用cDNA-AFLP指纹技术分离MeJA诱导的人参皂苷生物合成相关基因
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摘要
人参皂苷具有广泛的药理活性,深入了解人参皂苷生物合成途径及其调控的分子遗传学基础,从而在分子水平实现皂苷生物合成的人工调控,将是发展生物技术大量生产人参皂苷的重要前提。甲基茉莉酮酯(MeJA)为一种外源化学诱导子能显著提高人参细胞中皂苷的产量,而皂苷含量和组成的变化又取决于皂苷合酶及其转录调节子在细胞中的表达水平。为阐明人参皂苷生物合成途径及分子调控机制,本研究以生长于吉林省长白山区的四年生人参根为外植体材料,成功诱导出人参根愈伤组织,后经液体振荡培养后成功建立了人参悬浮细胞系,选择MeJA诱导人参皂苷合成的200μM最适剂量,处理人参悬浮细胞24 h,通过改进RNA提取方法获得高质量的总RNA,经纯化mRNA,逆转录成cDNA,利用cDNA-AFLP指纹技术,获得MeJA诱导后与对照组比较差异表达的52条cDNA片段,经DNA测序获得了28个可能参与人参皂苷生物合成有关的cDNA片段序列,生物信息学分析,结果显示其中16条与GeneBank中已知功能基因同源,主要涉及参与次生代谢物转运的ABC转运蛋白基因,参与次生代谢物合成的ATP硫酸腺苷基转移酶、二氢硫辛酰胺S-乙酰基转移酶、黄体酮5β-还原酶及裂合酶基因;参与转录调控的锌指蛋白基因,参与植物防御反应的HSP60、富羟脯氨酸糖蛋白及谷胱甘肽S-转移酶基因,参与细胞信号传导的磷酸二酯酶基因。
本研究采用cDNA-AFLP指纹技术,筛选出28条MJA诱导人参皂苷合成相关基因的cDNA片段,为进一步克隆其基因全长,阐明其在人参皂苷生物合成中的功能提供了重要的实验依据。
Ginsenosides have various pharmacological activities with a wide spectra of usefulness, to dissect the ginsenoside biosynthetic pathways and their molecular genetic mechanisms in depth became important premise for the development of in dustrial manufacturing biotechs.Studies have shown that MeJA is a chemical elicitor,which is able to improve the ginsenoside production.In fact, this suggested that the differential temporal expression of ginsenoside biosynthase and/or transcriptional regulator genes might play important roles in ginsenoside biosynthesis.In this experiment,we collected ginseng from Changbai Mountain, Jilin province, used it as the explant material.Ginseng callus cultures have been successfully induced and stably subcultured from one generation to another , and are maintained in a modified MS medium.Ginseng suspension cell cultures have also been successfully established. In this study,cDNA-AFLP analysis was employed to identify genes that exhibited a modulated expression following 200μM MeJA treatment in Panax ginseng grown in suspension culture.A total of twenty-eight unique TDFs was found as MeJA responsive. sixteen of them showed significant homology to genes with known or putative function,six TDFs were homologous to uncharacterized genes, while six TDFs did not show significant matches. Twenty-eight genes of known or putative function were transcriptional factors, expression regulators, and stress responding and transport facilitation genes, as well as genes involved in cellular metabolism and organization and the photosynthetic process, suggesting that a multitude of processes are implicated in MeJA response.
本研究采用cDNA-AFLP指纹技术,筛选出28条MJA诱导人参皂苷合成相关基因的cDNA片段,为进一步克隆其基因全长,阐明其在人参皂苷生物合成中的功能提供了重要的实验依据。
Ginsenosides have various pharmacological activities with a wide spectra of usefulness, to dissect the ginsenoside biosynthetic pathways and their molecular genetic mechanisms in depth became important premise for the development of in dustrial manufacturing biotechs.Studies have shown that MeJA is a chemical elicitor,which is able to improve the ginsenoside production.In fact, this suggested that the differential temporal expression of ginsenoside biosynthase and/or transcriptional regulator genes might play important roles in ginsenoside biosynthesis.In this experiment,we collected ginseng from Changbai Mountain, Jilin province, used it as the explant material.Ginseng callus cultures have been successfully induced and stably subcultured from one generation to another , and are maintained in a modified MS medium.Ginseng suspension cell cultures have also been successfully established. In this study,cDNA-AFLP analysis was employed to identify genes that exhibited a modulated expression following 200μM MeJA treatment in Panax ginseng grown in suspension culture.A total of twenty-eight unique TDFs was found as MeJA responsive. sixteen of them showed significant homology to genes with known or putative function,six TDFs were homologous to uncharacterized genes, while six TDFs did not show significant matches. Twenty-eight genes of known or putative function were transcriptional factors, expression regulators, and stress responding and transport facilitation genes, as well as genes involved in cellular metabolism and organization and the photosynthetic process, suggesting that a multitude of processes are implicated in MeJA response.
引文
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