应用SRAP分子标记构建长果种黄麻遗传连锁图谱的研究
|
摘要
黄麻是天然的纤维作物,在国计民生中具有十分重要的地位。然而其在构建遗传连锁图谱及利用分子标记辅助育种方面,明显滞后于其他主要经济作物。目前,国内尚未有关长果黄麻遗传连锁图谱构建的报道。本研究利用SRAP分子标记构建国内首张长果种黄麻遗传连锁图谱,为长果种黄麻某些重要农艺性状的基因定位、QTL分析及分子标记辅助育种奠定了坚实的理论基础。本研究取得了以下的研究成果:
(1)以甜麻(长果野生种)和宽叶长果(长果栽培种)为亲本,杂交产生F1代,F1自交产生的F2代分离群体(187个单株),作为构图群体。
(2)以甜麻和宽叶长果为模板,从19X27对SRAP上游与下游引物组合中筛选出多态性较好的56对SRAP引物组合。
(3)利用筛选出的56对SRAP引物组合,对F2作图群体进行PCR扩增,共产生155个多态条带,平均每个引物组合产生2.8个多态条带,最多的可以产生6条。
(4)使用Mapmaker/exp 3.0,我们构建了包括125个SRAP标记位点的长果种黄麻的遗传连锁图谱,并对长果种黄麻重要质量性状基因进行定位和标记,这些标记被分为10个连锁群(LOD≥3.0),每对标记组合可以产生8~20个大小在100~2000bp清晰可辨的条带,每个连锁群有2~38个标记位点,总长为2231.9cM,两个标记间平均图距为17.86 cM。标记间最大间距为36.6cM,最小间距为4.5cM。标记在整个连锁群中分布比较均匀,未出现标记聚集的现象。
(5)质量性状定位:在本图谱中有3个形态标记,分别为托叶色、叶柄色和叶缘色。三者均位于构建的连锁群LG1上,托叶色基因与M18E7及M12E18连锁,遗传距离分别为11.2cM和12cM;叶柄色基因与M17E10及叶缘色基因连锁,遗传距离分别为21.1cM和4.5cM;叶缘色基因还与M12E9-1连锁,遗传距离为20.9cM。
(6)在这155个标记位点当中,有37个(23.9%)偏离3:1的分离比,偏分离比较严重。其中有28个(75.7%)偏向父本(甜麻)带型,其余9个(24.3%)偏向母本(宽叶长果)带型,可能是由配子的选择性引起的。长果种黄麻有7对染色体,而我们构建出10个连锁群,接近染色体数。本研究的平均图距还较大,有待于进一步深化,以期构建出一张标记分布均匀,位点密度较高的长果种黄麻遗传连锁图谱。
Jute(Corohorus olitorius L)is a nature fiber crop and plays a very important position in people′s livelihood. However, in the construction of genetic linkage map and the use of molecular marker-assisted breeding, it drops behind the other major crops. By now, there is no relative report on genetic linkage map of jute. In this study, SRAP markers were applied to construct the first jute genetic linkage map in country, which laid a solid foundation for the theory of agricultural traits of gene mapping, QTL analysis, molecular marker-assisted breeding. The major results as below:
(1) The 187 F2 individuals are from the cross of two jute, glyc-anesthesia (wild species) and wide leaf jute (cultivated species).
(2) Take glyc-anesthesia (wild species) and wide leaf jute (cultivated species) as template, 56 pairs of SRAP primer combinations with good polymorphism were selected from 19X27 forward and reverse of the SRAP primer combinations.
(3) Using the screened 56 pairs of SRAP primer combinations, PCR was applied on the F2 population. 155 polymorphic bands were generated in the F2 population analysis. The average of polymorphic bands produced by one primer pair was 2.8, and the maximum was 6.
(4) Using mapmaker/exp 3.0, a linkage map was constructed, which consisted of 125 SRAP markers distributed in ten linkage groups (L0D≥3 0). Each SRAP primer pair could generate 8~20 clear bands with a length range between 100 and 2000 bp.While each linkage group have 2~38 markers, 2231.9cM in total. The average map distance is 17.86cM, with the maximum map distance is 36.6cM and the minimum map distance is 4.5cM. The markers are well-distributed with aggregation in each linkage group.
(5) Position of qualitative traint:There were 3 morphological markers in the map.They were the colour of stipule,the colour of stipe and the colour of leaf margin. They were located in the linkage group 1.The colour of stipule linkaged with M18E7 and M12E18. The genetic distance between them are 11.2cM and 12 cM. The colour of stipe linkaged with M17E10 and the colour of leaf margin.The genetic distance are 21.1cM and 4.5cM.The colour of leaf margin linkaged with M12E9-1 and the genetic distance is 20.9cM.
(6) Among these 155 markers,37 markers (23.9%) segregated unconventionally against the expected 3:1 ratio.28 (75.7%) with the type of male parent, and the other 9 (24.3%) preferred the type of female parent, which could be caused by selectivity of gametes. Although jute has 7 pairs of chromosomes,we constructed ten linkage groups, which close to the chromosome number. In this study,the average map distance is too large, which requires to be further deepened for building a genetic linkage map of Jute with evenly-distributed markers and high-density sites.
(1)以甜麻(长果野生种)和宽叶长果(长果栽培种)为亲本,杂交产生F1代,F1自交产生的F2代分离群体(187个单株),作为构图群体。
(2)以甜麻和宽叶长果为模板,从19X27对SRAP上游与下游引物组合中筛选出多态性较好的56对SRAP引物组合。
(3)利用筛选出的56对SRAP引物组合,对F2作图群体进行PCR扩增,共产生155个多态条带,平均每个引物组合产生2.8个多态条带,最多的可以产生6条。
(4)使用Mapmaker/exp 3.0,我们构建了包括125个SRAP标记位点的长果种黄麻的遗传连锁图谱,并对长果种黄麻重要质量性状基因进行定位和标记,这些标记被分为10个连锁群(LOD≥3.0),每对标记组合可以产生8~20个大小在100~2000bp清晰可辨的条带,每个连锁群有2~38个标记位点,总长为2231.9cM,两个标记间平均图距为17.86 cM。标记间最大间距为36.6cM,最小间距为4.5cM。标记在整个连锁群中分布比较均匀,未出现标记聚集的现象。
(5)质量性状定位:在本图谱中有3个形态标记,分别为托叶色、叶柄色和叶缘色。三者均位于构建的连锁群LG1上,托叶色基因与M18E7及M12E18连锁,遗传距离分别为11.2cM和12cM;叶柄色基因与M17E10及叶缘色基因连锁,遗传距离分别为21.1cM和4.5cM;叶缘色基因还与M12E9-1连锁,遗传距离为20.9cM。
(6)在这155个标记位点当中,有37个(23.9%)偏离3:1的分离比,偏分离比较严重。其中有28个(75.7%)偏向父本(甜麻)带型,其余9个(24.3%)偏向母本(宽叶长果)带型,可能是由配子的选择性引起的。长果种黄麻有7对染色体,而我们构建出10个连锁群,接近染色体数。本研究的平均图距还较大,有待于进一步深化,以期构建出一张标记分布均匀,位点密度较高的长果种黄麻遗传连锁图谱。
Jute(Corohorus olitorius L)is a nature fiber crop and plays a very important position in people′s livelihood. However, in the construction of genetic linkage map and the use of molecular marker-assisted breeding, it drops behind the other major crops. By now, there is no relative report on genetic linkage map of jute. In this study, SRAP markers were applied to construct the first jute genetic linkage map in country, which laid a solid foundation for the theory of agricultural traits of gene mapping, QTL analysis, molecular marker-assisted breeding. The major results as below:
(1) The 187 F2 individuals are from the cross of two jute, glyc-anesthesia (wild species) and wide leaf jute (cultivated species).
(2) Take glyc-anesthesia (wild species) and wide leaf jute (cultivated species) as template, 56 pairs of SRAP primer combinations with good polymorphism were selected from 19X27 forward and reverse of the SRAP primer combinations.
(3) Using the screened 56 pairs of SRAP primer combinations, PCR was applied on the F2 population. 155 polymorphic bands were generated in the F2 population analysis. The average of polymorphic bands produced by one primer pair was 2.8, and the maximum was 6.
(4) Using mapmaker/exp 3.0, a linkage map was constructed, which consisted of 125 SRAP markers distributed in ten linkage groups (L0D≥3 0). Each SRAP primer pair could generate 8~20 clear bands with a length range between 100 and 2000 bp.While each linkage group have 2~38 markers, 2231.9cM in total. The average map distance is 17.86cM, with the maximum map distance is 36.6cM and the minimum map distance is 4.5cM. The markers are well-distributed with aggregation in each linkage group.
(5) Position of qualitative traint:There were 3 morphological markers in the map.They were the colour of stipule,the colour of stipe and the colour of leaf margin. They were located in the linkage group 1.The colour of stipule linkaged with M18E7 and M12E18. The genetic distance between them are 11.2cM and 12 cM. The colour of stipe linkaged with M17E10 and the colour of leaf margin.The genetic distance are 21.1cM and 4.5cM.The colour of leaf margin linkaged with M12E9-1 and the genetic distance is 20.9cM.
(6) Among these 155 markers,37 markers (23.9%) segregated unconventionally against the expected 3:1 ratio.28 (75.7%) with the type of male parent, and the other 9 (24.3%) preferred the type of female parent, which could be caused by selectivity of gametes. Although jute has 7 pairs of chromosomes,we constructed ten linkage groups, which close to the chromosome number. In this study,the average map distance is too large, which requires to be further deepened for building a genetic linkage map of Jute with evenly-distributed markers and high-density sites.
引文
[1]祁建民,刘国忠著.黄麻红麻品种与高效配套技术[J].台湾出版社,2007:1-12.
[2]中国农业科学院麻类研究所.中国黄麻红麻品种志[M].农业出版社,1985:10.
[3]张泉.关于植物形态学的思考[J].自然杂志,2002(3):180-183.
[4]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].科学出版社,2000:2.
[5]方嘉禾,常汝镇著.中国作物及其野生近缘植物——经济作物卷[M].中国农业出版社,2007:305-307.
[6]祁建民,李维明,吴为人.黄麻的起源与演化[J].作物学报,1997,23(6):67-68.
[7]胡立勇,丁艳锋主编.作物栽培学[M].高等教育出版社,北京,2009:12.
[8]熊和平主编.麻类作物育种学[M].中国农业科学技术出版社,2008(2):156.
[9]中国农业科学院麻类研究所主编.中国麻类作物栽培学[M].农业出版社,1982:123.
[10]Kundu B C, Jute Worlds Formost, Bast Fibers, et.al. Diseases and pests[J].Economic Botany,1956a, 10(2):103-133.
[11] R.L.史密斯, 1977,李建东等译, 1988.生物学原理和野外生物学[M].科学出版社,北京,14-20.
[12]张金泉,植物地理学[M].重庆出版社,上海, 1989, 224-228.
[13] H.N.瓦维洛夫,1935,许运天译,栽培植物的起源中心[M].农业出版社,北京,1982:31.
[14] Kundu B.C. The Active Principles of corchorus olitorius L(Jute) Leaves[J]. Sciences and Culture, 1951:16.
[15] De Condolle, 1882,俞德浚、蔡希陶译, 1940,农艺植物考源[M].上海:商务印书馆,147-150.
[16] Jennifer M Edmonds.Herbarium survey of Cofchorus L. species[J]. Department of Plant Sciences, University of Oxford,1-2.
[17]李宗道.麻作的理论与技术[M].上海科技出版社,1980:388-390.
[18]卢浩然.中国农百科全书——农作物卷,麻类分支[M].农业出版社,北京,1991:256.
[19]李德芳.红/黄麻新用途综合开发与国际合作[J].中国麻业科学,2007,29(增刊):411-413.
[20]彭文芳.黄麻纤维的性能研究及服用产品开发[J].陕西纺织, 2007,74:36-39.
[21]彭文芳.黄麻纤维性能及其服用产品开发[J].纺织科技进展,2007(3):83-84.
[22]邵松生.中国黄麻纺织业的发展前景[J].中国纺织经济, 2004(1):45-47.
[23]孙进昌,童华兵.麻类副产物的综合开发与利用价值[J].农产品加工·学刊,2006(12):23-25.
[24]Chen Yiping. Process for enzyme chemically degumming. European patent, Patent number: CN 1232092.
[25] Mussig J,Martens R,Harig H.Hemp Fiber as a Textite Resource[J].Textile Asia,1998,29(5):39~50.
[26] Bruhlmann F,Leapin M,ErismaJm KH,et.al.Enzymatic degumming oframie bast fibers[J]. Journal of biotechnology,2000,76(1):43-50.
[27]祁建民,方平平,徐建堂,等.黄麻红麻生物质高效利用与区域发展产业带建设[J].中国麻业科学,2007,29(2):57-63.
[28]刘瑛,李选才,陈晓蓉,等.麻类作物副产品的综合利用现状[J].江西棉花,2003(1):4-7.
[29]张木祥,郭运玲,邓国华,等.麻类作物副产物综合利用概述[J].中国麻作,1990(4):36-39.
[30]熊和平.弘扬麻业特色优势为新农村建设作贡献[J].中国麻业科学,2007,29(增刊):57-59.
[31]龚友才.恢复黄麻生产开发黄麻新产品[J].中国麻业,2003, 25(6):112-114.
[32]邵群,张惠,边际.功能性油脂——共轭亚油酸研究进展[J].食品科学,2002,23(2):5-7.
[33]张根旺,杨天奎,郭诤.生物活性物质CLA的研究[J].中国油脂,2000,25(6): 13-16.
[34]曹健,黄河勋,张衍荣.帝王菜[J].长江蔬菜,2002(4):12-13.
[35]林建国.印度帝王菜高效栽培技术[J].北京农业,2002(12):8-9.
[36]粟建光,揭雨成,龚友才,等.麻类优异种质的创新与利用研究[J].中国麻业,2004,26(2):56-58.
[37]李宗道著.麻类的理论与技术[M].上海科学技术出版社,1980b:469.
[38]黎宇,程新奇,郭安平.我国黄麻种质资源的研究进展概述[J].中国麻作,1998,20(43):38-41.
[39]粟建光,龚友才,关凤芝,等.麻类种质资源的收集、保存、更新与利用[J].中国麻业,2003,25(1):4-8.
[40]张继成、程新奇、肖瑞芝,等.圆果种黄麻种质资源抗炭疽病鉴定研究[J].作物品种资源,1991(4):34-35.
[41]贾继增.分子标记种质资源鉴定和分子标记育种[J].中国农业科学,1996,29(4):1-10.
[42]陈绵方,吴家琴,薛召东,等.长果种黄麻种质资源对黑点炭疽病抗性的鉴定[J].中国麻作,1990(4):36.
[43]粟建光.我国麻类资源的多样性及其保护利用对策[J].植物遗传资源科学, 2002,3(3):41-46.
[44]郑云雨,王英娇.黄麻育种与高产栽培技术[M].中国农业出版社,北京,1994,12:1-85.
[45]祁建民,刘国忠.黄麻、红麻品种与高效配套技术[M].台湾出版社,2007:31.
[46]龚友才,粟建光.麻类作物诱变育种的现状与进展[J].中国麻业,2002(4):14-17.
[47]林荔辉,祁建民.红麻无刺新型品种金光1号的选育[J].中国麻业,2004,14(3):157-161.
[48]郑志炡.我国黄麻品种资源的研究[J].作物学报,1964(1):36-50.
[49]祁建民,李维民,林荔辉,等.黄麻种质资源数量分类研究[J].作物学报,1996,22(5):587-594.
[50]程新奇,郭安平,肖瑞芝,等.黄麻种质资源的鉴定与利用分析[J].中国麻作,1993(4):1-8.
[51]肖瑞芝.我国黄麻品种资源主要类型与经济性状研究[J].中国麻作,1982(1):30-33
[52]中国农业科学院麻类研究所主编.中国黄红麻品种志[M].中国农业出版社,北京:10-13.
[53]祁建民,刘国忠著.黄麻红麻品种与高效配套技术[M].台湾出版社,2007,36-40.
[54] Banerjee, I. Chromosome mumber of Indian Crop Plants. A. Chromosome numbers in jute[J]. Indian Bot. Soc. 1932a,11:82-85.
[55]李宗道等.麻类生物工程进展[M].中国农业出版社,北京,1999(1):188-189.
[56] Banerjee, I. The development of flower and pollen in jute[J]. Indian J. Agric. Sci. 1933,3:116-121.
[57] Rao, N. S, Datta, R. M.Chromosomes of the genus Corchorus[J]. Nature,1953:131-132.
[58]朱秀英,陈祖耕,林学建,等.黄麻染色体显带的初步研究[J].中国麻作,1986(1):1-2.
[59]朱凤绥,何广文,肖瑞芝,等.麻类作物的染色体组型分析及Giemsa带型初步观察[J].中国麻作,1981:(3)1-9.
[60]肖瑞芝.圆果种黄麻(Corchorus capsularis)品种不同形态特征的细胞学研究[J].中国麻作,1986,3:1-5.
[61]陈祥云,李树川.红麻花粉植株诱导的研究[J].中国麻作,1985(3):1-4,32.
[62] Islan A S,Rahman S M E.Plant regeneration in Jute Newsletter[J].International Plant Biotechnology Network,1986,6:10.
[63] Ahmed G,Hossain A B M,Tslam M S.Regeneration of multiple shoots in jute corchorus olitorius (var.04) from cotyledon and hypocotyle esplants of germinating seeds[J].Indian Journal of Experimental Biology,1989,27(4):16-18.
[64] Ghosh K S,Bandyopadhyay,P D Ghosh.Gallus culture and plantlet regeneration in jute (corchorus capsularis 1) Bangladish[J].BOT,1992,21 (1):119-122.
[65]李宗道,张福泉,郑思乡,等.麻类生物工程进展[M].中国农业出版社,1999:183-184.
[66]林荔辉,祁建民,方平平,等.红麻无刺新型品种金光1号的选肓[J].中国麻业,2004(4):35-38.
[67]陶爱芬,祁建民,李爱青,等.红麻优育种质资源遗传多样性与亲缘关系的ISSR分析[J].作物学报,2005,31(12):1668-1671.
[68]谢小芳,黄代青,吴为人.长果种黄麻单染色体未克隆DNA文库的构建[J] .作物学报, 2006, 32(8):1184- 1187.
[69] GANGULY P,SAMANTA A,NANDI D,et al.Some studies of cross-linking on jute fabric with different preparatory mutes[M].Colourage,1987(7):19-24.
[70]孙庆祥,罗才安,刘正初,等.黄麻和红麻微生物脱胶研究Ⅲ陆地湿润脱胶技术研究[J].中国麻作,1991(2):29-32.
[71]孙庆祥,刘正初,彭源德,等.黄麻和红麻微生物脱胶研究Ⅳ鲜皮和鲜杆陆地湿润脱胶技术研究[J].中国麻作,1992(1):29-32.
[72]李宗道等.麻类生物工程进展[M].中国农业出版社.1999(1):276-277.
[73]胡开辉,李雪仙.红麻脱胶菌的筛选及其产酶条件[J].河南科技大学学报:自然科学版,2006,27(5):79-82.
[74]张双,吴丽莉.黄麻纤维生化联合脱胶研究[J].国际纺织导报,2009(3):14-16.
[75]曹红星,孙程旭,吴翼,等.分子标记在棕榈科植物遗传育种中的应用[J].中国农学通报,2009,25(03):279-282.
[76]殷继艳,张建国.应用于杨树遗传进化研究中常见的几种遗传标记方法[J].安徽农业科学,2007,35(34):65-68.
[77]李桂兰,闫高.分子标记技术的发展及应用[ J].生物学通报,2006,41 (2):17 -19.
[78]贺学勤,刘庆昌,翟红.用RAPD、ISSR和AFLP标记分析系谱关系明确的甘薯品种的亲缘关系[J].作物学报,20,31(10):1300—1304.
[79] Russell J R,Fuller J D,Macaulay M,et al.Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs[J].Theoretical and Applied Genetics,1997,95:714-722.
[80] Pejic I,Ajmone Marsan P,Morgante M.comparative analysis of geneticsimilarity among maize inbred lines detected by RFLPs,RAPDs,SSRs and AFLPs[J].Theoretical and Applied Genetics,1998,97:1248-1255.
[81]高力,等.麻类作物分子标记技术应用进展[J].中国麻业,2005,27(6):322-326.
[82] William S.J.K,Kubelik A.R.L,Ivak K.L.et al.DNA polymorphisms amplified by arbitrary primers are useful as geneticmarkers [J].Nucleic Acids Research,1990,18 (22):6531- 6535.
[83] Welsh J,Mcclelland M. Fingerprinting genomes using PCR with arbitrary primers [J]. Nucleic Acids Research,1990,16:7213- 7218.
[84]周东新,祁建民,吴为人,等.黄麻DNA提取与RAPD反应体系的建立[J].福建农林大学学报,2001,30(1):334-339.
[85]祁建民,周东新,吴为人,等.应用RAPD指纹探讨黄麻属种间遗传多样性及其亲缘关系[J].遗传学报,2003,30(10):936-932.
[86] Mohammad Belayat Hossain , Samiul Haque , Haseena Khan . DNA Fingerprinting of Jute Germplasm by RAPD[J].Journal of Biochemistry and Molecular Biology,2002,35(4):414–419.
[87] Mohammad Belayat Hossain,Aleya Awal,Mohammad Aminur Rahman,Samiul Haque and Haseena Khan.Distinction between Cold-sensitive and -tolerant Jute by DNA Polymorphisms[J].Journal of Biochemistry and Molecular Biology,2003,36(5):427–432.
[88] A Roy,A Bandyopadhyay,AK Mahapatra,SK Ghosh,NK.Evaluation of genetic diversity in jute (Corchorus species) using STMS , ISSR and RAPD markers[J].Plant breeding,2006(5):134-135.
[89] Zabeau M,Vos P.Selective restriction fragment amplification:a general method for DNA fingerprinting[J].European Patent Application (Publication No.05348AI).Paris:European Patent Office,1993(7):26-29.
[90]翁曼丽,谢纬武,伏健民,等.新一代分子标记技术——AFLP应用与环境[J] .生物学报,1996,2(4):424-429.
[91]王斌,翁曼丽.AFLP原理及其应用[J].杂交水稻,1996,5:27-30.
[92]吴敏生,戴景瑞.扩增片段长度多态性(AFLP)——一种新的分子标记技术[J].植物学通报,1998,15(4):68-74.
[93]胡尊红,郭鸿彦,郭孟,等.AFLP技术在大麻遗传研究中的应用[J].中国麻业科学,2009(5):296-300.
[94]李胡董,裴忠有,沈平,等.分子标记技术及其在作物育种研究上的应用[J].沈阳农业大学学报,2001,32(2):150-154.
[95]邵映田,牛永春,朱直惶,等.小麦抗条锈病基因Yrl0的AFLP标记[J].科学通报,2001,46(8):669-672.
[96] HillM,Witsenboer H,ZabeauM,et al.PCR based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactucaspp[J].Theoretical and Applied Genetics,1996,93 ( 8):1202-1210.
[97] Paul S, Wachira F N, PowellW, et al. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Cam elliasinebsis (L.) O. Kuntze) revealed by AFLP markers[J].Theoretical and App lied Genetics,1997,94 (2):255– 263.
[98] Vos P,Hogers Bieeker M,et a1.A new technique for DNA fmgerprinting[J].Acids Res,1995,21:4407-4414.
[99] Zietkiewicze,Rafalskia,Labudad.Genome figner printing by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomic- s,1994,20:176-183.
[100]张立荣,徐大庆,刘大群,等.SSR和ISSR分子标记及其在植物遗传育种研究中的应用[J].河北农业大学学报,2002,25(1):90-94.
[101]祁建民,周东新,吴为人,等.ISSR和RAPD标记检测黄麻属遗传多样性的比较研究[J].中国农业科学报,2004,37(12):2006-2011.
[102]陈晖,祁建民,等.黄红麻生物技术研究进展与展望[J].中国麻业科学,2006,25(5):226-230.
[103] Litt M,Luty JA.A Hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene[J].American Journal Human Genetics,1989,4:397-401.
[104] Tautz D.Hyper variability of simple sequences as a general polymorphic DNA markers[J].Nucl Acids Res,1989,17:6463-6471.
[105]刘卫东.SSR标记分离技术的研究概况[J].咸宁学院学报,2007(6):80-84.
[106] Wu K S and Tanksly S D.Abundance,polymorphism and gene tic mapping of microsattellite inrice[J].Molecular General Genetics,1993,241:225-235.
[107]袁昭岚,沈颂东,黄鹤忠,等.SSR和ISSR分子标记技术有其在遗传多态性方面的应用[J].水产养殖,2005,26(2):10-13.
[108] Morgan M,Olivieri AM.PCR—amplified microsatellite as markers in plant genetic[J].PlantJournal,1993,3:175-182.
[109]Young.N.D.A cautiously optimistic vision or marker-assisted breedin- g[J].Molecular Breeding,1999,5:505-510.
[110] Ayala L,Henry M,Gonzalez—de D,et a1.A diagnostic molecular marker allowing the study of the Iuterruediuru—derived resistance to BYDV in bread wheat segregating populations[J].Theor Appl Genet,2001,102:942-949.
[111] Zhu ZhenDong , Jia JiZeng . Microsatellite Marker Development and Applications in Wheat Genetics and Breading [J].HEREDITAS,2003,25(3):355-360.
[112] Prasad M,Roy J' Roy J K The use of microsatellites for detecting DNA polymorphism,genotype identification and genetic diversity in wheat[J].Theor Appl Genet 2000,100:584-592.
[113]吴晓雷,贺超英,陈受宜.用SSR分子标记研究大豆属种间亲缘进化关系[J].遗传学报,200l,28(4):359-366.
[114] Rongwei J.The use of microsatellite DNA markers for soybean genotype identification[J].Theor Appl Genet,1995,90:43-48.
[115] Bell C J,Ecker J H.Assignment of 30 microsattellite loci to the linkage map of Arabidopsis[J].Genomics,1994,19:137-144.
[116] Saghai Maroof M A,Biyasher R M,Yang G Y,et a1.Extraordinarily polyporphism microsatellite DNA in barley species diversity,chromosomal 1ocation a population dynamics[J].Proceeding of the national academy ofscience of USA,1 994,91:5466-5470.
[117] A. Basu,M. Ghosh,R. Meyer,W. Powell,S. L. Basak,S. K.Sen.Analysis of Genetic Diversity in Cultivated Jute Determined by Means of SSR Markers and AFLP Profiling[J].Crop Science,2004(2):44.
[118] Reyazul R. Mir,Sachin Rustgi,Shailendra Sharma.A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute[J].Gupta Euphytica. 2008,16(1):413–427.
[119] Bostein D,White R L,Skolnick M,et a1.Construction of a genetic linkage map in human using restriction fragment polymorphism[J].Am J Hum Genet,1980,32:314-331.
[120]戴灼华,王亚馥,粟冀玟.遗传学(第二版)[M].高等教育出版社,2008:427.
[121]黄中文,王莹,辛慧,等.RFLP原理及在作物基因定位和育种中的应用[J].河南职技师院学报,2000,28(4):24-28.
[122]吕瑞玲,吴小凤,刘敏超.分子标记技术及在水稻遗传研究中的应用[J].中国农学通报,2009,25(04):65-73.
[123]苏光华,张元跃.SNP分子标记及其应用[J].畜牧兽医科技信息,2005(12):11-13.
[124] Brookes A.The essence of SNPs[J].Gene,1999,23(4):177-186.
[125] Rafalski J . A.Application of single nucleotide polymorphisms in crop genetics[J].Current Opinion in Plant Biology,2002a,5:94 -100.
[126] Zuccarello G C, Burger G, West J A, King R .A mitochondrial marker for red algal intraspecific relationships[J]. Mol Ecol,1999,8:1443-1447.
[127] Batley J B, Hayes P K.Development of high throughout single nucleotide polymorphism genotyping for the analysis of Nodularia (Cyanobacteria) population genetics [J]. Phycol,2003,39:248-252.
[128] Wang D G,Fan J B,Siao C,et a1.Large scale identification,mapping,and genotyping of single·nucleotide polymorphisms in the human genome [J].Science,1998,280:1077—1082.
[129]邹喻苹,葛颂.新一代分子标记SNPs及其应用[J].生物多样性,2003,11(5):370-382.
[130] Hu J ,Vick B A.Target region amplification polymorphism:A novel marker technique for plant genotyping [J].Plant Molecular Biology Reporter,2003,21:289 - 294.
[131]王芳,周延清.TRAP标记技术在植物研究中的应用[J].河南农业科学,2008(6):15-17.
[132]郭长奎,于静,罗淑萍.新型分子标记TRAP应用研究进展[J].生命科学研究,2009,13(4):366-369.
[133] Sonrdille P,Singh S,Cadalen T,et al Microsatellite—based deletion bin systemforthe establishment of genetic physical map relationships in wheat[J].Crop Sci,2004,4:12-25.
[134] Hu Joehoa O E,TmeoM J,Vick B A.Application of the TRAP technique to lettuce notyping[J].Euphytics,2005,244:225-235.
[135] Alwala S , SumanA J , AfroA , et a1.Target region amplification polymorphism(TRAP) for assessing genetic diversity in sugarcane germplasm collections[J].Crop Sci,2006,46:448-455.
[136]苗培明,范玲.TRAP分子标记技术在植物分子遗传育种中的应用[J].新疆农业科学,2007,44(S3):50-52.
[137] Li G,Quiros C F.Sequence—related amplified polymorphism(SRAP) ,A new marker system based on a simple PCR reaction :its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461.
[138]柳李旺,龚义勤,黄浩,等.新型分子标记——SRAP与TRAP及其应用[J].遗传,2004,26(5):777-781.
[139]徐操,赵宝华.SRAP分子标记的研究进展及其应用[J].生命科学仪器,2009(7):24-27.
[140]林忠旭,张献龙,聂以春,等.棉花SRA P遗传连锁图构建[J].中国科学通报,2003,48(15):1676—1679.
[141]陈美霞,张广庆,祁建民,等.红麻SRAP、ISSR遗传连锁图构建的初步研究[J].中国麻业科学,2008,30(3):121-127.
[142] RIAZ A,LI G,QURESH Z.Genetie diversity 0f oilseed Brassiea napus inbred lines based Oil sequence related amplified polymophism(SRAP)and its relation to hybrid performance[J].Plant Breeding,2001,120(5):41l-415.
[143]杨平,刘仙俊,冯宗云,等.利用SRAP标记研究四川高原青稞育成品种的遗传多样性[J].遗传,2008,30(1):115-122.
[144]王从彦,胡颖,郭二辉,等.SRAP分子标记分析芫花遗传多样遗传多样性[J] .河南科学,2008,26 (5):554-556.
[145]阮成江,何祯祥.中国植物遗传连锁图谱构建研究进展[J].西北植物学报,2002,22(6):1526-1536.
[146]肖炳光,徐照丽,陈学军,等.利用DH群体构建烤烟分子标记遗传连锁图[J].中国烟草学报,2006,8(12):35-40.
[147]潘俊松,王刚,等.黄瓜SRAP遗传连锁图谱的构建及始花节位的基因定位[J].自然科学进展,2005,2(15):167-172.
[148]沈金雄,易斌,傅廷栋,等.植物数量性状基因定位研究概述[J].植物学通报,2003,20(3):257-263.
[149]李维明,吴为人,卢浩然.小麦7D染色体数量性状基因定位和效应估计的研究[J].作物学报,2000.22(6):641-645.
[150]杨鑫雷,王志伟,张桂寅,等.棉花分子遗传图谱构建和纤维品质性状QTL分析[J].作物学报,2009,35(12):2159-2166.
[151]王永飞,马三梅.刘翠萍,等.分子标记在植物遗传育种中的应用原理及现状[J].西北农林科技大学学报(自然科学版),2001,29(增刊):106-113.
[152] Song W Y,Wang G L,Chen L L.et a1.A receptor kinase—like protein encoded hy the rice disease resistance gene Xa21[J].Science,1995,270:1804-1806.
[153]Yoshimura S,Yamanouchi U,Katayose Y,et a1.Expression of Xa 1.a bacterial blightresistance gene in rice is induced by bacterial inoculation[J].Proe Natl Acad Sci USA,1998,95(4):1663-1668.
[154]张德水,陈受宜.DNA分子标记、基因作图及其在植物遗传育种上的应用[J].生物技术通报,1998,(5):15-22.
[155]余隆其,孙家曾,何广文.黄麻——宽叶长果.作物杂志,1986(1):39.
[156]徐鲜均.黄麻属野生种与栽培种遗传资源多样性研究.福建农林大学硕士学位论文.2009:64.
[157]徐建堂,祁建民,方平平,等.CTAB法提取红麻总DNA技术优化与ISSR和SRAP扩增效果[J].中国麻业科学,2007,29(4):179-183.
[158]单晓政,侯喜林,李英,等.不结球白菜SRAP体系优化与品种聚类分析[J].江苏农业学报,2009,25(3):610-615.
[159]张广庆.应用SRAP和ISSR分子标记构建红麻的遗传连锁图谱.福建农林大学硕士学位论文.2007:28-31.
[160]于拴仓,王永健,郑晓鹰.大白菜分子遗传图谱的构建与分析[J].中国农业科学,2003,36(2):190-195.
[161]吴晓雷,贺超英,王永军,等.大豆遗传图谱的构建和分析[J].遗传学报,2001,28(11):1951-1961
[162] Knox M R , Ellis T H N. Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations[J].Genetics ,2002 ,162 (2) : 861-873.
[163] Kinshita T. Report of the committee on gene symbolization ,nomenclature and linkage group[J] . Rice Genet Newl ,1991 ,8 (1) :2-37.
[164] Kinshita T. Report of the committee on gene symbolization ,nomenclature and linkage group[J] . Rice Genet Newl , 1993 , 10(1) : 7-39.
[165] Coe E H,Polacco M. Gene list and working maps[J]. Maize GenetCoop Newslett ,1995 ,694 (1) : 157-191.
[2]中国农业科学院麻类研究所.中国黄麻红麻品种志[M].农业出版社,1985:10.
[3]张泉.关于植物形态学的思考[J].自然杂志,2002(3):180-183.
[4]方宣钧,吴为人,唐纪良.作物DNA标记辅助育种[M].科学出版社,2000:2.
[5]方嘉禾,常汝镇著.中国作物及其野生近缘植物——经济作物卷[M].中国农业出版社,2007:305-307.
[6]祁建民,李维明,吴为人.黄麻的起源与演化[J].作物学报,1997,23(6):67-68.
[7]胡立勇,丁艳锋主编.作物栽培学[M].高等教育出版社,北京,2009:12.
[8]熊和平主编.麻类作物育种学[M].中国农业科学技术出版社,2008(2):156.
[9]中国农业科学院麻类研究所主编.中国麻类作物栽培学[M].农业出版社,1982:123.
[10]Kundu B C, Jute Worlds Formost, Bast Fibers, et.al. Diseases and pests[J].Economic Botany,1956a, 10(2):103-133.
[11] R.L.史密斯, 1977,李建东等译, 1988.生物学原理和野外生物学[M].科学出版社,北京,14-20.
[12]张金泉,植物地理学[M].重庆出版社,上海, 1989, 224-228.
[13] H.N.瓦维洛夫,1935,许运天译,栽培植物的起源中心[M].农业出版社,北京,1982:31.
[14] Kundu B.C. The Active Principles of corchorus olitorius L(Jute) Leaves[J]. Sciences and Culture, 1951:16.
[15] De Condolle, 1882,俞德浚、蔡希陶译, 1940,农艺植物考源[M].上海:商务印书馆,147-150.
[16] Jennifer M Edmonds.Herbarium survey of Cofchorus L. species[J]. Department of Plant Sciences, University of Oxford,1-2.
[17]李宗道.麻作的理论与技术[M].上海科技出版社,1980:388-390.
[18]卢浩然.中国农百科全书——农作物卷,麻类分支[M].农业出版社,北京,1991:256.
[19]李德芳.红/黄麻新用途综合开发与国际合作[J].中国麻业科学,2007,29(增刊):411-413.
[20]彭文芳.黄麻纤维的性能研究及服用产品开发[J].陕西纺织, 2007,74:36-39.
[21]彭文芳.黄麻纤维性能及其服用产品开发[J].纺织科技进展,2007(3):83-84.
[22]邵松生.中国黄麻纺织业的发展前景[J].中国纺织经济, 2004(1):45-47.
[23]孙进昌,童华兵.麻类副产物的综合开发与利用价值[J].农产品加工·学刊,2006(12):23-25.
[24]Chen Yiping. Process for enzyme chemically degumming. European patent, Patent number: CN 1232092.
[25] Mussig J,Martens R,Harig H.Hemp Fiber as a Textite Resource[J].Textile Asia,1998,29(5):39~50.
[26] Bruhlmann F,Leapin M,ErismaJm KH,et.al.Enzymatic degumming oframie bast fibers[J]. Journal of biotechnology,2000,76(1):43-50.
[27]祁建民,方平平,徐建堂,等.黄麻红麻生物质高效利用与区域发展产业带建设[J].中国麻业科学,2007,29(2):57-63.
[28]刘瑛,李选才,陈晓蓉,等.麻类作物副产品的综合利用现状[J].江西棉花,2003(1):4-7.
[29]张木祥,郭运玲,邓国华,等.麻类作物副产物综合利用概述[J].中国麻作,1990(4):36-39.
[30]熊和平.弘扬麻业特色优势为新农村建设作贡献[J].中国麻业科学,2007,29(增刊):57-59.
[31]龚友才.恢复黄麻生产开发黄麻新产品[J].中国麻业,2003, 25(6):112-114.
[32]邵群,张惠,边际.功能性油脂——共轭亚油酸研究进展[J].食品科学,2002,23(2):5-7.
[33]张根旺,杨天奎,郭诤.生物活性物质CLA的研究[J].中国油脂,2000,25(6): 13-16.
[34]曹健,黄河勋,张衍荣.帝王菜[J].长江蔬菜,2002(4):12-13.
[35]林建国.印度帝王菜高效栽培技术[J].北京农业,2002(12):8-9.
[36]粟建光,揭雨成,龚友才,等.麻类优异种质的创新与利用研究[J].中国麻业,2004,26(2):56-58.
[37]李宗道著.麻类的理论与技术[M].上海科学技术出版社,1980b:469.
[38]黎宇,程新奇,郭安平.我国黄麻种质资源的研究进展概述[J].中国麻作,1998,20(43):38-41.
[39]粟建光,龚友才,关凤芝,等.麻类种质资源的收集、保存、更新与利用[J].中国麻业,2003,25(1):4-8.
[40]张继成、程新奇、肖瑞芝,等.圆果种黄麻种质资源抗炭疽病鉴定研究[J].作物品种资源,1991(4):34-35.
[41]贾继增.分子标记种质资源鉴定和分子标记育种[J].中国农业科学,1996,29(4):1-10.
[42]陈绵方,吴家琴,薛召东,等.长果种黄麻种质资源对黑点炭疽病抗性的鉴定[J].中国麻作,1990(4):36.
[43]粟建光.我国麻类资源的多样性及其保护利用对策[J].植物遗传资源科学, 2002,3(3):41-46.
[44]郑云雨,王英娇.黄麻育种与高产栽培技术[M].中国农业出版社,北京,1994,12:1-85.
[45]祁建民,刘国忠.黄麻、红麻品种与高效配套技术[M].台湾出版社,2007:31.
[46]龚友才,粟建光.麻类作物诱变育种的现状与进展[J].中国麻业,2002(4):14-17.
[47]林荔辉,祁建民.红麻无刺新型品种金光1号的选育[J].中国麻业,2004,14(3):157-161.
[48]郑志炡.我国黄麻品种资源的研究[J].作物学报,1964(1):36-50.
[49]祁建民,李维民,林荔辉,等.黄麻种质资源数量分类研究[J].作物学报,1996,22(5):587-594.
[50]程新奇,郭安平,肖瑞芝,等.黄麻种质资源的鉴定与利用分析[J].中国麻作,1993(4):1-8.
[51]肖瑞芝.我国黄麻品种资源主要类型与经济性状研究[J].中国麻作,1982(1):30-33
[52]中国农业科学院麻类研究所主编.中国黄红麻品种志[M].中国农业出版社,北京:10-13.
[53]祁建民,刘国忠著.黄麻红麻品种与高效配套技术[M].台湾出版社,2007,36-40.
[54] Banerjee, I. Chromosome mumber of Indian Crop Plants. A. Chromosome numbers in jute[J]. Indian Bot. Soc. 1932a,11:82-85.
[55]李宗道等.麻类生物工程进展[M].中国农业出版社,北京,1999(1):188-189.
[56] Banerjee, I. The development of flower and pollen in jute[J]. Indian J. Agric. Sci. 1933,3:116-121.
[57] Rao, N. S, Datta, R. M.Chromosomes of the genus Corchorus[J]. Nature,1953:131-132.
[58]朱秀英,陈祖耕,林学建,等.黄麻染色体显带的初步研究[J].中国麻作,1986(1):1-2.
[59]朱凤绥,何广文,肖瑞芝,等.麻类作物的染色体组型分析及Giemsa带型初步观察[J].中国麻作,1981:(3)1-9.
[60]肖瑞芝.圆果种黄麻(Corchorus capsularis)品种不同形态特征的细胞学研究[J].中国麻作,1986,3:1-5.
[61]陈祥云,李树川.红麻花粉植株诱导的研究[J].中国麻作,1985(3):1-4,32.
[62] Islan A S,Rahman S M E.Plant regeneration in Jute Newsletter[J].International Plant Biotechnology Network,1986,6:10.
[63] Ahmed G,Hossain A B M,Tslam M S.Regeneration of multiple shoots in jute corchorus olitorius (var.04) from cotyledon and hypocotyle esplants of germinating seeds[J].Indian Journal of Experimental Biology,1989,27(4):16-18.
[64] Ghosh K S,Bandyopadhyay,P D Ghosh.Gallus culture and plantlet regeneration in jute (corchorus capsularis 1) Bangladish[J].BOT,1992,21 (1):119-122.
[65]李宗道,张福泉,郑思乡,等.麻类生物工程进展[M].中国农业出版社,1999:183-184.
[66]林荔辉,祁建民,方平平,等.红麻无刺新型品种金光1号的选肓[J].中国麻业,2004(4):35-38.
[67]陶爱芬,祁建民,李爱青,等.红麻优育种质资源遗传多样性与亲缘关系的ISSR分析[J].作物学报,2005,31(12):1668-1671.
[68]谢小芳,黄代青,吴为人.长果种黄麻单染色体未克隆DNA文库的构建[J] .作物学报, 2006, 32(8):1184- 1187.
[69] GANGULY P,SAMANTA A,NANDI D,et al.Some studies of cross-linking on jute fabric with different preparatory mutes[M].Colourage,1987(7):19-24.
[70]孙庆祥,罗才安,刘正初,等.黄麻和红麻微生物脱胶研究Ⅲ陆地湿润脱胶技术研究[J].中国麻作,1991(2):29-32.
[71]孙庆祥,刘正初,彭源德,等.黄麻和红麻微生物脱胶研究Ⅳ鲜皮和鲜杆陆地湿润脱胶技术研究[J].中国麻作,1992(1):29-32.
[72]李宗道等.麻类生物工程进展[M].中国农业出版社.1999(1):276-277.
[73]胡开辉,李雪仙.红麻脱胶菌的筛选及其产酶条件[J].河南科技大学学报:自然科学版,2006,27(5):79-82.
[74]张双,吴丽莉.黄麻纤维生化联合脱胶研究[J].国际纺织导报,2009(3):14-16.
[75]曹红星,孙程旭,吴翼,等.分子标记在棕榈科植物遗传育种中的应用[J].中国农学通报,2009,25(03):279-282.
[76]殷继艳,张建国.应用于杨树遗传进化研究中常见的几种遗传标记方法[J].安徽农业科学,2007,35(34):65-68.
[77]李桂兰,闫高.分子标记技术的发展及应用[ J].生物学通报,2006,41 (2):17 -19.
[78]贺学勤,刘庆昌,翟红.用RAPD、ISSR和AFLP标记分析系谱关系明确的甘薯品种的亲缘关系[J].作物学报,20,31(10):1300—1304.
[79] Russell J R,Fuller J D,Macaulay M,et al.Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs[J].Theoretical and Applied Genetics,1997,95:714-722.
[80] Pejic I,Ajmone Marsan P,Morgante M.comparative analysis of geneticsimilarity among maize inbred lines detected by RFLPs,RAPDs,SSRs and AFLPs[J].Theoretical and Applied Genetics,1998,97:1248-1255.
[81]高力,等.麻类作物分子标记技术应用进展[J].中国麻业,2005,27(6):322-326.
[82] William S.J.K,Kubelik A.R.L,Ivak K.L.et al.DNA polymorphisms amplified by arbitrary primers are useful as geneticmarkers [J].Nucleic Acids Research,1990,18 (22):6531- 6535.
[83] Welsh J,Mcclelland M. Fingerprinting genomes using PCR with arbitrary primers [J]. Nucleic Acids Research,1990,16:7213- 7218.
[84]周东新,祁建民,吴为人,等.黄麻DNA提取与RAPD反应体系的建立[J].福建农林大学学报,2001,30(1):334-339.
[85]祁建民,周东新,吴为人,等.应用RAPD指纹探讨黄麻属种间遗传多样性及其亲缘关系[J].遗传学报,2003,30(10):936-932.
[86] Mohammad Belayat Hossain , Samiul Haque , Haseena Khan . DNA Fingerprinting of Jute Germplasm by RAPD[J].Journal of Biochemistry and Molecular Biology,2002,35(4):414–419.
[87] Mohammad Belayat Hossain,Aleya Awal,Mohammad Aminur Rahman,Samiul Haque and Haseena Khan.Distinction between Cold-sensitive and -tolerant Jute by DNA Polymorphisms[J].Journal of Biochemistry and Molecular Biology,2003,36(5):427–432.
[88] A Roy,A Bandyopadhyay,AK Mahapatra,SK Ghosh,NK.Evaluation of genetic diversity in jute (Corchorus species) using STMS , ISSR and RAPD markers[J].Plant breeding,2006(5):134-135.
[89] Zabeau M,Vos P.Selective restriction fragment amplification:a general method for DNA fingerprinting[J].European Patent Application (Publication No.05348AI).Paris:European Patent Office,1993(7):26-29.
[90]翁曼丽,谢纬武,伏健民,等.新一代分子标记技术——AFLP应用与环境[J] .生物学报,1996,2(4):424-429.
[91]王斌,翁曼丽.AFLP原理及其应用[J].杂交水稻,1996,5:27-30.
[92]吴敏生,戴景瑞.扩增片段长度多态性(AFLP)——一种新的分子标记技术[J].植物学通报,1998,15(4):68-74.
[93]胡尊红,郭鸿彦,郭孟,等.AFLP技术在大麻遗传研究中的应用[J].中国麻业科学,2009(5):296-300.
[94]李胡董,裴忠有,沈平,等.分子标记技术及其在作物育种研究上的应用[J].沈阳农业大学学报,2001,32(2):150-154.
[95]邵映田,牛永春,朱直惶,等.小麦抗条锈病基因Yrl0的AFLP标记[J].科学通报,2001,46(8):669-672.
[96] HillM,Witsenboer H,ZabeauM,et al.PCR based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactucaspp[J].Theoretical and Applied Genetics,1996,93 ( 8):1202-1210.
[97] Paul S, Wachira F N, PowellW, et al. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Cam elliasinebsis (L.) O. Kuntze) revealed by AFLP markers[J].Theoretical and App lied Genetics,1997,94 (2):255– 263.
[98] Vos P,Hogers Bieeker M,et a1.A new technique for DNA fmgerprinting[J].Acids Res,1995,21:4407-4414.
[99] Zietkiewicze,Rafalskia,Labudad.Genome figner printing by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomic- s,1994,20:176-183.
[100]张立荣,徐大庆,刘大群,等.SSR和ISSR分子标记及其在植物遗传育种研究中的应用[J].河北农业大学学报,2002,25(1):90-94.
[101]祁建民,周东新,吴为人,等.ISSR和RAPD标记检测黄麻属遗传多样性的比较研究[J].中国农业科学报,2004,37(12):2006-2011.
[102]陈晖,祁建民,等.黄红麻生物技术研究进展与展望[J].中国麻业科学,2006,25(5):226-230.
[103] Litt M,Luty JA.A Hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene[J].American Journal Human Genetics,1989,4:397-401.
[104] Tautz D.Hyper variability of simple sequences as a general polymorphic DNA markers[J].Nucl Acids Res,1989,17:6463-6471.
[105]刘卫东.SSR标记分离技术的研究概况[J].咸宁学院学报,2007(6):80-84.
[106] Wu K S and Tanksly S D.Abundance,polymorphism and gene tic mapping of microsattellite inrice[J].Molecular General Genetics,1993,241:225-235.
[107]袁昭岚,沈颂东,黄鹤忠,等.SSR和ISSR分子标记技术有其在遗传多态性方面的应用[J].水产养殖,2005,26(2):10-13.
[108] Morgan M,Olivieri AM.PCR—amplified microsatellite as markers in plant genetic[J].PlantJournal,1993,3:175-182.
[109]Young.N.D.A cautiously optimistic vision or marker-assisted breedin- g[J].Molecular Breeding,1999,5:505-510.
[110] Ayala L,Henry M,Gonzalez—de D,et a1.A diagnostic molecular marker allowing the study of the Iuterruediuru—derived resistance to BYDV in bread wheat segregating populations[J].Theor Appl Genet,2001,102:942-949.
[111] Zhu ZhenDong , Jia JiZeng . Microsatellite Marker Development and Applications in Wheat Genetics and Breading [J].HEREDITAS,2003,25(3):355-360.
[112] Prasad M,Roy J' Roy J K The use of microsatellites for detecting DNA polymorphism,genotype identification and genetic diversity in wheat[J].Theor Appl Genet 2000,100:584-592.
[113]吴晓雷,贺超英,陈受宜.用SSR分子标记研究大豆属种间亲缘进化关系[J].遗传学报,200l,28(4):359-366.
[114] Rongwei J.The use of microsatellite DNA markers for soybean genotype identification[J].Theor Appl Genet,1995,90:43-48.
[115] Bell C J,Ecker J H.Assignment of 30 microsattellite loci to the linkage map of Arabidopsis[J].Genomics,1994,19:137-144.
[116] Saghai Maroof M A,Biyasher R M,Yang G Y,et a1.Extraordinarily polyporphism microsatellite DNA in barley species diversity,chromosomal 1ocation a population dynamics[J].Proceeding of the national academy ofscience of USA,1 994,91:5466-5470.
[117] A. Basu,M. Ghosh,R. Meyer,W. Powell,S. L. Basak,S. K.Sen.Analysis of Genetic Diversity in Cultivated Jute Determined by Means of SSR Markers and AFLP Profiling[J].Crop Science,2004(2):44.
[118] Reyazul R. Mir,Sachin Rustgi,Shailendra Sharma.A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute[J].Gupta Euphytica. 2008,16(1):413–427.
[119] Bostein D,White R L,Skolnick M,et a1.Construction of a genetic linkage map in human using restriction fragment polymorphism[J].Am J Hum Genet,1980,32:314-331.
[120]戴灼华,王亚馥,粟冀玟.遗传学(第二版)[M].高等教育出版社,2008:427.
[121]黄中文,王莹,辛慧,等.RFLP原理及在作物基因定位和育种中的应用[J].河南职技师院学报,2000,28(4):24-28.
[122]吕瑞玲,吴小凤,刘敏超.分子标记技术及在水稻遗传研究中的应用[J].中国农学通报,2009,25(04):65-73.
[123]苏光华,张元跃.SNP分子标记及其应用[J].畜牧兽医科技信息,2005(12):11-13.
[124] Brookes A.The essence of SNPs[J].Gene,1999,23(4):177-186.
[125] Rafalski J . A.Application of single nucleotide polymorphisms in crop genetics[J].Current Opinion in Plant Biology,2002a,5:94 -100.
[126] Zuccarello G C, Burger G, West J A, King R .A mitochondrial marker for red algal intraspecific relationships[J]. Mol Ecol,1999,8:1443-1447.
[127] Batley J B, Hayes P K.Development of high throughout single nucleotide polymorphism genotyping for the analysis of Nodularia (Cyanobacteria) population genetics [J]. Phycol,2003,39:248-252.
[128] Wang D G,Fan J B,Siao C,et a1.Large scale identification,mapping,and genotyping of single·nucleotide polymorphisms in the human genome [J].Science,1998,280:1077—1082.
[129]邹喻苹,葛颂.新一代分子标记SNPs及其应用[J].生物多样性,2003,11(5):370-382.
[130] Hu J ,Vick B A.Target region amplification polymorphism:A novel marker technique for plant genotyping [J].Plant Molecular Biology Reporter,2003,21:289 - 294.
[131]王芳,周延清.TRAP标记技术在植物研究中的应用[J].河南农业科学,2008(6):15-17.
[132]郭长奎,于静,罗淑萍.新型分子标记TRAP应用研究进展[J].生命科学研究,2009,13(4):366-369.
[133] Sonrdille P,Singh S,Cadalen T,et al Microsatellite—based deletion bin systemforthe establishment of genetic physical map relationships in wheat[J].Crop Sci,2004,4:12-25.
[134] Hu Joehoa O E,TmeoM J,Vick B A.Application of the TRAP technique to lettuce notyping[J].Euphytics,2005,244:225-235.
[135] Alwala S , SumanA J , AfroA , et a1.Target region amplification polymorphism(TRAP) for assessing genetic diversity in sugarcane germplasm collections[J].Crop Sci,2006,46:448-455.
[136]苗培明,范玲.TRAP分子标记技术在植物分子遗传育种中的应用[J].新疆农业科学,2007,44(S3):50-52.
[137] Li G,Quiros C F.Sequence—related amplified polymorphism(SRAP) ,A new marker system based on a simple PCR reaction :its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461.
[138]柳李旺,龚义勤,黄浩,等.新型分子标记——SRAP与TRAP及其应用[J].遗传,2004,26(5):777-781.
[139]徐操,赵宝华.SRAP分子标记的研究进展及其应用[J].生命科学仪器,2009(7):24-27.
[140]林忠旭,张献龙,聂以春,等.棉花SRA P遗传连锁图构建[J].中国科学通报,2003,48(15):1676—1679.
[141]陈美霞,张广庆,祁建民,等.红麻SRAP、ISSR遗传连锁图构建的初步研究[J].中国麻业科学,2008,30(3):121-127.
[142] RIAZ A,LI G,QURESH Z.Genetie diversity 0f oilseed Brassiea napus inbred lines based Oil sequence related amplified polymophism(SRAP)and its relation to hybrid performance[J].Plant Breeding,2001,120(5):41l-415.
[143]杨平,刘仙俊,冯宗云,等.利用SRAP标记研究四川高原青稞育成品种的遗传多样性[J].遗传,2008,30(1):115-122.
[144]王从彦,胡颖,郭二辉,等.SRAP分子标记分析芫花遗传多样遗传多样性[J] .河南科学,2008,26 (5):554-556.
[145]阮成江,何祯祥.中国植物遗传连锁图谱构建研究进展[J].西北植物学报,2002,22(6):1526-1536.
[146]肖炳光,徐照丽,陈学军,等.利用DH群体构建烤烟分子标记遗传连锁图[J].中国烟草学报,2006,8(12):35-40.
[147]潘俊松,王刚,等.黄瓜SRAP遗传连锁图谱的构建及始花节位的基因定位[J].自然科学进展,2005,2(15):167-172.
[148]沈金雄,易斌,傅廷栋,等.植物数量性状基因定位研究概述[J].植物学通报,2003,20(3):257-263.
[149]李维明,吴为人,卢浩然.小麦7D染色体数量性状基因定位和效应估计的研究[J].作物学报,2000.22(6):641-645.
[150]杨鑫雷,王志伟,张桂寅,等.棉花分子遗传图谱构建和纤维品质性状QTL分析[J].作物学报,2009,35(12):2159-2166.
[151]王永飞,马三梅.刘翠萍,等.分子标记在植物遗传育种中的应用原理及现状[J].西北农林科技大学学报(自然科学版),2001,29(增刊):106-113.
[152] Song W Y,Wang G L,Chen L L.et a1.A receptor kinase—like protein encoded hy the rice disease resistance gene Xa21[J].Science,1995,270:1804-1806.
[153]Yoshimura S,Yamanouchi U,Katayose Y,et a1.Expression of Xa 1.a bacterial blightresistance gene in rice is induced by bacterial inoculation[J].Proe Natl Acad Sci USA,1998,95(4):1663-1668.
[154]张德水,陈受宜.DNA分子标记、基因作图及其在植物遗传育种上的应用[J].生物技术通报,1998,(5):15-22.
[155]余隆其,孙家曾,何广文.黄麻——宽叶长果.作物杂志,1986(1):39.
[156]徐鲜均.黄麻属野生种与栽培种遗传资源多样性研究.福建农林大学硕士学位论文.2009:64.
[157]徐建堂,祁建民,方平平,等.CTAB法提取红麻总DNA技术优化与ISSR和SRAP扩增效果[J].中国麻业科学,2007,29(4):179-183.
[158]单晓政,侯喜林,李英,等.不结球白菜SRAP体系优化与品种聚类分析[J].江苏农业学报,2009,25(3):610-615.
[159]张广庆.应用SRAP和ISSR分子标记构建红麻的遗传连锁图谱.福建农林大学硕士学位论文.2007:28-31.
[160]于拴仓,王永健,郑晓鹰.大白菜分子遗传图谱的构建与分析[J].中国农业科学,2003,36(2):190-195.
[161]吴晓雷,贺超英,王永军,等.大豆遗传图谱的构建和分析[J].遗传学报,2001,28(11):1951-1961
[162] Knox M R , Ellis T H N. Excess heterozygosity contributes to genetic map expansion in pea recombinant inbred populations[J].Genetics ,2002 ,162 (2) : 861-873.
[163] Kinshita T. Report of the committee on gene symbolization ,nomenclature and linkage group[J] . Rice Genet Newl ,1991 ,8 (1) :2-37.
[164] Kinshita T. Report of the committee on gene symbolization ,nomenclature and linkage group[J] . Rice Genet Newl , 1993 , 10(1) : 7-39.
[165] Coe E H,Polacco M. Gene list and working maps[J]. Maize GenetCoop Newslett ,1995 ,694 (1) : 157-191.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |