夏枯草种子质量标准及种子活力初步研究
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摘要
夏枯草(Prunella vulgaris L.)为唇形科夏枯草属多年生草本植物,以干燥果穗入药,临床应用广泛,同时还可用于化工、食品以及观赏等。
优良的种质是确保中药材质量的关键,研究药用植物种质特性、种子活力具有十分重要的意义。本文主要研究了夏枯草种子质量标准、提高夏枯草种子活力的引发技术,以及夏枯草种子粘膜的基本特性。研究结果如下:
1.通过对34个居群夏枯草种子进行净度、千粒重、含水量、相对电导率以及发芽检测,各项指标测定结果再进行K聚类分析,结果表明:不同居群夏枯草种子净度、千粒重、含水量、相对电导率、发芽结果差异极显著;K聚类得到各项指标的3个中心值;根据各个指标的中心值,结合实际生产的可行性将夏枯草种子质量标准初步划分为3个等级。
2.采用不同引发技术处理夏枯草种子,以不同浓度的NaCl模拟盐分胁迫,以不同浓度的PEG模拟干旱胁迫,研究夏枯草种子在逆境条件下的萌发情况,以及种子引发技术对夏枯草种子生活力、耐盐性和抗旱性的影响。随着胁迫强度增大,夏枯草种子萌发受到抑制;适量浓度的GA_3、PEG和KNO_3-KH_2PO_4可提高夏枯草种子活力,增强种子耐盐性以及抗旱性;NaCl引发夏枯草种子活力降低,降低夏枯草种子在盐胁迫和干旱胁迫条件下的萌发率。
3.通过研究夏枯草种子吸水保水特性、种子发芽特性、夏枯草种子粘膜物质对种子发芽的影响等几个方面的内容,发现夏枯草种子粘膜物质具有较强的吸水、保水能力;水分越多,夏枯草种子粘膜层越厚,种子发芽受抑制;夏枯草种子粘膜中含有对种子发芽起促进作用的物质,但种子粘膜层一定程度上阻碍种子发芽;初步探讨了夏枯草种子粘膜的基本生理功能。
Prunella vulgaris L.,plant of its fruit cluster,is a Chinese medicinal material gathering drug,edible,view and admire.
Excellent germplasm is the basis and key to ensure the quality of Chinese medicine material.It is important to study characteristic of germplasm and seed vigor of Chinese medicinal material.This paper studies the quality classification standard of the seed of P.vulgaris from different population,seed priming technology which improves seed vigor, and the characteristic of seed mucilage.The results are as follows:
1.The seed purity,1 000 seeds weight,seed moisture content,seed relative conductivity and seed viability of P.vulgaris of 34 different population were tested and these indexes were analyzed by K clustering.The results showed that the seed purity,1 000 seeds weight,seed moisture content,seed relative conductivity and seed viability of P. vulgaris from different population exist significantly differences.Three final cluster centers were obtained by K clustering analysis.Based on three final clusters of these indexes and practical production,seed quality standard of P.vulgaris were divided into three classes.It was scientific to class the seed qulity standard through mathematical clustering and benefit for good agriclutural practice of P.vulgaris.
2.P.vulgaris seeds were primed with different methods.Salt stress was made by the different concentrations of NaCl solutions,drought stress was made by the different concentrations of polyethylene glycol(PEG).The paper studies seed germination in stress and influence by priming technology on the vigor and stress-resistance of P.vulgaris seeds. As concentrations of stress increasing,seed germination reduced.Primied seeds with PEG, GA_3,KNO_3-KH_2PO_4 under proper concentration,seed vigor,stress-resistance increased while treated with NaCl reduced seed vigor and seed germination percentage in salt and water stress.
3.Hydroscopicity,water holding,germinative condition and the influence of seed mucilage on the germination were tested to primarily elucidate the function of seed mucilage of P.vulgaris.Seed mucilage of P.vulgaris have great ability in absorbing and holding water.The more water,the more seed mucilage formed while the germination of seeds suffer more inhibition.There are some beneficial content in the seed mucilage improving seed germination,while seed mucilage hinder seed of P.vulgaris germination
优良的种质是确保中药材质量的关键,研究药用植物种质特性、种子活力具有十分重要的意义。本文主要研究了夏枯草种子质量标准、提高夏枯草种子活力的引发技术,以及夏枯草种子粘膜的基本特性。研究结果如下:
1.通过对34个居群夏枯草种子进行净度、千粒重、含水量、相对电导率以及发芽检测,各项指标测定结果再进行K聚类分析,结果表明:不同居群夏枯草种子净度、千粒重、含水量、相对电导率、发芽结果差异极显著;K聚类得到各项指标的3个中心值;根据各个指标的中心值,结合实际生产的可行性将夏枯草种子质量标准初步划分为3个等级。
2.采用不同引发技术处理夏枯草种子,以不同浓度的NaCl模拟盐分胁迫,以不同浓度的PEG模拟干旱胁迫,研究夏枯草种子在逆境条件下的萌发情况,以及种子引发技术对夏枯草种子生活力、耐盐性和抗旱性的影响。随着胁迫强度增大,夏枯草种子萌发受到抑制;适量浓度的GA_3、PEG和KNO_3-KH_2PO_4可提高夏枯草种子活力,增强种子耐盐性以及抗旱性;NaCl引发夏枯草种子活力降低,降低夏枯草种子在盐胁迫和干旱胁迫条件下的萌发率。
3.通过研究夏枯草种子吸水保水特性、种子发芽特性、夏枯草种子粘膜物质对种子发芽的影响等几个方面的内容,发现夏枯草种子粘膜物质具有较强的吸水、保水能力;水分越多,夏枯草种子粘膜层越厚,种子发芽受抑制;夏枯草种子粘膜中含有对种子发芽起促进作用的物质,但种子粘膜层一定程度上阻碍种子发芽;初步探讨了夏枯草种子粘膜的基本生理功能。
Prunella vulgaris L.,plant of its fruit cluster,is a Chinese medicinal material gathering drug,edible,view and admire.
Excellent germplasm is the basis and key to ensure the quality of Chinese medicine material.It is important to study characteristic of germplasm and seed vigor of Chinese medicinal material.This paper studies the quality classification standard of the seed of P.vulgaris from different population,seed priming technology which improves seed vigor, and the characteristic of seed mucilage.The results are as follows:
1.The seed purity,1 000 seeds weight,seed moisture content,seed relative conductivity and seed viability of P.vulgaris of 34 different population were tested and these indexes were analyzed by K clustering.The results showed that the seed purity,1 000 seeds weight,seed moisture content,seed relative conductivity and seed viability of P. vulgaris from different population exist significantly differences.Three final cluster centers were obtained by K clustering analysis.Based on three final clusters of these indexes and practical production,seed quality standard of P.vulgaris were divided into three classes.It was scientific to class the seed qulity standard through mathematical clustering and benefit for good agriclutural practice of P.vulgaris.
2.P.vulgaris seeds were primed with different methods.Salt stress was made by the different concentrations of NaCl solutions,drought stress was made by the different concentrations of polyethylene glycol(PEG).The paper studies seed germination in stress and influence by priming technology on the vigor and stress-resistance of P.vulgaris seeds. As concentrations of stress increasing,seed germination reduced.Primied seeds with PEG, GA_3,KNO_3-KH_2PO_4 under proper concentration,seed vigor,stress-resistance increased while treated with NaCl reduced seed vigor and seed germination percentage in salt and water stress.
3.Hydroscopicity,water holding,germinative condition and the influence of seed mucilage on the germination were tested to primarily elucidate the function of seed mucilage of P.vulgaris.Seed mucilage of P.vulgaris have great ability in absorbing and holding water.The more water,the more seed mucilage formed while the germination of seeds suffer more inhibition.There are some beneficial content in the seed mucilage improving seed germination,while seed mucilage hinder seed of P.vulgaris germination
引文
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