固定酶底物法与多管发酵法测定地表水耐热大肠菌群的综合效果比较
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摘要
通过比较固定酶底物法与多管发酵法的检测效果,为监测站耐热大肠菌群的检测方法选择提供技术支持。2018年11月～2019年3月,选取广州市主要饮用水水源地和水源调查点采集的地表水为样本,用固定酶底物法与多管发酵法两种方法分别对51份水样中耐热大肠菌群进行比对试验检测。结果表明:固定酶底物法与多管发酵法相比,在检测一致性方面无统计学上的显著性差异,但固定酶底物法具有检测时间短、效率高、人力成本较低、结果更加精确、安全性较好等优势,既可作为地表水常规检测方法,又可用作应急、灾害时监测的快速筛查方法。
Comparing detection results of immobilized enzyme substrate method and multiple tube fermentation technique could provide technical support for the selection of heat-resistant coliform detection methods in monitoring stations. The surface water samples were collected from different drinking water reservoir and water source sites during November 2018 to March 2019. 51 samples were carried to test the existence of thermotolerant coliform bacteria by immobilized enzyme substrate method and multiple tube fermentation technique. Comparing the results of large number of parallel water samples which were tested by the above two methods, results showed that there was no statistically significant difference. However, immobilized enzyme substrate method has some advances, such as the shorter detection time, the higher efficiency, the lower labor cost and the safer performance, particularly, the results could be more precise. It could be used as a rapid screening method for routine surface water detection or emergency and disaster monitoring.
Comparing detection results of immobilized enzyme substrate method and multiple tube fermentation technique could provide technical support for the selection of heat-resistant coliform detection methods in monitoring stations. The surface water samples were collected from different drinking water reservoir and water source sites during November 2018 to March 2019. 51 samples were carried to test the existence of thermotolerant coliform bacteria by immobilized enzyme substrate method and multiple tube fermentation technique. Comparing the results of large number of parallel water samples which were tested by the above two methods, results showed that there was no statistically significant difference. However, immobilized enzyme substrate method has some advances, such as the shorter detection time, the higher efficiency, the lower labor cost and the safer performance, particularly, the results could be more precise. It could be used as a rapid screening method for routine surface water detection or emergency and disaster monitoring.
引文
[1] 生活饮用水标准检验方法:GB /T 5750—2006 [S].
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[5] REGNAULT B,MARTIN-DELAUTRE S,LEJAY-COLLIN M,et al.Oligonucleotide probe for the visualization of Escherichia coli/Escherichia fergusonii cells by in situ hybridization:Specificity and potential applications [J].Research in Microbialogy,2000(7):521-533.[5] 汤琳,李备军,龚海燕,等.粪耐热大肠菌群固定酶底物法在环境应急监测中的应用[J].环境监测管理与技术,2013,25(6):56-59.
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[2] 国家环境保护总局.水和废水监测分析方法[M].北京:中国环境科学出版社,2002:701-706.
[3] DEGRANGE V,RARDIN R.Detection and counting of nitrobacter population in soil by PCR[J].Applied Environmental Microbiology,1995,61(6):2093-2098.
[4] 王建龙.PCR技术检测水体中耐热大肠菌群的研究[J].中国生物工程杂志,2004,24(7):60-64.
[5] REGNAULT B,MARTIN-DELAUTRE S,LEJAY-COLLIN M,et al.Oligonucleotide probe for the visualization of Escherichia coli/Escherichia fergusonii cells by in situ hybridization:Specificity and potential applications [J].Research in Microbialogy,2000(7):521-533.[5] 汤琳,李备军,龚海燕,等.粪耐热大肠菌群固定酶底物法在环境应急监测中的应用[J].环境监测管理与技术,2013,25(6):56-59.
[6] 水质总耐热大肠菌群、耐热大肠菌群、大肠埃希氏菌的测定固定酶底物法:HJ 1001—2018 [S].
[7] 水质耐热大肠菌群的测定酶底物法:DBJ440100/T 276—2016 [S].
[8] 汤琳.粪耐热大肠菌群固定酶底物法在环境监测中的应用研究[J].环境监控与预警,2014,6(3):32-35.
[9] 高瑞坤,汤琳,付强.水中粪耐热大肠菌群快速检测方法-固定底物固定酶底物法与多管发酵法的比较[J].中国环境监测,2008(4):39-41.
[10] 刘瑞.固定底物固定酶底物法与环境标准方法测定粪耐热大肠菌群效果比较[C].北京:中国环境科学学会科学与技术年会,2017.
[11] 江磊.水体中粪大肠菌群的悬沙吸附特性与底泥交换过程研究[D].北京:清华大学,2015.
[12] 庞宇辰,席劲瑛,张彤,等.浊度与微生物指标在污水再生处理系统中的去除特性及相关性[J].环境工程学报,2014,8(10):4203-4208.
[13] 张静,杨正炎,杨湘霞.生活污水浑浊度与CODCr、BOD5及粪大肠菌群浓度的关系[J].现代预防医学,2004(4):505-506,512.
[14] 濮晨熹,郭大滨,胡沔,等.颗粒物的庇护作用对紫外线消毒效果的影响[J].中国给水排水,2017,33(13):73-76.