基因工程功能化丝蛋白生物材料及其在生物医学中的应用
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摘要
丝蛋白生物材料具有优异的力学性能、良好的生物相容性及可降解性,在生物医学领域具有巨大的应用潜力。现有丝蛋白生物材料在结构和功能方面的相关知识,为设计合成新型丝蛋白生物材料提供了理论基础。此外,利用基因工程技术可将编码新肽或结构域的基因序列添加到编码丝蛋白的基因序列中,以获得具有新功能的丝蛋白生物材料,并更好地满足现代生物医学的需求。文中总结了基因工程功能化的丝蛋白生物材料在生物医学领域中的应用现状和发展前景。
Silk-based biomaterials are featured with excellent mechanical properties, good biocompatibility and biodegradability, which contribute to their potential applications in biomedical field. The current recognition of silk protein materials in structure and function provides a basic theory for the transformation of silk protein into new types of biomaterials.In addition, exogenous sequences encoding new peptide or structural domain can be inserted into the maternal gene sequences encoding silk proteins through genetic engineering technology to synthesize novel silk-based biomaterials with unique functions. This review summarizes the current trend and development perspective of genetically engineered functional silk-based materials for biomedical applications.
Silk-based biomaterials are featured with excellent mechanical properties, good biocompatibility and biodegradability, which contribute to their potential applications in biomedical field. The current recognition of silk protein materials in structure and function provides a basic theory for the transformation of silk protein into new types of biomaterials.In addition, exogenous sequences encoding new peptide or structural domain can be inserted into the maternal gene sequences encoding silk proteins through genetic engineering technology to synthesize novel silk-based biomaterials with unique functions. This review summarizes the current trend and development perspective of genetically engineered functional silk-based materials for biomedical applications.
引文
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[15]Xu M,Lewis RV.Structure of a protein superfiber:spider dragline silk.Proc Natl Acad Sci USA,1990,87(18):7120-7124.
[16]Gaines WA,Sehorn MG,Marcotte WR Jr.Spidroin n-terminal domain promotes a ph-dependent association of silk proteins during self-assembly.JBiol Chem,2010,285(52):40745-40753.
[17]Ittah S,Cohen S,Garty S,et al.An essential role for the C-terminal domain of a dragline spider silk protein in directing fiber formation.Biomacromolecules,2006,7(6):1790-1795.
[18]Qi Y,Wang H,Wei K,et al.A review of structure construction of silk fibroin biomaterials from single structures to multi-level structures.Int J Mol Sci,2017,18(3):237.
[19]Arcidiacono S,Mello C,Kaplan D,et al.Purification and characterization of recombinant spider silk expressed in Escherichia coli.Appl Microbiol Biotechnol,1998,49(1):31-38.
[20]Lewis RV,Hinman M,Kothakota S,et al.Expression and purification of a spider silk protein:a new strategy for producing repetitive proteins.Protein Expr Purif,1996,7(4):400-406.
[21]Tokareva O,Michalczechen-Lacerda VA,Rech EL,et al.Recombinant DNA production of spider silk proteins.Microb Biotechnol,2013,6(6):651-663.
[22]Scheller J,Gührs KH,Grosse F,et al.Production of spider silk proteins in tobacco and potato.Nat Biotechnol,2001,19(6):573-577.
[23]Lazaris A,Arcidiacono S,Huang Y,et al.Spider silk fibers spun from soluble recombinant silk produced in mammalian cells.Science,2002,295(5554):472-476.
[24]Kuwana Y,Sezutsu H,Nakajima K,et al.High-toughness silk produced by a transgenic silkworm expressing spider(Araneus ventricosus)dragline silk protein.PLoS ONE,2014,9(8):e105325.
[25]Fahnestock SR,Bedzyk LA.Production of synthetic spider dragline silk protein in Pichia pastoris.Appl Microbiol Biotechnol,1997,47(1):33-39.
[26]Heidebrecht A,Scheibel T.Recombinant production of spider silk proteins.Adv Appl Microbiol,2013,82:115-153.
[27]Williams D.Sows’ears,silk purses and goats’milk:new production methods and medical applications for silk.Med Device Technol,2003,14(5):9-11.
[28]Xia XX,Qian ZG,Ki CS,et al.Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber.Proc Natl Acad Sci USA,2010,107(32):14059-14063.
[29]Dinjaski N,Kaplan DL.Recombinant protein blends:silk beyond natural design.Curr Opin Biotechnol,2016,39:1-7.
[30]Sutherland TD,Campbell PM,Weisman S,et al.Ahighly divergent gene cluster in honey bees encodes a novel silk family.Genome Res,2006,16(11):1414-1421.
[31]Sezutsu H,Kajiwara H,Kojima K,et al.Identification of four major hornet silk genes with a complex of alanine-rich and serine-rich sequences in Vespa simillima xanthoptera cameron.Biosci Biotechnol Biochem,2007,71(11):2725-2734.
[32]Shi JH,Lua SX,Du N,et al.Identification,recombinant production and structural characterization of four silk proteins from the asiatic honeybee Apis cerana.Biomaterials,2008,29(18):2820-2828.
[33]Weisman S,Haritos VS,Church JS,et al.Honeybee silk:recombinant protein production,assembly and fiber spinning.Biomaterials,2010,31(9):2695-2700.
[34]Sutherland TD,Church JS,Hu XA,et al.Single honeybee silk protein mimics properties of multi-protein silk.PLoS ONE,2011,6(2):e16489.
[35]Krishnaji ST,Bratzel G,Kinahan ME,et al.Sequence-structure-property relationships of recombinant spider silk proteins:integration of biopolymer design,processing,and modeling.Adv Funct Mater,2013,23(2):241-253.
[36]Tokareva OS,Lin SC,Jacobsen MM,et al.Effect of sequence features on assembly of spider silk block copolymers.J Struct Biol,2014,186(3):412-419.
[37]Jastrzebska K,Felcyn E,Kozak M,et al.The method of purifying bioengineered spider silk determines the silk sphere properties.Sci Rep,2016,6:28106.
[38]Lin SC,Ryu S,Tokareva O,et al.Predictive modelling-based design and experiments for synthesis and spinning of bioinspired silk fibres.Nat Commun,2015,6:6892.
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