生物炭对洗脱堆放的多环芳烃土壤中微生物群落结构与功能的影响研究
摘要
本研究采用人工湿地植物(水葱)烧制的秸秆生物炭作为微生物增效试剂,投加到经1:5(kg/L)土水比加入浓度为0. 4g/L阴离子表面活性剂十二烷基苯磺酸钠(SDBS)洗脱堆放土壤中,每隔1～2周翻动土壤,并保持土壤含水率25%～35%,30天后堆放土壤经过高通量测序发现细菌菌群生物量总数增加,不饱和脂肪酸比例增大,1-hydroxy-2-naphthoate dioxygenase和PAH dioxygenase large subunit等基因(酶)的相对丰度增加,生物炭可大大大改善洗脱土壤的微生物群落生境。
引文
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[2]Sun M.,Luo Y.,Teng Y.,Jia Z.,etc..Remediation of polycyclic aromatic hydrocarbon and metal-contaminated soil by successive methyl-β-cyclodextrin-enhanced soil washing-microbial augmentation:a laboratory evaluation[J].Environmental Science and Pollution Research,2013,20(2):976~986
[3]Yu H.,Zhu L.,Zhou W..Enhanced desorption and biodegradation of phenanthrene in soil-water systems with the presence of anionic-nonionic mixed surfactants[J].Journal of Hazardous Materials,2007,142(1-2):354~361
[4]Zheng G.,Selvam A.,Wong J..Enhanced Solubilization and Desorption of Organochlorine Pesticides(OCPs)from Soil by Oil-Swollen Micelles Formed with a Nonionic Surfactant[J].Environmental Science&Technology,2012,46(21):12062~12068
[5]Li F.,Zhu L.;Wang L.,Zhan Y..Gene expression of an arthrobacter in surfactant-enhanced biodegradation of a hydrophobic organic compound[J].Environmental Science&Technology,2015,49(6):3698~3704
[6]张栋.表面活性剂对PAHs微生物界面行为的影响及调控机制[D].杭州:浙江大学,2013