摘要
目的:建立HPLC法同时测定给喜古讷-6中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量。方法:采用高效液相色谱法Welchrom C18色谱柱(4.6mm×300mm,5μm),以甲醇-0.1%磷酸水溶液(68∶32)为流动相,流速1mL·min-1,检测波长254nm,柱温40℃。结果:5种成分在各自范围内均呈良好的线性关系(r≥0.9991);平均加样回收率95.73%~102.91%,RSD 1.3%~1.6%。结论:高效液相色谱法灵敏度高,该实验方法简便快捷、重现性好,为完善蒙药给喜古讷-6汤的质量控制提供依据。
Objective:HPLC method was established for simultaneous determination of Geixigune-6,Aloeemodin,Rodin,Emodin,Chrysophanol,Physcion. Method:High performance liquid chromatography was performed by Welchrom C18 column(4.6 mm×300 mm,5μm)The aqueous solution of methanol 0.1% phosphoric acid(68∶32) was used as the mobile phase,The flow rate was 1 mL·min-1.The detction wavelength was 254 nm,and the column temperature was 30℃. Result:The five components showed good linear relationship in their respective range(r ≥0.9991). The average recovery was 95.73% ~102.91% RSD 1.3% ~1.6.Conclusion:High performance liquid chromatography has high sensitivity,this experimental method is simple and fast,with good reproducibility,accurate and reliable results,providing a scientific basis for improving the quality control standard of Mongolian medicine for Geixigune-6 decoction.
引文
[1]内蒙古自治区卫生厅.内蒙古蒙成药标准[S].赤峰:内蒙古科学技术出版社,1984:140.
[2]巴根那.蒙医方剂学[M].呼和浩特:内蒙古人民出版社,2007:277.
[3]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:23.
[4]毕云生,范云飞,王英萍,等.HPLC法同时测定消氮颗粒中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚五种成分的含量[J].放军药学学报,2014,4(30):324-326.
[5]李思远,滕亮,詹羽姣,等.沼生忍冬藤化学成分及3种成分的含量测定[J].中国实验方剂学杂志,2017,23(19):94-98.
[6]呼海涛,刘雅琳,田双双,等.鄂西香茶菜中4个二萜类成分的含量及其对细胞增殖的影响[J].中国实验方剂学杂志,2017,21(23):59-63.
[7]李锐.HPLC同时测定大黄利胆胶囊中5种蒽醌类成分的总含量[J].食品与药品,2017,19(5):351-353.
[8]乔立业,方李,曹燕丽,等.高效液相色谱法测定新保肾片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量[J].药学实践杂志,2015,33(5):438-440.
[9]朱振红,赵晓峰,纪兆芬,等.HPLC法测定蒙药敖龙斯乐吉胶囊中大黄素的含量[J].中国民族医药杂志,2008,14(1):47-48.
[10]谷静逸,尹海波,赵容,等.辽宁省5种老鹳草中5种活性成分的含量测定[J].中国实验方剂学杂志,2016,22(6):26-29.
[11]白凤,董玉,李斌鑫,等.HPLC法同时测定蒙药复方协日嘎-4中5种有效成分的含量[J].中国药房,2018,29(14):1964-1967.
[12]张海涛,吕颖.高效液相色谱法测定蒙药制剂色布如-9中胡椒碱的含量[J].中国民族医药杂志,2017,6:43-44.
[13]郑毓珍,卢静华,孙玉琦.HPLC法同时测定白梅花中6种黄酮类成分[J].中成药,2018,40(9):2007-2010.