粤港两地田间发现夜蛾黑卵蜂与螟黄赤眼蜂寄生草地贪夜蛾
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摘要
草地贪夜蛾是新入侵我国的重大危险性害虫,了解野外条件下天敌的发生情况对于挖掘和利用本地天敌资源开展生物防治有重要意义。2019年4月至7月,定期在广州及香港地区的玉米田中开展草地贪夜蛾发生情况调查时,发现野外两种卵寄生蜂寄生草地贪夜蛾。通过分子生物学方法鉴定为夜蛾黑卵蜂和螟黄赤眼蜂,并分别建立实验种群,为人工扩繁及田间应用打下基础,为利用本地天敌开展草地贪夜蛾生物防治提供重要参考。
From April to July, 2019, a regular survey of Spodoptera frugiperda was conducted in the cornfields of Guangzhou and Hong Kong. Two species of egg parasitoids were found to be parasitic in S.frugiperda in the cornfields. Then, the egg parasitoids were cultivated in the eggs of S.frugiperda and identified by molecular identification as Telenomus remus and Trichogramma chilonis. Moreover, the experimental populations of these egg parasitoid wasps were established respectively, which laid a foundation for artificial propagation and field application. This study provides important references for the biological control of S.frugiperda with native natural enemies.
From April to July, 2019, a regular survey of Spodoptera frugiperda was conducted in the cornfields of Guangzhou and Hong Kong. Two species of egg parasitoids were found to be parasitic in S.frugiperda in the cornfields. Then, the egg parasitoids were cultivated in the eggs of S.frugiperda and identified by molecular identification as Telenomus remus and Trichogramma chilonis. Moreover, the experimental populations of these egg parasitoid wasps were established respectively, which laid a foundation for artificial propagation and field application. This study provides important references for the biological control of S.frugiperda with native natural enemies.
引文
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Cave RD.Biology,ecology and use in pest management of Telenomus remus [J].Biocontrol News and Information,2000,21:21-26.
Chen L,Chen KW,Liang GW.Antennal sensilla of female Telenomus remus observed with scanning electron microscopy [J].Journal of South China Agricultural University,2013,34 (1):72-75.[陈丽,陈科伟,梁广文.夜蛾黑卵蜂雌蜂触角感器的扫描电镜观察 [J].华南农业大学学报,2013,34 (1):72-75]
Chen L,Chen KW,Xu ZF,et al.Olfactory response of Telenomus remus Nixon to the information compounds of Spodoptera exigua (Hübner) [J].Journal of Changjiang Vegetables,2010,18:4-7.[陈丽,陈科伟,许再福,等.夜蛾黑卵蜂Telenomus remus Nixon对甜菜夜蛾信息化合物的嗅觉反应 [J].长江蔬菜,2010,18:4-7]
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Feldmann F,Rieckmann U,Winter S.The spread of the fall armyworm Spodoptera frugiperda in Africa:What should be done next?[J].Journal of Plant Diseases and Protection,2019,126 (5):97-101.
Figueiredo MLC,Lucia DT,Cruz I.Effect of Telenomus remus Nixon (Hymenoptera:Scelionidae) density on control of Spodoptera frugiperda (Smith) (Lepidoptera:Noctuidae) egg masses upon release in maize field [J].Revista Brasileira de Milho e Sorgo,2002,1:12-19.
Folmer O,Black M,Hoeh W,et al.DNA primers for amplification of mitochondrial cytochrome coxidase subunit I from diverse metazoan invertebrates [J].Molecular Marine Biology and Biotechnology,1994,3 (5),294-299.
Ge SH,He LM,He W,et al.Determination on moth flight capacity of Spodoptera frugiperda [J/OL].Plant Protection,2019.https://doi.org/10.16688/j.zwbh.2019322 [葛世帅,何莉梅,和伟,等.草地贪夜蛾的飞行能力测定 [J/OL].植物保护,2019.https://doi.org/10.16688/j.zwbh.2019322]
Guo JF,He KL,Wang ZY.Biological characteristics,trend of fall armyworm Spodoptera frugiperda,and the strategy for management of the pest [J].Chinese Journal of Applied Entomology,2019,56 (3):361-369.[郭井菲,何康来,王振营.草地贪夜蛾的生物学特性、发展趋势及防控对策 [J].应用昆虫学报,2019,56 (3):361-369]
Han SC,Chen QX,Li LY.A study on the oviposition synergists for in vitro rearing Trichogramma spp.[J].Entomologia Sinica,1994,1 (4):333-338.
Jiang YY,Liu J,Zhu XM.Occurrence and trend of Spodoptera frugiperda invasion in China [J].China Plant Protection,2019,39 (2):33-35.[姜玉英,刘杰,朱晓明.草地贪夜蛾侵入我国的发生动态和未来趋势分析 [J].中国植保导刊,2019,39 (2):33-35]
Johnson SJ.Migration and the life history strategy of the fall armyworm,Spodoptera frugiperda in the Western Hemisphere [J].International Journal of Tropical Insect Science,1987,8 (4/5/6):543-549.
Li LY,Chen QX,Liu WH.Egg-laying behavior of 12 species of trichogramma and its relationship with success rate in vitro [J].Insect Natural Enemies,1989,11 (1):31-36 [李丽英,陈巧贤,刘文惠.十二种赤眼蜂的产卵行为及其与体外培育成功率的关系 [J].昆虫天敌,1989,11 (1):31-36]
Li ZX,Shen ZR.Application of rDNA-ITS2 to molecular identif ication of Trichogramma spp.[J].Journal of China Agricultural University,2002,7 (5):80-84.[李正西,沈佐锐.rDNA-ITS2应用于赤眼蜂分子鉴定的研究 [J].中国农业大学学报,2002,7 (5):80-84]
Lin NQ.Systematic Studies of Chinese Trichogrammatidae (Hymenoptera:Chalcidoidea) [M].Fuzhou:Fujian Science and Technology Press,1994.[林乃铨.中国赤眼蜂分类(膜翅目:小蜂总科)[M].福州:福建科学技术出版社,1994]
Liu WH,Xie ZN,Xiao GF,et al.In vitro culture of Trichogramma(1):Some problems in culture medium and in vitro culture [J].Journal of Plant Conservation,1979,6 (2):17-24.[刘文惠,谢中能,肖国凡,等.赤眼蜂体外培育研究(一):培养液及其体外培育中的一些问题 [J].植物保护学报,1979,6 (2):17-24]
Lu H,Tang JH,Lv BQ,et al.Recent advances in biological cntrol and invasion risk of Spodoptera frugiperda [J].Chinese Journal of Tropical Crops,2019,40 (6):1237-1244.[卢辉,唐继洪,吕宝乾,等.草地贪夜蛾的生物防治及潜在入侵风险 [J].热带作物学报,2019,40 (6):1237-1244]
Lü X,Han SC,De Clercq P,et al.Orthogonal array design for optimization of an artificial medium for in vitro rearing of Trichogramma dendrolimi [J].Entomologia Experimentalis et Applicata,2014,152:52-60.
Lü X,Han SC,Li J,et al.Effects of cold storage on the quality of Trichogramma dendrolimi Matsumura (Hymenoptera:Trichogrammatidae) reared on artificial medium [J].Pest Management Science,2019,75:1328-1338.
Lü X,Han SC,Li ZG,et al.Biological characters of Trichogramma dendrolimi (Hymenoptera:Trichogrammatidae) reared in vitro versus in vivo for thirty generations [J].Scientific Reports,2017,7:17928.
Molina-Ochoa J,Carpenter JE,Heinrichs EA,et al.Parasitoids and parasites of Spodoptera frugiperda (Lepidoptera:Noctuidae) in the Americas and Caribbean Basin:An inventory [J].Florida Entomologist,2003,86 (3):254-289.
National Agricultural Technology Extension and Service Center.Notification on the occurrence of fall armyworm in China and neighboring countries [R].Plant Disease and Insect Information,2019,No.13.[全国农业技术推广服务中心.我国及周边国家草地贪夜蛾发生危害情况通报 [R].植物病虫情报,2019,13期]
Pomari AF,Bueno AD,Bueno R,et al.Releasing number of Telenomus remus Nixon (Hymenoptera:Platygastridae) against Spodoptera frugiperda Smith (Lepidoptera:Noctuidae) in corn,cotton and soybean [J].Ciência Rural,2013,43:377-382.
Shylesha AN,Jalali SK,Gupta A,et al.Studies on new invasive pest Spodoptera frugiperda (J.E.Smith) (Lepidoptera:Noctuidae) and its natural enemies [J].Journal of Biological Control,2018,32 (3):145-151.
Silva RB,Cruz I,Penteado-Dias AM.First report of Dolichozele koebelei Viereck,1911 (Hymenoptera:Braconidae) on larvae of Spodoptera frugiperda (J.E.Smith,1797) (Lepidoptera:Noctuidae) in maize (Zea mays L.) under different cropping systems [J].Brazilian Journal of Biology,2014,74 (3):218-222.
Tang P,Wang ZZ,Wu Q,et al.The natural enemies of the fall armyworm Spodoptera frugiperda and their application in biological control programs [J].Chinese Journal of Applied Entomology,2019,56 (3):370-381.[唐璞,王知知,吴琼,等.草地贪夜蛾的天敌资源及其生物防治中的应用 [J].应用昆虫学报,2019,56 (3):370-381]
Tang YL,Chen KW,Xu ZF,Study on ontogenesis of Telenomus remus Nixon (Hymenoptera:Scelionidae) [J].Journal of Changjiang Vegetables,2010,18:1-3.[唐雅丽,陈科伟,许再福.夜蛾黑卵蜂(Telenomus remus Nixon)个体发育研究 [J].长江蔬菜,2010,18:1-3]
Todd EL,Poole RW.Keys and illustrations for the armyworm moths of the noctuid genus Spodoptera Guenée from the Western Hemisphere [J].Annals of the Entomological Society of America,1980,73 (6):722-738.
Yang PY,Zhu XM,Guo JF,et al.Strategy and advice for managing the fall armyworm in China [J/OL].Plant Protection,2019.https://doi.org/10.16688/j.zwbh.2019260.[杨普云,朱晓明,郭井菲,王振营.我国草地贪夜蛾的防控对策与建议 [J/OL].植物保护,2019.https://doi.org/10.16688/j.zwbh.2019260]
Yang Y,Han Y,Fang ZH,et al.Effect of host egg age and contact time on the parasitic capacity of Telenomus remus (Hymenoptera:Scelionidae) [J].Chinese Journal of Applied Entomology,2012,49 (6):1490-1495.[杨莹,韩勇,方祝红,等.寄主卵龄和接触时间对夜蛾黑卵蜂寄生能力的影响 [J].应用昆虫学报,2012,49 (6):1490-1495]
Carneiro TR,Fernandes OA,Cruz I.Influence of females intraspecific completion and lack of host on Telenomus remus Nixon (Hymenoptera:Scelionidae) parasitism on Spodoptera frugiperda (Smith) (Lepidoptera:Noctuidae) eggs [J].Revista Brasileira de Entomologia,2009,53 (3):457-460.
Cave RD.Biology,ecology and use in pest management of Telenomus remus [J].Biocontrol News and Information,2000,21:21-26.
Chen L,Chen KW,Liang GW.Antennal sensilla of female Telenomus remus observed with scanning electron microscopy [J].Journal of South China Agricultural University,2013,34 (1):72-75.[陈丽,陈科伟,梁广文.夜蛾黑卵蜂雌蜂触角感器的扫描电镜观察 [J].华南农业大学学报,2013,34 (1):72-75]
Chen L,Chen KW,Xu ZF,et al.Olfactory response of Telenomus remus Nixon to the information compounds of Spodoptera exigua (Hübner) [J].Journal of Changjiang Vegetables,2010,18:4-7.[陈丽,陈科伟,许再福,等.夜蛾黑卵蜂Telenomus remus Nixon对甜菜夜蛾信息化合物的嗅觉反应 [J].长江蔬菜,2010,18:4-7]
Chen L.Study on the Host Adaptability of Telenomus remus and Trichogramma confusum [D].Guangzhou:South China Agricultural University,2011.[陈丽.夜蛾黑卵蜂与拟澳洲赤眼蜂寄主适应性差异研究 [D].广州:华南农业大学,2011]
FAO.Integrated management of the fall armyworm on maize.A guide for Farmer Field Schools in Africa [R].Rome:Food and Agriculture Organization of the United Nations,2018:1-139.
Feldmann F,Rieckmann U,Winter S.The spread of the fall armyworm Spodoptera frugiperda in Africa:What should be done next?[J].Journal of Plant Diseases and Protection,2019,126 (5):97-101.
Figueiredo MLC,Lucia DT,Cruz I.Effect of Telenomus remus Nixon (Hymenoptera:Scelionidae) density on control of Spodoptera frugiperda (Smith) (Lepidoptera:Noctuidae) egg masses upon release in maize field [J].Revista Brasileira de Milho e Sorgo,2002,1:12-19.
Folmer O,Black M,Hoeh W,et al.DNA primers for amplification of mitochondrial cytochrome coxidase subunit I from diverse metazoan invertebrates [J].Molecular Marine Biology and Biotechnology,1994,3 (5),294-299.
Ge SH,He LM,He W,et al.Determination on moth flight capacity of Spodoptera frugiperda [J/OL].Plant Protection,2019.https://doi.org/10.16688/j.zwbh.2019322 [葛世帅,何莉梅,和伟,等.草地贪夜蛾的飞行能力测定 [J/OL].植物保护,2019.https://doi.org/10.16688/j.zwbh.2019322]
Guo JF,He KL,Wang ZY.Biological characteristics,trend of fall armyworm Spodoptera frugiperda,and the strategy for management of the pest [J].Chinese Journal of Applied Entomology,2019,56 (3):361-369.[郭井菲,何康来,王振营.草地贪夜蛾的生物学特性、发展趋势及防控对策 [J].应用昆虫学报,2019,56 (3):361-369]
Han SC,Chen QX,Li LY.A study on the oviposition synergists for in vitro rearing Trichogramma spp.[J].Entomologia Sinica,1994,1 (4):333-338.
Jiang YY,Liu J,Zhu XM.Occurrence and trend of Spodoptera frugiperda invasion in China [J].China Plant Protection,2019,39 (2):33-35.[姜玉英,刘杰,朱晓明.草地贪夜蛾侵入我国的发生动态和未来趋势分析 [J].中国植保导刊,2019,39 (2):33-35]
Johnson SJ.Migration and the life history strategy of the fall armyworm,Spodoptera frugiperda in the Western Hemisphere [J].International Journal of Tropical Insect Science,1987,8 (4/5/6):543-549.
Li LY,Chen QX,Liu WH.Egg-laying behavior of 12 species of trichogramma and its relationship with success rate in vitro [J].Insect Natural Enemies,1989,11 (1):31-36 [李丽英,陈巧贤,刘文惠.十二种赤眼蜂的产卵行为及其与体外培育成功率的关系 [J].昆虫天敌,1989,11 (1):31-36]
Li ZX,Shen ZR.Application of rDNA-ITS2 to molecular identif ication of Trichogramma spp.[J].Journal of China Agricultural University,2002,7 (5):80-84.[李正西,沈佐锐.rDNA-ITS2应用于赤眼蜂分子鉴定的研究 [J].中国农业大学学报,2002,7 (5):80-84]
Lin NQ.Systematic Studies of Chinese Trichogrammatidae (Hymenoptera:Chalcidoidea) [M].Fuzhou:Fujian Science and Technology Press,1994.[林乃铨.中国赤眼蜂分类(膜翅目:小蜂总科)[M].福州:福建科学技术出版社,1994]
Liu WH,Xie ZN,Xiao GF,et al.In vitro culture of Trichogramma(1):Some problems in culture medium and in vitro culture [J].Journal of Plant Conservation,1979,6 (2):17-24.[刘文惠,谢中能,肖国凡,等.赤眼蜂体外培育研究(一):培养液及其体外培育中的一些问题 [J].植物保护学报,1979,6 (2):17-24]
Lu H,Tang JH,Lv BQ,et al.Recent advances in biological cntrol and invasion risk of Spodoptera frugiperda [J].Chinese Journal of Tropical Crops,2019,40 (6):1237-1244.[卢辉,唐继洪,吕宝乾,等.草地贪夜蛾的生物防治及潜在入侵风险 [J].热带作物学报,2019,40 (6):1237-1244]
Lü X,Han SC,De Clercq P,et al.Orthogonal array design for optimization of an artificial medium for in vitro rearing of Trichogramma dendrolimi [J].Entomologia Experimentalis et Applicata,2014,152:52-60.
Lü X,Han SC,Li J,et al.Effects of cold storage on the quality of Trichogramma dendrolimi Matsumura (Hymenoptera:Trichogrammatidae) reared on artificial medium [J].Pest Management Science,2019,75:1328-1338.
Lü X,Han SC,Li ZG,et al.Biological characters of Trichogramma dendrolimi (Hymenoptera:Trichogrammatidae) reared in vitro versus in vivo for thirty generations [J].Scientific Reports,2017,7:17928.
Molina-Ochoa J,Carpenter JE,Heinrichs EA,et al.Parasitoids and parasites of Spodoptera frugiperda (Lepidoptera:Noctuidae) in the Americas and Caribbean Basin:An inventory [J].Florida Entomologist,2003,86 (3):254-289.
National Agricultural Technology Extension and Service Center.Notification on the occurrence of fall armyworm in China and neighboring countries [R].Plant Disease and Insect Information,2019,No.13.[全国农业技术推广服务中心.我国及周边国家草地贪夜蛾发生危害情况通报 [R].植物病虫情报,2019,13期]
Pomari AF,Bueno AD,Bueno R,et al.Releasing number of Telenomus remus Nixon (Hymenoptera:Platygastridae) against Spodoptera frugiperda Smith (Lepidoptera:Noctuidae) in corn,cotton and soybean [J].Ciência Rural,2013,43:377-382.
Shylesha AN,Jalali SK,Gupta A,et al.Studies on new invasive pest Spodoptera frugiperda (J.E.Smith) (Lepidoptera:Noctuidae) and its natural enemies [J].Journal of Biological Control,2018,32 (3):145-151.
Silva RB,Cruz I,Penteado-Dias AM.First report of Dolichozele koebelei Viereck,1911 (Hymenoptera:Braconidae) on larvae of Spodoptera frugiperda (J.E.Smith,1797) (Lepidoptera:Noctuidae) in maize (Zea mays L.) under different cropping systems [J].Brazilian Journal of Biology,2014,74 (3):218-222.
Tang P,Wang ZZ,Wu Q,et al.The natural enemies of the fall armyworm Spodoptera frugiperda and their application in biological control programs [J].Chinese Journal of Applied Entomology,2019,56 (3):370-381.[唐璞,王知知,吴琼,等.草地贪夜蛾的天敌资源及其生物防治中的应用 [J].应用昆虫学报,2019,56 (3):370-381]
Tang YL,Chen KW,Xu ZF,Study on ontogenesis of Telenomus remus Nixon (Hymenoptera:Scelionidae) [J].Journal of Changjiang Vegetables,2010,18:1-3.[唐雅丽,陈科伟,许再福.夜蛾黑卵蜂(Telenomus remus Nixon)个体发育研究 [J].长江蔬菜,2010,18:1-3]
Todd EL,Poole RW.Keys and illustrations for the armyworm moths of the noctuid genus Spodoptera Guenée from the Western Hemisphere [J].Annals of the Entomological Society of America,1980,73 (6):722-738.
Yang PY,Zhu XM,Guo JF,et al.Strategy and advice for managing the fall armyworm in China [J/OL].Plant Protection,2019.https://doi.org/10.16688/j.zwbh.2019260.[杨普云,朱晓明,郭井菲,王振营.我国草地贪夜蛾的防控对策与建议 [J/OL].植物保护,2019.https://doi.org/10.16688/j.zwbh.2019260]
Yang Y,Han Y,Fang ZH,et al.Effect of host egg age and contact time on the parasitic capacity of Telenomus remus (Hymenoptera:Scelionidae) [J].Chinese Journal of Applied Entomology,2012,49 (6):1490-1495.[杨莹,韩勇,方祝红,等.寄主卵龄和接触时间对夜蛾黑卵蜂寄生能力的影响 [J].应用昆虫学报,2012,49 (6):1490-1495]