Fuel Production by Transesterification of Oils[J].J Biosci Bioeng,2001,92(5):405-416
[154]Matsumoto T,Takahashi S,Kaieda M,et al.Yeast Whole-cell Biocatalyst Constructed by Intracellular Overproduction of Rhizopus Oryzaelipase is Applicable to Biodiesel Fuel Production[J].Appl Microbiol Biotechnol,2001,57(4):515-520
[155]Matsumoto T.,Takahashi S.,Ueda M.,et al.Preparation of High Activity Yeast Whole Cell Bioctalysts by Optimization of Intracellular Production of Recombinant Rhizopus Oryzae Lipase[J].Journal of Molecular Catalysis B:Enzymatic,2002,17(3-5):143-149
[156]Kazuhiro B,Shinji H,Keiko N,et al.Repeated use of whole-cell biocatalysts immobilized with biomass support particles for Biodiesel fuel production[J].Journal of Molecular Catatlysis B Enzymatic,2002(17):157-165.
[157]Kazuhiro Ban,Shinji Hama,Keiko Nishizuka,et al.Repeated Use of Whole-cell Biocatalysts Immobilized within Biomass Support Particles for Biodiesel Fuel Production[J].Journal of Molecular Catalysis B:Enzymatic,2002,17(3-5):157-165
[158]Matsumoto T,Fukuda H,Ueda M,et al.Construction of Yeast Strains with High Cell Surface Lipase Activity by Using Novel Display Systems Based on the Flolp Flocculation Functional Domain[J].Applied and Enviromental Microbiology,2002,68(9):4517-4522
[159]Shinji H,Sriappareddy T,Takahiro F,et al.Lipase Localization in Rhizopus oryzae Cells Immobilized within Biomass support Particles for Use as Whole-Cell Biocatalysts in Biodisel-Fuelc Production[J].J.bioscience and bioengineering,2006,101(4):328-333
[160]Ban K,Kaieda M,Matsumoto T,et al.Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles[J].Biochem Eng J,2001,8(1):39-43.
[161]Dossat V,Combes D,Marty A.Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor:influence of the glycerol production[J].Enzyme Microbial Technol.,1999,25:194-200.
[162]Kaili N,Feng X,Fang W,et al.Lipase catalyzed methanolysis to produce biodiesel[J].Journal of Molecular Catalysis B:Enzymatic,2006,43:142-147.
[163]张振乾,官春云.酶催化法合成生物柴油发展现状及对策[J].中国生物柴油,2008,2:16-20
[164]胡爱军,邓捷.超声波辐射对酶法制备生物柴油的影响[J].天津科技大学学报,2007,22(1):29-32.
[165]王学伟.假丝酵母脂肪酶醇解制备生物柴油的研究[D].北京:北京化工大学硕士研究生毕业论文,2006.
[166]陈天祥,吴邦信.菜籽油芥酸提取甲酯化工艺研究[J].贵州化工,1996,(4):12-15
[167]章亭洲,杨立荣,朱自强.脂肪酶改性对其在有机相中催化特性的影响.浙江大学学报(农业与生命科学版).2001,27(3):237-243
[168]En-Shyh Lin,Chee-Chan Wang,Shu-Chiao Sung.Cultivating conditions influence lipase production by the edible Basidiomycete Antrodia cinnamomea in submerged culture[J].Enzyme and Microbial Technology,2006,39:98-102
[169]Shinji H,Hideki Y,Masaru K,et al.Effect of fatty acid membrane composition on whole-cell biocatalysts for biodiesel-fuel production[J].Biochemical Engineering Journal,2004,21:155-160
[170]MMD Maia,A Heasley,MM Camargo de Morais,et al.Effect of culture conditions on lipase production by Fusarium solani in batch fermentation[J].Bioresource Technology,2001,76:23-27
[171]JA Rodriguez,JC Mateos,J Nungaray,et al.Improving lipase production by nutrient source modification using Rhizopus homothallicus cultured in solid state fermentation[J].Process Biochemistry,2006,41:2264-2269
[172]Yao-Qiang He,Tian-Wei Tan.Use of response surface methodology to optimize culture medium for production of lipase with Candida sp.99-125[J].Journal of Molecular Catalysis B:Enzymatic,2006,43:9-14
[173]Tsuchiya K,Tada S,Gomi K,et al.High level expression of the synthetic human lysozyme gene in Aspergillus oryzae[J].Appl Micrdbiol Biotechnol,1992,38:109-114.
[174]Zheng XF,Kobayashi Y,Takeuchi M.Construction of a low-serinetype -carboxypeptidase-producing mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion[J].Appl Microbiol Biotechnol,1998,49:39-44.
[175]JL Mau,JY Li.Effect of 10-oxo-trans-8-decenoic acid on the growth and enzyme activities of Aspergillus niger and A.oryzae[J].Fungal Science.2002,17(4):65-76.
[176]HP Chen,KF Hsiao,SH Wu.Regioselectivity Enhancement by Partially Purified Lipase from Aspergillus Niger[J].Biotechnol.Lett.1995,17:305-308.
[177]Chen Jyh-Ping,Mcgill SD.Enzymatic hydrolysis of triglycerides by Rhizopus delemar immobilized on biomass support particles[J].Food Biotechnol,1992,6(1):1-18
[178]Elibol M,Ozer D.Lipase production by immobilized Rhizopus arrhizus[J].Process Biochem,2000,36:219-223
[179]Salleh AB,Musani R,Basri M,et al.Extra-and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production[J].Can J Microbiol.1993.39:978-981
[180]邬敏辰,孙崇荣,乌显章等.平板扩散法粗略确定碱性脂肪酶的活性[J].无锡轻工大学学报,2000,19(2):168-172
[181]Abramic M,Lescic I,Korica T,et al.Purification and properties of extracellular lipase from Streptomyces rimosus[J].Enzyme and Microb Technol,1999,25:522-529
[182]王树宁,高晗,孙俊良等.不同诱变剂对醋酸杆菌诱变效果的研究[J].河南科技学院学报(自然科学版),2006,34(9):5-8
[183]徐晓军,宫磊,孟云生等.硫杆菌的化学诱变及对低品位黄铜矿的浸出[J].金属矿山,2004,8:42-44
[184]吴松刚,谢新东,黄建忠等.类产碱假单胞菌耐热碱性脂肪酶的研究[J].微生物学报,1997,37(1):32-39.
[185]Gulati R,Saxena RK,Gupta R,and et al.Parametric optimization of Aspergillus terreus lipase production and its potential in ester synthesis[J].Process Biochem,2000,35:459-464.
[186]Sevgi E,G(o|¨)n(u|¨)l D,Serpil T.Isolation of lipase producing Bacillus sp.From olive mill wastewater and improving its enzyme activity[J].Journal of Hazardous Materials,2007,149:720-724
[187]金波,罗杰,余斐等.产脂肪酶假丝酵母的筛选及其研究[J].华中科技大学学报,2001,29(5):111-113
[188]洪骏,夏黎明.产碱性脂肪酶菌株的筛选和培养[J].过程工程学报,2003,3(5):428-432
[189]高修功,章克昌,曹淑桂.脂肪酶产生菌的选育及产酶条件优化[J].微生物学报,1998,38(4):313-317.
[190]Masaru K,Taichi S,Takeshi M,et al.Biodiesel fuel production from plant oil catalyzed by rhizopus oryzae lipase in a water-containing system without an oganic solvent[J].Journal of Bioscience and Bioengineering,1999,88(6):627-631
[191]Sulaiman AZ,Kishnu VJ,Smita K,et al.The effect of fatty acid concentration and water content on the production of biodiesel by lipase[J].Biochemical Engineering Journal,2006,30:212-217
[192]Noureddini H,Gao X,Philkana R S.Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil[J].Bioresource Technology,2005,96:769-777
[193]Michal H,Pavel J,Jan F,et al.Analytical monitoring of the production of biodiesel by high performance liquid chromatography with various detection methods[J].Journal of Chromatography A,1999,858:13-31
[194]吴苏喜,官春云.菜籽生物柴油合成反应程度的气相色谱法判断[J].中国油脂,2006,31(8):67-69
[195]周位,丁双,闫云君.一种酶法催化合成生物柴油的气相色谱快速测定法[J].当代化工,2007,36(4):469-472
[196]Su Er-Zheng,Zhang Min-Jie,Zhang Jian-Guo,et al.Lipase-catalyzed irreversible transesterification of vegetable oils for fatty acid methyl esters production with dimethyl carbonate as the acyl acceptor[J].Biochemical Engineering Journal,2007,36:167-173
[197]Masaru K,Taichi S,Takeshi M,et al.Biodiesel Fuel Production from Plant Oil Catalyzed by Rhizopus oryzae Lipase in a Water-Containing System without an organic solvent[J].Journal of Bioscience and Bioengineering,1999,88(6):627-631
[198]GB/T 17376-1998,动植物油脂脂肪酸甲酯制备[S].
[199]Toida J,Kondoh K,Fukuzawa M,et al.Purification and characterization of a lipase from Aspergillus oryzae[J].Biosci Biotech Biochem.,1995,59(7):1 199-1 203
[200]Yamaguchi S,Mase T.Purification and characterization of mono-and diacylglycerol lipase isolated from Penicillium camembertii U-150[J].Appl. Microbiol.Biotechnol.,1991,34:800-805
[201]Dlercreutz P.Enzymesin Food Processing[M].NewYork:Academic Press,1993,103-109
[202]Al-Duri B,Robinson E,McNerlan S,et al.Hydrolysisof edible oils by lipase immobilized on hydrophobic support:Effect to internal structure[J].J Am Oil Chem Soc,1995,72:1351-1359
[203]Cao SG,Yang H,Ma L.Enhancing enzymatic properties by the immobilization method[J].Appl Biochem Biotechnol,1996,59:7-14.
[204]Matthijs G,Schacht E.Comparative study of methodologies for obtaining glucosidase immobilized on hydrophilic support[J].Biotechnol Lett,1996,7:647-650
[205]张振乾,官春云.酶催化法生产生物柴油的研究进展[J].中国生物柴油,2009,2:16-20
[206]李堂,周建红,黄朋等.正己烷体系中酶催化菜籽油制备乙酯生物柴油[J].中国油脂,2007,32(12):45-47
[207]刘志强,李堂,黄朋等.叔丁醇体系脂肪酶催化菜籽油制备生物柴油[J].农业工程学报,2007,23(7):174-177
[208]李俐林,杜伟,刘德华等.新型反应介质中脂肪酶催化多种油脂制备生物柴油[J].过程工程学报,2006,6(5):799-803
[209]Neerupma Nawani,Jagdeep Kaur.Studies on lipolytic isoenzymes from a thermophilic Bacillus sp.:Production,purification and biochemical characterization [J].Enzyme and Microbial Technology,2007,40:881-887
[210]徐炜枫,陆兆新,吕凤霞等.固定化脂肪酶Lipozyme TL IM催化菜籽油醇解反应合成生物柴油的工艺条件[J].食品与生物技术学报,2007,26(5):79-83
[211]Volklin DB,Staubli A,Langer R,et al.Enzyme thermo inactivation in anhydrous organic solvents[J].Biotechnol Bioeng,1991,37:843-853.
[212]Zaks A,Rusell A J.Enzymes in organic solvents properties and applications[J].J Biotechnol,1988,8:259-270.
[213]Xu XB.Enzyme production of structured lipase:process reactions and acyl migration[J].INFORM,2000,11:1121-1130.
[214]肖弥彰.高产脂肪酶菌种选育与脂肪酶催化合成生物柴油[D].湖南:长沙,湖南农业大学,2006.
[215]宋玉卿,刘春雷,于殿宇等.酶法催化大豆油脚脂肪酸制备生物柴油的研究[J].食品工业,2008,4:58-61
[216]侯继贤,鲁吉珂,聂开立等.固定化Candida sp.99-125脂肪酶催化大豆油合成脂肪酸乙酯[J].过程工程学报,2008,8(2):355-358
[217]刘志强,李堂,周建红等.分步加醇脂肪酶催化菜籽油制备乙醋生物柴油[J].中国粮油学报,2008,23(2):81-84
[218]王英,周剑忠,黄开红等.固定化脂肪酶催化活性影响因素的改变对生物柴油转化率的影响[J].粮油加工,2007,9:81-84
[219]Laane C,Boeren S,Vos K,et al.Rules for optimization of biocatalysis in organic solvents[J].Biotechnol Bioeng,1987,30:81-87.
[220]Hailing P J.Solvent selection for biocatalysis in mainly organic systems:Prediction of effects on equilibrium position[J].Biotech Bioeng,1990,35:691-701.