18%四氟·嘧菌酯悬乳剂的高效液相色谱分析
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摘要
采用反相高效液相色谱法,以甲醇+水为流动相,使用ODS-3 C_(18)色谱柱和二极管阵列检测器,在225 nm波长下对18%四氟·嘧菌酯悬乳剂进行分离和定量分析。四氟醚唑和嘧菌酯的线性相关系数分别为0.999 5和0.999 9,变异系数分别为0.16%和0.06%,平均回收率分别为99.99%和99.56%。该分析方法简便、准确,满足定量分析要求。
An analytical method for detection of tetraconazole +azoxystrobin 18% SE was established by HPLC.Methanol and water were used as mobile phase, ODS-3 C_(18) column was chromatographic column, and UV detection wavelength was 225 nm. The results showed the linear correlation coefficients of tetraconazole and azoxystrobin were0.999 5 and 0.999 9, the variation coefficients were 0.16% and 0.06%, and average recoveries were 99.99% and 99.56%,respectively. This method was convenient, accurate and suitable for quantitative analysis of tetraconazole +azoxystrobin18% SE.
An analytical method for detection of tetraconazole +azoxystrobin 18% SE was established by HPLC.Methanol and water were used as mobile phase, ODS-3 C_(18) column was chromatographic column, and UV detection wavelength was 225 nm. The results showed the linear correlation coefficients of tetraconazole and azoxystrobin were0.999 5 and 0.999 9, the variation coefficients were 0.16% and 0.06%, and average recoveries were 99.99% and 99.56%,respectively. This method was convenient, accurate and suitable for quantitative analysis of tetraconazole +azoxystrobin18% SE.
引文
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[2]贾丽,何玲,王李斌,等.嘧菌酯在我国登记情况以及抗性研究现状[J].农药科学与管理, 2014, 35(10):19-23.
[3]段亚玲,蔡磊明,王捷,等.四氟醚唑在土壤中残留的气相色谱分析[J].农药, 2010, 49(4):284-286.
[4]王旭,于福利,雷琪,等.四氟醚唑4%水乳剂气相色谱分析[J].农药科学与管理, 2013, 34(6):36-38.
[5]薄海波,毕阳,陈立仁.气相色谱法测定甜瓜和苹果中嘧菌酯残留[J].分析化学, 2005, 33(5):695-698.
[6]林童,魏玉海,凤晓博,等.超高效液相色谱-串联质谱法测定枸杞中常见的5种农药残留[J].现代农药, 2015(1):37-40.