8个籼型恢复系相关农艺性状的配合力分析
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摘要
研究杂交组合的遗传特性,挖掘优异的种质资源对于杂种优势利用具有重要意义。本试验以8个恢复系为父本、21个不育系为母本,按照不完全双列杂交8×21(NCII)设计配制168个组合,对8个农艺性状的配合力及遗传参数进行分析。结果表明:有效穗数、穗长、千粒重主要受基因加性效应影响,株高、实粒数、结实率主要受基因非加性效应互作影响;杂交组合的株高、有效穗数、穗长、千粒重、单株产量的表现主要依赖于母本,总粒数受父本影响更大,实粒数、结实率取决于双亲的表现;有效穗数、单株产量在后代遗传中的稳定性较差,易受环境和基因非加性效应影响;843A、宜香1A、沪旱7A、昌恢871、昌恢T025、雅占为配合力好的亲本,宜香1A、昌恢T025具有最好的一般配合力(GCA)效应值;宜香1A/昌香恢1号、千乡059A/昌恢121、广8A/昌恢881为较优组合,宜香1A/昌香恢1号特殊配合力(SCA)最优。对恢复系主要农艺性状进行配合力分析,为杂交水稻恢复系的选育提供了一定的科学依据。
In order to make use of the heterosis in hybridization breeding,it is of great significance to explore excellent germplasm resources and analyze the combining ability. By deployment of 8 restorer lines and 21 sterile lines,which served as male and female parent,respectively,this study generated 168 hybrids following the incomplete diallel hybridization 8×21(NCII). These materials were analyzed for the combining ability and the genetic parameters of 8 agronomic traits. The results showed that the effective panicle number,panicle length and 1000-grain weight were mainly affected by the additive effect,and the plant height,filled grain number and the seed setting rate were mainly affected by the interaction of non-additive effect. The plant height,effective panicle number,spike length,1000-grain weight and yield per plant of hybrid combination were largely femaledependent,while the total grain number was largely male-dependent. The grain number and seed setting rate were genetically contributed by both parents. The effective panicle number and yield per plant were depending on the interaction between genetic components and environment conditions. The parents with combining ability were 843 A,Yixiang 1 A,Huhan7 A,Changhui 871,Changhui T025 and Yazhan. Out of these genotypes,Yixiang 1 A and Changhui T025 exhibited the best General Combining Ability(GCA)effect value. The hybrid combination Yixiang 1 A/Changxianghui 1 exhibited the excellence on Special Combining Ability(SCA),and the hybrids including Yixiang 1 A/Changxianghui 1,Qianxiang 059 A/Changhui 121,Guang8 A/Changhui 881 were qualified. Thus,by investigating the combining ability of main agronomic characters in restorer lines,this study provided some scientific basis for the breeding of hybrid rice restorer lines.
In order to make use of the heterosis in hybridization breeding,it is of great significance to explore excellent germplasm resources and analyze the combining ability. By deployment of 8 restorer lines and 21 sterile lines,which served as male and female parent,respectively,this study generated 168 hybrids following the incomplete diallel hybridization 8×21(NCII). These materials were analyzed for the combining ability and the genetic parameters of 8 agronomic traits. The results showed that the effective panicle number,panicle length and 1000-grain weight were mainly affected by the additive effect,and the plant height,filled grain number and the seed setting rate were mainly affected by the interaction of non-additive effect. The plant height,effective panicle number,spike length,1000-grain weight and yield per plant of hybrid combination were largely femaledependent,while the total grain number was largely male-dependent. The grain number and seed setting rate were genetically contributed by both parents. The effective panicle number and yield per plant were depending on the interaction between genetic components and environment conditions. The parents with combining ability were 843 A,Yixiang 1 A,Huhan7 A,Changhui 871,Changhui T025 and Yazhan. Out of these genotypes,Yixiang 1 A and Changhui T025 exhibited the best General Combining Ability(GCA)effect value. The hybrid combination Yixiang 1 A/Changxianghui 1 exhibited the excellence on Special Combining Ability(SCA),and the hybrids including Yixiang 1 A/Changxianghui 1,Qianxiang 059 A/Changhui 121,Guang8 A/Changhui 881 were qualified. Thus,by investigating the combining ability of main agronomic characters in restorer lines,this study provided some scientific basis for the breeding of hybrid rice restorer lines.
引文
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[8]张书芹，韩雪松，胡恺宁.植物杂种优势遗传基础研究进展.分子植物育种，2017,15(11):4734-4740Zhang S Q,Han X S,Hu K N. Research progress on genetic basis of heterosis in plants. Molecular Plant Breeding,2017,15(11):4734-4740
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[11]王绍林，李振宇，王志兴，陈广红，夏明，郑英杰.北方粳型两系杂交稻产量性状的配合力分析.北方水稻，2005(5):3-6Wang S L,Li Z Y,Wallg Z X,Chen G H,Xia M,Zheng Y J.The analysis of combining ability on yield characters of northern two-lines cross-japonica-rice. Reclaim and Rice Cultivation,2005(5):3-6
[12]余传元，江玲，肖应辉，翟虎渠，万建民.籼型染色体置换片段在杂交粳稻中的配合力分析.作物学报，2008,26(8):1308-1316Yu C Y Jiang L,Xiao Y H,Zhai H Q,Wan J M. Combining ability of yield-component traits for indica chromosome substituted segments in japonica hybrids. Acta Gronomica Sinica,2008,26(8):1308-1316
[13]王和寿，陈健勇.若干杂交稻亲本的配合力分析.亚热带农业研究，2008,4(1):1-5Wang H S,Chen J Y. Analysis of the combining ability of several rice varieties. Subtropical Agriculture Research,2008,4(1):1-5
[14]游年顺，雷捷成.杂交稻数量性状配合力分析.福建稻麦科技，1994(2):16-19You N S,Lei J C. Analysis of combining ability of quantitative characters in hybrid rice. Fujian Science and Technology of Rice and Wheat,1994(2):16-19
[15]包灵丰，林纲，赵德明，李云武，曾健，贺兵.宜恢1577的籼粳特性鉴别和产量配合力分析.植物遗传资源学报，2006,7(2):231-233B a o L F,L i n G,Z h a o D M,L i Y W,Z e n g J,H e B.Identification of indical-japonical character and analysis on the yield combination ability for the new germplasm Yihui1577.Journal of Plant Genetic Resources,2006,7(5):231-233
[16]刘龙钦，江文清，梁康迳，周仕全，谢冬容，邱慧明，饶溶晖，马妙明，彭吕清.若干籼型杂交稻亲本的农艺性状配合力分析.福建稻麦科技，2018,36(1):1-6Liu L Q,Jiang W Q,Liang K J,Zhou S Q,Xie D R,Qiu H M,Rao R H,Ma M M,Peng L Q. Combining ability analysis of agronomic traits of several indica hybrid rice parents. Fujian Science and Technology of Rice and Wheat,2018,36(1):1-6
[17]宋宇，邹小云，贺浩华，傅军如，李海波，徐亮，辛晓云.籼型三系杂交水稻产量及其相关性状的配合力分析.江西农业大学学报，2004,26(5):719-725Song Y,Zou X Y,He H H,Fu J R,Li H B,Xu L,Xin X Y.Analysis on combining ability of yield characters and related characters in three-line indica hybrid rice. Acta Agriculturae Universitis Jiangxiensis,2004,26(5):719-725
[18]李云，钟珺，肖林长，席建才，谢建萍，连垚，周卫营，黎为兵.杂交水稻亲本产量相关性状的配合力分析.安徽农业科学，2018,46(11):24-26,87Li Y,Zhong J,Xiao L C,Xi J C,Xie J P,Lian Y,Zhou W Y,Li W B. Combining ability analysis of yield-related traits on parents of hybrid rice. Journal of Anhui Agricultural Sciences,2018,46(11):24-26,87
[19]游书梅，曹应江，郑家奎，蒋开锋，张涛，杨莉，杨乾华，万先齐，郭小蛟，秦俭，罗婧，李昭祥，高磊.中国与南亚水稻恢复系资源产量性状的配合力分析.植物遗传资源学报，2016,17(5):861-865You S M,Cao Y J,Zheng J K,Jiang K F,Zhang T,Yang L,Yang Q H,Wan X Q,Guo X J,Qin J,Luo J,Li Z X,Gao L.Analysis on combining ability of yield traits between China and South Asia in rice restorer lines. Journal of Plant Genetic Resources,2016,17(5):861-865
[20]欧阳林娟，孙玥，彭东华，王春雷，周大虎，傅军如，贺晓鹏，贺浩华，彭小松. 5个新育成转Bt基因抗虫恢复系配合力分析.江西农业大学学报，2018,40(4):663-670Ouyang L J,Sun Y,Peng D H,Wang C L,Zhou D H,Fu J R,He X P,He H H,Peng X S. An analysis of the combining ability of five new bred trans-Bt gene restorer lines with pest resistance. Acta Agriculturae Universitis Jiangxiensis,2018,40(4):663-670
[21]李志华，穆婷婷，刘鑫，李会霞，田岗. 4个谷子不育系主要农艺性状的配合力分析.作物杂志，2018(3):61-67Li Z H,Mu T T,Liu X,Li H X,Tian G. Combining ability analysis of main agronomic characters of four millet sterile lines. Crops,2018(3):61-67
[22]蒋钰东，况浩池，罗俊涛，何兴材，杨扬，付均，郑军，陈光珍，曾正明.三系杂交稻优质不育系德66A主要经济性状的配合力分析.中国稻米，2018,24(6):118-121Jiang Y D,Kuang H C,Luo J T,He X C,Yang Y,Fu J,Zheng J,Chen G Z,Zeng Z M. Analysis on combining ability of main economic characters of three-line sterile line De66A. China Rice,2018,24(6):118-121
[2]朱英国.杂交水稻研究50年.科学通报，2016,61(35):18-25Zhu Y G. Fifty years of hybrid rice research in China. Chinese Science Bulletin,2016,61(35):18-25
[3] Shull G H. The composition of a field of maize. Journal of Heredity,1908,os-4(1):296-301
[4] Hua J P,Xing Y Z,Wu W R,Xu C G,Sun X L,Yu S B,Zhang Q F. Single-locus heterotic effects and dominance by dominance interactions can adequately explain the genetic basis of heterosis in an elite rice hybrid. Proceedings of the National Academy of Sciences of the United States of America,2003,100(5):2574-2579
[5] Shen G J,Zhan W,Chen H X,Xing Y Z. Dominance and epistasis are the main contributors to heterosis for plant height in rice. Plant Science,2014,215-216:11-18
[6] Luo X J,Fu Y C,Zhang P J,Wu S,Tian F,Liu J Y,Zhu Z F,Yang J S,Sun C Q. Additive and over-dominant effects resulting from epistatic loci are the primary genetic basis of heterosis in rice. Journal of Integrative Plant Biology,2009,51(4):393-408
[7] Dan Z W,Hu J,Zhou W,Yao G X,Zhu R S,Huang W C,Zhu Y G. Hierarchical additive effects on heterosis in rice(Oryza sativa L.). Frontiers in Plant Science,2015,6:738
[8]张书芹，韩雪松，胡恺宁.植物杂种优势遗传基础研究进展.分子植物育种，2017,15(11):4734-4740Zhang S Q,Han X S,Hu K N. Research progress on genetic basis of heterosis in plants. Molecular Plant Breeding,2017,15(11):4734-4740
[9] Nadali B,Babaeian J N. Heterosis and combining ability analysis for yield and related-yield traits in hybrid rice.International Journal of Biology,2010,2(2):222
[10]黄远樟，刘来福.作物数量遗传学基础——六、配合力：不完全双列杂交.遗传，1980,2(2):45-48Huang Y Z,Liu L F. Crop quantitative genetics—Six,Combining ability:Incomplete diallel hybridization. Hereditas,1980,2(2):45-48
[11]王绍林，李振宇，王志兴，陈广红，夏明，郑英杰.北方粳型两系杂交稻产量性状的配合力分析.北方水稻，2005(5):3-6Wang S L,Li Z Y,Wallg Z X,Chen G H,Xia M,Zheng Y J.The analysis of combining ability on yield characters of northern two-lines cross-japonica-rice. Reclaim and Rice Cultivation,2005(5):3-6
[12]余传元，江玲，肖应辉，翟虎渠，万建民.籼型染色体置换片段在杂交粳稻中的配合力分析.作物学报，2008,26(8):1308-1316Yu C Y Jiang L,Xiao Y H,Zhai H Q,Wan J M. Combining ability of yield-component traits for indica chromosome substituted segments in japonica hybrids. Acta Gronomica Sinica,2008,26(8):1308-1316
[13]王和寿，陈健勇.若干杂交稻亲本的配合力分析.亚热带农业研究，2008,4(1):1-5Wang H S,Chen J Y. Analysis of the combining ability of several rice varieties. Subtropical Agriculture Research,2008,4(1):1-5
[14]游年顺，雷捷成.杂交稻数量性状配合力分析.福建稻麦科技，1994(2):16-19You N S,Lei J C. Analysis of combining ability of quantitative characters in hybrid rice. Fujian Science and Technology of Rice and Wheat,1994(2):16-19
[15]包灵丰，林纲，赵德明，李云武，曾健，贺兵.宜恢1577的籼粳特性鉴别和产量配合力分析.植物遗传资源学报，2006,7(2):231-233B a o L F,L i n G,Z h a o D M,L i Y W,Z e n g J,H e B.Identification of indical-japonical character and analysis on the yield combination ability for the new germplasm Yihui1577.Journal of Plant Genetic Resources,2006,7(5):231-233
[16]刘龙钦，江文清，梁康迳，周仕全，谢冬容，邱慧明，饶溶晖，马妙明，彭吕清.若干籼型杂交稻亲本的农艺性状配合力分析.福建稻麦科技，2018,36(1):1-6Liu L Q,Jiang W Q,Liang K J,Zhou S Q,Xie D R,Qiu H M,Rao R H,Ma M M,Peng L Q. Combining ability analysis of agronomic traits of several indica hybrid rice parents. Fujian Science and Technology of Rice and Wheat,2018,36(1):1-6
[17]宋宇，邹小云，贺浩华，傅军如，李海波，徐亮，辛晓云.籼型三系杂交水稻产量及其相关性状的配合力分析.江西农业大学学报，2004,26(5):719-725Song Y,Zou X Y,He H H,Fu J R,Li H B,Xu L,Xin X Y.Analysis on combining ability of yield characters and related characters in three-line indica hybrid rice. Acta Agriculturae Universitis Jiangxiensis,2004,26(5):719-725
[18]李云，钟珺，肖林长，席建才，谢建萍，连垚，周卫营，黎为兵.杂交水稻亲本产量相关性状的配合力分析.安徽农业科学，2018,46(11):24-26,87Li Y,Zhong J,Xiao L C,Xi J C,Xie J P,Lian Y,Zhou W Y,Li W B. Combining ability analysis of yield-related traits on parents of hybrid rice. Journal of Anhui Agricultural Sciences,2018,46(11):24-26,87
[19]游书梅，曹应江，郑家奎，蒋开锋，张涛，杨莉，杨乾华，万先齐，郭小蛟，秦俭，罗婧，李昭祥，高磊.中国与南亚水稻恢复系资源产量性状的配合力分析.植物遗传资源学报，2016,17(5):861-865You S M,Cao Y J,Zheng J K,Jiang K F,Zhang T,Yang L,Yang Q H,Wan X Q,Guo X J,Qin J,Luo J,Li Z X,Gao L.Analysis on combining ability of yield traits between China and South Asia in rice restorer lines. Journal of Plant Genetic Resources,2016,17(5):861-865
[20]欧阳林娟，孙玥，彭东华，王春雷，周大虎，傅军如，贺晓鹏，贺浩华，彭小松. 5个新育成转Bt基因抗虫恢复系配合力分析.江西农业大学学报，2018,40(4):663-670Ouyang L J,Sun Y,Peng D H,Wang C L,Zhou D H,Fu J R,He X P,He H H,Peng X S. An analysis of the combining ability of five new bred trans-Bt gene restorer lines with pest resistance. Acta Agriculturae Universitis Jiangxiensis,2018,40(4):663-670
[21]李志华，穆婷婷，刘鑫，李会霞，田岗. 4个谷子不育系主要农艺性状的配合力分析.作物杂志，2018(3):61-67Li Z H,Mu T T,Liu X,Li H X,Tian G. Combining ability analysis of main agronomic characters of four millet sterile lines. Crops,2018(3):61-67
[22]蒋钰东，况浩池，罗俊涛，何兴材，杨扬，付均，郑军，陈光珍，曾正明.三系杂交稻优质不育系德66A主要经济性状的配合力分析.中国稻米，2018,24(6):118-121Jiang Y D,Kuang H C,Luo J T,He X C,Yang Y,Fu J,Zheng J,Chen G Z,Zeng Z M. Analysis on combining ability of main economic characters of three-line sterile line De66A. China Rice,2018,24(6):118-121