不同接种物对木薯乙醇废液高温厌氧发酵的影响
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摘要
作者采用了4种不同的厌氧污泥作为木薯乙醇废液处理的接种污泥,在高温55℃条件下进行批式实验,并评价了4种不同接种污泥高温厌氧处理木薯乙醇废液的发酵特性。结果表明:高温絮状污泥组、中温絮状污泥组、中温复配污泥组对SCOD的降解率分别为72.1%、72.2%和47.5%,沼气产率分别为188.8、182、109 mL/gTCODadded;而中温颗粒污泥组对SCOD的降解率较低,仅为17.8%,沼气产率为53.7 m L/gTCODadded,这是由于整个发酵过程中VFA浓度较高,抑制了产甲烷菌群对有机物质的转化。中温复配污泥组在第9天时的纤维素酶活性和半纤维素酶活性最大,分别为48.2 U和51.3 U,中温颗粒污泥组酶活力表现较差,在第3天和第5天达到最大值,分别为5.5 U和9.8 U。高温絮状污泥组和中温絮状污泥组的半纤维素酶和纤维素酶活性在第5天时达到最大,分别为10.6、18.7 U和24.6、28.7 U。经过15 d的厌氧发酵,中温复配污泥组粗纤维降解率最大,为41.5%,而其他三种污泥体系粗纤维降解能力一般,其中高温污泥组、中温颗粒污泥组和中温絮状污泥组对粗纤维降解率分别为25.8%、16.1%和29.4%。研究表明中温复配污泥体系对木薯乙醇废液中的固体物质具有较好的降解效果。
The batch experiment was indicated in this study for the cassava alcohol wastewater anaerobic fermentation with the four different inoculations under the temperature of 55 ℃,in order to evaluate the fermentation characteristics of four different inoculation sludge. The results indicated that the degradation rate of SCOD and biogas production rate in the group of thermophilic sludge,mesophilic flocculent sludge and mesophilic mix sludge group was 72.1%,72.2%,47.5%,and188.8,182,109 mL/gTCODadded,respectively. However,the SCOD degradation rate and biogas generation rate in the group of mesophilic granular sludge was only 17.8% and 53.7 mL/gTCODadded,respectively,which due to the inhibited methanogens microorganism by the high concentration of VFA during the fermentation process. The maximum enzyme activity of cellulose and hemicellulase in mesophilic mix sludge group was 48.2 U and 51.3 U on the 9 th day,respectively,while the mesophilic granular sludge group had the lower enzyme activity of 5.5 U on the 3 rd and 9.8 U on the5 th day,respectively. Thermophilic sludge group and mesophilic flocculent sludge had a maximum enzyme activity of 10.6 U,18.7 U and 24.6 U,28.7 U on the 5 th day,respectively. After 15 days of anaerobic fermentation,mesophilic mix sludge group obtained highest crude fiber degradation rate of41.5%,while the thermophilic sludge group,mesophilic mix sludge group and mesophilic flocculent sludge group was 25.8% 16.1% and 29.4%,respectively. The results showed that the mesophilic mix sludge group had better performance in the degradation of the solid material in the cassava alcohol wastewater.
The batch experiment was indicated in this study for the cassava alcohol wastewater anaerobic fermentation with the four different inoculations under the temperature of 55 ℃,in order to evaluate the fermentation characteristics of four different inoculation sludge. The results indicated that the degradation rate of SCOD and biogas production rate in the group of thermophilic sludge,mesophilic flocculent sludge and mesophilic mix sludge group was 72.1%,72.2%,47.5%,and188.8,182,109 mL/gTCODadded,respectively. However,the SCOD degradation rate and biogas generation rate in the group of mesophilic granular sludge was only 17.8% and 53.7 mL/gTCODadded,respectively,which due to the inhibited methanogens microorganism by the high concentration of VFA during the fermentation process. The maximum enzyme activity of cellulose and hemicellulase in mesophilic mix sludge group was 48.2 U and 51.3 U on the 9 th day,respectively,while the mesophilic granular sludge group had the lower enzyme activity of 5.5 U on the 3 rd and 9.8 U on the5 th day,respectively. Thermophilic sludge group and mesophilic flocculent sludge had a maximum enzyme activity of 10.6 U,18.7 U and 24.6 U,28.7 U on the 5 th day,respectively. After 15 days of anaerobic fermentation,mesophilic mix sludge group obtained highest crude fiber degradation rate of41.5%,while the thermophilic sludge group,mesophilic mix sludge group and mesophilic flocculent sludge group was 25.8% 16.1% and 29.4%,respectively. The results showed that the mesophilic mix sludge group had better performance in the degradation of the solid material in the cassava alcohol wastewater.
引文
[1]DAI Shiming,LV Xiwu,LU Huiming.Research on circular economy in the process of alcohol production[J].Industrial Technol ogy&Economy,2006,25(11):126-128.(in Chinese）
[2]BOUALLAGU H,CHEIKH R B,MAROUARI L,et al.Mesophilic biogas production from fruit and vegetable waste in a tubular digester[J].Bioresource Technology,2003,86(1):85-89.
[3]UEMURA S,HARADA H.Treatment of sewage by a UASB reactor under moderate to low temperature conditions[J].Bioresource Technology,2000,72(3):275-282.
[4]SYUTSUBO,NAGAYA,SAKAI,et al.The role of cellulosic bacteria in the anaerobic digestion process[J].China Biogas,2009,5:18-20.
[5]HE Jiang,MAO Zhonggui,ZHANG Qinghua,et al.Construction of a microbial consortium RXS with high degradation ability for cassava residues and studies on its fermentative characteristics[J].Environmental Science,2012,3:1020-1027.(in Chinese）
[6]CHEN Jingrong,XIE li,LUO Gang,et al.Two-stage anaerobic treatment of cassava ethanol wastewater using thermophilic CSTRand mesophilic UASB[J].Industrial Water Treatment,2011,2:33-36.(in Chinese）
[7]WEI Xuemei,ZHANG Yinghui.The discoveries of UASB processing for the treatment of cassava alcohol slops[J].Journal of Xi’an University of Art&Science:Natural Sciences,2011,14(3):59-62.(in Chinese）
[8]BAI Xiaofeng,LI Zifu,WANG Xiaoxi,et al.Biogas production and model for anaerobic fermentation of corn straws by composting treatment[J].Journal of Food Science and Biotechnology,2014,2:151-156.(in Chinese）
[9]VAN S P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.
[10]TAO Zhiping,ZHAO Mingxing,RUAN Wenquan.Effect sodium chloride on biogas generation of kitchen waste by anaerobic digestion[J].Journal of Food Science and Biotechnology,2013,6:596-602.(in Chinese）
[11]ZHOU Chunsheng,YIN Jun.Study on TTC-dehydrogenase activity detection method[J].Journal of Environmental Science,1996,4:400-405.(in Chinese）
[12]高树梅.餐厨垃圾厌氧消化过程中氨氮耐受响应机制研究[D].无锡：江南大学，2015.
[13]YAN Qiusheng,JIANG Chuankui.Method for determination of cellulase activity[J].Genetics,1979,3:33-34.(in Chinese）
[14]国家环保部.水和废水监测分析方法[M].北京：中国环境科学出版社，2002:105-220.
[15]WANG Quan,GONG Changxiu,JIANG Jianguo,et al.Effect of NaCl content on VFA concentration and composition during anaerobic fermentation of kitchen waste[J].China Environmental Science,2014,12:3127-3132.(in Chinese）
[16]ZHANG Jishi,ZHANG Lei,WANG Hua.Study on the city organic waste anaerobic fermentation treatment[J].Ecology and Environment,2005,3:321-324.(in Chinese）
[17]LU Zheng,ZHOU Xingqiu,WU Jiangdong.Study on effect of different initial pH on methane production of biohydrogen fermentative liquid[J].Chinese Journal of Environmental Engineering,2011,5:1115-1118.(in Chinese）
[18]LIU J,WANG W,YANG H,et al.Process of rice straw degradation and dynamic trend of pH by the microbial community MC1[J].Journal of Environmental Sciences-China,2006,18(6):1142-1146.
[19]LI Meiqun,XIONG Xingyao,TAN Xinghe,et al.Effect of temperature on anaerobic biogas fermentation by sweet potato lees[J].Journal of Hunan Agricultural University:Natural Sciences,2010,2:233-23.(in Chinese）
[20]KUMAR R,SINGH S,SINGH O V,et al.Bioconversion of lignocellulosic biomass:biochemical and molecular perspectives[J].Journal of Industrial Microbiology&Biotechnology,2008,35(5):377-391.
[21]TANG Yi,HU Jicui.Study on coenzyme F420 as the anaerobic sludge index[J].China Biogas,1990,1:11-15.(in Chinese）
[22]ZHEN H H,HAN Q Y.Anaerobic digestion of cattail by rumen cultures[J].Waste Management,2005,26(11):1222-1228.
[23]CHANG V S,HOLTZAPPLE M T.Fundamental factors affecting biomass enzymatic reactivity[J].Applied Biochemistry and Biotechnology,2000,84-86(1):5-37.
[24]PARKIN G F,OWEN,W F.Fundamentals of anaerobic digestion of wastewater sludges[J].Journal of Environmental Engineering,1986,112(5):867-920.
[2]BOUALLAGU H,CHEIKH R B,MAROUARI L,et al.Mesophilic biogas production from fruit and vegetable waste in a tubular digester[J].Bioresource Technology,2003,86(1):85-89.
[3]UEMURA S,HARADA H.Treatment of sewage by a UASB reactor under moderate to low temperature conditions[J].Bioresource Technology,2000,72(3):275-282.
[4]SYUTSUBO,NAGAYA,SAKAI,et al.The role of cellulosic bacteria in the anaerobic digestion process[J].China Biogas,2009,5:18-20.
[5]HE Jiang,MAO Zhonggui,ZHANG Qinghua,et al.Construction of a microbial consortium RXS with high degradation ability for cassava residues and studies on its fermentative characteristics[J].Environmental Science,2012,3:1020-1027.(in Chinese）
[6]CHEN Jingrong,XIE li,LUO Gang,et al.Two-stage anaerobic treatment of cassava ethanol wastewater using thermophilic CSTRand mesophilic UASB[J].Industrial Water Treatment,2011,2:33-36.(in Chinese）
[7]WEI Xuemei,ZHANG Yinghui.The discoveries of UASB processing for the treatment of cassava alcohol slops[J].Journal of Xi’an University of Art&Science:Natural Sciences,2011,14(3):59-62.(in Chinese）
[8]BAI Xiaofeng,LI Zifu,WANG Xiaoxi,et al.Biogas production and model for anaerobic fermentation of corn straws by composting treatment[J].Journal of Food Science and Biotechnology,2014,2:151-156.(in Chinese）
[9]VAN S P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.
[10]TAO Zhiping,ZHAO Mingxing,RUAN Wenquan.Effect sodium chloride on biogas generation of kitchen waste by anaerobic digestion[J].Journal of Food Science and Biotechnology,2013,6:596-602.(in Chinese）
[11]ZHOU Chunsheng,YIN Jun.Study on TTC-dehydrogenase activity detection method[J].Journal of Environmental Science,1996,4:400-405.(in Chinese）
[12]高树梅.餐厨垃圾厌氧消化过程中氨氮耐受响应机制研究[D].无锡：江南大学，2015.
[13]YAN Qiusheng,JIANG Chuankui.Method for determination of cellulase activity[J].Genetics,1979,3:33-34.(in Chinese）
[14]国家环保部.水和废水监测分析方法[M].北京：中国环境科学出版社，2002:105-220.
[15]WANG Quan,GONG Changxiu,JIANG Jianguo,et al.Effect of NaCl content on VFA concentration and composition during anaerobic fermentation of kitchen waste[J].China Environmental Science,2014,12:3127-3132.(in Chinese）
[16]ZHANG Jishi,ZHANG Lei,WANG Hua.Study on the city organic waste anaerobic fermentation treatment[J].Ecology and Environment,2005,3:321-324.(in Chinese）
[17]LU Zheng,ZHOU Xingqiu,WU Jiangdong.Study on effect of different initial pH on methane production of biohydrogen fermentative liquid[J].Chinese Journal of Environmental Engineering,2011,5:1115-1118.(in Chinese）
[18]LIU J,WANG W,YANG H,et al.Process of rice straw degradation and dynamic trend of pH by the microbial community MC1[J].Journal of Environmental Sciences-China,2006,18(6):1142-1146.
[19]LI Meiqun,XIONG Xingyao,TAN Xinghe,et al.Effect of temperature on anaerobic biogas fermentation by sweet potato lees[J].Journal of Hunan Agricultural University:Natural Sciences,2010,2:233-23.(in Chinese）
[20]KUMAR R,SINGH S,SINGH O V,et al.Bioconversion of lignocellulosic biomass:biochemical and molecular perspectives[J].Journal of Industrial Microbiology&Biotechnology,2008,35(5):377-391.
[21]TANG Yi,HU Jicui.Study on coenzyme F420 as the anaerobic sludge index[J].China Biogas,1990,1:11-15.(in Chinese）
[22]ZHEN H H,HAN Q Y.Anaerobic digestion of cattail by rumen cultures[J].Waste Management,2005,26(11):1222-1228.
[23]CHANG V S,HOLTZAPPLE M T.Fundamental factors affecting biomass enzymatic reactivity[J].Applied Biochemistry and Biotechnology,2000,84-86(1):5-37.
[24]PARKIN G F,OWEN,W F.Fundamentals of anaerobic digestion of wastewater sludges[J].Journal of Environmental Engineering,1986,112(5):867-920.