摘要
厌氧-厌氧氨氧化组合工艺作为低能耗脱氮工艺,如何提供适宜比例的亚硝酸盐成为研究的关键问题之一.部分反硝化为稳定提供厌氧氨氧化所需的亚硝酸盐提供了可行途径.本文重点针对厌氧工艺中可能产生的中长链脂肪酸对反硝化过程的影响进行研究,筛选出两株具有反硝化能力的细菌Pseudomonas veronii(W-22)和Pseudomonas alcaliphila(W-39),通过批次试验,考察了中长链脂肪酸和常用碳源对菌株反硝化性能的影响.结果表明,在硝酸盐浓度为100 mg·L~(-1),C/N=15,30℃条件下,W-22利用葡萄糖、W-39利用乙醇和葡萄糖,可在36 h内达到稳定的亚硝酸盐累积,亚硝酸盐最大累积速率(R_m)分别为2.50、5.56和8.35 mg·L~(-1)·h~(-1),亚硝酸盐浓度分别维持在57.11、82.14和80.16 mg·L~(-1);W-39利用己酸钠为碳源的R_m为0.99 mg·L~(-1)·h~(-1),亚硝酸盐浓度逐渐升高至72.34 mg·L~(-1);W-22和W-39利用辛酸钠的反应迟滞期在57 h以上,后期伴随硝酸盐浓度降低和亚硝酸盐浓度升高,R_m分别为0.97和7.17 mg·L~(-1)·h~(-1).在本研究条件下,碳源类型对菌株反硝化进程的影响存在差异.
The appropriate nitrite proportion has become one of the key issues for anaerobic-ANAMMOX process, and a feasible way to provide stable nitrite for ANAMMOX is via partial denitrification. This study was investigated on the influence of fatty acids as carbon sources on the denitrification process. Strain W-22 and W-39 with denitrification ability were isolated and were identified as Pseudomonas veronii and Pseudomonas alcaliphila, respectively. Under the condition that initial nitrate concentration was 100 mg·L~(-1), C/N=15 and the operation temperature was 30 ℃, stable nitrite accumulation can be observed within 36 h for W-22 using glucose, and for W-39 using both ethanol and glucose, with the maximum accumulation rate(R_m) of 2.50、5.56 and 8.35 mg·L~(-1)·h~(-1), and the nitrite concentration were 57.11, 82.14 and 82.16 mg·L~(-1),respectively. When sodium caproate was used in W-39, the nitrite concentration gradually increased to 72.34 mg·L~(-1) with the R_m of 0.99 mg·L~(-1)·h~(-1). When sodium caprylate was used for W-22 and W-39, the lag phase was up to 57 h, followed by the nitrite concentration increased with the R_m were 0.97 and 7.17 mg·L~(-1)·h~(-1). The influence of carbon source on denitrification process of bacterial strain was different.
引文
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