盐胁迫对毛竹实生苗叶片电阻抗参数及叶绿素荧光特性的影响
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摘要
毛竹(Phyllostachys edulis)为禾本科(Gramineae)竹亚科(Bambasoideae)刚竹属多年生植物,是我国重要的经济竹种之一,不仅具有很高的经济价值,而且具有良好的生态功能。为了选育适应性更广的毛竹品系,本试验进行了NaCl对毛竹生理生化影响的研究,以其为进一步选育提供理论参考。研究目的是验证电阻抗图谱参数与耐盐性及生理指标的相关性,分析NaCl胁迫下毛竹叶片的叶绿素荧光特性的参数变化,了解NaCl胁迫对毛竹叶片光合机制的影响。研究了在不同NaCl浓度(0.00 %,0.15 %,0.25 %,0.35 %,0.45 %,0.55 %,0.65 %)梯度胁迫下毛竹叶片的电阻抗图谱参数、叶片膜透性、游离脯氨酸、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、丙二醛(MDA)含量和可溶性蛋白质含量的生理指标的变化以及NaCl胁迫下对毛竹叶片的叶绿素荧光特性的影响。
主要结果如下:
1.随着NaCl浓度的增加和长时间处理,毛竹叶片的电阻抗图谱和EIS参数发生变化。
2. NaCl胁迫下毛竹叶片的膜透性的变化与胞外电阻、弛豫时间之间呈显著的负相关(决定系数分别为R~2=0.75、R~2=0.76)。
3. NaCl胁迫下毛竹叶片的脯氨酸含量和SOD活性与胞内电阻、胞外电阻和弛豫时间之间呈极显著负相关(决定系数分别为R~2=0.66、R~2=0.95、R~2=0.88和R~2=0.69、R~2=0.70、R~2=0.80)。
4. NaCl胁迫下毛竹叶片的POD的活性与胞外电阻和弛豫时间呈显著的正相关,与弛豫时间分布系数呈负相关(决定系数分别为R~2=0.70、R~2=0.76、R~2=0.51)。
5. NaCl胁迫下毛竹叶片中丙二醛的含量与胞外电阻,胞内电阻和弛豫时间呈显著的正相关(决定系数分别为R~2=0. 89、R~2=0.55、R~2=0.59)。
6. NaCl胁迫下毛竹叶片中可溶性蛋白含量与弛豫时间分布系数呈显著的正相关。
7.随着NaCl浓度的增加和长时间胁迫,毛竹叶片的叶绿素荧光参数ΦPSⅡ、ETR、Fv/Fm、qP均呈整体下降,qN呈整体先上升后下降。
8.高NaCl浓度(浓度≧0.35%)胁迫,抑制毛竹叶片的光合作用和毛竹叶片光化学反应启动速率,并降低毛竹叶片光能利用率。
Moso bamboo (Phyllostachys edulis), a perennial plant, belonging to Phyllostachys in Gramineae Bambusoideae, is one of the important economic species of bamboo. They also have high economic benefit, as well as social benefit and ecological benefit. In this paper, the effects of NaCl stress on parameters of electrical impedance spectroscopy (EIS), the physiological indicators and the chlorophyll fluorescence characteristics of Moso bamboo seedling leaves were investigated. The aim of the study was to verify the relation between the parameters of EIS and salt tolerance, and between parameters of EIS and those of physiological indicators, and analyse the changes of chlorophyll fluorescence parameters of Moso bamboo seedling leaves under NaCl stress and understand the effects of NaCl stress on the photosynthetic mechanism of Moso bamboo seedling leaves.
The main results are as follows:
1. With increase of NaCl concentration and long time stress, change of electrical impedance spectroscopy and EIS parameters of Moso bamboo seedling leaves occurred.
2. There were significant negative correlations between extracellular resistance, relaxation time and change of membrane permeability of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0.75 and R~2=0.76, respectively).
3. There were significant negative correlations among EIS parameters extracellular resistance, intracellular resistance, relaxation time and physiological indicators proline content as well as SOD activity of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0.66, R~2=0.95, R~2=0.88 and R~2=0.69, R~2=0.70 and R~2=0.80, respectively).
4. There were significant positive correlations among extracellular resistance as well as relaxation time and POD activity of Moso bamboo seedling leaves, while the negative correlation between distribution coefficient of relaxation time and POD activity of Moso bamboo seedling leaves was found under NaCl stress (The coefficient of determination was R~2=0.70, R~2=0.76 and R~2=0.51, respectively).
5. There were significant positive correlations among extracellular resistance, intracellular resistance, relaxation time and MDA content of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0. 89, R~2=0.55 and R~2=0.59, respectively).
6. There were significant positive correlations between distribution coefficient of relaxation time and content of soluble protein under NaCl stress.
7. With increase of NaCl concentration and long time stress, the chlorophyll fluorescence PARameterΦPSⅡ, ETR, Fv/Fm, and qP decreased, whereas qN increased firstly, and then decreased.
8. High NaCl concentration (≧0.35%) stress inhibited photosynthesis and starting rate of photochemical reaction, and reduced light utilization efficiency of Moso bamboo seedling leaves.
主要结果如下:
1.随着NaCl浓度的增加和长时间处理,毛竹叶片的电阻抗图谱和EIS参数发生变化。
2. NaCl胁迫下毛竹叶片的膜透性的变化与胞外电阻、弛豫时间之间呈显著的负相关(决定系数分别为R~2=0.75、R~2=0.76)。
3. NaCl胁迫下毛竹叶片的脯氨酸含量和SOD活性与胞内电阻、胞外电阻和弛豫时间之间呈极显著负相关(决定系数分别为R~2=0.66、R~2=0.95、R~2=0.88和R~2=0.69、R~2=0.70、R~2=0.80)。
4. NaCl胁迫下毛竹叶片的POD的活性与胞外电阻和弛豫时间呈显著的正相关,与弛豫时间分布系数呈负相关(决定系数分别为R~2=0.70、R~2=0.76、R~2=0.51)。
5. NaCl胁迫下毛竹叶片中丙二醛的含量与胞外电阻,胞内电阻和弛豫时间呈显著的正相关(决定系数分别为R~2=0. 89、R~2=0.55、R~2=0.59)。
6. NaCl胁迫下毛竹叶片中可溶性蛋白含量与弛豫时间分布系数呈显著的正相关。
7.随着NaCl浓度的增加和长时间胁迫,毛竹叶片的叶绿素荧光参数ΦPSⅡ、ETR、Fv/Fm、qP均呈整体下降,qN呈整体先上升后下降。
8.高NaCl浓度(浓度≧0.35%)胁迫,抑制毛竹叶片的光合作用和毛竹叶片光化学反应启动速率,并降低毛竹叶片光能利用率。
Moso bamboo (Phyllostachys edulis), a perennial plant, belonging to Phyllostachys in Gramineae Bambusoideae, is one of the important economic species of bamboo. They also have high economic benefit, as well as social benefit and ecological benefit. In this paper, the effects of NaCl stress on parameters of electrical impedance spectroscopy (EIS), the physiological indicators and the chlorophyll fluorescence characteristics of Moso bamboo seedling leaves were investigated. The aim of the study was to verify the relation between the parameters of EIS and salt tolerance, and between parameters of EIS and those of physiological indicators, and analyse the changes of chlorophyll fluorescence parameters of Moso bamboo seedling leaves under NaCl stress and understand the effects of NaCl stress on the photosynthetic mechanism of Moso bamboo seedling leaves.
The main results are as follows:
1. With increase of NaCl concentration and long time stress, change of electrical impedance spectroscopy and EIS parameters of Moso bamboo seedling leaves occurred.
2. There were significant negative correlations between extracellular resistance, relaxation time and change of membrane permeability of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0.75 and R~2=0.76, respectively).
3. There were significant negative correlations among EIS parameters extracellular resistance, intracellular resistance, relaxation time and physiological indicators proline content as well as SOD activity of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0.66, R~2=0.95, R~2=0.88 and R~2=0.69, R~2=0.70 and R~2=0.80, respectively).
4. There were significant positive correlations among extracellular resistance as well as relaxation time and POD activity of Moso bamboo seedling leaves, while the negative correlation between distribution coefficient of relaxation time and POD activity of Moso bamboo seedling leaves was found under NaCl stress (The coefficient of determination was R~2=0.70, R~2=0.76 and R~2=0.51, respectively).
5. There were significant positive correlations among extracellular resistance, intracellular resistance, relaxation time and MDA content of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0. 89, R~2=0.55 and R~2=0.59, respectively).
6. There were significant positive correlations between distribution coefficient of relaxation time and content of soluble protein under NaCl stress.
7. With increase of NaCl concentration and long time stress, the chlorophyll fluorescence PARameterΦPSⅡ, ETR, Fv/Fm, and qP decreased, whereas qN increased firstly, and then decreased.
8. High NaCl concentration (≧0.35%) stress inhibited photosynthesis and starting rate of photochemical reaction, and reduced light utilization efficiency of Moso bamboo seedling leaves.
引文
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