猪源产超广谱β-内酰胺酶大肠杆菌的耐药性调查与耐药基因型分析
摘要
为了解猪源产超广谱β-内酰胺酶(Extended Spectrum Beta-Lactamases, ESBLs)大肠杆菌的耐药基因型分布情况,采用CLSI推荐方法进行ESBLs表型检测和确证试验,采用PCR方法 ESBLs耐药基因型分析。结果表明,60.21%(112/186)的猪源大肠杆菌菌株为ESBLs阳性。blaCTX-M、blaTEM、blaSHV和blaOXA型ESBLs菌株检出率分别为91.07%、68.75%、4.46%和1.78%。由此可见,河南部分猪场大肠杆菌广泛流行ESBLs,流行的主要基因型为blaCTX-M型和blaCTX-M+blaTEM型,且多为复合基因型耐药菌株。
引文
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[7]赵凤菊,曹东,李井春,等.猪源大肠埃希菌超广谱β-内酰胺酶的检测及其耐药性分析[J].河南农业科学,2017,46(2):111-115.
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[13]张利勃,刘延庆,乔长涛,等.鸡源性大肠杆菌产超广谱β-内酰胺酶基因型分布[J].中国农学通报,2011,27(20):76-78.
[14]曲志娜,刘红玉,王娟,等.青岛地区产ESBLs鸡源大肠杆菌耐药性调查与优势基因型分析[J].中国农业科学,2015(10):2058-2066.
[15]曹敏,谭艾娟,吕世明,等.贵州部分地区猪源大肠杆菌耐药性分析及ESBLs基因型检测[J].中国畜牧兽医,2016(4):1098-1104.
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[19]Leverstein-van Hall M A,Dierikx C M,Cohen Stuart J,et al.Dutch patients,retail chicken meat and poultry share the same ESBL genes,plasmids and strains[J].Clin Microbiol Infect,2011,17(6):873-880.
[20]Liu W,Chen L,Li H,et al.Novel CTX-Mβ-lactamase genotype distribution and spread into multiple species of Enterobacteriaceae in Changsha,Southern China[J].J Antimicrob Chemother,2009,63:895-900.
[21]Barlow M,Reik R A,Jacobs S D,et al.High rate of mobilization for bla(CTX-M)s[J].Emerg Infect Dis,2008,14(3):423-428.
[22]Shiraki Y,Shibata N,Doi Y,et al.Escherichia coli producing CTX-M-2 beta-lactamase in cattle,Japan[J].Emerg Infect Dis,2004,10(1):69-75.
[23]Meireles D,Leite-Martins L,Bessa L J,et al.Molecular characterization of quinolone resistance mechanisms and extendedspectrum beta-lactamase production in Escherichia coli isolated from dogs[J].Comp Immunol Microbiol Infect Dis,2015,41:43-48.
[24]Carvalho A C,Barbosa A V,Arais L R,et al.Resistance patterns,ESBL genes,and genetic relatedness of Escherichia coli from dogs and owners[J].Braz J Microbiol,2016,47(1):150-158.
[25]宾羽琳,王萌,陈艳,等.食源性产超广谱β-内酰胺酶大肠埃希菌的分布及其同源性研究[J].中国感染控制杂志,2016,15(12):907-912.
[2]Smet A,Martel A,Persoons D,et al.Broad-spectrumβ-lactamases among Enterobacteriaceae of animal origin:molecular aspects,mobility and impact on public health[J].Fems Microbiology Reviews,2010,34(3):295-316.
[3]Jiang H X,LüD H,Chen Z L,et al.High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China[J].Veterinary Journal,2011,187(1):99-103.
[4]Yang H,Chen S,White D G,et al.Characterization of multiple-antimicrobial-resistant Escherichia coli isolates from diseased chickens and swine in China[J].Journal of Clinical Microbiology,2004,42(8):3483-3489.
[5]Zheng H,Zeng Z,Chen S,et al.Prevalence and characterisation of CTX-Mβ-lactamases amongst Escherichia coli isolates from healthy food animals in China[J].International Journal of Antimicrobial Agents,2012,39(4):305-310.
[6]苑丽,刘建华,胡功政,等.鸡大肠杆菌TEM和CTX-M型超广谱β-内酰胺酶基因分型研究[J].中国农业科学,2010,43(20):4310-4316.
[7]赵凤菊,曹东,李井春,等.猪源大肠埃希菌超广谱β-内酰胺酶的检测及其耐药性分析[J].河南农业科学,2017,46(2):111-115.
[8]Canton R,Novais A,Valverde A.Prevalence and spread of extended-spectrumβ-lactamase-producing Enterobacteriaceae in Europe[J].Clin Microbiol Infect,2008,11(1):144-153.
[9]郑新添,张孝辉,吴玲,等.猪源产ESBLs大肠杆菌三种耐药基因检测及分析[J].龙岩学院学报,2015,33(2):100-103.
[10]沈莉萍,徐锋,张维谊,等.1997-2010年上海市猪源大肠杆菌耐药性和产ESBLs菌的基因型检测[J].中国动物检疫,2017(9):26-30,45.
[11]李晴,张泽,曹甸磊,等.养殖废水产ESBLs大肠杆菌多重耐药性及耐药基因检测[J].中国预防兽医学报,2018(6):495-499.
[12]谢榕,李玉荣,李成会,等.河北禽源致病性大肠杆菌耐药性、毒力基因及超广谱β-内酰胺酶分析[J].中国家禽,2017,39(12):57-60.
[13]张利勃,刘延庆,乔长涛,等.鸡源性大肠杆菌产超广谱β-内酰胺酶基因型分布[J].中国农学通报,2011,27(20):76-78.
[14]曲志娜,刘红玉,王娟,等.青岛地区产ESBLs鸡源大肠杆菌耐药性调查与优势基因型分析[J].中国农业科学,2015(10):2058-2066.
[15]曹敏,谭艾娟,吕世明,等.贵州部分地区猪源大肠杆菌耐药性分析及ESBLs基因型检测[J].中国畜牧兽医,2016(4):1098-1104.
[16]Li B,Sun J Y,Liu Q Z,et al.High prevalence of CTX-Mβ-lactamases in faecal Escherichia coli strains from healthy humans in Fuzhou,China[J].Scand J Infect Dis,2011,43:170-174.
[17]Muzaheed,Yohei Doi J M,Adams-Haduch J M,et al.Faecal carriage of CTX-M-15 producing Klebsiella pneumoniae in patients with acute gastroenteritis[J].Indian J Med Res,2009,129(5):599-602.
[18]Weill F X,Demartin M,Laetitia Fabre L,et al.Extendedspectrum-β-lactamase(TEM-52)-producing strains of Salmonella enterica of various serotypes isolated in France[J].J Clin Microbiol,2004,42:3359-3362.
[19]Leverstein-van Hall M A,Dierikx C M,Cohen Stuart J,et al.Dutch patients,retail chicken meat and poultry share the same ESBL genes,plasmids and strains[J].Clin Microbiol Infect,2011,17(6):873-880.
[20]Liu W,Chen L,Li H,et al.Novel CTX-Mβ-lactamase genotype distribution and spread into multiple species of Enterobacteriaceae in Changsha,Southern China[J].J Antimicrob Chemother,2009,63:895-900.
[21]Barlow M,Reik R A,Jacobs S D,et al.High rate of mobilization for bla(CTX-M)s[J].Emerg Infect Dis,2008,14(3):423-428.
[22]Shiraki Y,Shibata N,Doi Y,et al.Escherichia coli producing CTX-M-2 beta-lactamase in cattle,Japan[J].Emerg Infect Dis,2004,10(1):69-75.
[23]Meireles D,Leite-Martins L,Bessa L J,et al.Molecular characterization of quinolone resistance mechanisms and extendedspectrum beta-lactamase production in Escherichia coli isolated from dogs[J].Comp Immunol Microbiol Infect Dis,2015,41:43-48.
[24]Carvalho A C,Barbosa A V,Arais L R,et al.Resistance patterns,ESBL genes,and genetic relatedness of Escherichia coli from dogs and owners[J].Braz J Microbiol,2016,47(1):150-158.
[25]宾羽琳,王萌,陈艳,等.食源性产超广谱β-内酰胺酶大肠埃希菌的分布及其同源性研究[J].中国感染控制杂志,2016,15(12):907-912.