甜高粱秆发酵渣中可溶性糖和乙醇的快速浸提
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摘要
目的建立一种甜高粱秆发酵渣中可溶性糖和乙醇的快速浸提方法。方法考察高速匀浆时间和后续是否超声振荡对甜高粱渣中可溶性糖和乙醇浸提效率的影响;采用扫描电镜观察甜高粱渣的形态,从细胞层面解释颗粒尺寸对浸提速度的影响;对浸提方法进行加标回收验证。结果浸提方法以高速匀浆3min为宜,在这个条件下,蔗糖和乙醇在低、中、高3个水平的加标回收率在90.17%～92.80%之间。结论该浸提方法可靠,操作简单,浸提时间短,可用于甜高粱秆发酵渣中可溶性糖和乙醇的快速浸提。
Objective To establish a rapid extraction method of soluble sugar and ethanol from sweet sorghum stalk bagasses. Methods The effects of high-speed homogenization time and subsequent ultrasonic on the extraction efficiency were investigated. The morphology of sweet sorghum stalk bagasses was observed by scanning electron microscopy, and the effect of particle size on the extraction efficiency was illustrated in the cellular level. The extraction method was verified by experiment of recovery. Results The results showed that the optimum extraction method was high speed homogenization for 3 min. Under this condition, the recoveries of sucrose and ethanol at low, medium and high spiked levels were ranged from 90.17% to 92.80%. Conclusion The method is reliable, simple and short in extraction time, which can be used for rapid extraction of soluble sugar and ethanol from sweet sorghum stalk bagasses.
Objective To establish a rapid extraction method of soluble sugar and ethanol from sweet sorghum stalk bagasses. Methods The effects of high-speed homogenization time and subsequent ultrasonic on the extraction efficiency were investigated. The morphology of sweet sorghum stalk bagasses was observed by scanning electron microscopy, and the effect of particle size on the extraction efficiency was illustrated in the cellular level. The extraction method was verified by experiment of recovery. Results The results showed that the optimum extraction method was high speed homogenization for 3 min. Under this condition, the recoveries of sucrose and ethanol at low, medium and high spiked levels were ranged from 90.17% to 92.80%. Conclusion The method is reliable, simple and short in extraction time, which can be used for rapid extraction of soluble sugar and ethanol from sweet sorghum stalk bagasses.
引文
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[3] Wang F, Liu CZ. Development of an economic refining strategy of sweet sorghum in the inner Mongolia region of China[J]. Energ Fuels, 2009,23(8):4137-4142.
[4]张继澍.植物生理学[M].北京:高等教育出版社, 2006.Zhang JS. Plant physiology[M]. Beijing:Higher Education Press, 2006.
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[8] GB/T 10499-2014糖料甘蔗试验方法[S].GB/T 10499-2014 Test methods of sugarcane[S].
[9] GB/T 10496-2002糖科甜菜[S].GB/T 10496-2002 Sugar beet[S].
[10]范桂芳,李佩佩,齐立松,等.固体发酵物料可溶性糖和乙醇检测方法研究进展[J].生物产业技术, 2018, 3:50-54.Fan GF, Li PP, Qi LS, et al. Progress on soluble sugars and ethanol determination in solid state fermentation material[J]. Biotechnol Business,2018, 3:50-54.
[11] Zhang Y, Li HF, Ma Y, et al. Microwave assisted extraction-solid phase extraction for high-efficient and rapid analysis of monosaccharides in plants[J]. Talanta, 2014, 129:404-410.
[12]章莹颖,邓春芳,崔岩,等.不同方法对微藻细胞破碎及油脂提取效果的影响[J].中国油脂, 2016, 41(3):61-65.Zhang YY, Deng CF, Cui Y, et al. Effect of different methods on cell disruption and oil extraction of microalgae[J]. China Oils Fats, 2016,41(3):61-65.
[13] Crepeau M, Khelifi M, Vanasse A, et al. Extraction of the sugary juice from sweet pearl millet and sweet sorghum using a hydraulic press and a four-roller press[J]. Trans ASABE, 2016, 59(5):1127-1135.
[14] Aragon D, Viator HP, Ehrenhauser FS. Assessment of sugarcane billet harvester on recovery of sweet sorghum biomass for ethanol production[J]. Bio Energ Res, 2017, 10(3):783-791.
[15] Mao YY, Li JH, Li SZ, et al. The mass transfer of sugar in sweet sorghum stalks for solid-state fermentation process[J]. Fuel, 2015, 144:90-95.
[16] Jia F, Chawhuaymak J, Riley MR, et al. Efficient extraction method to collect sugar from sweet sorghum[J]. J Biol Eng, 2013, 7(1):1.
[17]丛靖宇,刘伟,张新,等.利用高效液相色谱法检定甜高粱茎秆糖分的方法研究[J].内蒙古农业大学学报(自然科学版), 2013, 34(4):80-85.Cong JY, Liu W, Zhang X. Study on extracting sugar of sweet sorghum stalk with HPLC[J]. J Inner Mongolia Agric Univ, 2013, 34(4):80-85.
[18] Saied N, Khelifi M, Aider M, et al. Water-soluble carbohydrate extraction from sweet pearl millet and sweet sorghum biomass as affected by bagasse impregnation[J]. Trans ASABE, 2017, 60(2):253-261.
[19] Gaines TP, Gascho GJ. Automated-determination of total sugars in sugar crops grown for energy[J]. J Sci Food Agric, 1985, 36(3):157-161.
[2] Li SZ, Li GM, Zhang L, et al. A demonstration study of ethanol production from sweet sorghum stems with advanced solid state fermentation technology[J]. Appl Energy, 2013, 102:260-265.
[3] Wang F, Liu CZ. Development of an economic refining strategy of sweet sorghum in the inner Mongolia region of China[J]. Energ Fuels, 2009,23(8):4137-4142.
[4]张继澍.植物生理学[M].北京:高等教育出版社, 2006.Zhang JS. Plant physiology[M]. Beijing:Higher Education Press, 2006.
[5]田瑞华.生物分离工程[M].北京:科学出版社, 2008.Tian RH. Bioseparation engineering[M]. Beijing:Science Press, 2008.
[6]欧阳平凯,胡永红,姚忠.生物分离原理及技术[M].北京:化学工业出版社, 2010.Ouyang PK, Hu YH, Yao Z. Bioseparation principle and technology[M].Beijing:Chemical Industry Press, 2010.
[7]谭天伟.生物分离技术[M].北京:化学工业出版社, 2007.Tan TW. Bioseparation technology[M]. Beijing:Chemical Industry Press,2007.
[8] GB/T 10499-2014糖料甘蔗试验方法[S].GB/T 10499-2014 Test methods of sugarcane[S].
[9] GB/T 10496-2002糖科甜菜[S].GB/T 10496-2002 Sugar beet[S].
[10]范桂芳,李佩佩,齐立松,等.固体发酵物料可溶性糖和乙醇检测方法研究进展[J].生物产业技术, 2018, 3:50-54.Fan GF, Li PP, Qi LS, et al. Progress on soluble sugars and ethanol determination in solid state fermentation material[J]. Biotechnol Business,2018, 3:50-54.
[11] Zhang Y, Li HF, Ma Y, et al. Microwave assisted extraction-solid phase extraction for high-efficient and rapid analysis of monosaccharides in plants[J]. Talanta, 2014, 129:404-410.
[12]章莹颖,邓春芳,崔岩,等.不同方法对微藻细胞破碎及油脂提取效果的影响[J].中国油脂, 2016, 41(3):61-65.Zhang YY, Deng CF, Cui Y, et al. Effect of different methods on cell disruption and oil extraction of microalgae[J]. China Oils Fats, 2016,41(3):61-65.
[13] Crepeau M, Khelifi M, Vanasse A, et al. Extraction of the sugary juice from sweet pearl millet and sweet sorghum using a hydraulic press and a four-roller press[J]. Trans ASABE, 2016, 59(5):1127-1135.
[14] Aragon D, Viator HP, Ehrenhauser FS. Assessment of sugarcane billet harvester on recovery of sweet sorghum biomass for ethanol production[J]. Bio Energ Res, 2017, 10(3):783-791.
[15] Mao YY, Li JH, Li SZ, et al. The mass transfer of sugar in sweet sorghum stalks for solid-state fermentation process[J]. Fuel, 2015, 144:90-95.
[16] Jia F, Chawhuaymak J, Riley MR, et al. Efficient extraction method to collect sugar from sweet sorghum[J]. J Biol Eng, 2013, 7(1):1.
[17]丛靖宇,刘伟,张新,等.利用高效液相色谱法检定甜高粱茎秆糖分的方法研究[J].内蒙古农业大学学报(自然科学版), 2013, 34(4):80-85.Cong JY, Liu W, Zhang X. Study on extracting sugar of sweet sorghum stalk with HPLC[J]. J Inner Mongolia Agric Univ, 2013, 34(4):80-85.
[18] Saied N, Khelifi M, Aider M, et al. Water-soluble carbohydrate extraction from sweet pearl millet and sweet sorghum biomass as affected by bagasse impregnation[J]. Trans ASABE, 2017, 60(2):253-261.
[19] Gaines TP, Gascho GJ. Automated-determination of total sugars in sugar crops grown for energy[J]. J Sci Food Agric, 1985, 36(3):157-161.
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