炼油厂润滑油废白土的微生物修复试验
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摘要
为了寻找一种效率更高的炼油厂润滑油废白土的微生物修复方案,采用3种方案(1~#废白土加营养物,2~#废白土加营养物和强化菌和3~#废白土加营养物、强化菌和土著菌)分别进行修复试验,定期测定不同修复方式下土样中温度、pH、微生物数量和含油量。经过60d处理后,3个试验组降油率分别为42.12%、48.57%和51.79%,表明强化微生物与土著微生物联合处理可以在一定程度上降低润滑油废白土含油量,是一种有效的润滑油废白土处理方法。
In order to find a more efficient microbial remediation scheme for lubricant waste clay in oil refineres,three kinds of schemes(1#waste white soil plus nutrients,2#waste white clay plus nutrients and fortified bacteria,3#waste white clay plus nutrients,fortified bacteria and indigenous bacteria)are used for repair test,and temperature,pH,microbial quantity and oil content are regularly measured.After 60 days of treatment,the oil reduction rates in the three test groups are 42.12%,48.57% and 51.79%,respectively.The results indicate that the combined treatment of strengthening microorganism and indigenous microorganism can reduce the oil content of lubricating oil waste clay to a certain extent,and the combined treatment is an effective way to treat the lubricating oil waste clay.
In order to find a more efficient microbial remediation scheme for lubricant waste clay in oil refineres,three kinds of schemes(1#waste white soil plus nutrients,2#waste white clay plus nutrients and fortified bacteria,3#waste white clay plus nutrients,fortified bacteria and indigenous bacteria)are used for repair test,and temperature,pH,microbial quantity and oil content are regularly measured.After 60 days of treatment,the oil reduction rates in the three test groups are 42.12%,48.57% and 51.79%,respectively.The results indicate that the combined treatment of strengthening microorganism and indigenous microorganism can reduce the oil content of lubricating oil waste clay to a certain extent,and the combined treatment is an effective way to treat the lubricating oil waste clay.
引文
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[3]王文杰,王万福,屈一新,等.润滑油废白土的热解处理[J].环境工程学报,2012,6(6):2067～2071
[4]刘伯约.含油废白土处理工艺探索[D].大连:辽宁师范大学,2014.
[5]张远欣,郭勇,周印生.炼油厂含油废白土选择性脱油工艺小试装置[J].兰州石化职业技术学院学报,2016,16(4):13～15.
[6]闫毓霞.利用土著微生物修复胜利油田含油污泥的工业实验[J].石油与天然气化工,2008,37(3):255～258.
[7]魏小芳,张忠智,郭绍辉,等.外源微生物强化修复石油污染土壤的研究[J].石油化工高等学校学报,2007,20(2):1～4.
[8]Zhan Y B,Tao X L,He S W,et al.Isolation,identification and degradation characteristics of oil degrading bacterial strain[J].Open Access Library Journal,2017,4:1～12.
[9]李宝明,阮志勇,姜瑞波.石油降解菌的筛选、鉴定及菌群构建[J].中国土壤与肥料,2007(3):68～72.
[10]中华人民共和国环境保护部.土壤干物质和水分的测定重量法:HJ 613-2011[S].北京:中国环境科学出版社,2011.
[11]Abioye O P,Alonge O A,Ijah U J J.Biodegradation of crude oil in soil amended with melon shell[J].Au Journal of Technology,2009,13:34～38
[12]赵斌,何绍江.微生物学实验教程[M].北京:高等教育出版社,2013:69～72.
[13]王如刚,王敏,牛晓伟,唐景春.超声-取-重量法测定土壤中总石油烃含量[J].分析化学,2010,38(3):417～420.
[14]Georg F,Matthias B,Johann H.Microbial degradation of aromatic compounds-from one strategy to four[J].Nature Reviews Microbiology,2011,9:803～816.
[15]Ma J,Yang Y,Dai X,et al.Effects of adding bulking agent,inorganic nutrient and microbial inocula on biopile treatment for oil-field drilling waste[J].Chemosphere,2016,150:17～23.