某猪场猪瘟典型病例综合诊断及病毒E2基因分析
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摘要
2018年7月,山东省某育肥猪场猪群突然发病并出现大量死亡,病猪临床表现高热、皮肤潮红并布满点状出血。为查明发病原因,对病猪进行病理剖检并采样,经实验室综合检测,初步诊断为猪瘟病毒感染。对检测到的CSFV E2基因进行了分子测序及核酸序列同源性对比,最终确诊为2.1d亚型猪瘟病毒感染。结果显示,本实验室分离到的CSFV(命名为SDXT2018)与21株参考毒株的E2基因核苷酸序列同源性为81.3%～96.2%。与21株参考毒株中同源性最高的是2.1d亚型的JQ001834,同源性达96.2%;与2.3亚型的经典毒株HQ148061的同源性最低,为81.3%。结果说明,我国猪瘟病毒流行毒株的变异趋势越发严峻,以致于常规疫苗免疫的猪场有可能爆发典型猪瘟疫情,这对养猪业构成了严重威胁。因此,亟待人们开展病毒遗传变异趋势的研究,并针对病毒抗原特性研制、生产高效疫苗。
In July 2018, a deathful disease outbroke in a fattening pigs farm in Shandong province. Clinical symptoms included high fever, flushed skin with dotted bleeding. It was preliminarily diagnosed as Classical Swine Fever(CSF) after autopsy and a comprehensive laboratory detection. Then, the E2 gene of Classical Swine Fever virus(CSFV) was detected and sequenced. This outbreak was confirmed as infection of CSFV. The nucleotide sequence homology between the strain in this study(named SDXT2018) and reference strains was from 81.3% to96.2%. In reference strains, SDXT2018 was closest to JQ001834 that belongings to 2.1 d subtype. The homology between SDXT2018 and AF091507, which was classical attenuated hog fever vaccine strain, was just 81.3%. The results showed variation of the epidemic strains of CSFV in China is becoming severe, which seriously threat the swine industry. Classical Swine Fever could outbreak in immunity-failure farms and cause enormous economic loss. It is urgent to research the trend of viral variation and produce new efficient vaccine.
In July 2018, a deathful disease outbroke in a fattening pigs farm in Shandong province. Clinical symptoms included high fever, flushed skin with dotted bleeding. It was preliminarily diagnosed as Classical Swine Fever(CSF) after autopsy and a comprehensive laboratory detection. Then, the E2 gene of Classical Swine Fever virus(CSFV) was detected and sequenced. This outbreak was confirmed as infection of CSFV. The nucleotide sequence homology between the strain in this study(named SDXT2018) and reference strains was from 81.3% to96.2%. In reference strains, SDXT2018 was closest to JQ001834 that belongings to 2.1 d subtype. The homology between SDXT2018 and AF091507, which was classical attenuated hog fever vaccine strain, was just 81.3%. The results showed variation of the epidemic strains of CSFV in China is becoming severe, which seriously threat the swine industry. Classical Swine Fever could outbreak in immunity-failure farms and cause enormous economic loss. It is urgent to research the trend of viral variation and produce new efficient vaccine.
引文
1 Tong C,Chen N,Liao X,Yuan X,Sun M,Li X,et al.Continuous passaging of a recombinant C-Strain virus in PK-15 cells selects culture-adapted variants that showed enhanced replication but failed to induce fever in rabbits.J Microbiol Biotechnol 2017;27(9):1701-10.
2 Blome S,Staubach C,Henke J,Carlson J,Beer M.Classical swine fever-an updated review.Viruses 2017;9(4):pii:E86.
3段辉.山东省某猪场猪瘟病例的诊断及病毒分离鉴定(硕士论文).山东农业大学(Duan Hui.Comprehensive diagnosis,separation and identification of Classical Swine Fever virus in a pig farm of Shandong province.Shanedong Agricultural University),2016.
4 Guo X,Sang S,Guo J,Jian A,Duan Q,Ji J,et al.A magnetoelastic biosensor based on E2 glycoprotein for wireless detection of Classical Swine Fever Virus E2 antibody.Sci Rep 2017;7(1):15626.
5张瑞琴.山西猪瘟抗体水平调查及野毒株E2遗传变异多样性分析(硕士论文).吉林大学(Zhang Ruiqin.Survey of the average of CSF antibody in Shanxi and diversity of the E2 wild strain genetic variation.Ji Lin University),2014.
6马梓承,古金元,彭涛,王玉超,刘照虎,孟凡亮,等.一例高致死性急性猪丹毒病例的综合诊断与防治.中国动物检疫(Ma Zicheng,Gu Jinyuan,Peng Tao,Wang Yuchao,Liu Zhaohu,Meng Fanliang,et al.Comprehensive diagnosis and control of a case of High Fatal Acute Swine Erysipelas.China Animal Health Inspection)2018;35(4):96-100.
7王清华,任炜杰,包静月,戈胜强,李金明,李林,等.我国首例非洲猪瘟的确诊.中国动物检疫(Wangle Qinghua,Ren Weijie,Bao Jingyue,Ge Shengqiang,Li Jingming,Li Lin,et al.The frist outbreak of African Swine Fever was confirmed in China.China Animal Health Inspection)2018;35(9):1-4.
8林祥梅,韩雪清,王景林.外来动物疫病.北京:科学出版社,2014,258-69.
9 Vlasova A,Grebennikova T,Zaberezhny A,Greiser-Wilke I,Floegel-Niesmann G,Kurinnov V,et al.Molecular epidemiology of Classical Swine Fever in the russian federation.J Vet Med B Infect Dis Vet Public Health 2003;50(8):363-7.
10林荣泉.非洲猪瘟与猪瘟的鉴定及防控技术.肉类工业(Lin Rongquan.The prevention and control technology of the African Swine Fever virus and the Classical Swine Fever virus Identification.Meat Industry)2001(3):25-8.
11王琴.猪瘟病毒流行病学、病原致病特性及猪瘟综合防制研究.中国农业科技导报(Wang Qing.Epidemiology,characterization of pathogenicity of Classical Swine Fever virus and control strategies of Classical Swine Fever.Journal of Agricultural Science and Technology)2006:8(5):13-8.
12 Hu D,LüL,Gu J,Chen T,Xiao Y,Liu S.Genetic diversity and positive selection analysis of Classical Swine Fever virus envelope protein gene E2 in eastchina under C-Strain vaccination.Front Microbiol 2016;7:85.
2 Blome S,Staubach C,Henke J,Carlson J,Beer M.Classical swine fever-an updated review.Viruses 2017;9(4):pii:E86.
3段辉.山东省某猪场猪瘟病例的诊断及病毒分离鉴定(硕士论文).山东农业大学(Duan Hui.Comprehensive diagnosis,separation and identification of Classical Swine Fever virus in a pig farm of Shandong province.Shanedong Agricultural University),2016.
4 Guo X,Sang S,Guo J,Jian A,Duan Q,Ji J,et al.A magnetoelastic biosensor based on E2 glycoprotein for wireless detection of Classical Swine Fever Virus E2 antibody.Sci Rep 2017;7(1):15626.
5张瑞琴.山西猪瘟抗体水平调查及野毒株E2遗传变异多样性分析(硕士论文).吉林大学(Zhang Ruiqin.Survey of the average of CSF antibody in Shanxi and diversity of the E2 wild strain genetic variation.Ji Lin University),2014.
6马梓承,古金元,彭涛,王玉超,刘照虎,孟凡亮,等.一例高致死性急性猪丹毒病例的综合诊断与防治.中国动物检疫(Ma Zicheng,Gu Jinyuan,Peng Tao,Wang Yuchao,Liu Zhaohu,Meng Fanliang,et al.Comprehensive diagnosis and control of a case of High Fatal Acute Swine Erysipelas.China Animal Health Inspection)2018;35(4):96-100.
7王清华,任炜杰,包静月,戈胜强,李金明,李林,等.我国首例非洲猪瘟的确诊.中国动物检疫(Wangle Qinghua,Ren Weijie,Bao Jingyue,Ge Shengqiang,Li Jingming,Li Lin,et al.The frist outbreak of African Swine Fever was confirmed in China.China Animal Health Inspection)2018;35(9):1-4.
8林祥梅,韩雪清,王景林.外来动物疫病.北京:科学出版社,2014,258-69.
9 Vlasova A,Grebennikova T,Zaberezhny A,Greiser-Wilke I,Floegel-Niesmann G,Kurinnov V,et al.Molecular epidemiology of Classical Swine Fever in the russian federation.J Vet Med B Infect Dis Vet Public Health 2003;50(8):363-7.
10林荣泉.非洲猪瘟与猪瘟的鉴定及防控技术.肉类工业(Lin Rongquan.The prevention and control technology of the African Swine Fever virus and the Classical Swine Fever virus Identification.Meat Industry)2001(3):25-8.
11王琴.猪瘟病毒流行病学、病原致病特性及猪瘟综合防制研究.中国农业科技导报(Wang Qing.Epidemiology,characterization of pathogenicity of Classical Swine Fever virus and control strategies of Classical Swine Fever.Journal of Agricultural Science and Technology)2006:8(5):13-8.
12 Hu D,LüL,Gu J,Chen T,Xiao Y,Liu S.Genetic diversity and positive selection analysis of Classical Swine Fever virus envelope protein gene E2 in eastchina under C-Strain vaccination.Front Microbiol 2016;7:85.