三叶木通叶斑病防治药剂的室内筛选
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摘要
针对导致三叶木通叶斑病的病原菌细极链格孢,选择不同成分的药剂,通过生长速率法和孢子萌发法检测各药剂对该真菌的抑制率,筛选防治三叶木通叶斑病的适用药剂。不同药剂抑制率测定表明:戊唑醇对该病原菌菌丝生长的抑制力最强,多抗霉素的抑制力最弱;各种药剂对该菌孢子萌发抑制能力的大小依次为:戊唑醇、代森锰锌、异菌脲、百菌清、多抗霉素、农抗120。实验结果表明,戊唑醇、代森锰锌、异菌脲可以作为三叶木通叶斑病的防治药剂。
Different ingredients of pharmaceuticals have been choosen to screen fungicides which can prevent and treat Akebia trifoliate leaf spot by grouth rate and spore germination assay. The inhibition test of agents on the mycelial grouth rate showed that tebuconazole among the screened agents is best, while polyoxins is weakest. The inhibition test of spores of pathogen showed that the ability to inhibit spore germination of the drugs in the order is tebuconazole,mancozeb,iprodione,chlorothalonil,Polyoxin,pyrimidine nucleoside antibacterial agent. Experiments showed that Tebuconazole, mancozeb and iprodione can be choosen as fungicides to prevent and control disease occurrence.
Different ingredients of pharmaceuticals have been choosen to screen fungicides which can prevent and treat Akebia trifoliate leaf spot by grouth rate and spore germination assay. The inhibition test of agents on the mycelial grouth rate showed that tebuconazole among the screened agents is best, while polyoxins is weakest. The inhibition test of spores of pathogen showed that the ability to inhibit spore germination of the drugs in the order is tebuconazole,mancozeb,iprodione,chlorothalonil,Polyoxin,pyrimidine nucleoside antibacterial agent. Experiments showed that Tebuconazole, mancozeb and iprodione can be choosen as fungicides to prevent and control disease occurrence.
引文
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[10] 张丹华,张红楠,吴頔,等.重庆地区枇杷叶斑病病原菌初步鉴定及防治药剂室内筛选[J].中国南方果树,2017,46(4):102-106.
[11] 郑肖兰,赵爽,韩小雯,等.海南省南繁区玉米链格孢叶斑病病原菌鉴定及其生物学特性[J].江苏农业科学,2018,46(6):82-87.
[12] 张贺,秦斌华,胡高旗,等.杧果链格孢叶斑病菌的生物学特性研究[J].中国南方果树,2018,47(3):73-76.
[13] 张铮,李秀青,刘昱锋.三叶木通叶斑病病原菌的生物学特性及生物防治[J].陕西师范大学学报(自然科学版),2015,43(5):61-66.
[2] 国家药典委员会编.中华人民共和国药典(一部)[M].北京:化学工业出版社,2015:63-64,297.
[3] YOSHIHIRO M,MINPEI K,AKIHITO Y,et al.Triterpenes and triterpene saponins from the stems of Akebia trifoliate[J].Chemical and Pharmaceutical Bulletin,2003,51(8):960-965.
[4] ELIZABETH A M,KARAM F A S.In vitro screening of tumoricidal properties of international medicinal herbs:part Ⅱ[J].Phytotherapy Research,2010,24(12):1813-1824.
[5] 梁宝方,郭海彪,袁欣,等.常春藤皂苷元抗抑郁药效评价[J].中国药理学与毒理学杂志,2012,26(3):447-448.
[6] 梁志怀,魏林,彭俊彩,等.人工栽培三叶木通褐斑病病原菌鉴定及防治药剂室内筛选[J].植物保护,2009,35(4):158-161.
[7] 刘昱锋,张铮,马银峰.三叶木通叶斑病病原菌鉴定[J].植物保护,2013,39(2):57-62.
[8] 刘娟.柑桔园链格孢菌的鉴定与防治[D].重庆:西南大学,2011.
[9] 张佳星,李玲,戴德江,等.白术枯斑病原鉴定、生物学特性及其对不同药剂的敏感性研究[J].植物病理学报,2018,48(5):682-692.
[10] 张丹华,张红楠,吴頔,等.重庆地区枇杷叶斑病病原菌初步鉴定及防治药剂室内筛选[J].中国南方果树,2017,46(4):102-106.
[11] 郑肖兰,赵爽,韩小雯,等.海南省南繁区玉米链格孢叶斑病病原菌鉴定及其生物学特性[J].江苏农业科学,2018,46(6):82-87.
[12] 张贺,秦斌华,胡高旗,等.杧果链格孢叶斑病菌的生物学特性研究[J].中国南方果树,2018,47(3):73-76.
[13] 张铮,李秀青,刘昱锋.三叶木通叶斑病病原菌的生物学特性及生物防治[J].陕西师范大学学报(自然科学版),2015,43(5):61-66.