枣树遗传转化研究进展
|
摘要
【目的】枣树是中国第一大干果树种,具有重要的经济价值和生态价值。枣树长期面临着病害严重、育种缓慢等问题,急需定向培育优良品种。【方法】枣树遗传转化一般使用农杆菌介导法,通过对遗传转化过程中的各因素进行优化,可以有效提高遗传转化效率。得到转基因植株后要验证目的基因是否整合到植物基因组并能够发挥预期的功能,验证通过后再进行后续的炼苗移栽等工作。【结果】通过枣树遗传转化试验,可以定向改良枣树品种,缩短育种年限,培育出符合实际需求的新品种。【结论】综述中国枣树遗传转化的方法和成果,总结比较枣树遗传转化过程中各个影响因素的作用,为利用基因工程手段进行枣树品种改良提供借鉴。
【Objective】Jujube is the largest dried fruit tree species in China and has important economic and ecological values.Jujube trees are faced with long-term problems such as serious diseases and slow breeding,and it is urgent to cultivate new cultivars.【Methods】Agrobacterium-mediated transformation is commonly used in the genetic transformation of Chinese jujube.Through optimization of various factors in the genetic transformation process,the efficiency of genetic transformation can be effectively improved.After obtaining the transgenic plant,it is necessary to verify whether the target gene is integrated into the plant genome and can perform its intended function.After the verification is passed,the subsequent hardening seedling transplanting are performed.【Results】Through the genetic transformation experiment of jujube tree,the jujube tree species can be improved,the breeding period can be shortened,and new varieties can be cultivated to meet the actual needs.【Conclusion】This article reviews the methods and results of genetic transformation of jujube trees in China,summarizes and compares the effects of various influencing factors in the process of genetic transformation of jujube trees,and provides references for the use of genetic engineering methods for jujube tree species improvement.
【Objective】Jujube is the largest dried fruit tree species in China and has important economic and ecological values.Jujube trees are faced with long-term problems such as serious diseases and slow breeding,and it is urgent to cultivate new cultivars.【Methods】Agrobacterium-mediated transformation is commonly used in the genetic transformation of Chinese jujube.Through optimization of various factors in the genetic transformation process,the efficiency of genetic transformation can be effectively improved.After obtaining the transgenic plant,it is necessary to verify whether the target gene is integrated into the plant genome and can perform its intended function.After the verification is passed,the subsequent hardening seedling transplanting are performed.【Results】Through the genetic transformation experiment of jujube tree,the jujube tree species can be improved,the breeding period can be shortened,and new varieties can be cultivated to meet the actual needs.【Conclusion】This article reviews the methods and results of genetic transformation of jujube trees in China,summarizes and compares the effects of various influencing factors in the process of genetic transformation of jujube trees,and provides references for the use of genetic engineering methods for jujube tree species improvement.
引文
[1]曲泽州,王惠.中国果树志·枣卷[M].北京:中国农业出版社,1993
[2]刘孟军,王玖瑞,刘平,赵锦,赵智慧,代丽,李宪松,刘志国.中国枣生产与科研成就及前沿进展[J].园艺学报,2015,42(9):1683-1698
[3]刘孟军.枣产业转型期面临的挑战与对策[J].中国果树,2018(1):1-4
[4]韩金声.北方果树病害及其防治[M].天津:天津科学技术出版社,1979
[5]祁业凤,刘孟军.两个胚败育率不同的枣品种果实生育期内源激素的变化[J].园艺学报,2004,31(6):800-802
[6]闫超,刘平,刘孟军,王玖瑞,代丽,李登科,孔得仓.枣(Ziziphusjujuba Mill.)品种结实特性研究[J].植物遗传资源学报,2009,10(1):121-125
[7]谢碧霞.枣树丰产栽培与加工技术[M].北京:中国林业出版社,1993
[8]王德,熊仁次,吴翠云,高疆生.枣品种选育的研究进展[J].塔里木大学学报,2016,28(03):109-118
[9]王关林,方宏筠.果树基因工程研究进展及展望[J].中国果树,2002(3):43-47
[10]曾黎辉,吕柳新.木本果树遗传转化研究进展[J].果树学报,2002,19(3):191-198
[11]文亚峰,原勤勤,何钢.枣树分子育种研究进展[J].经济林研究,2010,28(2):126-130
[12]何业华,林良斌,熊兴华,何钢,林顺权,陈建华.根癌农杆菌介导的枣树遗传转化系统的建立[J].分子植物育种,2003(5):683-686
[13]何业华,熊兴华,林顺权,吴会桃,林良斌,何钢,陈建华.根癌农杆菌介导反义ACC合成酶基因对枣树的转化[J].湖南农业大学学报:自然科学版,2004,30(1):33-36
[14]Gu X,Meng H G,Zhang J.Agrobacterium-mediated transformation of the winter jujube(Zizyphusjujuba Mill.)[J].Plant Cell Tissue&Organ Culture,2008,94(1):23-32
[15]黄建.农杆菌介导S6PDH基因转化枣树(Zyziphus jujube Mill.)的研究[D].杨凌:西北农林科技大学,2006
[16]郝征.农杆菌介导的枣树遗传转化研究[D].保定:河北农业大学,2012
[17]Luo Z,Guo H,Yang Y,YangM,MaL,WangY.Heterologous overexpression of resveratrol synthase(PcPKS5)gene enhances antifungal and mite aversion by resveratrol accumulation[J].European Journal of Plant Pathology,2015,142(3):547-556
[18]郭辉力.4-香豆酰辅酶A连接酶(4CL)-芪合酶(STS)基因融合、表达、催化活性鉴定及其在枣树中的过表达[D].北京:北京林业大学,2016
[19]Gelvin S B.Improving plant genetic engineering by manipulating the host[J].Trends in Biotechnology,2003,21(3):95-98
[20]王关林.植物基因工程[M].北京:科学出版社,2014
[21]巩振辉.园艺植物生物技术[M].北京:科学出版社,2009
[22]何业华,伍成厚.枣树愈伤组织培养时不定芽的分化[J].中南林业科技大学学报,1998(3):44-50
[23]张志宏,方宏筠,景士西,王关林,吴禄平,朱祯.苹果主栽品种高效遗传转化系统的建立及其影响因子的研究[J].遗传学报,1998(2):160-165
[24]秦玲,李嘉瑞,李明,韩礼星,黄贞光,赵改荣.影响苹果离体叶片早期转化效率因素的研究[J].西北植物学报,2004,24(1):25-30
[25]董喜才,杜建中,王安乐,魏国英,陈朝辉.乙酰丁香酮在植物转基因研究中的作用[J].中国农学通报,2011,27(5):292-299
[26]Nakano Y.Effect of Acetosyringone on Agrobacterium-mediated Transformation of Eustoma grandiflorum Leaf Disks[J].Japan Agricultural Research Quarterly:JARQ,2017,51(4):351-355
[27]Srinivasan M,Kumar K,Kumutha K.Influence of acetosyringone concentration on induction of carrot hairy root by Agrobacterium rhizogenes[J].African Journal of Microbiology Research,2014,8(26):2486-2491
[28]Chen B C.Agrobacterium tumifaciens-mediated Transformationfor Toreniafournieri[J].Journal of Agricultural Biotechnology,2005(3):299-303
[29]Mathews H,Dewey V,Wagoner W,BestwickR.Molecular and cellular evidence of chimaeric tissues in primary transgenics and elimination of chimaerism through improved selection protocols[J].Transgenic Research,1998,7(2):123-129
[30]刘娟,汤浩茹,王小蓉,罗娅.硝酸银在植物离体培养中的应用之研究进展[J].中国农学通报,2007(10):400-406
[2]刘孟军,王玖瑞,刘平,赵锦,赵智慧,代丽,李宪松,刘志国.中国枣生产与科研成就及前沿进展[J].园艺学报,2015,42(9):1683-1698
[3]刘孟军.枣产业转型期面临的挑战与对策[J].中国果树,2018(1):1-4
[4]韩金声.北方果树病害及其防治[M].天津:天津科学技术出版社,1979
[5]祁业凤,刘孟军.两个胚败育率不同的枣品种果实生育期内源激素的变化[J].园艺学报,2004,31(6):800-802
[6]闫超,刘平,刘孟军,王玖瑞,代丽,李登科,孔得仓.枣(Ziziphusjujuba Mill.)品种结实特性研究[J].植物遗传资源学报,2009,10(1):121-125
[7]谢碧霞.枣树丰产栽培与加工技术[M].北京:中国林业出版社,1993
[8]王德,熊仁次,吴翠云,高疆生.枣品种选育的研究进展[J].塔里木大学学报,2016,28(03):109-118
[9]王关林,方宏筠.果树基因工程研究进展及展望[J].中国果树,2002(3):43-47
[10]曾黎辉,吕柳新.木本果树遗传转化研究进展[J].果树学报,2002,19(3):191-198
[11]文亚峰,原勤勤,何钢.枣树分子育种研究进展[J].经济林研究,2010,28(2):126-130
[12]何业华,林良斌,熊兴华,何钢,林顺权,陈建华.根癌农杆菌介导的枣树遗传转化系统的建立[J].分子植物育种,2003(5):683-686
[13]何业华,熊兴华,林顺权,吴会桃,林良斌,何钢,陈建华.根癌农杆菌介导反义ACC合成酶基因对枣树的转化[J].湖南农业大学学报:自然科学版,2004,30(1):33-36
[14]Gu X,Meng H G,Zhang J.Agrobacterium-mediated transformation of the winter jujube(Zizyphusjujuba Mill.)[J].Plant Cell Tissue&Organ Culture,2008,94(1):23-32
[15]黄建.农杆菌介导S6PDH基因转化枣树(Zyziphus jujube Mill.)的研究[D].杨凌:西北农林科技大学,2006
[16]郝征.农杆菌介导的枣树遗传转化研究[D].保定:河北农业大学,2012
[17]Luo Z,Guo H,Yang Y,YangM,MaL,WangY.Heterologous overexpression of resveratrol synthase(PcPKS5)gene enhances antifungal and mite aversion by resveratrol accumulation[J].European Journal of Plant Pathology,2015,142(3):547-556
[18]郭辉力.4-香豆酰辅酶A连接酶(4CL)-芪合酶(STS)基因融合、表达、催化活性鉴定及其在枣树中的过表达[D].北京:北京林业大学,2016
[19]Gelvin S B.Improving plant genetic engineering by manipulating the host[J].Trends in Biotechnology,2003,21(3):95-98
[20]王关林.植物基因工程[M].北京:科学出版社,2014
[21]巩振辉.园艺植物生物技术[M].北京:科学出版社,2009
[22]何业华,伍成厚.枣树愈伤组织培养时不定芽的分化[J].中南林业科技大学学报,1998(3):44-50
[23]张志宏,方宏筠,景士西,王关林,吴禄平,朱祯.苹果主栽品种高效遗传转化系统的建立及其影响因子的研究[J].遗传学报,1998(2):160-165
[24]秦玲,李嘉瑞,李明,韩礼星,黄贞光,赵改荣.影响苹果离体叶片早期转化效率因素的研究[J].西北植物学报,2004,24(1):25-30
[25]董喜才,杜建中,王安乐,魏国英,陈朝辉.乙酰丁香酮在植物转基因研究中的作用[J].中国农学通报,2011,27(5):292-299
[26]Nakano Y.Effect of Acetosyringone on Agrobacterium-mediated Transformation of Eustoma grandiflorum Leaf Disks[J].Japan Agricultural Research Quarterly:JARQ,2017,51(4):351-355
[27]Srinivasan M,Kumar K,Kumutha K.Influence of acetosyringone concentration on induction of carrot hairy root by Agrobacterium rhizogenes[J].African Journal of Microbiology Research,2014,8(26):2486-2491
[28]Chen B C.Agrobacterium tumifaciens-mediated Transformationfor Toreniafournieri[J].Journal of Agricultural Biotechnology,2005(3):299-303
[29]Mathews H,Dewey V,Wagoner W,BestwickR.Molecular and cellular evidence of chimaeric tissues in primary transgenics and elimination of chimaerism through improved selection protocols[J].Transgenic Research,1998,7(2):123-129
[30]刘娟,汤浩茹,王小蓉,罗娅.硝酸银在植物离体培养中的应用之研究进展[J].中国农学通报,2007(10):400-406