低温胁迫及恢复生长后烟苗叶形指数及生长素的响应
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摘要
为研究低温胁迫及恢复生长后烟苗叶形及生长素含量的动态变化规律,采用盆栽试验研究4℃低温胁迫下及低温后恢复常温生长的烟苗地上部生物量、叶面积、叶形指数、不同部位叶片生长素含量以及生长素外流蛋白家族基因NtPINs表达的变化情况。结果表明,低温胁迫下烟苗地上部生物量较同期对照组极显著减小,恢复常温后虽能继续生长但生物量仍低于同期对照组;低温后恢复生长烟苗的叶形指数极显著高于同期对照组;低温胁迫24 h时,烟苗茎尖新生叶IAA含量较对照组极显著升高,但自下而上第4张叶片的IAA含量较对照组极显著降低,恢复生长8 d时不同部位叶片IAA含量均大幅升高;qRT-PCR结果显示,低温胁迫24 h时,烟苗茎尖新生叶NtPIN1、NtPIN1b、NtPIN3、NtPIN3b、NtPIN4和NtPIN9表达水平均较同期对照极显著下调;低温下施加外源生长素α-萘乙酸(NAA)、生长素极性运输抑制剂1-萘氨甲酰苯甲酸(NPA),常温下施加NPA可明显影响烟苗的叶形指数。因此,低温胁迫导致烟苗地上部生长素从茎尖新生叶向茎基部极性运输的减少是烟苗叶片形态响应低温胁迫的生理机制之一。
引文
[1]胡日生,曾中,戴杏华,等. 不同耐性烟草品种苗期叶片光合特性对低温胁迫的响应[J]. 中国农学通报,2013,29(34):71-75.
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[9]王丰,程方民. 生长素对植物维管分化的信号诱导作用[J]. 江苏林业科技,2004,31(1):40-44.
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[16]赵永长,宋文静,邱春丽,等. 黄腐酸钾对渗透胁迫下烤烟幼苗生长和光合荧光特性的影响[J]. 中国烟草学报,2016,22(4):98-106.
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[18]潘孝武,黎用朝,李小湘,等. CBF调节子在水稻品种日本晴和93-11低温驯化过程中的差异调控机制[J]. 中国水稻科学,2012,26(5):521-528.
[19]Li R Y,Ul-Islam S,Wu Z J,et al. Bensulfuron-methyl treatment of soil affects the infestation of whitefly,aphid,and Tobacco Mosaic virus on Nicotiana tabacum[J]. Frontiers in Plant Science,2016,7:1970.
[20]Meng L,Song W J,Liu S J,et al. Light quality regulates lateral root development in tobacco seedlings by shifting auxin distributions[J]. Journal of Plant Growth Regulation,2015,34(3):574-583.
[21]黄璇. 夜间低温和短时高温对不同烤烟品种生长影响的研究[D]. 长沙:湖南农业大学,2014.
[22]徐静,王莉,钱前,等. 水稻叶片形态建成分子调控机制研究进展[J]. 作物学报,2013,39(5):767-774.
[23]Kim G,Shoda K,Tsuge T,et al. The ANGUSTIFOLIA gene of Arabidopsis,a plant CtBP gene,regulates leaf cell expansion,the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation[J]. EMBO Journal,2002,21(6):1267-1279.
[24]Qiu J L,Jilk R,Marks M D,et al. The Arabidopsis SPIKE1 gene is required for normal cell shape control and tissue development[J]. Plant Cell,2002,14(1):101-118.
[25]Horiguchi G,Kim G T,Tsukaya H. The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana[J]. Plant Journal,2005,43(1):68-78.
[26]Himanen K,Adem D,Lijsebettens V. Genetic and epigenetic control of leaf size and shape[J]. International Journal of Developmental Biology,2007,1(2):226-238.
[27]Wang Q N,An B,Wei Y X,et al. Melatonin regulates root meristem by repressing auxin synthesis and polar auxin transport in Arabidopsis[J]. Frontiers in Plant Science,2016,7:1882.
[28]Shi J M,Dong J Q,Xue J,et al. Model for the role of auxin polar transport in patterning of the leaf adaxial-abaxial axis[J]. Plant Journal,2017,92(3):469-480.
[29]Shibasaki K,Uemura M,Tsurumi S,et al. Auxin response in Arabidopsis under cold stress:underlying molecular mechanisms[J]. Plant Cell,2009,21(12):3823-3838.
[30]Dharmasiri S,Swarup R,Mockaitis K,et al. AXR4 is required for localization of the auxin influx facilitator AUX1[J]. Science,2006,312(5777):1218-1220.
[31]Leyser O,Day S. Mechanisms in plant development[M]. Blackwell:Oxford,2003.
[32]Friml J. Subcellular trafficking of PIN auxin efflux carriers in auxin transport[J]. European Journal of Cell Biology,2010,89(2/3):231-235.
[33]Scarpella E,Barkoulas M,Tsiantis M. Control of leaf and vein development by auxin[J]. Cold Spring Harbor Perspectives in Biology,2010,2(1):137-153.
[2]崔晓峰,黄海. 叶发育的遗传调控机理研究进展[J]. 植物生理学报,2011,47(7):631-640.
[3]Bowman J L,Eshed Y,Baum S F. Establishment of polarity in angiosperm lateral organs[J]. Trends in Genetics,2002,18(3):134-141.
[4]Tsukaya H. Mechanism of leaf-shape determination[J]. Annual Review of Plant Biology,2006,57:477-496.
[5]田小霞,孟林,毛培春,等. 低温条件下不同抗寒性薰衣草内源激素的变化[J]. 植物生理学报,2014(11):1669-1674.
[6]李静,崔继哲,弭晓菊. 生长素与植物逆境胁迫关系的研究进展[J]. 生物技术通报,2012(6):13-17.
[7]Tsukaya H. Leaf morphogenesis:genetic regulations for length,width and size of leaves[J]. Tanpakushitsu Kakusan koso. Protein,Nucleic Acid,Enzyme,2002,47(12):1576-1580.
[8]李林川,瞿礼嘉. 生长素对拟南芥叶片发育调控的研究进展[J]. 植物学通报,2006,23(5):459-465.
[9]王丰,程方民. 生长素对植物维管分化的信号诱导作用[J]. 江苏林业科技,2004,31(1):40-44.
[10]任怡怡,戴绍军,刘炜. 生长素的运输及其在信号转导及植物发育中的作用[J]. 生物技术通报,2012(3):9-16.
[11]Fujino K,Matsuda Y,Ozawa K,et al. NARROW LEAF 7 controls leaf shape mediated by auxin in rice[J]. Molecular Genetics and Genomics,2008,279(5):499-507.
[12]赵阳,王树声,张亚丽,等. 增加烟草一级和二级侧根是抵御干旱的生理机制[J]. 植物营养与肥料学报,2017,23(2):548-555.
[13]刘尚俊. 生长素参与钾胁迫下烟草侧根生长发育机制研究[D]. 南京:南京农业大学,2015.
[14]韩锦峰,岳彩鹏,刘华山,等. 烤烟生长发育的低温诱导研究 Ⅰ. 苗期低温诱导对烤烟顶芽发育及激素含量的影响[J]. 中国烟草学报,2002,8(1):25-29.
[15]李琦瑶,王树声,周培禄,等. 低温胁迫对烟苗叶形及生理特性的影响[J]. 中国烟草科学,2018,39(1):17-23.
[16]赵永长,宋文静,邱春丽,等. 黄腐酸钾对渗透胁迫下烤烟幼苗生长和光合荧光特性的影响[J]. 中国烟草学报,2016,22(4):98-106.
[17]Song W J,Sun H W,Li J,et al. Auxin distribution is differentially affected by nitrate in roots of two rice cultivars differing in responsiveness to nitrogen[J]. Annals of Botany,2013,112(7):1383-1393.
[18]潘孝武,黎用朝,李小湘,等. CBF调节子在水稻品种日本晴和93-11低温驯化过程中的差异调控机制[J]. 中国水稻科学,2012,26(5):521-528.
[19]Li R Y,Ul-Islam S,Wu Z J,et al. Bensulfuron-methyl treatment of soil affects the infestation of whitefly,aphid,and Tobacco Mosaic virus on Nicotiana tabacum[J]. Frontiers in Plant Science,2016,7:1970.
[20]Meng L,Song W J,Liu S J,et al. Light quality regulates lateral root development in tobacco seedlings by shifting auxin distributions[J]. Journal of Plant Growth Regulation,2015,34(3):574-583.
[21]黄璇. 夜间低温和短时高温对不同烤烟品种生长影响的研究[D]. 长沙:湖南农业大学,2014.
[22]徐静,王莉,钱前,等. 水稻叶片形态建成分子调控机制研究进展[J]. 作物学报,2013,39(5):767-774.
[23]Kim G,Shoda K,Tsuge T,et al. The ANGUSTIFOLIA gene of Arabidopsis,a plant CtBP gene,regulates leaf cell expansion,the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation[J]. EMBO Journal,2002,21(6):1267-1279.
[24]Qiu J L,Jilk R,Marks M D,et al. The Arabidopsis SPIKE1 gene is required for normal cell shape control and tissue development[J]. Plant Cell,2002,14(1):101-118.
[25]Horiguchi G,Kim G T,Tsukaya H. The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana[J]. Plant Journal,2005,43(1):68-78.
[26]Himanen K,Adem D,Lijsebettens V. Genetic and epigenetic control of leaf size and shape[J]. International Journal of Developmental Biology,2007,1(2):226-238.
[27]Wang Q N,An B,Wei Y X,et al. Melatonin regulates root meristem by repressing auxin synthesis and polar auxin transport in Arabidopsis[J]. Frontiers in Plant Science,2016,7:1882.
[28]Shi J M,Dong J Q,Xue J,et al. Model for the role of auxin polar transport in patterning of the leaf adaxial-abaxial axis[J]. Plant Journal,2017,92(3):469-480.
[29]Shibasaki K,Uemura M,Tsurumi S,et al. Auxin response in Arabidopsis under cold stress:underlying molecular mechanisms[J]. Plant Cell,2009,21(12):3823-3838.
[30]Dharmasiri S,Swarup R,Mockaitis K,et al. AXR4 is required for localization of the auxin influx facilitator AUX1[J]. Science,2006,312(5777):1218-1220.
[31]Leyser O,Day S. Mechanisms in plant development[M]. Blackwell:Oxford,2003.
[32]Friml J. Subcellular trafficking of PIN auxin efflux carriers in auxin transport[J]. European Journal of Cell Biology,2010,89(2/3):231-235.
[33]Scarpella E,Barkoulas M,Tsiantis M. Control of leaf and vein development by auxin[J]. Cold Spring Harbor Perspectives in Biology,2010,2(1):137-153.