银线草抗肿瘤有效部位化学成分及指纹图谱研究
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摘要
银线草为金粟兰科植物银线草(Chloranthus japonicus Sieb)的植物名。银线草药用历史悠久,生理活性较强,民间应用十分广泛。祛湿散寒、活血止痛、散瘀解毒,主治风寒咳嗽、风湿痛、闭经,跌打损伤、瘀血肿痛、核咳血、肺结淋巴腺炎、神经衰弱、毒蛇咬伤、疟疾、疮痈等。银线草分布于我国黑龙江、吉林、河北、山西、山东、峡西、甘肃等省。植物资源极为丰富。但长期以来,对该植物几乎没有进行有效的开发和利用。文献报道银线草有抗肿瘤作用,为找到其抗肿瘤有效成分并对药材质量加以控制,本文对银线草抗肿瘤有效部位进行化学成分和指纹图的研究。
本文对银线草抗肿瘤的有效部位进行了系统的提取分离和结构鉴定研究。通过硅胶、ODS、Sephadex LH-20等柱色谱和HPLC等现代提取分离方法和技术,分离出10个化合物,采用~1H-NMR、~(13)C-NMR、~1H-~1HCOSY、~1H-~(13)CCOSY、HMBC和NOESY等1D及2D-NMR波谱学测试技术,并结合ESI-MS,HR-ESI-MS等质谱,鉴定了其中6个化合物,结构分别为1个木脂素类化合物(cleomiscosin C-4-O-β-glucopyraNoside),2个倍半萜类化合物(pisumioNoside and 1α-hydroxyeudesman-4(15),7(11)-dien-8α-12-olide-1-O-glucopyraNoside),2个皂苷类化合物(CaulosideD and LenticinD)和1个香豆素类化合物(kimmin-7-O-glucopyrano-side)。
通过对银线草指纹图谱的研究,确立了适宜的色谱测定条件,通过对精密度、稳定性、重现性等方法学考察,表明所建立的银线草指纹图谱测定方法符合中药指纹图谱的技术要求,可用于银线草指纹图谱的测定。对不同产地和来源的银线草药材进行了指纹图谱测定,确定了20个共有峰,对银草药材指纹图谱进行了相似度比较,对相似度评价指标夹角余弦和相关系数进行了测算,结果显示不同产地和来源的银线草药材相似度良好。
本文通过对银线草抗肿瘤有效部位进行系统深入的化学成分和指纹图谱的研究,为今后深入研制临床急需的抗肿瘤新药提供了坚实的研究基础和科学依据。本研究填补国内外对银线草的抗肿瘤化学成分和指纹图谱研究领域的空白,不仅具有重要的理论和学术价值,也具有非常广阔的应用前景。
Chloranthus japonicus Sieb is a kind of perennial herbaceous plant, which belongs to the family of Chloranthaceae.Chloranthus japonicus Sied has been widely used to treat cold rheum,injuries from falls,fracture, rheumatism ache,pulmonary tuberculosis,neurasthenic,impaludism,etc.in the civilian society of China.It mainly grows in shady places,including hillside,valley,tussock,etc.Chloranthus japonicus Sieb has comprehensively distributed in many places in China,including Heilongjiang,Jilin,Hebei, Shanxi,Shandong,Shanxi,Gansu,etc.Chloranthus japonicus Sieb is very rich in the plant resources in China.However,for the long time,this plant has Not been exploited and utilized availably by people.
For better illumination medical substance basis on treating cancer,we have studies on chemical constituents of 50%ethaNol elution section of macroporous absorption resin for Chloranthus japonicus Sieb by silica gel, ODS,Sephadex LH-20 column chromatography and HPLC preparation chromatography.As a result,chemical structures of 6 compounds were fully determined by 1D and 2D NMR such as ~1H-NMR,~(13)C-NMR,DEPT, ~1H-~1HCOSY,HSQC and HMBC,etc,combined with ESI-MS and HR-ESI-MS, UV,IR and chemical methods.The compounds are one lignans (cleomiscosin C-4-O-β-glucopyraNoside),two sesquiterpences(pisumio No side and 1α-hydroxyeudesman-4(15),7(11)-dien-8α-12-olide-l-O- glucopyra Noside),two saponin compounds(CaulosideD,LenticinD)and one coumarins (kimmin-7-O-glucopyra Noside).
Proper method for the determination of HPLC fingerprint spectrum of Chloranthus japonicus Sieb was established and up to the technical demands for TCM fingerprint spectrums.HPLC fingerprint spectrums of different places and sources of Chloranthus japonicus Sieb were determined and 20 common fingerprint peaks were asertained.Similarities of the fingerprint spectrums were compared and showed that their compenents were similar.
This dissertation elucidated the anti-tumor material basis of Chloranthus japonicus Sieb by chemical and Fingerprint Spectrums research. It will provide important scientific foundation for further research and the development of new drugs and promote the exploitation of this abundant natural medicinal resource.
本文对银线草抗肿瘤的有效部位进行了系统的提取分离和结构鉴定研究。通过硅胶、ODS、Sephadex LH-20等柱色谱和HPLC等现代提取分离方法和技术,分离出10个化合物,采用~1H-NMR、~(13)C-NMR、~1H-~1HCOSY、~1H-~(13)CCOSY、HMBC和NOESY等1D及2D-NMR波谱学测试技术,并结合ESI-MS,HR-ESI-MS等质谱,鉴定了其中6个化合物,结构分别为1个木脂素类化合物(cleomiscosin C-4-O-β-glucopyraNoside),2个倍半萜类化合物(pisumioNoside and 1α-hydroxyeudesman-4(15),7(11)-dien-8α-12-olide-1-O-glucopyraNoside),2个皂苷类化合物(CaulosideD and LenticinD)和1个香豆素类化合物(kimmin-7-O-glucopyrano-side)。
通过对银线草指纹图谱的研究,确立了适宜的色谱测定条件,通过对精密度、稳定性、重现性等方法学考察,表明所建立的银线草指纹图谱测定方法符合中药指纹图谱的技术要求,可用于银线草指纹图谱的测定。对不同产地和来源的银线草药材进行了指纹图谱测定,确定了20个共有峰,对银草药材指纹图谱进行了相似度比较,对相似度评价指标夹角余弦和相关系数进行了测算,结果显示不同产地和来源的银线草药材相似度良好。
本文通过对银线草抗肿瘤有效部位进行系统深入的化学成分和指纹图谱的研究,为今后深入研制临床急需的抗肿瘤新药提供了坚实的研究基础和科学依据。本研究填补国内外对银线草的抗肿瘤化学成分和指纹图谱研究领域的空白,不仅具有重要的理论和学术价值,也具有非常广阔的应用前景。
Chloranthus japonicus Sieb is a kind of perennial herbaceous plant, which belongs to the family of Chloranthaceae.Chloranthus japonicus Sied has been widely used to treat cold rheum,injuries from falls,fracture, rheumatism ache,pulmonary tuberculosis,neurasthenic,impaludism,etc.in the civilian society of China.It mainly grows in shady places,including hillside,valley,tussock,etc.Chloranthus japonicus Sieb has comprehensively distributed in many places in China,including Heilongjiang,Jilin,Hebei, Shanxi,Shandong,Shanxi,Gansu,etc.Chloranthus japonicus Sieb is very rich in the plant resources in China.However,for the long time,this plant has Not been exploited and utilized availably by people.
For better illumination medical substance basis on treating cancer,we have studies on chemical constituents of 50%ethaNol elution section of macroporous absorption resin for Chloranthus japonicus Sieb by silica gel, ODS,Sephadex LH-20 column chromatography and HPLC preparation chromatography.As a result,chemical structures of 6 compounds were fully determined by 1D and 2D NMR such as ~1H-NMR,~(13)C-NMR,DEPT, ~1H-~1HCOSY,HSQC and HMBC,etc,combined with ESI-MS and HR-ESI-MS, UV,IR and chemical methods.The compounds are one lignans (cleomiscosin C-4-O-β-glucopyraNoside),two sesquiterpences(pisumio No side and 1α-hydroxyeudesman-4(15),7(11)-dien-8α-12-olide-l-O- glucopyra Noside),two saponin compounds(CaulosideD,LenticinD)and one coumarins (kimmin-7-O-glucopyra Noside).
Proper method for the determination of HPLC fingerprint spectrum of Chloranthus japonicus Sieb was established and up to the technical demands for TCM fingerprint spectrums.HPLC fingerprint spectrums of different places and sources of Chloranthus japonicus Sieb were determined and 20 common fingerprint peaks were asertained.Similarities of the fingerprint spectrums were compared and showed that their compenents were similar.
This dissertation elucidated the anti-tumor material basis of Chloranthus japonicus Sieb by chemical and Fingerprint Spectrums research. It will provide important scientific foundation for further research and the development of new drugs and promote the exploitation of this abundant natural medicinal resource.
引文
[1]Fertay J,Bray F,Pisani P,et al.GLOBOCAN2002:Cancer incidence,mortality and prevalence worldwide[M].Version 2.0.IARC CancerBaseNo 5.Lyon:IARC,2004.
[2]Wang HK,Morris Natschke SL,Lee KH.Recent advances in the discovery and development of topoisomerase inhibitors as antitumoragents.Med Res Rev,1997;17(4):367-425
[3]江苏新医学院.中药大辞典.下册,上海人民出版社,1977:4479
[4]黄泰康,丁志遵,赵守训,等.现代本草纲目.下卷,中国中医药科技出版社,2000:2574-2575
[5]冉先德.中华药海.下卷,哈尔滨出版社.1993:1736-1737
[6]张惠源,张志英,岳俊三,等.中国中药资源志要.科学出版社,1994:392
[7]江苏新医学院.中华本草.3卷,上海科学技术出版社,2055-2056
[8]中国药科大学.中药辞海.第三卷,中国医药科技出版社,1997:387
[9]邱德文,吴家荣,等.本草纲目彩色药图.草部,第13卷,贵州科技出版社,1988:155
[10]中国植物志 中国科学院植物研究所 77
[11]王天松.植物学报,1987,29(2):184
[12]黄卫东.分析化学,1998,26(9):1081
[13]《中华本草》编委会.中华本草.第三卷.上海:上海科学出版社,1999:449
[14]De Mayo P,et al.Tetrahedron,1965,21:619
[15]Kawabata Jun,et al.CA.1984.101:3909
[16]T.Takemoto,M.Uchida,K.Koike,Y.Hoshina,G.KusaNo,Chem.Pharm.Bull.1975,23,1161.
[17]高诚伟,等.高等学校化学学报,1987,8(2):141
[18]Masaaki Uchida,Yutaka Koike,Genjiro KusaNo,etal..Chem Pharm.Bull,1980,28(1),92-102
[19]Jun Kawabata,Junya Mizutani.ShizukaNolides D,E and F,Agric Biol Chem.1989,53(1),203-207
[20]徐英含,陈惜秋.及己、川乌与莽草中毒.中华内科杂志,人民卫生出版社出版,1958:6(2):74
[21]谢培山.中药制剂色谱指纹图谱(图像)鉴别.中成药,2000,22(6):391-395
[22]李克,王曙东.中草药,2003,34(11):附8-附10
[23]王夏炎.中草药,2004,35(7):附6-附9
[24]Xie P S,Yah Y Z.HPTLC fingerprint identification of ginseng[J].J High Resol Chromatogr Chromatogr Comm,1987,10(11):607-610
[25]祝晨,林朝展.黄柏药材薄层色谱特征鉴别研究.广东药学,2004,14(1):8-9
[26]洪筱坤,王志华.柴胡属19种植物挥发油的GC相对保留值的指纹图谱分析.药学学报,1988,23(1):839-845
[27]王智华,洪筱坤,包雪声.进口檀香木的GC比较分析.中国中药杂志,1991,16(1):40-43
[28]钱浩泉,李彩君,谢培山.高良姜及其近缘植物挥发油成分的气相色谱指纹图谱研究.中药新药与临床药理,2001,12(3):179-182
[29]丁平,曾元儿,何智健.不同产地阳春砂挥发油气相色谱指纹图谱研究.中国药学杂志,2004,39(6):418-420
[30]洪筱坤,王志华,李旭.HPLC-相对保留值指纹谱法鉴别大黄.中国中药杂志,1993,18(11):650-652
[31]张聪,王志华,金德庄.中国红参与高丽红参的指纹谱(HPLC-FPS)比较研究.中成药,2001,23(3):160-163
[32]曾志,杨东晖,宋力飞等.高效液相色谱指纹图谱应用于板监根的鉴定.分析化学,2002,30(7):849-852
[33]王雁,毕开顺.三七HPLC指纹图谱的建立.中国中药杂志,2003,28(4):316-320
[34]刘艳娥,吴玫涵,吴玉田等.金银花药材高效液相指纹图谱研究.解放军药学学报,2003,19(3):161-164
[35]陈士林,陈四保,徐宏喜等.葛根高效液相指纹图谱的研究.中草药,2003,34(7):661-663
[36]曾志,杨东晖,陶敬奇等.高效液相笆谱指纹图谱在中药芦根上的应用.分析化学,2004,32(8):1035-1038
[37]金永新,要林青,封士兰等.乌拉尔甘草高效液相指纹图谱的研究.中国现代应用药学,2004,21(2):93-98
[38]严启新.鸡血藤高效液相色谱指纹图谱研究.中草药,2004,35(5):556-559
[39]曹进,徐燕,张永知等.栀子药材的指纹图谱整体性分析.分析化学,2004,32(7):875-878
[40]Hasler A,Sticher O.Identification and determination of the flavoNoids from Ginkgo biloba by high-performance liquid chromatography[J].J Chromatogr,1992,605:41-48
[41]张皓冰,陶奕,洪筱坤等.HPLC-MS在麝香酮分析中的应用.中草药,2002,33(11):970-973
[42]夏泉,李绍平,黄赵刚等.三七总皂苷注射液HPLC指纹图谱的比较分析.中成药2004,26(5):345-348
[43]王钢力,戴忠,鲁静等.11种卷柏属药用植物HPCE指纹图谱的研究.中成药,2002,24(7):489-491
[44]曹敏,季一兵,陈玉英.栀子药材毛细管电泳指纹图谱的研究。中国药科大学学报,2002,33(4):284-286
[45]孙沂,隋因,郭涛等.不同产地红花药材的高效毛细管电泳指纹图谱研究.中国药学杂志,2003,38(3):176-178
[46]魏英勤,袁久荣,闫滨.黄连的毛细管电泳特征指纹图谱研究.中草药,2003,34(6):563-565
[47]宋贤丽,郭宝林,潘映红等.6种决明属药用植物种子毛细管电泳法鉴别.中国中药杂志,2003,28(6):491-496
[48]季一兵,郑朝华,陈玉英.丹参的毛细管电泳指纹图谱研究.中成药,2003,25(12):953-955
[49]孙国徉,万月生,孙毓庆.射干的毛细管电泳指纹图谱研究.色谱,2004,22(3):206-209
[50]周红涛,胡世林,冯学峰等.不同产地赤芍的FTIR指纹图谱对比分析.中草药,2002,33(9):834-837
[51]程存旧,孙跃宗.延胡索及其伪品的FTIR直接鉴别.中草药,2002,33(12):1125-1128
[52]陈畅,陈望化,李彦等.蒙山九州虫草与冬虫夏草红外光谱的比较.山东中医杂志,2004,23(2):109-110
[53]袁旭江,林励,李向明.毛橘红与光橘红的紫外光谱和薄层色谱指纹图谱研究.广州中医药大学学报,2003,20(4):305-307
[54]秦海林,赵天增.核磁共振氢谱鉴别植物中药的研究.药学学报,1999,34(1):58-62
[55]秦海林,张建新,王峥涛等.环草石斛的~H-NMR指纹图谱解析.中国中药杂志,2002,27(12):919-923
[56]秦海林,赵天增,袁卫梅等.虎杖、何首乌和掌叶大黄、唐古特大黄的~1H-NMR 指图谱解析.药学学报,1999,34(11):288-834
[57]秦海林,赵天增,尚玉俊等.苦皮藤根皮的~1H-NMR指纹图谱解析.药学学报,2001,36(6):462-466
[58]仇熙,贾晓斌,陈廉等.复方人参注射液核磁共振指纹图谱研究.中成药,2004,26(3):173-174
[59]吕扬,郑启泰,吴楠.中药材X-射线衍射图谱研究.药学学报,1997,32(3):193-198
[60]吕扬,郑启泰,章菽.中药材山奈的X衍射Fourier图分析.药学学报,1998,33(8):587-590
[61]吴云山,王树春,李兰燕等.中药材白芍的X射线衍射Fourier谱鉴定.中草药,2002,33(10):939-941
[62]刘小平,李惠,徐海星等.白花蛇舌草的X射线衍射Fourier指纹图谱鉴定.中药材,2003,26(7):488-490
[63]李晓波,伏见裕利.基于PCR技术的中药DNA分子标记鉴别.中草药,2002,33(8):760-762
[64]肖小何,刘峰群,袁海龙等.中药DNA分子标识鉴定研究进展.中草药,2000,31(8):561-564
[65]梁之桃,秦民建,王峥涛等.柴胡属5种植物RAPD分析与分类鉴定.中草药,2002,33(12):1117-1119
[66]杨美华,张大明,刘健全等.正品和伪品大黄的RAPD指纹图谱鉴定研究.中草药,2003,34(6):557-560
[67]刘文生,朱建明,何斌等.中药材厚朴的随机扩增多态性DNA指纹图谱研究.中药材,2004,27(3):164-169
[68]王夏炎.现代分析技术在中药指纹图谱研究中的应用.中草药,2004,35(7):附6-附9
[69]罗国安,王义明,曹进.多维多息特征谱及其应用.中成药,2000,22(6):395-397
[70]WHO.Guidelines for the Assesment of herbal Medicines.1996
[71]FAD Guidance for Industry-Botanical Drug Products(Draft Guidance).Ⅷ,B.2e;3e;August,2000
[72]谢培山.中药制剂色谱指纹图谱(图像)鉴别.中成药,2000,22(6):391
[73]Rudolf Bauer.Quality Criteria and Phytopharmaceutical:Can Accept Drug Standards be achieved[J].Drug information Jornal,1998,32:101
[74]EMEA.Note for Guidance on Quality of Herbal Medicinal Products.1998
[75]Young-Won and Jinwoong Kim.Tetrahedon letters 2004,45,339-341
[76]Murakami,Toshiyuki;KohNo,Kentarou;NiNomiya,Kiyofumi,etal.Chem Pharm.Bull 2001,49(8),1003-1008.
[77]Haruhisa Kizu,Shingo Kitayama,Fukio Nakatani et al.Chem Pharm Bull.1985,33(8):3324-3329.
[78]E.A.Sobolev,V.I.Grishkovets,A.S.Shashkov et al.Chemistry of Natural compounds.2000,36(5):538-539.
[79]赵萍萍.药学学报,1987,22(1):70-74
[80]李创军,张东明,罗永明 宽叶金粟兰化学成分的研究2005 40(6):525-528.
[81]赵萍萍.药学学报,1987,22(1):70-74
[82]王玺,王文宁,张克荣等.中药HPLC指纹图谱相似性研究的探讨.沈阳药科大学学报,2003,20(5):360-362
[2]Wang HK,Morris Natschke SL,Lee KH.Recent advances in the discovery and development of topoisomerase inhibitors as antitumoragents.Med Res Rev,1997;17(4):367-425
[3]江苏新医学院.中药大辞典.下册,上海人民出版社,1977:4479
[4]黄泰康,丁志遵,赵守训,等.现代本草纲目.下卷,中国中医药科技出版社,2000:2574-2575
[5]冉先德.中华药海.下卷,哈尔滨出版社.1993:1736-1737
[6]张惠源,张志英,岳俊三,等.中国中药资源志要.科学出版社,1994:392
[7]江苏新医学院.中华本草.3卷,上海科学技术出版社,2055-2056
[8]中国药科大学.中药辞海.第三卷,中国医药科技出版社,1997:387
[9]邱德文,吴家荣,等.本草纲目彩色药图.草部,第13卷,贵州科技出版社,1988:155
[10]中国植物志 中国科学院植物研究所 77
[11]王天松.植物学报,1987,29(2):184
[12]黄卫东.分析化学,1998,26(9):1081
[13]《中华本草》编委会.中华本草.第三卷.上海:上海科学出版社,1999:449
[14]De Mayo P,et al.Tetrahedron,1965,21:619
[15]Kawabata Jun,et al.CA.1984.101:3909
[16]T.Takemoto,M.Uchida,K.Koike,Y.Hoshina,G.KusaNo,Chem.Pharm.Bull.1975,23,1161.
[17]高诚伟,等.高等学校化学学报,1987,8(2):141
[18]Masaaki Uchida,Yutaka Koike,Genjiro KusaNo,etal..Chem Pharm.Bull,1980,28(1),92-102
[19]Jun Kawabata,Junya Mizutani.ShizukaNolides D,E and F,Agric Biol Chem.1989,53(1),203-207
[20]徐英含,陈惜秋.及己、川乌与莽草中毒.中华内科杂志,人民卫生出版社出版,1958:6(2):74
[21]谢培山.中药制剂色谱指纹图谱(图像)鉴别.中成药,2000,22(6):391-395
[22]李克,王曙东.中草药,2003,34(11):附8-附10
[23]王夏炎.中草药,2004,35(7):附6-附9
[24]Xie P S,Yah Y Z.HPTLC fingerprint identification of ginseng[J].J High Resol Chromatogr Chromatogr Comm,1987,10(11):607-610
[25]祝晨,林朝展.黄柏药材薄层色谱特征鉴别研究.广东药学,2004,14(1):8-9
[26]洪筱坤,王志华.柴胡属19种植物挥发油的GC相对保留值的指纹图谱分析.药学学报,1988,23(1):839-845
[27]王智华,洪筱坤,包雪声.进口檀香木的GC比较分析.中国中药杂志,1991,16(1):40-43
[28]钱浩泉,李彩君,谢培山.高良姜及其近缘植物挥发油成分的气相色谱指纹图谱研究.中药新药与临床药理,2001,12(3):179-182
[29]丁平,曾元儿,何智健.不同产地阳春砂挥发油气相色谱指纹图谱研究.中国药学杂志,2004,39(6):418-420
[30]洪筱坤,王志华,李旭.HPLC-相对保留值指纹谱法鉴别大黄.中国中药杂志,1993,18(11):650-652
[31]张聪,王志华,金德庄.中国红参与高丽红参的指纹谱(HPLC-FPS)比较研究.中成药,2001,23(3):160-163
[32]曾志,杨东晖,宋力飞等.高效液相色谱指纹图谱应用于板监根的鉴定.分析化学,2002,30(7):849-852
[33]王雁,毕开顺.三七HPLC指纹图谱的建立.中国中药杂志,2003,28(4):316-320
[34]刘艳娥,吴玫涵,吴玉田等.金银花药材高效液相指纹图谱研究.解放军药学学报,2003,19(3):161-164
[35]陈士林,陈四保,徐宏喜等.葛根高效液相指纹图谱的研究.中草药,2003,34(7):661-663
[36]曾志,杨东晖,陶敬奇等.高效液相笆谱指纹图谱在中药芦根上的应用.分析化学,2004,32(8):1035-1038
[37]金永新,要林青,封士兰等.乌拉尔甘草高效液相指纹图谱的研究.中国现代应用药学,2004,21(2):93-98
[38]严启新.鸡血藤高效液相色谱指纹图谱研究.中草药,2004,35(5):556-559
[39]曹进,徐燕,张永知等.栀子药材的指纹图谱整体性分析.分析化学,2004,32(7):875-878
[40]Hasler A,Sticher O.Identification and determination of the flavoNoids from Ginkgo biloba by high-performance liquid chromatography[J].J Chromatogr,1992,605:41-48
[41]张皓冰,陶奕,洪筱坤等.HPLC-MS在麝香酮分析中的应用.中草药,2002,33(11):970-973
[42]夏泉,李绍平,黄赵刚等.三七总皂苷注射液HPLC指纹图谱的比较分析.中成药2004,26(5):345-348
[43]王钢力,戴忠,鲁静等.11种卷柏属药用植物HPCE指纹图谱的研究.中成药,2002,24(7):489-491
[44]曹敏,季一兵,陈玉英.栀子药材毛细管电泳指纹图谱的研究。中国药科大学学报,2002,33(4):284-286
[45]孙沂,隋因,郭涛等.不同产地红花药材的高效毛细管电泳指纹图谱研究.中国药学杂志,2003,38(3):176-178
[46]魏英勤,袁久荣,闫滨.黄连的毛细管电泳特征指纹图谱研究.中草药,2003,34(6):563-565
[47]宋贤丽,郭宝林,潘映红等.6种决明属药用植物种子毛细管电泳法鉴别.中国中药杂志,2003,28(6):491-496
[48]季一兵,郑朝华,陈玉英.丹参的毛细管电泳指纹图谱研究.中成药,2003,25(12):953-955
[49]孙国徉,万月生,孙毓庆.射干的毛细管电泳指纹图谱研究.色谱,2004,22(3):206-209
[50]周红涛,胡世林,冯学峰等.不同产地赤芍的FTIR指纹图谱对比分析.中草药,2002,33(9):834-837
[51]程存旧,孙跃宗.延胡索及其伪品的FTIR直接鉴别.中草药,2002,33(12):1125-1128
[52]陈畅,陈望化,李彦等.蒙山九州虫草与冬虫夏草红外光谱的比较.山东中医杂志,2004,23(2):109-110
[53]袁旭江,林励,李向明.毛橘红与光橘红的紫外光谱和薄层色谱指纹图谱研究.广州中医药大学学报,2003,20(4):305-307
[54]秦海林,赵天增.核磁共振氢谱鉴别植物中药的研究.药学学报,1999,34(1):58-62
[55]秦海林,张建新,王峥涛等.环草石斛的~H-NMR指纹图谱解析.中国中药杂志,2002,27(12):919-923
[56]秦海林,赵天增,袁卫梅等.虎杖、何首乌和掌叶大黄、唐古特大黄的~1H-NMR 指图谱解析.药学学报,1999,34(11):288-834
[57]秦海林,赵天增,尚玉俊等.苦皮藤根皮的~1H-NMR指纹图谱解析.药学学报,2001,36(6):462-466
[58]仇熙,贾晓斌,陈廉等.复方人参注射液核磁共振指纹图谱研究.中成药,2004,26(3):173-174
[59]吕扬,郑启泰,吴楠.中药材X-射线衍射图谱研究.药学学报,1997,32(3):193-198
[60]吕扬,郑启泰,章菽.中药材山奈的X衍射Fourier图分析.药学学报,1998,33(8):587-590
[61]吴云山,王树春,李兰燕等.中药材白芍的X射线衍射Fourier谱鉴定.中草药,2002,33(10):939-941
[62]刘小平,李惠,徐海星等.白花蛇舌草的X射线衍射Fourier指纹图谱鉴定.中药材,2003,26(7):488-490
[63]李晓波,伏见裕利.基于PCR技术的中药DNA分子标记鉴别.中草药,2002,33(8):760-762
[64]肖小何,刘峰群,袁海龙等.中药DNA分子标识鉴定研究进展.中草药,2000,31(8):561-564
[65]梁之桃,秦民建,王峥涛等.柴胡属5种植物RAPD分析与分类鉴定.中草药,2002,33(12):1117-1119
[66]杨美华,张大明,刘健全等.正品和伪品大黄的RAPD指纹图谱鉴定研究.中草药,2003,34(6):557-560
[67]刘文生,朱建明,何斌等.中药材厚朴的随机扩增多态性DNA指纹图谱研究.中药材,2004,27(3):164-169
[68]王夏炎.现代分析技术在中药指纹图谱研究中的应用.中草药,2004,35(7):附6-附9
[69]罗国安,王义明,曹进.多维多息特征谱及其应用.中成药,2000,22(6):395-397
[70]WHO.Guidelines for the Assesment of herbal Medicines.1996
[71]FAD Guidance for Industry-Botanical Drug Products(Draft Guidance).Ⅷ,B.2e;3e;August,2000
[72]谢培山.中药制剂色谱指纹图谱(图像)鉴别.中成药,2000,22(6):391
[73]Rudolf Bauer.Quality Criteria and Phytopharmaceutical:Can Accept Drug Standards be achieved[J].Drug information Jornal,1998,32:101
[74]EMEA.Note for Guidance on Quality of Herbal Medicinal Products.1998
[75]Young-Won and Jinwoong Kim.Tetrahedon letters 2004,45,339-341
[76]Murakami,Toshiyuki;KohNo,Kentarou;NiNomiya,Kiyofumi,etal.Chem Pharm.Bull 2001,49(8),1003-1008.
[77]Haruhisa Kizu,Shingo Kitayama,Fukio Nakatani et al.Chem Pharm Bull.1985,33(8):3324-3329.
[78]E.A.Sobolev,V.I.Grishkovets,A.S.Shashkov et al.Chemistry of Natural compounds.2000,36(5):538-539.
[79]赵萍萍.药学学报,1987,22(1):70-74
[80]李创军,张东明,罗永明 宽叶金粟兰化学成分的研究2005 40(6):525-528.
[81]赵萍萍.药学学报,1987,22(1):70-74
[82]王玺,王文宁,张克荣等.中药HPLC指纹图谱相似性研究的探讨.沈阳药科大学学报,2003,20(5):360-362
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