宁夏回族自治区嫁接西瓜发生黄瓜绿斑驳花叶病毒病
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摘要
黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)是我国重要的检疫性病毒。2018年宁夏回族自治区中卫市种植的西瓜部分发生了严重倒瓤,通过实地调研,对发病西瓜植株的叶片和果实症状进行现场观察,初步怀疑为CGMMV侵染所致。对采集的3份西瓜病样采用ELISA(Enzyme Linked Immuno-Sorbnent Assay)和RT-PCR(Reverse Transcription PCR)检测,以及克隆的外壳蛋白基因(CP)序列分析,结果表明,3份样品均感染了CGMMV,证实宁夏回族自治区西瓜发生倒瓤是由CGMMV引起,这也是首次发现CGMMV在宁夏发生。
Cucumber green mottle mosaic virus(CGMMV) is an important quarantine virus in China. In 2018, the watermelon plants in Ningxia Hui autonomous region was seriously infected. Through field investigation, it was initially suspected to be caused by the infection of the virus by the observation of the symptoms of watermelon leaves and fruits.For the detection of the virus, ELISA(Enzym Linked Immuno-Sorbent Assay) and RT-PCR(Reverse Transcription Polymerase Chain Reaction) detection and sequence analysis were performed on three watermelon samples. The results showed that all the three samples were infected with CGMMV, which confirmed that watermelon fruit blood flesh in Ningxia Hui autonomous region was caused by CGMMV. The virus was first reported in Ningxia.
Cucumber green mottle mosaic virus(CGMMV) is an important quarantine virus in China. In 2018, the watermelon plants in Ningxia Hui autonomous region was seriously infected. Through field investigation, it was initially suspected to be caused by the infection of the virus by the observation of the symptoms of watermelon leaves and fruits.For the detection of the virus, ELISA(Enzym Linked Immuno-Sorbent Assay) and RT-PCR(Reverse Transcription Polymerase Chain Reaction) detection and sequence analysis were performed on three watermelon samples. The results showed that all the three samples were infected with CGMMV, which confirmed that watermelon fruit blood flesh in Ningxia Hui autonomous region was caused by CGMMV. The virus was first reported in Ningxia.
引文
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[7]王峰,任春梅,季英华,等.黄瓜绿斑驳花叶病毒海南分离物基因组测定与毒源分析[J].植物保护,2014,40(6):75-81.
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[11]古勤生,彭斌,刘珊珊,等.我国嫁接西瓜黄瓜绿斑驳花叶病毒的防控对策[J].中国蔬菜,2013(11):5-7.
[12]李帅,单文书,冯明峰,等.来自安徽不同地区黄瓜绿斑驳花叶病毒(CGMMV)cp基因的分子进化分析[J].安徽农业大学学报,2016,43(4):593-597.
[2] OKADA Y.Cucumber green mottle mosaic virus[M]//The plant viruses.Springer,Boston,MA,1986:267-281.
[3]李俊香,古勤生.黄瓜绿斑驳花叶病毒传播方式的研究进展[J].中国蔬菜,2015(1):13.
[4] DOMBROVSKY A,TRAN-NGUYEN L T T,JONES R A C.Cucumber green mottle mosaic virus:rapidly increasing global distribution,etiology,epidemiology,and management[J].Annual Review of Phytopathology,2017,55:231-256.
[5]古勤生.防治监控黄瓜绿斑驳花叶病毒,确保瓜类安全生产[J].中国瓜菜,2007,20(1):47-48.
[6]蔡明,金学典.辽宁省西瓜倒瓤病的检疫与防控措施[J].中国植保导刊,2013,33(3):56-57.
[7]王峰,任春梅,季英华,等.黄瓜绿斑驳花叶病毒海南分离物基因组测定与毒源分析[J].植物保护,2014,40(6):75-81.
[8]宋顺华,吴萍,宫国义,等.黄瓜绿斑驳花叶病的发生及防控策略[J].植物保护,2014(8):105-108.
[9]周玲玲,吴元华,赵秀香,等.黄瓜绿斑驳花叶病毒生物学特性及对西瓜生长的影响[J].沈阳农业大学学报,2008,39(4):417-422.
[10]吴会杰,秦碧霞,陈红运,等.黄瓜绿斑驳花叶病毒西瓜、甜瓜种子的带毒率和传毒率[J].中国农业科学,2011,44(7):1527-1532.
[11]古勤生,彭斌,刘珊珊,等.我国嫁接西瓜黄瓜绿斑驳花叶病毒的防控对策[J].中国蔬菜,2013(11):5-7.
[12]李帅,单文书,冯明峰,等.来自安徽不同地区黄瓜绿斑驳花叶病毒(CGMMV)cp基因的分子进化分析[J].安徽农业大学学报,2016,43(4):593-597.