不同盐度生活污水硝化及N_2O释放特性
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摘要
以14 L序批式活性污泥反应器(SBR)处理含盐生活污水,控制曝气体积流量60 L/h、时间300 min,考察不同盐度(NaCl)SBR内微生物活性变化,并确定反应器脱氮及N_2O释放特性。结果表明,盐度对各菌群抑制程度亚硝态氮氧化菌(NOB)>氨氧化菌(AOB)>碳氧化菌。盐度<10 g/L,AOB和NOB受抑制程度较低,而N_2O还原受明显抑制,N_2O产率由盐度0时的5.14%增至10 g/L时的7.96%。盐度增至20 g/L,AOB和NOB均受到明显抑制,系统内亚硝化率达90%以上。系统淘洗出NOB,由全程硝化转变为短程硝化过程。NO_2~--N大量积累和AOB相对含量增加,为低含氧条件下AOB的好氧反硝化提供了条件,高盐度对氧化亚氮还原酶活性抑制也导致了系统N_2O释放量增加。
Using a 14 L sequencing batch activated sludge reactor(SBR) to treat salt containing domestic wastewater, with the aeration rate of 60 L/h and the aeration time of 300 min. the change of microbial activity in SBR with different salinity was studied, and the denitrification and N_2O release properties of the reactor were determined. The results showed that, The degree of salinity inhibition on the flora was NOB> AOB> carbon oxidation bacteria. With the salinity less than 10 g/L, AOB and NOB were less inhibited, while the N_2O reduction was significantly inhibited with the N_2O yield increasing from5.14% at salinity of 0 to 7.96% at salinity of 10 g/L. Both AOB and NOB were significantly inhibited at the salinity of 20 g/L, with the nitrite accumulation rate reached more than 90%. The NOB was continuously washed out the system, the whole nitrification translated to the partial nitrification. Large amount of NO_2~- accumulation and the relative content of AOB increased provided conditions for aerobic denitrification of AOB under low oxygen condition.The inhibition of nitrous oxide reductase activity by high salinity also led to an increase of N_2O release in the system.
Using a 14 L sequencing batch activated sludge reactor(SBR) to treat salt containing domestic wastewater, with the aeration rate of 60 L/h and the aeration time of 300 min. the change of microbial activity in SBR with different salinity was studied, and the denitrification and N_2O release properties of the reactor were determined. The results showed that, The degree of salinity inhibition on the flora was NOB> AOB> carbon oxidation bacteria. With the salinity less than 10 g/L, AOB and NOB were less inhibited, while the N_2O reduction was significantly inhibited with the N_2O yield increasing from5.14% at salinity of 0 to 7.96% at salinity of 10 g/L. Both AOB and NOB were significantly inhibited at the salinity of 20 g/L, with the nitrite accumulation rate reached more than 90%. The NOB was continuously washed out the system, the whole nitrification translated to the partial nitrification. Large amount of NO_2~- accumulation and the relative content of AOB increased provided conditions for aerobic denitrification of AOB under low oxygen condition.The inhibition of nitrous oxide reductase activity by high salinity also led to an increase of N_2O release in the system.
引文
[1]RODRIGUEZ-CABALLERO A,AYMERICH I,RICARDO M.et al.Minimizing N2O emissions and carbon footprint on a full-scale activated sludge sequencing batch reactor[J].Water Resarch,2015,71(15):1-10.
[2]AHN J H,KIM S,PARK H,et al.N2O emissions from activated sludge processes,2008-2009:results of a national monitoring survey in the United States[J].Environ Sci Technol,2010,44(12):4505-4511.
[3]PAN Y T,VAN DEN AKKER B,YE L,et al.Unravelling the spatial variation of nitrous oxide emissions frm a step-feed plug-flow full scale wastewater treatment plant[J].Scientific Reports,2016,6(1):60-70.
[4]EGGLESTON S,BUENDIA L,MIWA K,et al.2006 IPCC guidelines for national greenhouse gas inventories[C].IGES,2006:24-26.
[5]YE L,NI B J,LAW Y Y,et al.A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators[J].Water Research,2014,48(1):257-268.
[6]ZHAO W,WANG Y Y,LIN X,et al.Identification of the salinity effect on N2O production pathway during nitrification:Using stepwise inhibition and15N isotope labeling methods[J].Chemical Engineering Journal,2014,253(1):418-426.
[7]巩有奎,王淑莹,王莎莎,等,碳氮比对短程反硝化过程中N2O产生的影响[J].化工学报,2011,62(7):2049-2054.
[8]URFER D,HUCK P M.Measurement of biomass activity in drinking water biofilters using a respirometric method[J].Wat Res,2001,35(6):1469-1477.
[9]巩有奎,王赛,彭永臻等,生活污水不同生物脱氮过程中N2O产量及控制[J].化工学报,2010,61(5):1286-1292.
[10]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[11]COPP J B,SPANJERS H.Simulation of respirometry-based detection and mitigation of activated sludge toxicity[J].Control Engineering Practice,2004,12(1):305-313
[12]TSUNEDA S,MIKAMI M,KIMOCHI Y.et al.Effect of salinity on nitrous oxide emission in the biological nitrogen removal process for industrial wastewater[J].Journal of Hazardous Materials,2005,119(1/3):93-98
[13]YANG Q,LIU X H,PENG C Y,et al.N2O production during nitrogen removal via nitrite from domestic wastewater:Main sources and control method[J].Environental Science&Technology,2009,43(24):9400-9406.
[14]SHAW L J,NICOL G W,SMITH Z,et al.Nitrosospira sp.can produce nitrous oxide via a nitrifier denitrification pathway[J].Environmental Microbiology,2006,8(2):214-222.
[15]BEVAP G,BAGGS E M,PROSSER R J I.Phylogeny of nitrite reductase(nirK)and nitric oxide reductase(norB)genes from Nitrosospira species isolated from soil[J].FEMS Microbio-Logy Letters,2006,266(1):83-89
[16]KAMPSCHREUR M J,TEMMINK H,KLEEREBEZEM R.et al.Nitrous oxide emission during wastewater treatment[J].Water Research,2009,43(17):4093-4103.
[17]PENG L,NI B J,YE L,et al.The combined effect of dissolved oxygen and nitrite on N2O production by ammonia oxidizing bacteria in an enriched nitrifying sludge[J].Water Research,2015,73(1):29-36.
[2]AHN J H,KIM S,PARK H,et al.N2O emissions from activated sludge processes,2008-2009:results of a national monitoring survey in the United States[J].Environ Sci Technol,2010,44(12):4505-4511.
[3]PAN Y T,VAN DEN AKKER B,YE L,et al.Unravelling the spatial variation of nitrous oxide emissions frm a step-feed plug-flow full scale wastewater treatment plant[J].Scientific Reports,2016,6(1):60-70.
[4]EGGLESTON S,BUENDIA L,MIWA K,et al.2006 IPCC guidelines for national greenhouse gas inventories[C].IGES,2006:24-26.
[5]YE L,NI B J,LAW Y Y,et al.A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators[J].Water Research,2014,48(1):257-268.
[6]ZHAO W,WANG Y Y,LIN X,et al.Identification of the salinity effect on N2O production pathway during nitrification:Using stepwise inhibition and15N isotope labeling methods[J].Chemical Engineering Journal,2014,253(1):418-426.
[7]巩有奎,王淑莹,王莎莎,等,碳氮比对短程反硝化过程中N2O产生的影响[J].化工学报,2011,62(7):2049-2054.
[8]URFER D,HUCK P M.Measurement of biomass activity in drinking water biofilters using a respirometric method[J].Wat Res,2001,35(6):1469-1477.
[9]巩有奎,王赛,彭永臻等,生活污水不同生物脱氮过程中N2O产量及控制[J].化工学报,2010,61(5):1286-1292.
[10]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[11]COPP J B,SPANJERS H.Simulation of respirometry-based detection and mitigation of activated sludge toxicity[J].Control Engineering Practice,2004,12(1):305-313
[12]TSUNEDA S,MIKAMI M,KIMOCHI Y.et al.Effect of salinity on nitrous oxide emission in the biological nitrogen removal process for industrial wastewater[J].Journal of Hazardous Materials,2005,119(1/3):93-98
[13]YANG Q,LIU X H,PENG C Y,et al.N2O production during nitrogen removal via nitrite from domestic wastewater:Main sources and control method[J].Environental Science&Technology,2009,43(24):9400-9406.
[14]SHAW L J,NICOL G W,SMITH Z,et al.Nitrosospira sp.can produce nitrous oxide via a nitrifier denitrification pathway[J].Environmental Microbiology,2006,8(2):214-222.
[15]BEVAP G,BAGGS E M,PROSSER R J I.Phylogeny of nitrite reductase(nirK)and nitric oxide reductase(norB)genes from Nitrosospira species isolated from soil[J].FEMS Microbio-Logy Letters,2006,266(1):83-89
[16]KAMPSCHREUR M J,TEMMINK H,KLEEREBEZEM R.et al.Nitrous oxide emission during wastewater treatment[J].Water Research,2009,43(17):4093-4103.
[17]PENG L,NI B J,YE L,et al.The combined effect of dissolved oxygen and nitrite on N2O production by ammonia oxidizing bacteria in an enriched nitrifying sludge[J].Water Research,2015,73(1):29-36.