自旋捕捉剂修饰及生物学效应研究
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摘要
本文通过对自旋捕捉剂结构优化,研究其在细胞体系的应用。
本文主要的研究进展
1、借助gaussian,优化分子结构,比较新型自旋捕捉剂反应前后键级、键长、二面角、前线轨道等参数,解释了为何间位取代的PBN加快能较快和甲基自由基反应。并通过比较各种自旋捕捉剂不同自由基自旋加合物的热力学能量,反应焓变,生成热,确定了较稳定的自旋捕捉剂结构,并通过实验验证了这种结构的稳定性。
2、电子顺磁共振(ESR)技术能够检测自旋捕捉剂自由基加合物的信号。但检测细胞内的自由基信号鲜有报道。本文利用ESR技术检测,用自旋捕捉剂BMPO捕捉了肿瘤细胞HepG2内的自由基,并通过模拟及实验的方法验证了该信号为超氧阴离子信号。
3、本文利用还原性荧光探针和氧化性荧光探针分别比较了肿瘤细胞HepG2和正常细胞L02两种细胞株的氧化还原水平,发现在HepG2细胞内的氧化性水平比L02细胞株高,而且还原水平HepG2细胞也同样要高。
This article bases on the spin trapping agents optimization and its application in cell lines.
In this paper, the research is as follows
1. With gaussian, optimizing spin trappers'molecular structure is to compare the spin trappers and their adducts agent bond, bond length, enthalpy, diangle, frontier orbital parameters, to explain why derivative which is meta-position substituted is more reactive with methyl radical. and. Then comparing the variety of different free radical spin trapping agents'thermodynamic energy of the spin adduct, the reaction enthalpy is to determine a more stable structure among spin trapping agents, which is validated through experiments.
2. ESR technique can effectively detect the adducts'signal of spin trapper and free radical. But the natural detection of intracellular free radical is rarely reported. In this paper, a spin trapping agent BMPO captures tumor cells HepG2'free radical which is detected by ESR, and simulation and experimental comfirm that the signal is belong to the oxide-center and carbon-center free radical adducts.
3. In this paper, redux fluorescent probes and oxidize fluorescent probe were used to compare the tumor cells line HepG2 and normal cell line L02's redox level,finding that in HepG2 cells the level of oxidation is over that of L02 cells, and reducing state level of HepG2 cells is also higher.
本文主要的研究进展
1、借助gaussian,优化分子结构,比较新型自旋捕捉剂反应前后键级、键长、二面角、前线轨道等参数,解释了为何间位取代的PBN加快能较快和甲基自由基反应。并通过比较各种自旋捕捉剂不同自由基自旋加合物的热力学能量,反应焓变,生成热,确定了较稳定的自旋捕捉剂结构,并通过实验验证了这种结构的稳定性。
2、电子顺磁共振(ESR)技术能够检测自旋捕捉剂自由基加合物的信号。但检测细胞内的自由基信号鲜有报道。本文利用ESR技术检测,用自旋捕捉剂BMPO捕捉了肿瘤细胞HepG2内的自由基,并通过模拟及实验的方法验证了该信号为超氧阴离子信号。
3、本文利用还原性荧光探针和氧化性荧光探针分别比较了肿瘤细胞HepG2和正常细胞L02两种细胞株的氧化还原水平,发现在HepG2细胞内的氧化性水平比L02细胞株高,而且还原水平HepG2细胞也同样要高。
This article bases on the spin trapping agents optimization and its application in cell lines.
In this paper, the research is as follows
1. With gaussian, optimizing spin trappers'molecular structure is to compare the spin trappers and their adducts agent bond, bond length, enthalpy, diangle, frontier orbital parameters, to explain why derivative which is meta-position substituted is more reactive with methyl radical. and. Then comparing the variety of different free radical spin trapping agents'thermodynamic energy of the spin adduct, the reaction enthalpy is to determine a more stable structure among spin trapping agents, which is validated through experiments.
2. ESR technique can effectively detect the adducts'signal of spin trapper and free radical. But the natural detection of intracellular free radical is rarely reported. In this paper, a spin trapping agent BMPO captures tumor cells HepG2'free radical which is detected by ESR, and simulation and experimental comfirm that the signal is belong to the oxide-center and carbon-center free radical adducts.
3. In this paper, redux fluorescent probes and oxidize fluorescent probe were used to compare the tumor cells line HepG2 and normal cell line L02's redox level,finding that in HepG2 cells the level of oxidation is over that of L02 cells, and reducing state level of HepG2 cells is also higher.
引文
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