水稻矮缩病毒对黑尾叶蝉生长发育和繁殖的影响
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摘要
【目的】水稻普通矮缩病是一种常见的病毒性病害,对水稻生产造成了严重的威胁。水稻矮缩病毒(Rice dwarf virus, RDV)是该病害的病原物,主要依赖黑尾叶蝉经卵以持久增殖方式传播。探明水稻矮缩病毒对介体昆虫黑尾叶蝉的影响,可为黑尾叶蝉的种群治理和水稻普通矮缩病的可持续控制提供依据。【方法】设置健康与感病2个水稻处理,在室内27℃条件下,观测黑尾叶蝉的生长发育、种群增长情况,组建实验种群生命表。【结果】介体昆虫黑尾叶蝉在感染水稻矮缩病毒的水稻上取食后,若虫期存活率、成虫鲜重无显著差异,雌成虫寿命和产卵量略有延长/增加,但雌雄虫若虫发育历期和雄成虫寿命显著缩短,种群数量显著增多,5个生命表参数中净生殖率显著提高。【结论】感染水稻矮缩病毒的水稻对介体黑尾叶蝉存在有利的间接影响,水稻矮缩病毒有利于黑尾叶蝉种群的增长。
【Objective】Rice dwarf disease is a kind of common viral disease, which causes serious threats to rice production. Rice dwarf virus(RDV) is the pathogeny and the spread of the disease is mainly rely on Nephotettix cincticeps(Uhler) in a persistent-propagative manner. The exploration on the effects of RDV on the vector insect green rice leafhopper can provide basis for the population control of N. cincticeps and the sustainable control of rice dwarf disease.【Method】Two treatments(non-infected and RDV-infected) were set at 27℃ in laboratory, the growth and population increase of N.cincticeps were observed and the life tables of experimental populations were constructed.【Result】The results showed that the nymph survival, fresh body weight per male/female adult of N.cincticeps fed on RDV-infected rice plants did not significantly differ from those on healthy plants, longevity and fecundity of female adult were slightly longer/higher, and the population number of adults was significantly increased, whereas the growth period of male/female nymph and longevity of male adult was decreased obviously. Also, the net reproductive rate(R0) significantly increased among the five life table parameters.【Conclusion】RDV-infected rice plants has positive indirect effects on N.cincticeps and RDV is beneficial to thepopulation increase of N.cincticeps.
【Objective】Rice dwarf disease is a kind of common viral disease, which causes serious threats to rice production. Rice dwarf virus(RDV) is the pathogeny and the spread of the disease is mainly rely on Nephotettix cincticeps(Uhler) in a persistent-propagative manner. The exploration on the effects of RDV on the vector insect green rice leafhopper can provide basis for the population control of N. cincticeps and the sustainable control of rice dwarf disease.【Method】Two treatments(non-infected and RDV-infected) were set at 27℃ in laboratory, the growth and population increase of N.cincticeps were observed and the life tables of experimental populations were constructed.【Result】The results showed that the nymph survival, fresh body weight per male/female adult of N.cincticeps fed on RDV-infected rice plants did not significantly differ from those on healthy plants, longevity and fecundity of female adult were slightly longer/higher, and the population number of adults was significantly increased, whereas the growth period of male/female nymph and longevity of male adult was decreased obviously. Also, the net reproductive rate(R0) significantly increased among the five life table parameters.【Conclusion】RDV-infected rice plants has positive indirect effects on N.cincticeps and RDV is beneficial to thepopulation increase of N.cincticeps.
引文
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[4]FUJITA D,DOI K,YOSHIMURA A,YASUI H.Molecular mapping of a novel gene,Grh5,conferring resistance to green rice leafhopper(Nephotettix cincticeps Uhler)in rice,Oryza sativa L[J].Theoretical and Applied Genetics,2006,113(4):567-573.doi:10.1007/s00122-006-0270-x.
[5]周霞，程家安，胡阳，娄永根．转Bt基因水稻克螟稻对黑尾叶蝉种群增长的影响[J]．中国水稻科学，2005,19(1):74-78.doi:10.3321/j.issn:1001-7216.2005.01.014.ZHOU X,CHENG J A,HU Y,LOU Y G.Effects of transgenic Bt rice on the population development of Nephotettix cincticeps[J].Chinese Journal of Rice Science,2005,19(1):74-78.doi:10.3321/j.issn:1001-7216.2005.01.014.
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[7]叶健，龚雨晴，方荣祥．病毒-昆虫-植物三者互作研究进展及展望[J]．中国科学院院刊（专题：作物病虫害的导向性防控），2017,32(8):845-855.doi:CNKI:SUN:KYYX.0.2017-08-006.YE J,GONG Y Q,FANG R X.Research progress and perspective of tripartite interaction of virus-vector-plant in vector-borne viral diseases[J].China Academic journal(Decoding the Mechanisms of Bio-interactions for Targeted Management of Agricultural Pests),2017,32(8):845-855.doi:CNKI:SUN:KYYX.0.2017-08-006.
[8]关桂静，赵恒燕，王洪苏，刘金香．病毒-植物互作对介体昆虫生物学特性的影响[J]．生物技术通报，2017,33(4):44-50.doi:10.13560/j.cnki.biotech.bull.1985.2017.04.006.GUAN G J,ZHAO H Y,WANG H S,LIU J X.Effects of virus-plant interaction on biological characteristics of insects as vectors[J].Biotechnology Bulletin,2017,33(4):44-50.doi:10.13560/j.cnki.biotech.bull.1985.2017.04.006.
[9]SCHLIEPHAKE E,HABEKUSS A,SCHOLZ M,ORDON F.Barley yellow dwarf virus transmission and feeding behavior of rhopalosiphum padi on hordeum bulbosum clones[J].Entomologia Experimentalis Et Applicata,2013,146(3):347-356.doi:10.1111/eea.12033.
[10]LI S,WANG S J,WANG X,LI X L,ZI J Y,GE S S,CHENG Z B,ZHOU T,JI Y H,DENG J H,WONG S M,ZHOU Y J.Rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos[J].Scientific Reports,2015,5(5):417-446.doi:10.1038/srep07883.
[11]STOUT M J,THALER J S,THOMMA B P H J.Plant-mediated interactions between pathogenic microorganisms and herbivorous arthropods[J].Annual Review of Entomology,2006,51:663-689.doi:10.1146/annurev.ento.51.110104.151117.
[12]NAKASUJI F,KIRITANI K.Ill effects of rice dwarf virus upon its vector,Nephotettix circticeps Uhler(Hemiptera:Deltocephalidae),and its significance for changes in relative abundance of infected individuals among vector populations[J].Applied Entomology and Zoology,1970,5(1):1-12.doi:10.1303/aez.5.1.
[13]梁莉，郭建洋，田俊策，陈洋，胡萃，叶恭银．水稻矮缩病毒对黑尾叶蝉卵巢发育与产卵量的影响[J]．植物保护学报，2010,37(4):375-376.LIANG L,GUO J Y,TIAN J C,CHEN Y,HU C,YE G Y.Effects of rice dwarf virus on ovarian development and fecundity of the green leaf hopper,Nephotettix cincticeps(Fabricius)[J].Acta Phytophylacica Sinica,2010,37(4):375-376.
[14]WANG Q J,HAN N S,DANG C,LU Z B,WANG F,YAO H W,PENG YF,STANLEY D,Ye G Y.Combined influence of Bt rice and rice dwarf virus on biological parameters of a non-target herbivore,Nephotettix cincticeps(Uhler)(Hemiptera:Cicadellidae)[J].PLoS ONE,2017,12(7):e0181258.doi:10.1371/journal.pone.0181258.
[15]王前进，党聪，方琦，叶恭银．水稻矮缩病毒对介体昆虫黑尾叶蝉生物学参数及种群增长的影响[J]．中国水稻科学，2018,32(1):89-95.doi:10.16819/j.1001-7216.2018.7090.WANG Q J,DANG C,FANG Q,YE G Y.Influence of rice dwarf virus on biological parameters and population growth of vector insect Nephotettix cincticeps(Uhler)(Hemiptera:Cicadellidae)[J].Chinese Journal of Rice Science,2018,32(1):89-95.doi:10.16819/j.1001-7216.2018.7090.
[16]HIBINO H.Biology and epidemiology of rice viruses[J].Annual Review of Phytopathology,1996,34(4):249-274.doi:10.1146/annurev.phyto.34.1.249.
[17]YOSH I DA S,FOMO D A,COCK J H.L a borator y manua l for physiological studies of rice[M].The International Rice Research Institute,Manila,Philippines,1976:61-66.
[18]SPSS.SPSS for Windows User’s Guide Release 16[R].SPSS Inc,Chicago,2007.
[19]MAIA A H,LUIZ A J,CAMPANHOLA C.Statistical inference on associated fertility life table parameters using jackknife technique:computational aspects[J].Journal of Economic Entomology,2000,93(2):511-518.doi:10.1603/0022-0493-93.2.511.
[20]李毅，周雪平，韩成贵，范在丰，吴袓建，王锡锋．稻麦主要病毒病识别与控制[M]．北京：中国农业出版社，2011:15-26.L I Y,ZHOU X P,H A N C G,FA N Z F,WU Z J,WA NG X F.Identification and control of main viruses of rice and wheat[M].Beijing:China Agriculture Press,2011:15-26.
[21]BLUA M J,PERRING T M,MADORE M A.Plant virus-induced changes in aphid population development and temporal fluctuations in plant nutrients[J].Journal of Chemical Ecology,1994,20(3):691-707.doi:10.1007/BF02059607.
[22]COLVIN J,OMONGO C A,GOVINDAPPA M R,ST EVENSONP C,GOWDA M M N,GIBSON G,SUSAN E S,MUNIYAPPA V.Host-plant viral infection effects on arthropod-vector population growth,development and behavior:management and epidemiological implications[J].Advances in Virus Research,2006,67(6):419-452.doi:10.1016/S0065-3527(06)67011-5.
[23]FIEBIG M,POEHLING H M,BORGEMEISTER C.Barley yellow dwarf virus,wheat,and Sitobion avenae:a case of trilateral interactions[J].Entomologia Experimentalis et Applicata,2004,110(1):11-21.doi:10.1111/j.0013-8703.2004.00115.x.
[24]LUAN J B,YAO D M,ZHANG T,WALLING L L,YANG M,WANGY J,LIU S S.Suppression of terpenoid synthesis in plants by a virus promotes its mutualism with vectors[J].Ecology Letters,2012,16:390-398.doi:10.1111/ele.12055.
[25]LI R,WELDEGERGIS B T,LI J,JUNG C,QU J,SUN Y W,QIANH M,TEE C,VAN LOON J J,DICKE M,CHUA N H,LIU S S,YE J.Virulence factors of geminivirus interact with MYC 2 to subvert plant resistance and promote vector performance[J].The Plant Cell,2014,26:4991-5008.doi:10.1105/tpc.114.133181.
[26]ALAZEM M,LIN N S.Roles of plant hormones in the regulation of host-virus interactions[J].Molecular Plant Pathology,2015,16(5):529-540.doi:10.1111/mpp.12204.
[2]谢联辉，林奇英，魏太云，吴祖建．水稻病毒[M]．北京：科学出版社，2016:158-182.XIE L H,LIN Q Y,WEI T Y,WU Z J.Rice Viruses[M].Beijing:Science Press,2016:158-182.
[3]谢联辉，林奇英．我国水稻病毒病研究的进展[J]．中国农业科学，1984,17(6):58-65.XIE L H,LIN Q Y.Advances in the studies of rice virus disease in China[J].Scientia Agricultura Sinica,1984,17(6):58-65.
[4]FUJITA D,DOI K,YOSHIMURA A,YASUI H.Molecular mapping of a novel gene,Grh5,conferring resistance to green rice leafhopper(Nephotettix cincticeps Uhler)in rice,Oryza sativa L[J].Theoretical and Applied Genetics,2006,113(4):567-573.doi:10.1007/s00122-006-0270-x.
[5]周霞，程家安，胡阳，娄永根．转Bt基因水稻克螟稻对黑尾叶蝉种群增长的影响[J]．中国水稻科学，2005,19(1):74-78.doi:10.3321/j.issn:1001-7216.2005.01.014.ZHOU X,CHENG J A,HU Y,LOU Y G.Effects of transgenic Bt rice on the population development of Nephotettix cincticeps[J].Chinese Journal of Rice Science,2005,19(1):74-78.doi:10.3321/j.issn:1001-7216.2005.01.014.
[6]OMURA T,YAN J.Role of outer capsid proteins in transmission of phytoreovirus by insect vectors[J].Advances in Virus Research,1999,54(1):15-43.doi:10.1016/S0065-3527(08)60364-4.
[7]叶健，龚雨晴，方荣祥．病毒-昆虫-植物三者互作研究进展及展望[J]．中国科学院院刊（专题：作物病虫害的导向性防控），2017,32(8):845-855.doi:CNKI:SUN:KYYX.0.2017-08-006.YE J,GONG Y Q,FANG R X.Research progress and perspective of tripartite interaction of virus-vector-plant in vector-borne viral diseases[J].China Academic journal(Decoding the Mechanisms of Bio-interactions for Targeted Management of Agricultural Pests),2017,32(8):845-855.doi:CNKI:SUN:KYYX.0.2017-08-006.
[8]关桂静，赵恒燕，王洪苏，刘金香．病毒-植物互作对介体昆虫生物学特性的影响[J]．生物技术通报，2017,33(4):44-50.doi:10.13560/j.cnki.biotech.bull.1985.2017.04.006.GUAN G J,ZHAO H Y,WANG H S,LIU J X.Effects of virus-plant interaction on biological characteristics of insects as vectors[J].Biotechnology Bulletin,2017,33(4):44-50.doi:10.13560/j.cnki.biotech.bull.1985.2017.04.006.
[9]SCHLIEPHAKE E,HABEKUSS A,SCHOLZ M,ORDON F.Barley yellow dwarf virus transmission and feeding behavior of rhopalosiphum padi on hordeum bulbosum clones[J].Entomologia Experimentalis Et Applicata,2013,146(3):347-356.doi:10.1111/eea.12033.
[10]LI S,WANG S J,WANG X,LI X L,ZI J Y,GE S S,CHENG Z B,ZHOU T,JI Y H,DENG J H,WONG S M,ZHOU Y J.Rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos[J].Scientific Reports,2015,5(5):417-446.doi:10.1038/srep07883.
[11]STOUT M J,THALER J S,THOMMA B P H J.Plant-mediated interactions between pathogenic microorganisms and herbivorous arthropods[J].Annual Review of Entomology,2006,51:663-689.doi:10.1146/annurev.ento.51.110104.151117.
[12]NAKASUJI F,KIRITANI K.Ill effects of rice dwarf virus upon its vector,Nephotettix circticeps Uhler(Hemiptera:Deltocephalidae),and its significance for changes in relative abundance of infected individuals among vector populations[J].Applied Entomology and Zoology,1970,5(1):1-12.doi:10.1303/aez.5.1.
[13]梁莉，郭建洋，田俊策，陈洋，胡萃，叶恭银．水稻矮缩病毒对黑尾叶蝉卵巢发育与产卵量的影响[J]．植物保护学报，2010,37(4):375-376.LIANG L,GUO J Y,TIAN J C,CHEN Y,HU C,YE G Y.Effects of rice dwarf virus on ovarian development and fecundity of the green leaf hopper,Nephotettix cincticeps(Fabricius)[J].Acta Phytophylacica Sinica,2010,37(4):375-376.
[14]WANG Q J,HAN N S,DANG C,LU Z B,WANG F,YAO H W,PENG YF,STANLEY D,Ye G Y.Combined influence of Bt rice and rice dwarf virus on biological parameters of a non-target herbivore,Nephotettix cincticeps(Uhler)(Hemiptera:Cicadellidae)[J].PLoS ONE,2017,12(7):e0181258.doi:10.1371/journal.pone.0181258.
[15]王前进，党聪，方琦，叶恭银．水稻矮缩病毒对介体昆虫黑尾叶蝉生物学参数及种群增长的影响[J]．中国水稻科学，2018,32(1):89-95.doi:10.16819/j.1001-7216.2018.7090.WANG Q J,DANG C,FANG Q,YE G Y.Influence of rice dwarf virus on biological parameters and population growth of vector insect Nephotettix cincticeps(Uhler)(Hemiptera:Cicadellidae)[J].Chinese Journal of Rice Science,2018,32(1):89-95.doi:10.16819/j.1001-7216.2018.7090.
[16]HIBINO H.Biology and epidemiology of rice viruses[J].Annual Review of Phytopathology,1996,34(4):249-274.doi:10.1146/annurev.phyto.34.1.249.
[17]YOSH I DA S,FOMO D A,COCK J H.L a borator y manua l for physiological studies of rice[M].The International Rice Research Institute,Manila,Philippines,1976:61-66.
[18]SPSS.SPSS for Windows User’s Guide Release 16[R].SPSS Inc,Chicago,2007.
[19]MAIA A H,LUIZ A J,CAMPANHOLA C.Statistical inference on associated fertility life table parameters using jackknife technique:computational aspects[J].Journal of Economic Entomology,2000,93(2):511-518.doi:10.1603/0022-0493-93.2.511.
[20]李毅，周雪平，韩成贵，范在丰，吴袓建，王锡锋．稻麦主要病毒病识别与控制[M]．北京：中国农业出版社，2011:15-26.L I Y,ZHOU X P,H A N C G,FA N Z F,WU Z J,WA NG X F.Identification and control of main viruses of rice and wheat[M].Beijing:China Agriculture Press,2011:15-26.
[21]BLUA M J,PERRING T M,MADORE M A.Plant virus-induced changes in aphid population development and temporal fluctuations in plant nutrients[J].Journal of Chemical Ecology,1994,20(3):691-707.doi:10.1007/BF02059607.
[22]COLVIN J,OMONGO C A,GOVINDAPPA M R,ST EVENSONP C,GOWDA M M N,GIBSON G,SUSAN E S,MUNIYAPPA V.Host-plant viral infection effects on arthropod-vector population growth,development and behavior:management and epidemiological implications[J].Advances in Virus Research,2006,67(6):419-452.doi:10.1016/S0065-3527(06)67011-5.
[23]FIEBIG M,POEHLING H M,BORGEMEISTER C.Barley yellow dwarf virus,wheat,and Sitobion avenae:a case of trilateral interactions[J].Entomologia Experimentalis et Applicata,2004,110(1):11-21.doi:10.1111/j.0013-8703.2004.00115.x.
[24]LUAN J B,YAO D M,ZHANG T,WALLING L L,YANG M,WANGY J,LIU S S.Suppression of terpenoid synthesis in plants by a virus promotes its mutualism with vectors[J].Ecology Letters,2012,16:390-398.doi:10.1111/ele.12055.
[25]LI R,WELDEGERGIS B T,LI J,JUNG C,QU J,SUN Y W,QIANH M,TEE C,VAN LOON J J,DICKE M,CHUA N H,LIU S S,YE J.Virulence factors of geminivirus interact with MYC 2 to subvert plant resistance and promote vector performance[J].The Plant Cell,2014,26:4991-5008.doi:10.1105/tpc.114.133181.
[26]ALAZEM M,LIN N S.Roles of plant hormones in the regulation of host-virus interactions[J].Molecular Plant Pathology,2015,16(5):529-540.doi:10.1111/mpp.12204.