转录因子stat3调控激活态雪旺细胞基因表达的研究
|
摘要
引言：对周围神经损伤伴缺损的治疗仍是外科领域的一个难题，目前主要是通过自体神经移植进行缺损段的修复，这种治疗方法本身存在一些不足。组织工程化人工神经为周围神经缺损修复提供了新的手段，随着组织工程材料研究的不断发展和对于组织工程神经认识的不断深入，使得组织工程化人工神经的发展成为一个非常有前景的领域。而激活态雪旺细胞(activated Schwann cell ASC)作为一种优良的组织工程种子细胞，具有高增值，高分泌某些神经营养因子活性，能够有效促进周围神经再生。对该细胞生物学活性的系统研究，是人工神经研究的另一个热点。本课题从转录因子、基因水平上对激活态雪旺细胞进行研究，寻找激活态雪旺细胞优于正常态雪旺细胞(normal Schwann cell NSC)的生物活性的原因。共分为三个部分：
第一部分使用黑素细胞培养基改良成年大鼠雪旺细胞培养方法
目的寻找简单、快速获得高纯度雪旺细胞的方法。方法20只预变性一周的成年SD大鼠，重150克，取其坐骨神经，经0.25％胰酶，0.1％胶原酶分次酶消化30分钟后。分别使用DMEM／F12培养基(A组)和黑素细胞培养基(B组)进行培养。冷喷射(coldjet)方式传代。S-100染色鉴定并计算雪旺细胞纯度。结果：采用黑素细胞培养基组的雪旺细胞纯度达到90％以上，细胞收获量远高于采用DMEM／F12培养基的对照组。而对照组的雪旺细胞纯度仅能保持在30％左右。
结论：使用黑素细胞培养基及冷喷射传代操作简单，可以在短期内获得足量高纯度的雪旺细胞。
第二部分应用转录因子蛋白／DNA组合芯片检测激活态／正常态雪旺细胞的活性转录因子表达水平的差异
目的检测激活态／正常态雪旺细胞活性转录因子及其表达水平上的差异。方法应用转录因子蛋白／DNA组合芯片技术检测激活态／正常态雪旺细胞的活性转录因子。结果激活态雪旺细胞和正常态雪旺细胞的转录因子芯片点阵图显示：激活态雪旺细胞与正常态雪旺细胞相比，有6个转录因子蛋白上调5倍以上，它们是PEPCK promoter GR、NF-E2(2)、Stat3、NFE-6／CP1、PRDII-BF1和NFkB(2)；这些表达显著增加的转录因子，可能就是激活态雪旺细胞增殖活性高重要的原因；结论6个转录因子蛋白上调都与激活态雪旺细胞的快速分裂、高分泌状态密切相关。
第三部分染色质免疫沉淀结合芯片技术检测转录因子stat3调控激活态雪旺细胞／正常态雪旺细胞基因表达
目的检测转录因子stat3调控激活态雪旺细胞／正常态雪旺细胞哪些基因的表达，发现ASC／NSC在基因表达水平的差异方法采用染色质免疫沉淀结合芯片技术对ASC／NSC基因组进行高通量筛选结果经过对ASC／NSC全基因组385，000个位点进行扫描后，发现有361种基因在ASC／NSC中都与转录因子stat3发生结合。另有122种基因，仅在ASC中与stat3结合，而NSC中这些基因并不与stat3发生作用。其中的一些基因如：Atoh1、bex1、F2RL1、polg、Rcan2主要参与细胞周期、凋亡和成髓的调控。结论仅在ASC中与转录因子stat3发生作用的基因，与ASC促进神经轴突再生，抑制凋亡，成髓，维持线粒体功能稳定等功能密切相关。
Introduction
It remains as a clinical challenge in the treatment of peripheral nervedefects. The current gold standard for the bridging of significantperipheral nerve defects is autologous nerve grafting. This method hasbeen shown to be effective, but itself has some disadvantages which havelimited value in clinical practice. This has shifted the focus of thesearch to tissue engineering conduits. Following by the development ofconduits materials research and the cognition of mechanism of peripheralnerve pathology, tissue engineering has turned into a promising protocolin bridging peripheral nerve defects. Activated Schwann cell (ASC), asthe ideal seed cell of tissue engineering conduits, has the ability ofquick proliferation and to actively stimulate repair with neurotrophicfactors An active area of research is the systemic research of themechanism of its high bio-activity. The objective of this study is to findout the reason why the ASC could promote axonal regeneration across longerdefects prior to the normal Schwann cell (NSC) at the transcript and genelevel. Three parts involved in this research, following as:
Part 1 Application of melanocyte growth medium in the cell culture ofSchwann cell from adult SD rats
Objective: To find out a effective approach to obtain high purifiedSchwann cells from the sciatic nerve of adult rats
methods: 20 SD rats with 1 week predegeneration , weight: 150g, afterenzymatic digestion(0.25% Trypsin, 0.1% collagenase) of sciatic nerve,the suspension were cultured in group A (DMEM/F12 medium) and group B(melanocyte growth medium).cold jet as the passage method. S-100 stainfor detection of Schwann cell purity.
Results: the cell yields and purity of group B was significant higher thanthat of group A. as the contrast, the control group, which has only 30%purity of Schwann cell.
conclusions: the combination of melanocyte growth medium and cold jetprovides a convenient mean to obtain considerable Schwann cell with purity beyond 90%.
Part 2 To identify the activated transcription factors of activatedSchwann cell using TranSignal~(TM) Protein/DNA Array techniques
Objective To identify the activated transcription factors ofactivated Schwann cell and normal Schwann cell. Methods TranSignal~(TM)Protein/DNA Array techniques were adopted to scan the activatedtranscription factors. Results Lattice of activated Schwann cellsshowed that there were 6 different transcription factors raised theirexpression 5 times than that of normal Schwann cells. Including PEPCKpromoter GR、NF-E2(2)、Stat3、NFE-6/CP1、PRDⅡ-BF1、NFkB(2).which may giveus the possible explanation of special condition of activated Schwanncells at transcript level. Conclusion Total 6 different transcriptionfactors correlated their level with activated Schwann cells' quickproliferation and vigorous secretion condition.
Part3 Identifies the primary target genes of transcript factor stat3in activated Schwann cell/normal Schwann cell by the approach ofChromatin immunoprecipitation on chip assay(ChIP on chip)
Objective to identify the primary target genes that directly under controlof transcript factor star3 in activated Schwann cell and normal Schwanncell, and to find out the possible differences between ASC and NSC tounderstand the mechanism that why ASC perform more bioactivity at the genelevel. Method scanning the whole genome of ASC/NSC by the application ofChromatin immunoprecipitation on chip assay(ChIP on chip),immunoprecipitated the target gene with specific antibody of transcriptfactor star3 Result 385,000 genes were scanned in ASC/NSC, 361 of whichwere bound to the stat3 both in ASC and NSC, we identified 122 kind of genes that only bind to star3 in ASC, some of these genes are "Atoh1、bex1、F2RL1、polg、Rcan2" responsible for the quick proliferation ofASC and has the protective effect from apoptosis. Conclusion the differenttarget gene of star3 in ASC/NSC may explain the mechanism of ASC highbioactivity.
第一部分使用黑素细胞培养基改良成年大鼠雪旺细胞培养方法
目的寻找简单、快速获得高纯度雪旺细胞的方法。方法20只预变性一周的成年SD大鼠，重150克，取其坐骨神经，经0.25％胰酶，0.1％胶原酶分次酶消化30分钟后。分别使用DMEM／F12培养基(A组)和黑素细胞培养基(B组)进行培养。冷喷射(coldjet)方式传代。S-100染色鉴定并计算雪旺细胞纯度。结果：采用黑素细胞培养基组的雪旺细胞纯度达到90％以上，细胞收获量远高于采用DMEM／F12培养基的对照组。而对照组的雪旺细胞纯度仅能保持在30％左右。
结论：使用黑素细胞培养基及冷喷射传代操作简单，可以在短期内获得足量高纯度的雪旺细胞。
第二部分应用转录因子蛋白／DNA组合芯片检测激活态／正常态雪旺细胞的活性转录因子表达水平的差异
目的检测激活态／正常态雪旺细胞活性转录因子及其表达水平上的差异。方法应用转录因子蛋白／DNA组合芯片技术检测激活态／正常态雪旺细胞的活性转录因子。结果激活态雪旺细胞和正常态雪旺细胞的转录因子芯片点阵图显示：激活态雪旺细胞与正常态雪旺细胞相比，有6个转录因子蛋白上调5倍以上，它们是PEPCK promoter GR、NF-E2(2)、Stat3、NFE-6／CP1、PRDII-BF1和NFkB(2)；这些表达显著增加的转录因子，可能就是激活态雪旺细胞增殖活性高重要的原因；结论6个转录因子蛋白上调都与激活态雪旺细胞的快速分裂、高分泌状态密切相关。
第三部分染色质免疫沉淀结合芯片技术检测转录因子stat3调控激活态雪旺细胞／正常态雪旺细胞基因表达
目的检测转录因子stat3调控激活态雪旺细胞／正常态雪旺细胞哪些基因的表达，发现ASC／NSC在基因表达水平的差异方法采用染色质免疫沉淀结合芯片技术对ASC／NSC基因组进行高通量筛选结果经过对ASC／NSC全基因组385，000个位点进行扫描后，发现有361种基因在ASC／NSC中都与转录因子stat3发生结合。另有122种基因，仅在ASC中与stat3结合，而NSC中这些基因并不与stat3发生作用。其中的一些基因如：Atoh1、bex1、F2RL1、polg、Rcan2主要参与细胞周期、凋亡和成髓的调控。结论仅在ASC中与转录因子stat3发生作用的基因，与ASC促进神经轴突再生，抑制凋亡，成髓，维持线粒体功能稳定等功能密切相关。
Introduction
It remains as a clinical challenge in the treatment of peripheral nervedefects. The current gold standard for the bridging of significantperipheral nerve defects is autologous nerve grafting. This method hasbeen shown to be effective, but itself has some disadvantages which havelimited value in clinical practice. This has shifted the focus of thesearch to tissue engineering conduits. Following by the development ofconduits materials research and the cognition of mechanism of peripheralnerve pathology, tissue engineering has turned into a promising protocolin bridging peripheral nerve defects. Activated Schwann cell (ASC), asthe ideal seed cell of tissue engineering conduits, has the ability ofquick proliferation and to actively stimulate repair with neurotrophicfactors An active area of research is the systemic research of themechanism of its high bio-activity. The objective of this study is to findout the reason why the ASC could promote axonal regeneration across longerdefects prior to the normal Schwann cell (NSC) at the transcript and genelevel. Three parts involved in this research, following as:
Part 1 Application of melanocyte growth medium in the cell culture ofSchwann cell from adult SD rats
Objective: To find out a effective approach to obtain high purifiedSchwann cells from the sciatic nerve of adult rats
methods: 20 SD rats with 1 week predegeneration , weight: 150g, afterenzymatic digestion(0.25% Trypsin, 0.1% collagenase) of sciatic nerve,the suspension were cultured in group A (DMEM/F12 medium) and group B(melanocyte growth medium).cold jet as the passage method. S-100 stainfor detection of Schwann cell purity.
Results: the cell yields and purity of group B was significant higher thanthat of group A. as the contrast, the control group, which has only 30%purity of Schwann cell.
conclusions: the combination of melanocyte growth medium and cold jetprovides a convenient mean to obtain considerable Schwann cell with purity beyond 90%.
Part 2 To identify the activated transcription factors of activatedSchwann cell using TranSignal~(TM) Protein/DNA Array techniques
Objective To identify the activated transcription factors ofactivated Schwann cell and normal Schwann cell. Methods TranSignal~(TM)Protein/DNA Array techniques were adopted to scan the activatedtranscription factors. Results Lattice of activated Schwann cellsshowed that there were 6 different transcription factors raised theirexpression 5 times than that of normal Schwann cells. Including PEPCKpromoter GR、NF-E2(2)、Stat3、NFE-6/CP1、PRDⅡ-BF1、NFkB(2).which may giveus the possible explanation of special condition of activated Schwanncells at transcript level. Conclusion Total 6 different transcriptionfactors correlated their level with activated Schwann cells' quickproliferation and vigorous secretion condition.
Part3 Identifies the primary target genes of transcript factor stat3in activated Schwann cell/normal Schwann cell by the approach ofChromatin immunoprecipitation on chip assay(ChIP on chip)
Objective to identify the primary target genes that directly under controlof transcript factor star3 in activated Schwann cell and normal Schwanncell, and to find out the possible differences between ASC and NSC tounderstand the mechanism that why ASC perform more bioactivity at the genelevel. Method scanning the whole genome of ASC/NSC by the application ofChromatin immunoprecipitation on chip assay(ChIP on chip),immunoprecipitated the target gene with specific antibody of transcriptfactor star3 Result 385,000 genes were scanned in ASC/NSC, 361 of whichwere bound to the stat3 both in ASC and NSC, we identified 122 kind of genes that only bind to star3 in ASC, some of these genes are "Atoh1、bex1、F2RL1、polg、Rcan2" responsible for the quick proliferation ofASC and has the protective effect from apoptosis. Conclusion the differenttarget gene of star3 in ASC/NSC may explain the mechanism of ASC highbioactivity.
引文
[1] RaviV. B. Peripheral nerve regeneration: an opinion on channels, scaffolds and anisotropy [J]. Biomaterials. 2006, 27:3515-3518
[2] Nichols CM, Brenner MJ, Fox IK, et al. Effects of motor versus sensory nerve grafts on peripheral nerve regeneration [J]. Exp Neurol 2004; 190(2): 347-55.
[3] 劳杰，熊良俭，顾玉东，等．应用激活态的雪旺细胞填充导管修复周围神经缺损的初步研究[J]．中华手外科杂志．2000：16(4)：236-240
[4] Gregory. RD. Peripheral nerve injury: a review and approach to tissue engineered constructs [J]. Anat Rec. 2001, 263:396-404
[5] Choi BH, Zhu SJ, Kim SH, et al. Nerve repair using a vein graft filled with collagen gel [J]. J Reconstr Microsurg, 2005,21(4):267-72
[6] Mohammad. J, Shenaq. J, Rabinovsky. E, et al. Modulation of peripheral nerve regeneration: a tissue-engineering approach. The role of amnion tube nerve conduit across a 12 centimeter nerve gap [J]. Plast Reconstr Surg, 2000, 105 (2): 660-666.
[7] Geuna. S, Tos. P, Battiston. B, et al. Bridging peripheral nerve defects with muscle- vein combined guides [J]. Neurol Res, 2004, 26(2):139-44.
[8] Chenite. A, Chaput. C, Wang. D, et al. Novel injectable neutral solutions of chitosan form biodegradable gels in situ [J]. Biomaterials, 2000, 21: 2155-2161.
[9] Yoshii S, Shima M, Oka M, et al. Nerve regeneration along collagen filament and the presence of distal nerve stump [J]. Neurol Res, 2004, 26(2):145-50.
[10] Varon. S, Williams. IR. Peripheral nerve regeneration in a silicone model chamber: cellular and molecular aspects[J]. Peripheral Nerve Repair and Regeneration, 1986, 1: 9-15
[11] James. D, Arundathi. R, Les. O, et al. The Ability of Human Schwann Cell Grafts to Promote Regeneration in the Transected Nude Rat Spinal Cord[J]. Exp Neurol, 1997, 148: 502-522
[12] Fox. IK, Schwetye. KE, Keune. JD. Schwann cell injection of cold-preserved nerve allografts [J]. Microsurgery, 2005, 25(6):502-7.
[13] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells []]. J Neurosci Res, 2004, 77: 453-461.
[14] Paul. JK, Daniel. FK, Daljeet. M, et al. Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro [J]. Exp Neurol, 2007, 207:267-274
[15] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000：16(3)：172-174
[16] 劳杰，熊良俭，赵新，等．不同时段预变神经雪旺细胞的形态学变化[J]．中华手外科杂志．2001：21(11)：689-691
[17] 蒋良福，劳杰，何继银等．几丁糖胶原复合膜及激活态雪旺细胞修复周围神经缺损的实验研究[J]．中华手外科杂志，2005，21(3)：172-174
[18] 劳杰，蒋良福，顾玉东．激活态雪旺细胞生长相关蛋白43mRNA表达的研究[J]．中华骨科杂志，2005，25(6)：372-374
[19] 劳杰，蒋良福，顾玉东等，脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003 19(2)：109-111
[20] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002 18(2)：174-176
[21] 何继银，劳杰，蒋良福等，激活态雪旺细胞信号转导通路的初步研究[J]．中华手外科杂志，2006，22(6)：376-378
[1] 劳杰，熊良俭，顾玉东，等．改良成年SD大鼠雪旺细胞培养的实验研究．中华手外科杂志[J]，1999，15：106-108．
[2] Michael E. Pannunzio, I-ming J. A new method of selecting Schwann cells from adult mouse sciatic nerve [J]. J Neurosci Methods 2005, 149: 74-81
[3] Jirsova. K, Sodaar. P, Mandys. V. et al. Cold jet: a method to obtain pure Schwann cell cultures without the need for cytotoxic, apoptosis inducing drug treatment. J Neurosci Methods [J]1997,78:133-137
[4] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells [J]. J Neurosci Res, 2004, 77: 453-461.
[5] Gerburg. K, Hisham. F, Wolfgang. S et al. In vivo predegeneration of peripheral nerves an effective technique to obtain activated Schwann ceils for nerve conduits [J]. J Neurosci Methods, 1999,89:17-24
[6] Diany CM, Zayra G, Clara S, et al. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C) [J]. J neurosci Methods, 2002, 114: 1-8
[7] Yu QJ, Wei L, Tan HH, et al. Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment [J]. J neurosci Methods, 2008, 170:140-148.
[8] Heldin NE, Paulsson Y, Forsberg K, et al. Inductionof cyclic AMP synthesis by forskolin is followed by a reduction in the expression of c-myc messenger RNA and inhibition of 3H-thymidine incorporation in human fibroblasts [J]. J Cell Physiol 1989, 138:17-23.
[9] Chang JY, Brown S. Cytosine arabinoside differentially alters survival and neurite outgrowth of neuronal PC12 cells [J]. Biochem Biophys Res Commun 1996, 218: 753-8.
[10] Fields K, Raine C. Ultrastructure and immunocytochemistry of rat Schwann cells and fibroblasts in vitro [J]. J Neuroimmunol. 1982, 2: 155-166.
[11] Maurice V, Norbert W. Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve [J]. J Neurosci Methods, 2003,124:135-143
[12] Komiyama T, Nakao Y, Toyama Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit [J]. J Neurosci Methods, 2003, 122: 195-200.
[13] 胡晞棠，熊良俭，劳杰．从成年大白鼠坐骨神经分离培养许旺细胞的方法学探讨[J]．中华显微外科杂志，2002，25：197-200．
[14] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[15] Minghetti. L, Goodearl. ADJ, Mistry. K, et al. Glial growth factors Ⅰ-Ⅲ are specific mitogens for glial cells [J]. J Neurosci Res, 1996, 43:684-693.
[16]劳杰，姜良福，顾玉东，等．脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003，19：109-110．
[17]Haynes LW, Rushton JA, Perrins MF, et al. Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formation in vivo [J]. J Neurosci Methods 1994, 52:119-27.
[1] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[2] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000：16(3)：172-174
[3] 劳杰，熊良俭，赵新，等．不同时段预变神经雪旺细胞的形态学变化[J]．中华手外科杂志．2001：21(11)：689-691
[4] 劳杰，熊良俭，顾玉东，等．应用激活态的雪旺细胞填充导管修复周围神经缺损的初步研究[J]．中华手外科杂志．2000：16(4)：236-240
[5] 蒋良福，劳杰，何继银等．几丁糖胶原复合膜及激活态雪旺细胞修复周围神经缺损的实验研究[J]．中华手外科杂志，2005，21(3)：172-174
[6]劳杰，姜良福，顾玉东，等．脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003，19：109-110．
[7]Stafford. JM, Wilkinson. JC, Beechem. JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter[J]. J Biol Chem. 2001, 276(43): 39885-39891.
[8]Raltreider. RC, Davis. AM, lariviere. JP. et al. Arsenic alters the function of the glucocorticoid receptor as a transcription factor[J]. Environ Health Perspect. 2001, 109(3):245-251.
[9]Scott. DK, Stromsted. PE, Wang. JC, et al. Further characterization of the glucocorticoid response unit in the phosphoenolpyruvate carboxykinase gene. The role of the glucocorticoid receptor-binding sites[J]. Mol. Endo. 1998, 12(4):482-491.
[10]Imai. E, Miner. JN, Mitchell. JA, et al. Glucocorticoid receptor-cAMP response element-binding protein interaction and the response of the phosphoenolpyruvate carboxykinase gene to glucocorticoids[J]. J Biol Chem. 1993, 268(8): 5353-5356.
[11]Marhenke. S, Lamle. J, Buitrago-Molina. LE, et al. A novel role of the NRF2 transcription factor in the regulation of arsenite-mediated keratin 16 gene expression in human keratinocytes[J]. Hepatology. 2008,48(2): 487-496.
[12]Williams. MA, Rangasamy. r, Bauer. SM, et al. Disruption of the transcription factor Nrf2 promotes pro-oxidative dendritic cells that stimulate Th2-like immunoresponsiveness upon activation by ambient particulate matter[J]. J Immuno. 2008,181:4545-4559.
[13]Alam. J, Cook. JL. How many transcription factors does it take to turn on the heme oxygenase-1 gene[J]. Am J Respir Cell Mol Biol. 2007, 36:166-174.
[14]Huang. A, Zhang. YY, Chen.K. et al. Cytokine-stimulated GTP cyclohydrolase I expression in endothelial cells requires coordinated activation of nuclear factor-kappaB and Statl Stat3[J]. Circ Res.2005,96:164-171.
[15]Legeendre. F, Dudhia. J, Pujol.JP. et al. JAK STAT but not ERK1 ERK2 pathway mediates interleukin (IL) -6 soluble IL-6R down-regulation of Type Ⅱ collagen[J]. J Biol Chem. 2003, 278(5):2903-2912.
[16]Schumann. G, Huell.M, Machein.U. et al. Interleukin-6 activates signal transducer and activator of transcription and mitogen-activated protein kinase signal transduction pathways and induces de novo protein synthesis in human neuronal cells [J]. J Neurochem. 1999,73:2009-2017.
[17]Niehof.M, Streetz. K, Rakemann. T, et al. Interleukin-6-induced tethering of STAT3 to the LAP C EBPbeta promoter suggests a new mechanism of transcriptional regulation by STAT3 [J]. J Biol Chem. 2001, 276(12):9016-9027.
[18]Colin. Y, Joulin.V, Kim. CLV. et al. Characterization of a new erythroid megakaryocyte-specific nuclear factor that binds the promoter of the housekeeping human glycophorin C gene[J]. J Biol Chem. 1990,265(28):16729-16732.
[19]Profita.M, Bonanno. A, Siena.L. et al. Acetylcholine mediates the release of IL—8 in human bronchial epithelial cells by a NFkB ERK-dependent mechanism[J]. Eur J Pharmacol. 2008,582:145-153.
[20]Novotny.NM, Markel.TA, Crisostomo.PR, et al. Differential IL-6 and VEGF secretion in adult and neonatal mesenchymal stem cells role of NFkB[J]. Cytokine. 2008,43:215-219.
[2l]Grieder.NC, Nellen.D, Burke. R, et al. Schnurri is required for Drosophila Dpp signaling and encodes a zinc finger protein similar to the mammalian transcription factor PRDII-BF1[J]. Cell. 1995, 81:791-800.
[22]Muchardt.C, Seeler. JS, Nirula. A, et al. Regulation of human immunodeficiency virus enhancer function by PRDII-BF1 and c-rel gene products[J]. J Virol. 1992, 66(1): 244-250.
[23]Lin. R, Gewert. D, Hiscott.J. Differential transcriptional activation in vitro by NF-kappa B Rel proteins[J]. J Biol Chem. 1995,270(7): 3123-3131.
[1]Wang. JC, Derynck. MK, Nonaka. DF et al. Chromatin immunoprecipitation (ChIP) scanning identifies primary Glucocorticoid receptor target genes[J]. Proc. Natl. Acad. Sci. 2004, 101(44):15603-15608
[2]Kondo.Y, Shen. L, Yan. PS, et al. Chromatin immunoprecipitation microarrays for identification of genes silenced by histone H3 lysine 9 methylation[J]. Proc. Natl. Acad. Sci. 2004, 101(19):7398-7403
[3]Lavelle. D, Vaitkus.K, Hankewych. M, et al. Developmental changes in DNA methylation and covalent histone modifications of chromatin associated with the ε-, γ -, and β-globin gene promoters in Papio anubis[J]. Blood cells, Molecules, and diseases. 2006,36:269-278.
[4]Lavelle. D, Vaitkus.K, Hankewych. M, et al. Effect of 5-aza-2 '-deoxycytidine (Dacogen) on covalent histone modifications of chromatin associated with the -, -, and β-globin promoters in Papio anubis[J].Exp Hematol. 2006,34:339-347.
[5]Dann.SG, Allison.T, Veldhoen. K, et al. Chromatin immunoprecipitation assay on the rainbow trout opsin proximal promoters illustrates binding of NF- κ B and c-jun to the SWS1 promoter in the retina[J]. Exp Eye Res.2004,78: 1015-1024
[6]Weber. M, Davies. JJ, Wittig. D, et al. Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells[J]. Nat Genet.2005, 37 (8): 853-862.
[7]Buck. MJ, Lieb. JD. ChIP-chip: considerations for the design analysis and application of genome-wide chromatin immunoprecipitation experiments[J]. Genomics. 2004,83:349-360.
[8]Arie. NB, McCll.AE, Berkman. s, et al. Evolutionary conservation of sequence and expression of the bHLH protein Atonal suggests a conserved role in neurogenesis[J]. Human Mol Genet. 1996, 5(9):1207-1216.
[9]Seipel.K, Yanze.N, Schmid. V. Developmental and evolutionary aspects of the basic helix-loop-helix transcription factors Atonal-like 1 and Achaete-scute homolog 2 in the jellyfish[J]. Dev Biol. 2004, 269: 331 -345.
[10]Briggs. KJ, Schwartz. IM, Zhang. W, et al. Cooperation between the Hicl and Ptchl tumor suppressors in medulloblastoma[J]. Gen Dev. 2008,22:770-785.
[11]Lin. W, Kemper. A, McCarthy, Et al. Interferon-gamma induced medulloblastoma in the developing cerebellum [J]. JNeurosci. 2004,24(45):10074-10083.
[12]Vilar. M, Murillo-Carretero. M, Mira.H, et al. Bexl, a novel interactor of the p75 neurotrophin receptor, links neurotrophin signaling to the cell cycle[J]. EMBO. 2006, 25:1219-1230.
[13] Koo. JH, Saraswati.M, Margolis.FL. Immunolocalization of Bex protein in the mouse brain and olfactory system[J]. J Comp Neurol. 2005, 487:1-14.
[14]Koo. JH, Smiley. MA, Lovering. RM, et al. Bexl knock out mice show altered skeletal muscle regeneration[J]. Biochem Biophys Res Commun.2007,363:405-410.
[15]Alvarez.E, Zhou.W, Witta. SE, et al. Characterization of the Bex gene family in humans, mice, and rats[J]. Gene. 2005,357:18-28.
[16]Nystedt. S, Emilsson. K, Wahlestedt.C, et al. Molecular cloning of a potential proteinase activated receptor [J]. Proc Natl Acad Sci. 1994, 91:9208-9212.
[17]01ianas. MC, Dedoni.S, Onali.P. Proteinase-activated receptors 1 and 2 in rat olfactory system: layer-specific regulation of multiple signaling pathways in the main olfactory bulb and induction of neurite retraction in olfactory sensory neurons [J]. Neurosci. 2007, 146:1289-1301.
[18]Costa. R, Marotta. DM, Manjavachi.MN. et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice[J]. Br J Pharmacol. 2008,154:1094-1103.
[19]Luo. WB, Wang. YF, Reiser. G. Protease-activated receptors in the brain receptor expression, activation, and functions in neurodegeneration and neuroprotection[J]. Brain Res Rev. 2007, 56: 331-345.
[20] Wang. YF, Luo. WB, Reiser.G. Activation of protease-activated receptors in astrocytes evokes a novel neuroprotective pathway through release of chemokines of the cytokine-induced neutrophil chemoattractant family[J]. Eur J Neurosci. 2007, 25: 3159-3168.
[21]Park. GH, Ryu. JR, Shin. CY, et al. Evidence that protease-activated receptor-2 mediates trypsin-induced reversal of stellation in cultured rat astrocytes[J]. Neurosci Res. 2006, 54: 15-23.
[22]Taanman. JW, Rahman. S, Pagnamenta. AT, et al. Analysis of mutant DNA polymerase gamma in patients with mitochondrial DNA depletion[J].Hum Murat. 2009, 30: 248-254.
[23]Ueta. E, Sasabe. E, Yang. Z, et al. Enhancement of apoptotic damage of squamous cell carcinoma cells by inhibition of the mitochondrial DNA repairing system[J]. Cancer Sci. 2008, 99(11):2230-2237.
[24] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000：16(3)：172-174
[25] 劳杰，蒋良福，顾玉东．激活态雪旺细胞生长相关蛋白43mRNA表达的研究[J]．中华骨科杂志，2005，25(6)：372-374
[26] 劳杰，蒋良福，顾玉东等，脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003 19(2)：109-111
[27]Facchin. F, Canaider. S, Vitale. L, et al. Identification and analysis of human RCAN3 (DSCR1L2) mRNA and protein isoforms[J].Gene. 2008, 407: 159-168.
[28]Porta. S, Marti. E, Luna. S, et al. Differential expression of members of the RCAN family of calcineurin regulators suggests selective functions for these proteins in the brain[J]. Eur J Neurosci. 2007,26:1213-1226.
[29]Rodriguez. P. Oligodendrocyte development and thyroid hormone[J].J Neurobiol. 1999, 40: 497-512.
[1] 劳杰，熊良俭，顾玉东，等．改良成年SD大鼠雪旺细胞培养的实验研究[J]．中华手外科杂志，1999，15：106-108．
[2] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells[J]. J Neurosci Res, 2004, 77: 453-461.
[3] Diany CM, Zayra G, Clara S, et al. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C) [J]. J Neurosci Methods, 2002,114:1-8
[4] Maurice V, Norbert W. Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve [J]. J Neurosci Methods, 2003,124:135-143
[5] Yu QJ, Wei L, Tan HH, et al. Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment [J]. J Neurosci Methods, 2008, 170:140-148.
[6] Komiyama T, Nakao Y, Ioyama Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit [J]. J Neurosci Methods, 2003, 122: 195-200.
[7] 胡晞棠，熊良俭，劳杰．从成年大白鼠坐骨神经分离培养许旺细胞的方法学探讨[J]．中华显微外科杂志，2002，25：197-200．
[8] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[9] 劳杰，姜良福，顾玉东，等．脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003，19：109-110．
[1] RaviV. B . Peripheral nerve regeneration: an opinion on channels,scaffolds and anisotropy [J]. Biomaterials. 2006, 27: 3515-3518
[2] Sunderland S. Rate of regeneration of I : sensory nerve fibres and II: motor fibres [J]. Arch Neurol Psychiatry, 1947, 58:1 - 14.
[3] MacKinnon SE. New directions in peripheral nerve surgery [J]. Ann Plast Surg, 1989, 22 (3) :257 - 273.
[4] Mackinnon. SE, Dellon. AL. Anatomy and physiology of the peripheral nerve. Surgery of the peripheral nerve [M]. New York: Thieme, 1988:1 -33.
[5] Nichols CM, Brenner MJ, Fox IK, et al. Effects of motor versus sensory nerve grafts on peripheral nerve regeneration [J]. Exp Neurol 2004;190(2) :347-55.
[6] Millesi. H, Meissl. G, Berger. A. Further experience with interfascicular grafting of the median, ulnar and radial nerves [J]. J Bone Joint Surg. 1976, 58: 209-218.
[7] Rutkowski. GE, Heath. CA. Development of a Bioartificial Nerve Graft. Design Based on a Reaction-Diffusion Model [J]. Biotechnol.Prog. 2002; 18:362-372
[8] Rutkowski. GE, Heath. CA. Development of a Bioartificial Nerve Graft.Nerve Regeneration in vitro Biotechnol[J]. Prog. 2002; 18:373-379
[9] Hu M, Sabelman. EE, Tsai. C, et al. Improvement of Schwann Cell Attachment and proliferation on Modified Hyaluronic Acid Strands by Polylysine [J]. Tissue Eng, 2000; 6(6):585-593
[10] Hadlock. T, Sundback. C, Hunter. D, et al. A polymer Conduit seeds with Schwann Cells Promotes Guided Peripheral Nerve Regeneration [J].Tissue Eng, 2000; 6(2):119-127
[11] Fox.IK, Schwetye. KE, Keune. JD. Schwann cell injection of cold-preserved nerve allografts [J]. Microsurgery, 2005, 25(6):502-7.
[12] Mosahebi. A, Fuller. P, Wiberg. M, et al. Effect of allogeneic Schwann cell transplantation on peripheral nerve regeneration [J]. Exp Neurol,2002,173(2): 213-23.
[13]Fansa. H, Dodic. T, Wolf. G, et al. Tissue engineering of peripheral nerves Epineurial grafts with application of cultured Schwann cells [J].Microsurgery, 2003, 23: 72-77
[14] Schlosshauer. B, Muller. E, Schroder. B et al. Rat Schwann cells in bioresorbable nerve guides to promote and accelerate axonal regeneration [J]. Brain Res, 2003, 963: 321-326.
[15] Frerichs. 0, Fansa. H, Schicht.C. et al. Reconstruction of peripheral nerves using acellular nerve grafts with implanted cultured Schwann cells[J]. Microsurgery, 2002, 22: 311-315
[16] Rodriquez.FT, Verdu. E, Ceballos. D. Nerve guides seeded with autologous Schwann cells improve nerve regeneration[J ]. Exp Neurol.2000; 161:571—84.
[17] Lundborg G, Dahlin LB. Reorganisation and orientation of regeneration nerve fibers , perineurium , and epineurium in preformed mesothelial bubes-an experimental study on the sciatic nerve of rats[J ] . J Neurosci Res, 1981, 6 :256 - 281.
[18] Choi BH, Zhu SJ, Kim SH, et al. Nerve repair using a vein graft filled with collagen gel [J]. J Reconstr Microsurg, 2005, 21(4):267-72
[19] Mohammad. J, Shenaq. J, Rabinovsky. E, et al. Modulation of peripheral nerve regeneration: a tissue-engineering approach. The role of amnion tube nerve conduit across a 12 centimeter nerve gap [J]. Plast Reconstr Surg, 2000, 105 (2): 660-666.
[20]Geuna. S, Tos. P, Battiston. B, et al. Bridging peripheral nerve defects with muscle- vein combined guides [J]. Neurol Res. 2004,26(2): 139-44.
[21]Keilhoff. G, Pratsch. F, Wolf. G, et al. Bridging extra large defects of peripheral nerves: possibilities and limitations of alternative biological grafts from acellular muscle and Schwann cells [J]. Tissue Eng,2005; 11(7):1004-1014
[22] Norris R W, Glasby M A. Peripheral nerx re repair in numaus using muscle autografts[J]. J Bone and Joint Surg, 1988,70:530-537
[23]Mohammad. J, Shenaq. J, Rabinovsky. E,. et al . Modulation of peripheral nerve regeneration: a tissue-engineering approach. The role of amnion tube nerve conduit across a 12centimeter nerve gap [J]. Plast Reconstr Surg, 2000, 105 (2): 660-666.
[24]Liu. W, Cui.L, Cao.YL. A closer view of tissue engineering in China: the experience of tissue construction in immunocompetent animals[J]. Tissue Eng, 2003; 9(s1):17-31
[25]Yuan. Y, Zhang. P, Yang. YM, et al. The interaction of Schwann cells with chitosan membranes and fibers in vitro[J]. Biomaterials. 2004, 25: 4273-4278
[26]Wei. X, Lao. J, Gu. YD. Bridging peripheral nerve defect with chitosan-collagen film[J].Chin J Traumatol. 2003, 6(3):131-4.
[27]Varon S, William s IR. Peripheral nerve regeneration in a silicone model chamber:cellular and molecular aspects[J]. Peripheral Nerve Repair and Regeneration, 1986, 1:9-15
[28]Ceonzo K, Gaynor A, Shaffer L, Polyglycolic Acid-Induced Inflammation: Role of Hydrolysis and Resulting Complement Activation [J]. Tissue Eng, 2006, 12(2):301-308.
[29]Richard. B. Cellular engineering: molecular repair of membranes to rescue cells of the damaged nervous system [J]. Neurosurgery. 2001, 49(2):370-379
[30]Wells. MR, Kraus. K, Batter. DK, et al. Gel matrix vehicles for growth factor application in nerve gap injurys repaired with tube: a comparison of biomatrix, collagen, and methylcellulose. Exp Neurol. 1997, 146: 395-402
[31] 何继银，劳杰．周围神经损伤后雪旺细胞生存与凋亡的动态平衡[J]．中华创伤骨科杂志，2004 6(3)：330-333
[32]Popovic. M, Sketelj. J, Bresjanac. M. Changes of Schwann cell antigenic profile after peripheral nerve injury[J]. Eur J Physiol. 1996,431:287-288
[33]Singh. N, Birdi. TJ, Chandrashekar. S, et al. Schwann cell extracellular matrix protein production is modulated by Mycobacterium leprae and macrophage secretory products [J]. J Neurol SCi, 1997, 151:13-22.
[34] Tada H, Hatoko M, Tanaka A, et al. Detection of E-cadherin expression after nerve repair in a rat Sciatic nerve model[J]. Ann Plast Surg, 2001, 47: 178-182.
[35] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells [J]. J Neurosci Res, 2004, 77: 453-461.
[36]Manent. J, Oguievetskaia. K, Bayer. J, et al. Magnetic cell sorting for enriching Schwann cells from adult mouse peripheral nerves [J]. J Neurosci Methods. 2003, 123(2):167-73.
[37] Dreesmann. L, Mittnacht. U, Lietz. M. et al. Nerve fibroblast impact on Schwann cell behavior[J]. Eur J Cell biol. 2009,88:285-300
[38] Gerburg. K, Hisham. F, Wolfgang. S et al. In vivo predegeneration of peripheral nerves: an effective technique to obtain activated Schwann cells for nerve conduits [J]. J Neurosci Methods, 1999,89:17-24
[39] Diany CM, Zayra G, Clara S, et al. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C) EJ]. J Neurosci Methods, 2002,114:1-8
[40]Levi. ADO. Characterization of the technique involved in isolating Schwann cells from adult human peripheral nerve [J]. J Neurosci Methods, 1996, 68: 21-26
[41]Haastert. K, Mauritz. C, Chaturvedi. S, et al. Human and rat adult Schwann cell cultures fast and efficient enrichment and highly effective non-viral transfection protocol[J]. Nat Protocols. 2007,2(1):99-104
[42] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[43] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000；16(3)：172-174
[44] 劳杰，熊良俭，赵新，等．不同时段预变神经雪旺细胞的形态学变化[J]．中华手外科杂志．2001：21(11)：689-691
[45] 劳杰，熊良俭，顾玉东，等．应用激活态的雪旺细胞填充导管修复周围神经缺损的初步研究[J]．中华手外科杂志．2000：16(4)：236-240
[46] 蒋良福，劳杰，何继银等．几丁糖胶原复合膜及激活态雪旺细胞修复周围神经缺损的实验研究[J]．中华手外科杂志，2005，21(3)：172-174
[47] Komiyama T, Nakao Y, Toyama Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit [J]. J Neurosci Methods, 2003, 122: 195-200.
[48]Yu QJ, Wei L, Tan HH, et al. Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment [J]. J Neurosci Methods, 2008, 170:140-148.
[49] Heldin NE, Paulsson Y, Forsberg K, et al. Inductionof cyclic AMP synthesis by forskolin is followed by a reduction in the expression of c-myc messenger RNA and inhibition of 3H-thymidine incorporation in human fibroblasts [J]. J Cell Physiol 1989, 138:17-23.
[50] Chang JY, Brown S. Cytosine arabinoside differentially alters survival and neurite outgrowth of neuronal PC12 cells [J]. Biochem Biophys Res Commun 1996, 218:753-8.
[51] Fields K, Raine C. Ultrastructure and immunocytochemistry of rat Schwann cells and fibroblasts in vitro [J]. J Neuroimmunol. 1982, 2:155-166.
[52] Maurice V, Norbert W. Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve [J]. J Neurosci Methods, 2003,124:135-143
[53] Paul. JK, Daniel. FK, Daljeet. M, et al. Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro [J]. Exp Neurol, 2007, 207: 267-274
[2] Nichols CM, Brenner MJ, Fox IK, et al. Effects of motor versus sensory nerve grafts on peripheral nerve regeneration [J]. Exp Neurol 2004; 190(2): 347-55.
[3] 劳杰，熊良俭，顾玉东，等．应用激活态的雪旺细胞填充导管修复周围神经缺损的初步研究[J]．中华手外科杂志．2000：16(4)：236-240
[4] Gregory. RD. Peripheral nerve injury: a review and approach to tissue engineered constructs [J]. Anat Rec. 2001, 263:396-404
[5] Choi BH, Zhu SJ, Kim SH, et al. Nerve repair using a vein graft filled with collagen gel [J]. J Reconstr Microsurg, 2005,21(4):267-72
[6] Mohammad. J, Shenaq. J, Rabinovsky. E, et al. Modulation of peripheral nerve regeneration: a tissue-engineering approach. The role of amnion tube nerve conduit across a 12 centimeter nerve gap [J]. Plast Reconstr Surg, 2000, 105 (2): 660-666.
[7] Geuna. S, Tos. P, Battiston. B, et al. Bridging peripheral nerve defects with muscle- vein combined guides [J]. Neurol Res, 2004, 26(2):139-44.
[8] Chenite. A, Chaput. C, Wang. D, et al. Novel injectable neutral solutions of chitosan form biodegradable gels in situ [J]. Biomaterials, 2000, 21: 2155-2161.
[9] Yoshii S, Shima M, Oka M, et al. Nerve regeneration along collagen filament and the presence of distal nerve stump [J]. Neurol Res, 2004, 26(2):145-50.
[10] Varon. S, Williams. IR. Peripheral nerve regeneration in a silicone model chamber: cellular and molecular aspects[J]. Peripheral Nerve Repair and Regeneration, 1986, 1: 9-15
[11] James. D, Arundathi. R, Les. O, et al. The Ability of Human Schwann Cell Grafts to Promote Regeneration in the Transected Nude Rat Spinal Cord[J]. Exp Neurol, 1997, 148: 502-522
[12] Fox. IK, Schwetye. KE, Keune. JD. Schwann cell injection of cold-preserved nerve allografts [J]. Microsurgery, 2005, 25(6):502-7.
[13] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells []]. J Neurosci Res, 2004, 77: 453-461.
[14] Paul. JK, Daniel. FK, Daljeet. M, et al. Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro [J]. Exp Neurol, 2007, 207:267-274
[15] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000：16(3)：172-174
[16] 劳杰，熊良俭，赵新，等．不同时段预变神经雪旺细胞的形态学变化[J]．中华手外科杂志．2001：21(11)：689-691
[17] 蒋良福，劳杰，何继银等．几丁糖胶原复合膜及激活态雪旺细胞修复周围神经缺损的实验研究[J]．中华手外科杂志，2005，21(3)：172-174
[18] 劳杰，蒋良福，顾玉东．激活态雪旺细胞生长相关蛋白43mRNA表达的研究[J]．中华骨科杂志，2005，25(6)：372-374
[19] 劳杰，蒋良福，顾玉东等，脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003 19(2)：109-111
[20] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002 18(2)：174-176
[21] 何继银，劳杰，蒋良福等，激活态雪旺细胞信号转导通路的初步研究[J]．中华手外科杂志，2006，22(6)：376-378
[1] 劳杰，熊良俭，顾玉东，等．改良成年SD大鼠雪旺细胞培养的实验研究．中华手外科杂志[J]，1999，15：106-108．
[2] Michael E. Pannunzio, I-ming J. A new method of selecting Schwann cells from adult mouse sciatic nerve [J]. J Neurosci Methods 2005, 149: 74-81
[3] Jirsova. K, Sodaar. P, Mandys. V. et al. Cold jet: a method to obtain pure Schwann cell cultures without the need for cytotoxic, apoptosis inducing drug treatment. J Neurosci Methods [J]1997,78:133-137
[4] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells [J]. J Neurosci Res, 2004, 77: 453-461.
[5] Gerburg. K, Hisham. F, Wolfgang. S et al. In vivo predegeneration of peripheral nerves an effective technique to obtain activated Schwann ceils for nerve conduits [J]. J Neurosci Methods, 1999,89:17-24
[6] Diany CM, Zayra G, Clara S, et al. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C) [J]. J neurosci Methods, 2002, 114: 1-8
[7] Yu QJ, Wei L, Tan HH, et al. Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment [J]. J neurosci Methods, 2008, 170:140-148.
[8] Heldin NE, Paulsson Y, Forsberg K, et al. Inductionof cyclic AMP synthesis by forskolin is followed by a reduction in the expression of c-myc messenger RNA and inhibition of 3H-thymidine incorporation in human fibroblasts [J]. J Cell Physiol 1989, 138:17-23.
[9] Chang JY, Brown S. Cytosine arabinoside differentially alters survival and neurite outgrowth of neuronal PC12 cells [J]. Biochem Biophys Res Commun 1996, 218: 753-8.
[10] Fields K, Raine C. Ultrastructure and immunocytochemistry of rat Schwann cells and fibroblasts in vitro [J]. J Neuroimmunol. 1982, 2: 155-166.
[11] Maurice V, Norbert W. Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve [J]. J Neurosci Methods, 2003,124:135-143
[12] Komiyama T, Nakao Y, Toyama Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit [J]. J Neurosci Methods, 2003, 122: 195-200.
[13] 胡晞棠，熊良俭，劳杰．从成年大白鼠坐骨神经分离培养许旺细胞的方法学探讨[J]．中华显微外科杂志，2002，25：197-200．
[14] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[15] Minghetti. L, Goodearl. ADJ, Mistry. K, et al. Glial growth factors Ⅰ-Ⅲ are specific mitogens for glial cells [J]. J Neurosci Res, 1996, 43:684-693.
[16]劳杰，姜良福，顾玉东，等．脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003，19：109-110．
[17]Haynes LW, Rushton JA, Perrins MF, et al. Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formation in vivo [J]. J Neurosci Methods 1994, 52:119-27.
[1] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[2] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000：16(3)：172-174
[3] 劳杰，熊良俭，赵新，等．不同时段预变神经雪旺细胞的形态学变化[J]．中华手外科杂志．2001：21(11)：689-691
[4] 劳杰，熊良俭，顾玉东，等．应用激活态的雪旺细胞填充导管修复周围神经缺损的初步研究[J]．中华手外科杂志．2000：16(4)：236-240
[5] 蒋良福，劳杰，何继银等．几丁糖胶原复合膜及激活态雪旺细胞修复周围神经缺损的实验研究[J]．中华手外科杂志，2005，21(3)：172-174
[6]劳杰，姜良福，顾玉东，等．脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003，19：109-110．
[7]Stafford. JM, Wilkinson. JC, Beechem. JM, et al. Accessory factors facilitate the binding of glucocorticoid receptor to the phosphoenolpyruvate carboxykinase gene promoter[J]. J Biol Chem. 2001, 276(43): 39885-39891.
[8]Raltreider. RC, Davis. AM, lariviere. JP. et al. Arsenic alters the function of the glucocorticoid receptor as a transcription factor[J]. Environ Health Perspect. 2001, 109(3):245-251.
[9]Scott. DK, Stromsted. PE, Wang. JC, et al. Further characterization of the glucocorticoid response unit in the phosphoenolpyruvate carboxykinase gene. The role of the glucocorticoid receptor-binding sites[J]. Mol. Endo. 1998, 12(4):482-491.
[10]Imai. E, Miner. JN, Mitchell. JA, et al. Glucocorticoid receptor-cAMP response element-binding protein interaction and the response of the phosphoenolpyruvate carboxykinase gene to glucocorticoids[J]. J Biol Chem. 1993, 268(8): 5353-5356.
[11]Marhenke. S, Lamle. J, Buitrago-Molina. LE, et al. A novel role of the NRF2 transcription factor in the regulation of arsenite-mediated keratin 16 gene expression in human keratinocytes[J]. Hepatology. 2008,48(2): 487-496.
[12]Williams. MA, Rangasamy. r, Bauer. SM, et al. Disruption of the transcription factor Nrf2 promotes pro-oxidative dendritic cells that stimulate Th2-like immunoresponsiveness upon activation by ambient particulate matter[J]. J Immuno. 2008,181:4545-4559.
[13]Alam. J, Cook. JL. How many transcription factors does it take to turn on the heme oxygenase-1 gene[J]. Am J Respir Cell Mol Biol. 2007, 36:166-174.
[14]Huang. A, Zhang. YY, Chen.K. et al. Cytokine-stimulated GTP cyclohydrolase I expression in endothelial cells requires coordinated activation of nuclear factor-kappaB and Statl Stat3[J]. Circ Res.2005,96:164-171.
[15]Legeendre. F, Dudhia. J, Pujol.JP. et al. JAK STAT but not ERK1 ERK2 pathway mediates interleukin (IL) -6 soluble IL-6R down-regulation of Type Ⅱ collagen[J]. J Biol Chem. 2003, 278(5):2903-2912.
[16]Schumann. G, Huell.M, Machein.U. et al. Interleukin-6 activates signal transducer and activator of transcription and mitogen-activated protein kinase signal transduction pathways and induces de novo protein synthesis in human neuronal cells [J]. J Neurochem. 1999,73:2009-2017.
[17]Niehof.M, Streetz. K, Rakemann. T, et al. Interleukin-6-induced tethering of STAT3 to the LAP C EBPbeta promoter suggests a new mechanism of transcriptional regulation by STAT3 [J]. J Biol Chem. 2001, 276(12):9016-9027.
[18]Colin. Y, Joulin.V, Kim. CLV. et al. Characterization of a new erythroid megakaryocyte-specific nuclear factor that binds the promoter of the housekeeping human glycophorin C gene[J]. J Biol Chem. 1990,265(28):16729-16732.
[19]Profita.M, Bonanno. A, Siena.L. et al. Acetylcholine mediates the release of IL—8 in human bronchial epithelial cells by a NFkB ERK-dependent mechanism[J]. Eur J Pharmacol. 2008,582:145-153.
[20]Novotny.NM, Markel.TA, Crisostomo.PR, et al. Differential IL-6 and VEGF secretion in adult and neonatal mesenchymal stem cells role of NFkB[J]. Cytokine. 2008,43:215-219.
[2l]Grieder.NC, Nellen.D, Burke. R, et al. Schnurri is required for Drosophila Dpp signaling and encodes a zinc finger protein similar to the mammalian transcription factor PRDII-BF1[J]. Cell. 1995, 81:791-800.
[22]Muchardt.C, Seeler. JS, Nirula. A, et al. Regulation of human immunodeficiency virus enhancer function by PRDII-BF1 and c-rel gene products[J]. J Virol. 1992, 66(1): 244-250.
[23]Lin. R, Gewert. D, Hiscott.J. Differential transcriptional activation in vitro by NF-kappa B Rel proteins[J]. J Biol Chem. 1995,270(7): 3123-3131.
[1]Wang. JC, Derynck. MK, Nonaka. DF et al. Chromatin immunoprecipitation (ChIP) scanning identifies primary Glucocorticoid receptor target genes[J]. Proc. Natl. Acad. Sci. 2004, 101(44):15603-15608
[2]Kondo.Y, Shen. L, Yan. PS, et al. Chromatin immunoprecipitation microarrays for identification of genes silenced by histone H3 lysine 9 methylation[J]. Proc. Natl. Acad. Sci. 2004, 101(19):7398-7403
[3]Lavelle. D, Vaitkus.K, Hankewych. M, et al. Developmental changes in DNA methylation and covalent histone modifications of chromatin associated with the ε-, γ -, and β-globin gene promoters in Papio anubis[J]. Blood cells, Molecules, and diseases. 2006,36:269-278.
[4]Lavelle. D, Vaitkus.K, Hankewych. M, et al. Effect of 5-aza-2 '-deoxycytidine (Dacogen) on covalent histone modifications of chromatin associated with the -, -, and β-globin promoters in Papio anubis[J].Exp Hematol. 2006,34:339-347.
[5]Dann.SG, Allison.T, Veldhoen. K, et al. Chromatin immunoprecipitation assay on the rainbow trout opsin proximal promoters illustrates binding of NF- κ B and c-jun to the SWS1 promoter in the retina[J]. Exp Eye Res.2004,78: 1015-1024
[6]Weber. M, Davies. JJ, Wittig. D, et al. Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells[J]. Nat Genet.2005, 37 (8): 853-862.
[7]Buck. MJ, Lieb. JD. ChIP-chip: considerations for the design analysis and application of genome-wide chromatin immunoprecipitation experiments[J]. Genomics. 2004,83:349-360.
[8]Arie. NB, McCll.AE, Berkman. s, et al. Evolutionary conservation of sequence and expression of the bHLH protein Atonal suggests a conserved role in neurogenesis[J]. Human Mol Genet. 1996, 5(9):1207-1216.
[9]Seipel.K, Yanze.N, Schmid. V. Developmental and evolutionary aspects of the basic helix-loop-helix transcription factors Atonal-like 1 and Achaete-scute homolog 2 in the jellyfish[J]. Dev Biol. 2004, 269: 331 -345.
[10]Briggs. KJ, Schwartz. IM, Zhang. W, et al. Cooperation between the Hicl and Ptchl tumor suppressors in medulloblastoma[J]. Gen Dev. 2008,22:770-785.
[11]Lin. W, Kemper. A, McCarthy, Et al. Interferon-gamma induced medulloblastoma in the developing cerebellum [J]. JNeurosci. 2004,24(45):10074-10083.
[12]Vilar. M, Murillo-Carretero. M, Mira.H, et al. Bexl, a novel interactor of the p75 neurotrophin receptor, links neurotrophin signaling to the cell cycle[J]. EMBO. 2006, 25:1219-1230.
[13] Koo. JH, Saraswati.M, Margolis.FL. Immunolocalization of Bex protein in the mouse brain and olfactory system[J]. J Comp Neurol. 2005, 487:1-14.
[14]Koo. JH, Smiley. MA, Lovering. RM, et al. Bexl knock out mice show altered skeletal muscle regeneration[J]. Biochem Biophys Res Commun.2007,363:405-410.
[15]Alvarez.E, Zhou.W, Witta. SE, et al. Characterization of the Bex gene family in humans, mice, and rats[J]. Gene. 2005,357:18-28.
[16]Nystedt. S, Emilsson. K, Wahlestedt.C, et al. Molecular cloning of a potential proteinase activated receptor [J]. Proc Natl Acad Sci. 1994, 91:9208-9212.
[17]01ianas. MC, Dedoni.S, Onali.P. Proteinase-activated receptors 1 and 2 in rat olfactory system: layer-specific regulation of multiple signaling pathways in the main olfactory bulb and induction of neurite retraction in olfactory sensory neurons [J]. Neurosci. 2007, 146:1289-1301.
[18]Costa. R, Marotta. DM, Manjavachi.MN. et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice[J]. Br J Pharmacol. 2008,154:1094-1103.
[19]Luo. WB, Wang. YF, Reiser. G. Protease-activated receptors in the brain receptor expression, activation, and functions in neurodegeneration and neuroprotection[J]. Brain Res Rev. 2007, 56: 331-345.
[20] Wang. YF, Luo. WB, Reiser.G. Activation of protease-activated receptors in astrocytes evokes a novel neuroprotective pathway through release of chemokines of the cytokine-induced neutrophil chemoattractant family[J]. Eur J Neurosci. 2007, 25: 3159-3168.
[21]Park. GH, Ryu. JR, Shin. CY, et al. Evidence that protease-activated receptor-2 mediates trypsin-induced reversal of stellation in cultured rat astrocytes[J]. Neurosci Res. 2006, 54: 15-23.
[22]Taanman. JW, Rahman. S, Pagnamenta. AT, et al. Analysis of mutant DNA polymerase gamma in patients with mitochondrial DNA depletion[J].Hum Murat. 2009, 30: 248-254.
[23]Ueta. E, Sasabe. E, Yang. Z, et al. Enhancement of apoptotic damage of squamous cell carcinoma cells by inhibition of the mitochondrial DNA repairing system[J]. Cancer Sci. 2008, 99(11):2230-2237.
[24] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000：16(3)：172-174
[25] 劳杰，蒋良福，顾玉东．激活态雪旺细胞生长相关蛋白43mRNA表达的研究[J]．中华骨科杂志，2005，25(6)：372-374
[26] 劳杰，蒋良福，顾玉东等，脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003 19(2)：109-111
[27]Facchin. F, Canaider. S, Vitale. L, et al. Identification and analysis of human RCAN3 (DSCR1L2) mRNA and protein isoforms[J].Gene. 2008, 407: 159-168.
[28]Porta. S, Marti. E, Luna. S, et al. Differential expression of members of the RCAN family of calcineurin regulators suggests selective functions for these proteins in the brain[J]. Eur J Neurosci. 2007,26:1213-1226.
[29]Rodriguez. P. Oligodendrocyte development and thyroid hormone[J].J Neurobiol. 1999, 40: 497-512.
[1] 劳杰，熊良俭，顾玉东，等．改良成年SD大鼠雪旺细胞培养的实验研究[J]．中华手外科杂志，1999，15：106-108．
[2] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells[J]. J Neurosci Res, 2004, 77: 453-461.
[3] Diany CM, Zayra G, Clara S, et al. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C) [J]. J Neurosci Methods, 2002,114:1-8
[4] Maurice V, Norbert W. Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve [J]. J Neurosci Methods, 2003,124:135-143
[5] Yu QJ, Wei L, Tan HH, et al. Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment [J]. J Neurosci Methods, 2008, 170:140-148.
[6] Komiyama T, Nakao Y, Ioyama Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit [J]. J Neurosci Methods, 2003, 122: 195-200.
[7] 胡晞棠，熊良俭，劳杰．从成年大白鼠坐骨神经分离培养许旺细胞的方法学探讨[J]．中华显微外科杂志，2002，25：197-200．
[8] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[9] 劳杰，姜良福，顾玉东，等．脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究[J]．中华手外科杂志，2003，19：109-110．
[1] RaviV. B . Peripheral nerve regeneration: an opinion on channels,scaffolds and anisotropy [J]. Biomaterials. 2006, 27: 3515-3518
[2] Sunderland S. Rate of regeneration of I : sensory nerve fibres and II: motor fibres [J]. Arch Neurol Psychiatry, 1947, 58:1 - 14.
[3] MacKinnon SE. New directions in peripheral nerve surgery [J]. Ann Plast Surg, 1989, 22 (3) :257 - 273.
[4] Mackinnon. SE, Dellon. AL. Anatomy and physiology of the peripheral nerve. Surgery of the peripheral nerve [M]. New York: Thieme, 1988:1 -33.
[5] Nichols CM, Brenner MJ, Fox IK, et al. Effects of motor versus sensory nerve grafts on peripheral nerve regeneration [J]. Exp Neurol 2004;190(2) :347-55.
[6] Millesi. H, Meissl. G, Berger. A. Further experience with interfascicular grafting of the median, ulnar and radial nerves [J]. J Bone Joint Surg. 1976, 58: 209-218.
[7] Rutkowski. GE, Heath. CA. Development of a Bioartificial Nerve Graft. Design Based on a Reaction-Diffusion Model [J]. Biotechnol.Prog. 2002; 18:362-372
[8] Rutkowski. GE, Heath. CA. Development of a Bioartificial Nerve Graft.Nerve Regeneration in vitro Biotechnol[J]. Prog. 2002; 18:373-379
[9] Hu M, Sabelman. EE, Tsai. C, et al. Improvement of Schwann Cell Attachment and proliferation on Modified Hyaluronic Acid Strands by Polylysine [J]. Tissue Eng, 2000; 6(6):585-593
[10] Hadlock. T, Sundback. C, Hunter. D, et al. A polymer Conduit seeds with Schwann Cells Promotes Guided Peripheral Nerve Regeneration [J].Tissue Eng, 2000; 6(2):119-127
[11] Fox.IK, Schwetye. KE, Keune. JD. Schwann cell injection of cold-preserved nerve allografts [J]. Microsurgery, 2005, 25(6):502-7.
[12] Mosahebi. A, Fuller. P, Wiberg. M, et al. Effect of allogeneic Schwann cell transplantation on peripheral nerve regeneration [J]. Exp Neurol,2002,173(2): 213-23.
[13]Fansa. H, Dodic. T, Wolf. G, et al. Tissue engineering of peripheral nerves Epineurial grafts with application of cultured Schwann cells [J].Microsurgery, 2003, 23: 72-77
[14] Schlosshauer. B, Muller. E, Schroder. B et al. Rat Schwann cells in bioresorbable nerve guides to promote and accelerate axonal regeneration [J]. Brain Res, 2003, 963: 321-326.
[15] Frerichs. 0, Fansa. H, Schicht.C. et al. Reconstruction of peripheral nerves using acellular nerve grafts with implanted cultured Schwann cells[J]. Microsurgery, 2002, 22: 311-315
[16] Rodriquez.FT, Verdu. E, Ceballos. D. Nerve guides seeded with autologous Schwann cells improve nerve regeneration[J ]. Exp Neurol.2000; 161:571—84.
[17] Lundborg G, Dahlin LB. Reorganisation and orientation of regeneration nerve fibers , perineurium , and epineurium in preformed mesothelial bubes-an experimental study on the sciatic nerve of rats[J ] . J Neurosci Res, 1981, 6 :256 - 281.
[18] Choi BH, Zhu SJ, Kim SH, et al. Nerve repair using a vein graft filled with collagen gel [J]. J Reconstr Microsurg, 2005, 21(4):267-72
[19] Mohammad. J, Shenaq. J, Rabinovsky. E, et al. Modulation of peripheral nerve regeneration: a tissue-engineering approach. The role of amnion tube nerve conduit across a 12 centimeter nerve gap [J]. Plast Reconstr Surg, 2000, 105 (2): 660-666.
[20]Geuna. S, Tos. P, Battiston. B, et al. Bridging peripheral nerve defects with muscle- vein combined guides [J]. Neurol Res. 2004,26(2): 139-44.
[21]Keilhoff. G, Pratsch. F, Wolf. G, et al. Bridging extra large defects of peripheral nerves: possibilities and limitations of alternative biological grafts from acellular muscle and Schwann cells [J]. Tissue Eng,2005; 11(7):1004-1014
[22] Norris R W, Glasby M A. Peripheral nerx re repair in numaus using muscle autografts[J]. J Bone and Joint Surg, 1988,70:530-537
[23]Mohammad. J, Shenaq. J, Rabinovsky. E,. et al . Modulation of peripheral nerve regeneration: a tissue-engineering approach. The role of amnion tube nerve conduit across a 12centimeter nerve gap [J]. Plast Reconstr Surg, 2000, 105 (2): 660-666.
[24]Liu. W, Cui.L, Cao.YL. A closer view of tissue engineering in China: the experience of tissue construction in immunocompetent animals[J]. Tissue Eng, 2003; 9(s1):17-31
[25]Yuan. Y, Zhang. P, Yang. YM, et al. The interaction of Schwann cells with chitosan membranes and fibers in vitro[J]. Biomaterials. 2004, 25: 4273-4278
[26]Wei. X, Lao. J, Gu. YD. Bridging peripheral nerve defect with chitosan-collagen film[J].Chin J Traumatol. 2003, 6(3):131-4.
[27]Varon S, William s IR. Peripheral nerve regeneration in a silicone model chamber:cellular and molecular aspects[J]. Peripheral Nerve Repair and Regeneration, 1986, 1:9-15
[28]Ceonzo K, Gaynor A, Shaffer L, Polyglycolic Acid-Induced Inflammation: Role of Hydrolysis and Resulting Complement Activation [J]. Tissue Eng, 2006, 12(2):301-308.
[29]Richard. B. Cellular engineering: molecular repair of membranes to rescue cells of the damaged nervous system [J]. Neurosurgery. 2001, 49(2):370-379
[30]Wells. MR, Kraus. K, Batter. DK, et al. Gel matrix vehicles for growth factor application in nerve gap injurys repaired with tube: a comparison of biomatrix, collagen, and methylcellulose. Exp Neurol. 1997, 146: 395-402
[31] 何继银，劳杰．周围神经损伤后雪旺细胞生存与凋亡的动态平衡[J]．中华创伤骨科杂志，2004 6(3)：330-333
[32]Popovic. M, Sketelj. J, Bresjanac. M. Changes of Schwann cell antigenic profile after peripheral nerve injury[J]. Eur J Physiol. 1996,431:287-288
[33]Singh. N, Birdi. TJ, Chandrashekar. S, et al. Schwann cell extracellular matrix protein production is modulated by Mycobacterium leprae and macrophage secretory products [J]. J Neurol SCi, 1997, 151:13-22.
[34] Tada H, Hatoko M, Tanaka A, et al. Detection of E-cadherin expression after nerve repair in a rat Sciatic nerve model[J]. Ann Plast Surg, 2001, 47: 178-182.
[35] Christina M, Claudia G, Kirsten H. Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells [J]. J Neurosci Res, 2004, 77: 453-461.
[36]Manent. J, Oguievetskaia. K, Bayer. J, et al. Magnetic cell sorting for enriching Schwann cells from adult mouse peripheral nerves [J]. J Neurosci Methods. 2003, 123(2):167-73.
[37] Dreesmann. L, Mittnacht. U, Lietz. M. et al. Nerve fibroblast impact on Schwann cell behavior[J]. Eur J Cell biol. 2009,88:285-300
[38] Gerburg. K, Hisham. F, Wolfgang. S et al. In vivo predegeneration of peripheral nerves: an effective technique to obtain activated Schwann cells for nerve conduits [J]. J Neurosci Methods, 1999,89:17-24
[39] Diany CM, Zayra G, Clara S, et al. Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C) EJ]. J Neurosci Methods, 2002,114:1-8
[40]Levi. ADO. Characterization of the technique involved in isolating Schwann cells from adult human peripheral nerve [J]. J Neurosci Methods, 1996, 68: 21-26
[41]Haastert. K, Mauritz. C, Chaturvedi. S, et al. Human and rat adult Schwann cell cultures fast and efficient enrichment and highly effective non-viral transfection protocol[J]. Nat Protocols. 2007,2(1):99-104
[42] 劳杰，顾玉东．激活态雪旺细胞在细胞培养下的生长规律[J]．中华手外科杂志，2002，18：174-176．
[43] 劳杰，熊良俭，顾玉东，等．不同时段的预变性神经段分子生物学变化[J]．中华手外科杂志．2000；16(3)：172-174
[44] 劳杰，熊良俭，赵新，等．不同时段预变神经雪旺细胞的形态学变化[J]．中华手外科杂志．2001：21(11)：689-691
[45] 劳杰，熊良俭，顾玉东，等．应用激活态的雪旺细胞填充导管修复周围神经缺损的初步研究[J]．中华手外科杂志．2000：16(4)：236-240
[46] 蒋良福，劳杰，何继银等．几丁糖胶原复合膜及激活态雪旺细胞修复周围神经缺损的实验研究[J]．中华手外科杂志，2005，21(3)：172-174
[47] Komiyama T, Nakao Y, Toyama Y, et al. A novel technique to isolate adult Schwann cells for an artificial nerve conduit [J]. J Neurosci Methods, 2003, 122: 195-200.
[48]Yu QJ, Wei L, Tan HH, et al. Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment [J]. J Neurosci Methods, 2008, 170:140-148.
[49] Heldin NE, Paulsson Y, Forsberg K, et al. Inductionof cyclic AMP synthesis by forskolin is followed by a reduction in the expression of c-myc messenger RNA and inhibition of 3H-thymidine incorporation in human fibroblasts [J]. J Cell Physiol 1989, 138:17-23.
[50] Chang JY, Brown S. Cytosine arabinoside differentially alters survival and neurite outgrowth of neuronal PC12 cells [J]. Biochem Biophys Res Commun 1996, 218:753-8.
[51] Fields K, Raine C. Ultrastructure and immunocytochemistry of rat Schwann cells and fibroblasts in vitro [J]. J Neuroimmunol. 1982, 2:155-166.
[52] Maurice V, Norbert W. Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve [J]. J Neurosci Methods, 2003,124:135-143
[53] Paul. JK, Daniel. FK, Daljeet. M, et al. Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro [J]. Exp Neurol, 2007, 207: 267-274