1株耐硒凝结芽孢杆菌的分离鉴定及其培养条件的优化
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摘要
用含硒培养基,采用平板稀释分离法,从湖北恩施渔塘坝高硒环境中分离鉴定1株凝结芽孢杆菌。为确定该凝结芽孢杆菌培养基及培养条件,采用单因素和正交试验法对凝结芽孢杆菌的摇瓶发酵培养条件(碳源、氮源、温度和转速)进行了优化,为确定其培养时间作出了生长曲线。结果表明,温度对凝结芽孢杆菌生长的影响最大,其后依次为氮源、碳源和转速;最适培养基为:葡萄糖11 g/L,牛肉浸膏11 g/L,氯化钠5 g/L;最适培养条件为:温度35℃,转速160 r/min;(2)从生长曲线可看出,0～1.5 h为停滞期,1.5～10 h为对数生长期,10～20 h为稳定期,最佳培养时间为10 h。
引文
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[6]Siddique T,Arocena J M,Thring R W,et al. Bacterial reduction of selenium in coal mine tailings pond sediment[J]. Journal of Environmental Quality,2007,36(3):621-627.
[7]Zhang Y,Okeke B C,Frankenberger W T . Bacterial reduction of selenate to elemental selenium utilizing molasses as a carbon source[J]. Bioresour Technol,2008,99(5):1267-1273.
[8]Morita M,Uemoto H,Watanabe A . Reduction of selenium oxyanions in Wastewater using two bacterial strains[J]. Engineering in Life Sciences,2007,7(3):235-240.
[9]Burton G A,Giddings T H,de Brine P,et al. High incidence of selenite-resistant bacteria from a site polluted with selenium[J]. Applied and Environmental Microbiology,1987,53(1):185-188.
[10]东秀珠,蔡妙英. 常用细菌鉴定手册[M]. 科学出版社,2001:62-65.
[11]董惠钧,姜俊云,郑立军,等. 新型微生态益生菌凝结芽孢杆菌研究进展[J]. 食品科学,2010,31(1):292-294.
[12]Hyronimus B,Le Marrec C,Hadj S A,et al. Acid and bile tolerance of spore-forming lactic acid bacteria[J]. International Journal of Food Microbiology,2000,61(2):193-197.
[13]丁贤,李卓佳,陈永青,等. 芽孢杆菌对凡纳对虾生长和消化酶活性的影响[J]. 中国水产科学,2004,(6):580-584.
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[15]Lin S,Mao S,Guan Y,et al. Effects of dietary chitosan oligosaccharides and Bacillus coagulans on the growth,innate immunity and resistance of koi (Cyprinus carpio Koi)[J]. Aquaculture,2012,342/343:36-41.
[16]王霖,王文杰,刘洋,等. 凝结芽孢杆菌TBC-169菌株治疗抗原诱导大鼠结肠炎的实验研究[J]. 胃肠病学,2008,13(6):349-353.
[17]Dolin B J. Effects of a proprietary Bacillus coagulans preparation on symptoms of diarrhea- predominant irritable bowel syndrome[J]. Methods and Findings in Experimental and Clinical Pharmacology,2009,31(10):655-659.
[18]Mohapatra S,Chakraborty T,Prusty A K,et al. Use of different microbial probiotics in the diet of rohu,Labeo rohita fingerlings:effects on growth,nutrient digestibility and retention,digestive enzyme activities and intestinal microflora[J]. Aquaculture Nutrition,2012,18(1):1-11.