MBBR-MBR组合工艺处理生活污水试验中微生物种群结构的演变
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摘要
采用移动床生物膜反应器(MBBR)-膜生物反应器(MBR)组合工艺进行生活污水处理试验,考察了组合工艺对污染物的去除效果,通过变性梯度凝胶电泳(DGGE)分析反应器内微生物种群组成及多样性变化,利用克隆文库分析微生物种群结构。结果表明:MBBR-MBR组合工艺稳定运行时对氨氮(NH4+-N)和化学需氧量(COD_(Cr))的平均去除率高达98%和97%;DGGE指纹图谱显示,反应器运行过程中微生物群落结构演变明显且与温度变化密切相关,逐渐形成适应污水处理的低温稳定种群,MBBR与MBR相比,其反应器内微生物种群更丰富,抗冲击负荷能力更强;克隆文库结果显示,MBBR与MBR内微生物优势种有较大差异,但同属变形菌门(Proteobacteria)。
The moving bed biofilm reactor( MBBR)-membrane bioreactor( MBR) combined process was used for the treatment of domestic wastewater. The removal effect of the reactors on the pollutants was investigated, the microbial population changes and compositional diversity in the reactors were analyzed by denaturing gradient gel electrophoresis( DGGE), and the microorganisms were classified by using cloned library. The results showed that the NH4+-N and COD_(Cr)in MBBR-MBR could acquire satisfied removal efficiency under stable operation, which were 98% and 97%. DGGE fingerprint showed that during the operation of the reactor, the microorganism community structure evolved obviously and was closely related to the temperature change, gradually forming a low temperature stable population adapted to the sewage treatment. Compared with MBR, MBBR had richer microbial population, more complex system and stronger resistance. The results of cloned library showed that the dominant species of microorganisms in MBBR and MBR were quite different, but both belonged to Proteobacteria.
The moving bed biofilm reactor( MBBR)-membrane bioreactor( MBR) combined process was used for the treatment of domestic wastewater. The removal effect of the reactors on the pollutants was investigated, the microbial population changes and compositional diversity in the reactors were analyzed by denaturing gradient gel electrophoresis( DGGE), and the microorganisms were classified by using cloned library. The results showed that the NH4+-N and COD_(Cr)in MBBR-MBR could acquire satisfied removal efficiency under stable operation, which were 98% and 97%. DGGE fingerprint showed that during the operation of the reactor, the microorganism community structure evolved obviously and was closely related to the temperature change, gradually forming a low temperature stable population adapted to the sewage treatment. Compared with MBR, MBBR had richer microbial population, more complex system and stronger resistance. The results of cloned library showed that the dominant species of microorganisms in MBBR and MBR were quite different, but both belonged to Proteobacteria.
引文
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[26]王萍,余志晟,齐嵘,等.丝状细菌污泥膨胀的FISH探针研究进展[J].应用与环境生物学报,2012,18(4):705-712.WANG P,YU Z S,QI R,et al. Progress on FISH probes of sludge bulking caused by filamentous bacteria[J]. Chinese Journal of Applied&Environmental Biology,2012,18(4):705-712.
[2] HOSSEINI KOUPAIE E,ALAVI MOGHADDAM M R,HASHEMI S H. Post-treatment of anaerobically degraded azo dye Acid Red 18 using aerobic moving bed biofilm process:enhanced removal of aromatic amines[J]. Journal of Hazardous Materials,2011,195:147-154.
[3] WELLS G F,SHI Y J,JOSS A,et al. Comparing process performance and stability between replicate MBBR and suspended growth combined nitritation-anammox reactors[J].Environmental Science&Technology,2013,51(9):5108.
[4] GU J,XU G J,LIU Y. An integrated AMBBR and IFAS-SBR process for municipal wastewater treatment towards enhanced energy recovery, reduced energy consumption and sludge production[J]. Water Research,2017,110:262-269.
[5] ZINATIZADEH A A L,GHAYTOOLI E. Simultaneous nitrogen and carbon removal from wastewater at different operating conditions in a moving bed biofilm reactor(MBBR):process modeling and optimization[J]. Journal of the Taiwan Institute of Chemical Engineers,2015,53:98-111.
[6] CASAS M E,CHHETRI R K,OOI G,et al. Biodegradation of pharmaceuticals in hospital wastewater by staged moving bed biofilm Reactors(MBBR)[J]. Water Research, 2015,83:293-302.
[7]李亮,张志鹏,印显东. MBR在污水处理与回用工艺中的应用[J].环境与发展,2018,30(7):59.LI L,ZHANG Z P,YIN X D. Application of MBR in wastewater treatment and reuse process[J]. Environment and Development,2018,30(7):59.
[8] E20环境产业研究院.中国MBR市场调研分析报告(2014版)[R].北京:E20环境产业研究院,2014.
[9]叶杰旭,穆永杰,孙德智,等. MBBR-MBR组合工艺处理生活垃圾沥滤液的研究[J].工业水处理,2013,33(1):20-24.YE J X,MU Y J,SUN D Z,et al. Treatment of leachate from a domestic waste incineration plant by MBBR-MBR combined process[J]. Industrial Water Treatment,2013,33(1):20-24.
[10] RODRIGUEZ-SANCHEZ A, CARLOSLEYVA-DIAZ J,GONZALEZ-LOPEZ J,et al. Membrane bioreactor and hybrid moving bed biofilm reactor-membrane bioreactor for the treatment of variable salinity wastewater:influence of biomass concentration and hydraulic retention time[J]. Chemical Engineering Journal,2018,336:102-111.
[11] LEYVA-DIAZ J C,POYATOS J M. Start-up of membrane bioreactor and hybrid moving bed biofilm reactor-membrane bioreactor:kinetic study[J]. Water Sciene and Technology,2015,72(11):1948-53.
[12] DENG L J,GUO W S,NGO H H,et al. New functional biocarriers for enhancing the performance of a hybrid moving bed biofilm reactor-membrane bioreactor system[J]. Bioresource Technology,2016,208:87-93.
[13]张恒亮,段亮,姚美辰,等. MBBR-MBR组合工艺处理生活污水效能及膜污染研究[J].环境工程技术学报,2019,9(3)245-251.ZHANG H L,DUAN L,YAO M C,et al. Study on performance and membrane fouling of MBBR-MBR combined process for treatment of domestic wastewater[J]. Journal of Environmental Engineering Technology,2019,9(3):245-251.
[14] PINTO H B,DE SOUZA B M,DEZOTTI M. Treatment of a pesticide industry wastewater mixture in a moving bed biofilm reactor followed by conventional and membrane processes for water reuse[J]. Journal of Cleaner Production,2018,201:1061-1070.
[15] SAIDI A,MASMOUDI K,NOLDE E,et al. Organic matter degradation in a greywater recycling system using a multistage moving bed biofilm reactor(MBBR)[J]. Water Science&Technology,2017,76(11/12):3328-3339.
[16] ZHENG W L,WEN X H,ZHANG B,et al. Selective effect and elimination of antibiotics in membrane bioreactor of urban wastewater treatment plant[J]. Science of the Total Environment,2019,646:1293-1303.
[17] DUAN L,JIANG W,SONG Y H,et al. The characteristics of extracellular polymeric substances and soluble microbial products in moving bed biofilm reactor-membrane bioreactor[J].Bioresource Technology,2013,148:436-442.
[18]王丹丹,罗清威.移动床-膜生物法餐饮废水深度处理[J].环境工程,2015,33(12):19-23.WANG D D,LUO Q W. Depth treatment of restaurant wastewater by moving bed biofilm-membrane process[J]. Environmental Engineering,2015,33(12):19-23.
[19]温宇惠,林子源,王佳乐,等.温度对ASBBR处理榨菜废水及微生物种群的影响[J].中国给水排水,2018,34(9):20-25.WEN Y H,LIN Z Y,WANG J L,et al. Effect of temperature on performance and microbial population of pilotscale ASBBR treating hypersaline mustard tuber wastewater[J]. China Water&Wastewater,2018,34(9):20-25.
[20]田园,段亮,宋永会,等.不同污泥龄膜生物反应器内微生物的群落结构特征[J].环境科学研究,2015,28(3):453-459.TIAN Y,DUAN L,SONG Y H,et al. Characteristics of microbial community structure in membrane reactors under different solid retention times[J]. Research of Environmental Sciences,2015,28(3):453-459.
[21] ZHU Y,ZHANG Y,REN H Q,et al. Physicochemical characteristics and microbial community evolution of biofilms during the start-up period in a moving bedbiofilm reactor[J].Bioresource Technology,2015,180:345-351
[22]毛跃建.废水处理系统中重要功能类群Thauera属种群结构与功能的研究[D].上海:上海交通大学,2009.
[23] ZILLES J L,PECCIA J,KIM M W,et al. Involvement of rhodocyclus-related organisms in phosphorus removal in full-scale wastewater treatment plants[J]. Applied and Environmental Microbiology,2002,68(6):2763-2769.
[24]连丽丽,姜华,朱昌雄.约氏不动杆菌(Acinetobacter johnsonii)的分离及其聚磷特性的研究[J].辽宁农业科学,2009(2):18-21.
[25] van der WAARDE J,KROONEMAN J,GEURKINK B,et al.Molecular monitoring of bulking sludge in industrial wastewater treatment plants[J]. Water Science&Technology,2002,46(1/2):551-558.
[26]王萍,余志晟,齐嵘,等.丝状细菌污泥膨胀的FISH探针研究进展[J].应用与环境生物学报,2012,18(4):705-712.WANG P,YU Z S,QI R,et al. Progress on FISH probes of sludge bulking caused by filamentous bacteria[J]. Chinese Journal of Applied&Environmental Biology,2012,18(4):705-712.
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