樟树成熟种子超低温保存研究
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摘要
[目的]研究樟树成熟种子保存的最佳方法。[方法]利用正交试验对樟树种子进行超低温保存,探讨樟树成熟种子保存的最佳方法。[结果]樟树种子超低温保存过程中种子最佳含水量为26%;在樟树种子超低温保存过程中,15%二甲基亚砜是最好的冷冻保护剂之一;最利于樟树种子超低温保存的解冻方式是慢冻和慢解冻。[结论]该研究为研究樟树种子低温保存提供理论依据。
[Objective] To study the best method and conditions to protect the seed activity of Cinnamomum camphora Presl in the process of seed preservation. [Method] The seeds of C.camphora were cryopreserved by orthogonal test, and the conductivity of the preserved seeds and the dehydrogenase in vitro were determined. [Result] 26% was the optimal water content of camphor seeds in the process of cryopreservation. 15% dimethyl sulfoxide was one of the best cryoprotectants in the cryopreservation of C.camphora. Slow freezing and slow thawing were the most favorable methods for the cryopreservation of seeds. [Conclusion] This study could provide theoretical basis for the study on the cryopreservation of C.camphora seeds.
[Objective] To study the best method and conditions to protect the seed activity of Cinnamomum camphora Presl in the process of seed preservation. [Method] The seeds of C.camphora were cryopreserved by orthogonal test, and the conductivity of the preserved seeds and the dehydrogenase in vitro were determined. [Result] 26% was the optimal water content of camphor seeds in the process of cryopreservation. 15% dimethyl sulfoxide was one of the best cryoprotectants in the cryopreservation of C.camphora. Slow freezing and slow thawing were the most favorable methods for the cryopreservation of seeds. [Conclusion] This study could provide theoretical basis for the study on the cryopreservation of C.camphora seeds.
引文
[1] 郭玉琼,赖钟雄,吕柳新.玻璃化法超低温保存龙眼胚性愈伤组织的初步探讨[J].福建农林大学学报(自然科学版),2006,35(3):262-265.
[2] 殷晓辉,舒理慧.植物种质资源的超低温保存研究进展(综述)[J].热带亚热带植物学报,1996,4(3):75-82.
[3] 李庆荣,郑郁善.顽拗性种子种质超低温保存研究进展[J].江西农业大学学报,2003,25(4):608-612.
[4] 陈礼光,陈羨德,林国新,等.LN2处理板栗种子和离体胚保存的效果分析[J].江西农业大学学报,2005,27(6):890-894.
[5] 黄学林,陈润政.种子生理实验手册[M].北京:北京大学出版社,1990:125-127.
[6] 张宪政.作物生理研究法[M].北京:农业出版社,1992.
[7] 傅家瑞.种子生理[M].北京:科学出版社,1985.
[8] 彭幼芬,王文章,胡哲森,等.种子生理学[M].长沙:中南工业大学出版社,1994.
[9] 郑郁善,陈礼光,李庆荣,等.板栗种子超低温保存研究[J].林业科学,2002,38(6):146-149.
[10] RUHL G,DAMBROTH M,BIEHL B.Investigations on the causes of sensitivity to cold and drying of tropical seeds with cocoa as an example.II.Dehydration parameters and exudation of drying cocoa seeds and seed organs[J].Landbauforschung volkenrode,1988,38(3):235-251.
[11] PENCE V C.Desiccation and the survival of Aesculus,Castanea and Quercus embryo axes through cryopreservation[J].Cryobiology,1992,29(3):391-399.
[12] FU J R,XIA Q H,TANG F.Effects of desiccation on excised embryonic axes of three recalcitrant seeds and studies on cryopreservation[J].Seed Sci&Technol,1993,21:85-95.
[13] 唐安军,龙春林.低温保存技术在顽拗性种子种质保存中的利用[J].广西植物,2007,27(5):759-764.
[2] 殷晓辉,舒理慧.植物种质资源的超低温保存研究进展(综述)[J].热带亚热带植物学报,1996,4(3):75-82.
[3] 李庆荣,郑郁善.顽拗性种子种质超低温保存研究进展[J].江西农业大学学报,2003,25(4):608-612.
[4] 陈礼光,陈羨德,林国新,等.LN2处理板栗种子和离体胚保存的效果分析[J].江西农业大学学报,2005,27(6):890-894.
[5] 黄学林,陈润政.种子生理实验手册[M].北京:北京大学出版社,1990:125-127.
[6] 张宪政.作物生理研究法[M].北京:农业出版社,1992.
[7] 傅家瑞.种子生理[M].北京:科学出版社,1985.
[8] 彭幼芬,王文章,胡哲森,等.种子生理学[M].长沙:中南工业大学出版社,1994.
[9] 郑郁善,陈礼光,李庆荣,等.板栗种子超低温保存研究[J].林业科学,2002,38(6):146-149.
[10] RUHL G,DAMBROTH M,BIEHL B.Investigations on the causes of sensitivity to cold and drying of tropical seeds with cocoa as an example.II.Dehydration parameters and exudation of drying cocoa seeds and seed organs[J].Landbauforschung volkenrode,1988,38(3):235-251.
[11] PENCE V C.Desiccation and the survival of Aesculus,Castanea and Quercus embryo axes through cryopreservation[J].Cryobiology,1992,29(3):391-399.
[12] FU J R,XIA Q H,TANG F.Effects of desiccation on excised embryonic axes of three recalcitrant seeds and studies on cryopreservation[J].Seed Sci&Technol,1993,21:85-95.
[13] 唐安军,龙春林.低温保存技术在顽拗性种子种质保存中的利用[J].广西植物,2007,27(5):759-764.