Fe~0对污泥接种餐厨垃圾厌氧发酵及抗生素抗性基因的影响
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摘要
在中温条件下以污泥为接种物,以餐厨垃圾为发酵底物,通过向厌氧发酵系统中投加不同量的Fe~0,考察其对餐厨垃圾厌氧发酵过程中日产气量、累积产气量、SCOD、氨氮、挥发性脂肪酸(VFAs)以及厌氧发酵体系中抗生素抗性基因行为特征的影响(包括β-内酰胺类bla OXA-1、bla TEM,大环内脂类erm B、erm F、mef A,磺胺类sul1、sul2,四环素类tet M、tet O、tet Q、tet W、tet X和Ⅰ类整合子int I1)。结果表明:投加Fe~0可以起到提高产气量,促进有机物的水解,缓解酸抑制的作用,当投加20 g/L Fe~0时产气量最高。由于经过湿热水解预处理,原始餐厨垃圾的抗性基因丰度较低,但接种污泥中ermF、tetO、tetQ和tetW的丰度较高,分别为5. 13×10~5,3. 04×10~5,2. 03×10~5,1. 11×10~5copies/g,经厌氧发酵后这4种抗性基因丰度削减明显,但是mefA和tetM的丰度与原始投加物料相比有所增长,投加20 g/L Fe~0与未投加Fe~0相比,mefA、tetM 2种抗性基因的丰度有一定削减。
The anaerobic digestion of food waste was conducted under mesophilic conditions inoculated with wastewater treatment sludge. The influence of Fe~0 on the anaerobic fermentation performance,such as gas production,SCOD,ammonianitrogen,volatile fatty acids( VFAs) and the fate of antibiotic resistance genes( ARGs),was investigated. The ARGs includeβ-lactams ARGs( bla OXA-1,bla TEM),macrolides ARGs( ermB,ermF,mefA),sulfonamides ARGs( sul1,sul2),tetracycline ARGs( tetM,tetO,tetQ,tetW,tetX) and class I integron int I1. The results showed that the Fe~0 could increase the gas production,promote the hydrolysis of organics and alleviate the acid inhibition. When 20 g/L Fe~0 was added in the system,the gas production reached the maximum value. The abundance of ARGs in the original food waste was low due to the hydrothermal pretreatment,but the abundances of erm F,tetO,tetQ and tetW in the sludge were higher,detected as 5. 13 ×10~5,3. 04×10~5,2. 03×10~5 and 1. 11×10~5 copies/g,respectively. The abundance of these four ARGs was significantly reduced after anaerobic fermentation,but the abundance of mef A and tet M increased compared with the initial substrates. Compared with none Fe~0 group,the abundance of mef A and tet M reduced in the system after adding 20 g/L Fe~0.
The anaerobic digestion of food waste was conducted under mesophilic conditions inoculated with wastewater treatment sludge. The influence of Fe~0 on the anaerobic fermentation performance,such as gas production,SCOD,ammonianitrogen,volatile fatty acids( VFAs) and the fate of antibiotic resistance genes( ARGs),was investigated. The ARGs includeβ-lactams ARGs( bla OXA-1,bla TEM),macrolides ARGs( ermB,ermF,mefA),sulfonamides ARGs( sul1,sul2),tetracycline ARGs( tetM,tetO,tetQ,tetW,tetX) and class I integron int I1. The results showed that the Fe~0 could increase the gas production,promote the hydrolysis of organics and alleviate the acid inhibition. When 20 g/L Fe~0 was added in the system,the gas production reached the maximum value. The abundance of ARGs in the original food waste was low due to the hydrothermal pretreatment,but the abundances of erm F,tetO,tetQ and tetW in the sludge were higher,detected as 5. 13 ×10~5,3. 04×10~5,2. 03×10~5 and 1. 11×10~5 copies/g,respectively. The abundance of these four ARGs was significantly reduced after anaerobic fermentation,but the abundance of mef A and tet M increased compared with the initial substrates. Compared with none Fe~0 group,the abundance of mef A and tet M reduced in the system after adding 20 g/L Fe~0.
引文
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[13]魏桃员,温海东,成家杨.零价铁对餐厨垃圾厌氧消化产甲烷的影响研究[J].环境污染与防治,2016,38(12):54-58,64.
[14]张玉静,蒋建国,王佳明.pH值对餐厨垃圾厌氧产酸的影响[J].中国给水排水,2013,29(1):30-34.
[15]李政伟,尹小波,李强,等.氨氮浓度对餐厨垃圾两相发酵中产甲烷相的影响[J].中国沼气,2016,34(1):46-49.
[16]Kong X,Wei Y H,Xu S,et al.Inhibiting excessive acidification using zero-valent iron in anaerobic digestion of food waste at high organic load rates[J].Bioresource Technology,2016,211:65-71.
[17]Gao P,Gu C C,Wei X,et al.The role of zero valent iron on the fate of tetracycline resistance genes and class 1 integrons during thermophilic anaerobic co-digestion of waste sludge and kitchen waste[J].Water Research,2017,111:92-99.
[18]杨帆,徐雯丽,钱雅洁,等.零价铁对污泥厌氧消化过程中四环素抗性基因水平转移的作用影响[J].环境科学,2018,39(4):1748-1755.
[2]王攀,任连海,邱银权,等.利用餐厨垃圾湿热处理脱出液制备液态菌肥研究[J].环境工程,2017,34(6):88-92.
[3]Liao H P,Lu X M,Rensing C,et al.Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge[J].Environmental Science&Technology,2018,52:266-276.
[4]Calero-Cáceres W,Melgarejo A,Colomer-Lluch M,et al.Sludge as a potential important source of antibiotic resistance genes in both the bacterial and bacteriophage fractions[J].Environ Sci Technol,2014,48(13):7602-7611.
[5]Luo G,Li B,Li L G,et al.Antibiotic resistance genes and correlations with microbial community and metal resistance genes in full-scale biogas reactors as revealed by metagenomic analysis[J].Environmetal Science&Technology,2017,51:4069-4080.
[6]Xu F Q,Li Y Y,Ge X M,et al.Anaerobic digestion of food waste-Challenges and opportunities[J].Bioresour Technol,2017,247:1047-1058.
[7]Daniels L,Belay N,Rajagopal B S,et al.Bacterial methanogenesis and growth from CO2with elemental iron as the sole source of electrons[J]Science,1987,237(4814):509-511.
[8]Kong X,Wei Y H,Xu S,et al.Inhibiting excessive acidification using zero-valent iron in anaerobic digestion of food waste at high organic load rates[J].Bioresource Technology,2016,211:65-71.
[9]Gao P,Gu C C,Wei X,et al.The role of zero valent iron on the fate of tetracycline resistance genes and class 1 integrons during thermophilic anaerobic co-digestion of waste sludge and kitchen waste[J].Water Research,2017,111:92-99.
[10]Pu C J,Liu H,Ding G C,et al.Impact of direct application of biogas slurry and residue in fields:in situ analysis of antibiotic resistance genes from pig manure to fields[J].Journal of Hazardous Materials,2018,344:441-449.
[11]Feng Y H,Zhang Y B,Quan X,et al.Enhanced anaerobic digestion of waste activated sludge digestion by the addition of zero valent iron[J].Water Research,2014:52:242-250.
[12]Wei W,Cai Z Q,Fu J,et al.Zero valent iron enhances methane production from primary sludge in anaerobic digestion[J].Chemical Engineering Journal,2018,351:1159-1165.
[13]魏桃员,温海东,成家杨.零价铁对餐厨垃圾厌氧消化产甲烷的影响研究[J].环境污染与防治,2016,38(12):54-58,64.
[14]张玉静,蒋建国,王佳明.pH值对餐厨垃圾厌氧产酸的影响[J].中国给水排水,2013,29(1):30-34.
[15]李政伟,尹小波,李强,等.氨氮浓度对餐厨垃圾两相发酵中产甲烷相的影响[J].中国沼气,2016,34(1):46-49.
[16]Kong X,Wei Y H,Xu S,et al.Inhibiting excessive acidification using zero-valent iron in anaerobic digestion of food waste at high organic load rates[J].Bioresource Technology,2016,211:65-71.
[17]Gao P,Gu C C,Wei X,et al.The role of zero valent iron on the fate of tetracycline resistance genes and class 1 integrons during thermophilic anaerobic co-digestion of waste sludge and kitchen waste[J].Water Research,2017,111:92-99.
[18]杨帆,徐雯丽,钱雅洁,等.零价铁对污泥厌氧消化过程中四环素抗性基因水平转移的作用影响[J].环境科学,2018,39(4):1748-1755.
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