应用体外仿生消化法对华贵栉孔扇贝体内镉进行生物可给率研究
|
摘要
镉(Cd)是可食用贝类体内的主要污染物之一,为了更科学有效地研究贝类Cd的食用风险,以对Cd生物富集能力强的浅海贝类——华贵栉孔扇贝(Chlamys nobills)为研究对象,采用模拟胃肠道物理化学环境的体外仿生消化法,测定扇贝体内Cd的生物可给率。结果表明:华贵栉孔扇贝体内Cd的可给率范围在36.5%～63.3%之间,均值47.9%,消化食糜中Cd的主要形态为MT-Cd和GSH-Cd。扇贝各组织Cd的生物可给率大小依次为性腺(51.2%)、闭壳肌(44.9%)、内脏(40.6%)、鳃瓣/外套膜(37.4%),性腺质量分数与可给率呈显著负相关(R=-0.840,P<0.05),内脏质量分数则与可给率呈极显著正相关(R=0.996,P<0.01)。鳃瓣/外套膜和闭壳肌是扇贝消化食糜中Cd含量的主要来源(占63.5%),内脏对消化食糜中Cd的贡献率则高于其相应的质量分数,食用前若去除内脏,可以减少约25%Cd的膳食摄入量。
Cadmium was one of the easiest accumulated environmental pollutants,and Chlamys nobills which belonged to the kind of shellfish in shallow sea and had significant bio-accumulation ability for Cd,was taken as an example to study the dietary intake risk scientifically and effectively.And then the method of in vitro digestion,which could simulate the physical and chemical environment of the gastrointestinal tract,was used to obtain the bioaccessibility of Cd in C.nobills for dietary intake.The result showed that the range of Cd bioaccessibility in C.nobills was from 36.5 to 63.3%(mean to 47.9%)and the main forms of Cd in chyme were MT-Cd and GSH-Cd.The Cd bioaccessibility of different tissues was in the order as sex gland(51.2%),adductor muscle(44.9%),viscera(40.6%)and gill lamella/mantle(37.4%).It also seemed that there was significant negative correlation between the mass fraction of sex gland and its bioaccessibility(R=-0.840,P<0.05),while a extremely significant positive correlation could be found between the mass fraction of visceral and its bioaccessibility(R=0.996,P<0.01).And the content of Cd in chyme mainly came from the tissue of gill lamella/mantle and adductor muscle(up to 63.5%).Because of the higher Cd contribution of viscera in the chyme than its corresponding mass fraction,the dietary intake risk of Cd might be reduced by a quarter if this tissue was removed before eating.
Cadmium was one of the easiest accumulated environmental pollutants,and Chlamys nobills which belonged to the kind of shellfish in shallow sea and had significant bio-accumulation ability for Cd,was taken as an example to study the dietary intake risk scientifically and effectively.And then the method of in vitro digestion,which could simulate the physical and chemical environment of the gastrointestinal tract,was used to obtain the bioaccessibility of Cd in C.nobills for dietary intake.The result showed that the range of Cd bioaccessibility in C.nobills was from 36.5 to 63.3%(mean to 47.9%)and the main forms of Cd in chyme were MT-Cd and GSH-Cd.The Cd bioaccessibility of different tissues was in the order as sex gland(51.2%),adductor muscle(44.9%),viscera(40.6%)and gill lamella/mantle(37.4%).It also seemed that there was significant negative correlation between the mass fraction of sex gland and its bioaccessibility(R=-0.840,P<0.05),while a extremely significant positive correlation could be found between the mass fraction of visceral and its bioaccessibility(R=0.996,P<0.01).And the content of Cd in chyme mainly came from the tissue of gill lamella/mantle and adductor muscle(up to 63.5%).Because of the higher Cd contribution of viscera in the chyme than its corresponding mass fraction,the dietary intake risk of Cd might be reduced by a quarter if this tissue was removed before eating.
引文
[1]张翠，翟毓秀，宁劲松，等．Cd在水生动物体内的研究概况[J]．水产科学．2007,29(8):465-470．
[2]王丽娟，吴成业．水产品中Cd的形态分析及其危害[J]．福建水产，2014,36(1):78-84．
[3]李凤琴，徐娇，刘飒娜．生物利用率在食品污染物风险评估中的应用[J]．中国食品卫生杂志，2011,23(1):17-22．
[4]兰砥中，雷鸣，周爽，等．体外模拟实验法评价湘南某矿区大米中重金属的人体健康风险[J]．农业环境科学学报，2014,33(10):1897-1903．
[5]He M,Ke C H,Wang W X.Effects of cooking and subcellular distribution on the bioaccessibility of trace elements in two marine fish species[J].Journal of Agricultural&Food Chemistry,2010,58(6):3517-3523．
[6]Laparra J M,Vélez D,Montoro R,et al.Estimation of arsenic bioaccessibility in edible seaweed by in vitro digestion method[J].Journal of Agricultural&Food Chemistry,2003,51(20):6080-6085．
[7]赵艳芳，尚德荣，宁劲松，等．体积排阻高效液相色谱-电感耦合等离子体质谱法测定紫菜中镉(Cd)的形态[J]．水产学报，2012,36(1):148-152．
[8]赵艳芳，尚德荣，宁劲松，等．体积排阻高效液相色谱-电感耦合等离子体质谱法测定海产贝类中Cd的形态[J]．分析化学，2012,40(5):681-686．
[9]洪雄业．兴化湾养殖贝类重金属累积及健康风险评价[J]．海峡科学，2014,(8):7-9,27．
[10]蔡艳，周亦君，吴晓艺，等．3种海洋贝类重金属污染及食用风险评价研究[J]．核农学报，2016,30(6):1126-1134．
[11]王增焕，王许诺，林钦，等．体外仿生法研究牡蛎和蛤仔体内微量金属元素的生物可给性[J]．分析化学，2016,44(2):247-251．
[12]Oomen A G,Rompelberg C J,Bruil M A,et al.Development of an in Vitro digestion model for estimating the bioaccessibility of soil contaminants[J].Archives of Environmental Contamination and Toxicolog,2003,44(3),281-287．
[13]杨妙峰．体外仿生消化在华贵栉孔扇贝(Chlamys nobilis)体内Cd的生物可给性中的应用[J]．渔业科学进展，2016,37(6):94-99．
[14]赵艳芳，宁劲松，翟毓秀，等．栉孔扇贝不同组织中Cd的微区分布特征及其变化规律[J]．水产学报，2016,40(8):1203-1210．
[15]刘发义，吴玉霖．重金属污染物在海洋生物体内的积累和解毒机理[J]．海洋科学，1988,12(5):63-66．
[16]Choi H J,Ji J,Chung K H,et al.Cadmium bioaccumulation and detoxification in the gill and digestive gland of the Antarctrc bivalve Laternula elliptica[J].Comparative Biochemistry&Physiology Part C Toxicology&Pharmacology,2007,145(2):227-235．
[17]Binz P A,Kgi J H R.Metallothionein:Molecular evolution and classification[M]//Klaassen C.MetallothioneinⅣ．Switzerland:Birkhuser Verlag Basel,1999:7-13．
[18]励建荣，宣伟，李学鹏，等．金属硫蛋白的研究进展[J]．食品科学，2010,31(17):392-396．
[19]马荣萱，任乃林，齐广才．半胱氨酸与Cd(Ⅱ)的配合物极谱波研究[J]．延安大学学报(自然科学版)，1998,17(3):64-66．
[20]张玉秀，柴团耀，G·rard Burkard．植物耐重金属机理研究进展[J]．植物学报，1999,41(5):453-457．
[21]黄纪念，宋国辉，孙强，等．脱脂麦胚中谷胱甘肽与麦胚蛋白的提取[J]．食品科学，2010,31(22):139-144．
[22]岳新萍，周杰昊，程时．锌7与Cd7-金属硫蛋白清除羟自由基的比较[J]．生物化学与生物物理进展，1996,23(4):352-355．
[23]马元庆，张秀珍，孙玉增，等．栉孔扇贝对重金属的富集效应研究[J]．水产学报，2010,34(10):1572-1578.
[2]王丽娟，吴成业．水产品中Cd的形态分析及其危害[J]．福建水产，2014,36(1):78-84．
[3]李凤琴，徐娇，刘飒娜．生物利用率在食品污染物风险评估中的应用[J]．中国食品卫生杂志，2011,23(1):17-22．
[4]兰砥中，雷鸣，周爽，等．体外模拟实验法评价湘南某矿区大米中重金属的人体健康风险[J]．农业环境科学学报，2014,33(10):1897-1903．
[5]He M,Ke C H,Wang W X.Effects of cooking and subcellular distribution on the bioaccessibility of trace elements in two marine fish species[J].Journal of Agricultural&Food Chemistry,2010,58(6):3517-3523．
[6]Laparra J M,Vélez D,Montoro R,et al.Estimation of arsenic bioaccessibility in edible seaweed by in vitro digestion method[J].Journal of Agricultural&Food Chemistry,2003,51(20):6080-6085．
[7]赵艳芳，尚德荣，宁劲松，等．体积排阻高效液相色谱-电感耦合等离子体质谱法测定紫菜中镉(Cd)的形态[J]．水产学报，2012,36(1):148-152．
[8]赵艳芳，尚德荣，宁劲松，等．体积排阻高效液相色谱-电感耦合等离子体质谱法测定海产贝类中Cd的形态[J]．分析化学，2012,40(5):681-686．
[9]洪雄业．兴化湾养殖贝类重金属累积及健康风险评价[J]．海峡科学，2014,(8):7-9,27．
[10]蔡艳，周亦君，吴晓艺，等．3种海洋贝类重金属污染及食用风险评价研究[J]．核农学报，2016,30(6):1126-1134．
[11]王增焕，王许诺，林钦，等．体外仿生法研究牡蛎和蛤仔体内微量金属元素的生物可给性[J]．分析化学，2016,44(2):247-251．
[12]Oomen A G,Rompelberg C J,Bruil M A,et al.Development of an in Vitro digestion model for estimating the bioaccessibility of soil contaminants[J].Archives of Environmental Contamination and Toxicolog,2003,44(3),281-287．
[13]杨妙峰．体外仿生消化在华贵栉孔扇贝(Chlamys nobilis)体内Cd的生物可给性中的应用[J]．渔业科学进展，2016,37(6):94-99．
[14]赵艳芳，宁劲松，翟毓秀，等．栉孔扇贝不同组织中Cd的微区分布特征及其变化规律[J]．水产学报，2016,40(8):1203-1210．
[15]刘发义，吴玉霖．重金属污染物在海洋生物体内的积累和解毒机理[J]．海洋科学，1988,12(5):63-66．
[16]Choi H J,Ji J,Chung K H,et al.Cadmium bioaccumulation and detoxification in the gill and digestive gland of the Antarctrc bivalve Laternula elliptica[J].Comparative Biochemistry&Physiology Part C Toxicology&Pharmacology,2007,145(2):227-235．
[17]Binz P A,Kgi J H R.Metallothionein:Molecular evolution and classification[M]//Klaassen C.MetallothioneinⅣ．Switzerland:Birkhuser Verlag Basel,1999:7-13．
[18]励建荣，宣伟，李学鹏，等．金属硫蛋白的研究进展[J]．食品科学，2010,31(17):392-396．
[19]马荣萱，任乃林，齐广才．半胱氨酸与Cd(Ⅱ)的配合物极谱波研究[J]．延安大学学报(自然科学版)，1998,17(3):64-66．
[20]张玉秀，柴团耀，G·rard Burkard．植物耐重金属机理研究进展[J]．植物学报，1999,41(5):453-457．
[21]黄纪念，宋国辉，孙强，等．脱脂麦胚中谷胱甘肽与麦胚蛋白的提取[J]．食品科学，2010,31(22):139-144．
[22]岳新萍，周杰昊，程时．锌7与Cd7-金属硫蛋白清除羟自由基的比较[J]．生物化学与生物物理进展，1996,23(4):352-355．
[23]马元庆，张秀珍，孙玉增，等．栉孔扇贝对重金属的富集效应研究[J]．水产学报，2010,34(10):1572-1578.