北部湾海域球形棕囊藻遗传多样性分析
|
摘要
南海北部湾海域近年来连年暴发球形棕囊藻赤潮,严重威胁近海生态安全,亟待探明赤潮成因。球形棕囊藻是一种广泛分布于温带至热带海域的有害藻华微藻,具有明显的种下遗传分化。为了解北部湾海域球形棕囊藻的遗传多样性及其对赤潮藻种来源的指示意义,本研究针对分离于北部湾海域赤潮发生期间的4株棕囊藻,以核糖体28S大亚基rRNA基因(28SrDNA)D1—D2区和转录间隔区(ITS区)为靶区构建克隆文库,通过测序分析了北部湾海域球形棕囊藻的遗传多样性。结果表明,从北部湾分离的4株棕囊藻均为球形棕囊藻,但不同年份分离的球形棕囊藻藻株之间存在明显的遗传差异。部分球形棕囊藻藻株(PG2015和PG2017)的不同克隆间也存在遗传差异,这种遗传差异在由单一藻细胞建立的克隆培养系中仍存在,表明遗传差异是来自细胞内多拷贝的基因序列,可能源自不同地理种群球形棕囊藻之间的基因交流。与ITS区相比,28SrDNAD1—D2区能更好地反映球形棕囊藻遗传多样性状况,具有作为分子标记指示球形棕囊藻地理种群的潜力,但仍需深入研究。研究结果深化了对北部湾球形棕囊藻遗传多样性的认识,有望为进一步解析北部湾海域球形棕囊藻赤潮原因种来源提供判据。
Red tides of Phaeocystis globosa were recorded in the Beibu Gulf, the South China Sea in the last five years,posing significant impacts on marine environment and ecosystems. P. globosa is a major red-tide causative species,distributes from temperate to tropical waters around the world, and features rich intraspecies genetic diversity. To understand the status of genetic diversity of P. globosa in the Beibu Gulf and its potential role to reveal the origin of red-tide-forming P. globosa, four strains of Phaeocystis were isolated from the gulf during Phaeocystis blooms from 2015 to 2017 and examined. Targeting at the 28 S ribosomal rRNA gene(28 SrDNA) and the ITS region, clone libraries were established for the four strains of P. globosa and sequenced. Based on the phylogenetic analysis of the 28 D1—D2 region and ITS region, all of the four strains of Phaeocystis could be identified as P. globosa, and significant genetic diversity was revealed in P. globosa. Strains isolated at different years from the Beibu Gulf had different genetic information. High genetic diversity was found not only among strains of P. globosa but also within a clone culture established from a single cell of P. globosa. It implies that the genetic diversity of some P. globosa strains exists within multiple copies of targeted genes within a single cell, which is probably due to the gene exchange among different geographical populations of P. globosa. Compared to the ITS region, the 28 SrDNA D1—D2 region is a better choice to represent the intraspecies genetic diversity of P. globosa. The results primarily revealed the genetic diversity of P. globosa in the Beibu Gulf and will provide important clues of the origination of bloom-forming P. globosa in the Beibu Gulf.
Red tides of Phaeocystis globosa were recorded in the Beibu Gulf, the South China Sea in the last five years,posing significant impacts on marine environment and ecosystems. P. globosa is a major red-tide causative species,distributes from temperate to tropical waters around the world, and features rich intraspecies genetic diversity. To understand the status of genetic diversity of P. globosa in the Beibu Gulf and its potential role to reveal the origin of red-tide-forming P. globosa, four strains of Phaeocystis were isolated from the gulf during Phaeocystis blooms from 2015 to 2017 and examined. Targeting at the 28 S ribosomal rRNA gene(28 SrDNA) and the ITS region, clone libraries were established for the four strains of P. globosa and sequenced. Based on the phylogenetic analysis of the 28 D1—D2 region and ITS region, all of the four strains of Phaeocystis could be identified as P. globosa, and significant genetic diversity was revealed in P. globosa. Strains isolated at different years from the Beibu Gulf had different genetic information. High genetic diversity was found not only among strains of P. globosa but also within a clone culture established from a single cell of P. globosa. It implies that the genetic diversity of some P. globosa strains exists within multiple copies of targeted genes within a single cell, which is probably due to the gene exchange among different geographical populations of P. globosa. Compared to the ITS region, the 28 SrDNA D1—D2 region is a better choice to represent the intraspecies genetic diversity of P. globosa. The results primarily revealed the genetic diversity of P. globosa in the Beibu Gulf and will provide important clues of the origination of bloom-forming P. globosa in the Beibu Gulf.
引文
王宁,陈月琴,屈良鹄等,2000.1997粤东海域棕囊藻赤潮原因种18SrDNA基因分析.中山大学学报(自然科学版),39(1):127-128
曲凌云,吕颂辉,高春蕾等,2008.棕囊藻渤海株核糖体18SrDNA和ITS基因结构序列分析.海洋科学进展,26(2):200-206
危蔚,江天久,2005.有害赤潮生物球形棕囊藻对卤虫的毒性研究.生态科学,24(1):38-41
刘海林,章群,江启明等,2010.赤潮棕囊藻(Phaeocystis globosa)rDNA ITS区序列变异与二级结构分析.生态科学,29(5):432-437
齐雨藻,沈萍萍,王艳,2001.棕囊藻属(Phaeocystis)的分类与生活史(综述).热带亚热带植物学报,9(2):174-184
苏纪兰,2005.中国近海水文.北京:海洋出版社
李亚男,沈萍萍,黄良民等,2012.棕囊藻的分类及系统进化研究进展.生态学杂志,21(3):745-754
杨泽民,章群,谢数涛等,2004a.球形棕囊藻(Phaeocystis globosa)叶绿体psaA基因片段的序列分析.热带亚热带植物学报,12(5):435-439
杨泽民,章群,谢数涛等,2004b.Phaeocystis globosa核糖体大亚基(28S rDNA)的序列分析与分类学意义.海洋环境科学,23(3):4-8
杨泽民,章群,谢数涛等,2005.Phaeocystis globosa与Phaeocystis antarctica叶绿体psbA基因的比较.海洋科学,29(1):24-28,80
吴敏兰,2014.北部湾北部海域营养盐的分布特征及其对生态系统的影响研究.厦门:厦门大学硕士学位论文
陈月琴,王宁,周惠等,2002.棕囊藻赤潮原因种的分子鉴定和起源分析.海洋学报,24(6):99-103
陈丽芬,章群,骆育敏等,2003.18S rDNA序列分析鉴定棕囊藻香港株P2为球形棕囊藻.生态科学,22(4):349-350
陈振华,2014.北部湾环流季节变化的数值模拟与动力机制分析.青岛:中国海洋大学博士学位论文
陈菊芳,徐宁,江天久等,1999.中国赤潮新记录种--球形棕囊藻(Phaeocystis globosa).暨南大学学报(自然科学版),20(3):124-129
侍茂崇,2014.北部湾环流研究述评.广西科学,21(4):313-324
赵越,于仁成,张清春等,2019.北部湾海域微型、微微型浮游植物类群季节变化及其与棕囊藻赤潮的关系初探.海洋与湖沼,50(3):590-600
黄天吾,2012.球形棕囊藻囊体形态特征及光和钙离子对囊体形成和囊体细胞分布的影响.广州:暨南大学硕士学位论文
黄长江,董巧香,郑磊,1999.1997年底中国东南沿海大规模赤潮原因生物的形态分类与生态学特征.海洋与湖沼,30(6):581-590
覃仙玲,赖俊翔,陈波等,2016.棕囊藻北部湾株的18SrDNA分子鉴定.热带亚热带植物学报,24(2):176-181
Blomster J,Maggs C A,Stanhope M J,1998.Molecular and morphological analysis of Enteromorpha intestinalis and E.compressa(Chlorophyta)in the British Isles.J Phycol,34(2):319-340
Charlson R J,Lovelock J E,Andreae M O et al,1987.Oceanic phytoplankton,atmospheric sulphur,cloud albedo and climate.Nature,326(6114):655-661
Chen Y Q,Shao P,Wang N et al,2003.Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITSsequence analysis.Acta Oceanol Sin,22(2):243-253
Chen Y Q,Wang N,Zhang P et al,2002.Molecular evidence identifies bloom-forming Phaeocystis(Prymnesiophyta)from coastal waters of southeast China as Phaeocystis globosa.Biochem Syst Ecol,30(1):15-22
Decelle J,Probert I,Bittner L et al,2012.An original mode of symbiosis in open ocean plankton.P Natl Acad Sci USA,109(44):18000-18005
Doan N H,Nguyen N L,Nguyen M A et al,2008.Red tides in south central coast of Viet Nam:biodiversity and planktonic community structure consequences.Poster Presentation at XIII HAB Conference,Hong Kong,November 2008
Hai D N,Lam N N,Dippner J W et al,2010.Development of Phaeocystis globosa blooms in the upwelling waters of the South Central coast of Viet Nam.J Marine Syst,83(3/4):253-261
Kornmann P,2011.Chapter 10 Phaeocystis globosa:autecological data and bloom phenomena.In:Auteology and Blooms,168-194
Lange M,1996.Phylogeny and taxonomy of the genus Phaeocystis.Germany:University of Bremen
Lange M,Chen Y Q,Medlin L K,2002.Molecular genetic delineation of Phaeocystis species(Prymnesiophyceae)using coding and non-coding regions of nuclear and plastid genomes.Eur J Phycol,37(1):77-92
Medlin L K,Zingone A,2007.A taxonomic review of the genus Phaeocystis.Biogeochemistry,83(1-3):3-18
Medlin L K,Lange M,Baumann M E M,1994.Genetic differentiation among 3 colony-forming species of Phaeocystis-Further evidence for the phylogeny of the prymnesiophyta.Phycologia 33(3):199-212
Peperzak L,G?bler-Schwarz S,2012.Current knowledge of the life cycles of Phaeocystis globosa and Phaeocystis antarctica(Prymnesiophyceae).J Phycol,48(3):514-517
Posada D,2016.Phylogenomics for systematic biology.Syst Biol,65(3):353-356
Qi Y Z,Chen J F,Wang Z H et al,2004.Some observations on harmful algal bloom(HAB)events along the coast of Guangdong,southern China in 1998.Hydrobiologia,512(1-3):209-214
Schoemann V,Becquevort S,Stefels J,Rousseau V,Lancelot C,2005.Phaeocystis blooms in the global ocean and their controlling mechanisms:a review.J Sea Res,53(1-2):43-66
Shi M C,Chen C S,Xu Q C et al,2002.The role of Qiongzhou Strait in the seasonal variation of the South China Sea circulation.J Phys Oceanogr,32(1):103-121
Vaulot D,Birrien J L,Marie D et al,1994.Morphology,ploidy,pigment composition,and genome size of cultured strains of Phaeocystis(Prymnesiophyceae).J Phycol,30(6):1022-1035
Wassmann P,Ratkova T,Reigstad M,2005.The contribution of single and colonial cells of Phaeocystis pouchetii to spring and summer blooms in the north-eastern North Atlantic.Harmful Algae,4(5):823-840
Whipple S J,Patten B C,Verity P G,2005.Life cycle of the marine alga Phaeocystis:a conceptual model to summarize literature and guide research.J Mar Syst,57(1-2):83-110
Winnepenninckx B,Backeljau T,De Wachter R,1993.Extraction of high molecular weight DNA from molluscs.Trends Genet,9(12):407
曲凌云,吕颂辉,高春蕾等,2008.棕囊藻渤海株核糖体18SrDNA和ITS基因结构序列分析.海洋科学进展,26(2):200-206
危蔚,江天久,2005.有害赤潮生物球形棕囊藻对卤虫的毒性研究.生态科学,24(1):38-41
刘海林,章群,江启明等,2010.赤潮棕囊藻(Phaeocystis globosa)rDNA ITS区序列变异与二级结构分析.生态科学,29(5):432-437
齐雨藻,沈萍萍,王艳,2001.棕囊藻属(Phaeocystis)的分类与生活史(综述).热带亚热带植物学报,9(2):174-184
苏纪兰,2005.中国近海水文.北京:海洋出版社
李亚男,沈萍萍,黄良民等,2012.棕囊藻的分类及系统进化研究进展.生态学杂志,21(3):745-754
杨泽民,章群,谢数涛等,2004a.球形棕囊藻(Phaeocystis globosa)叶绿体psaA基因片段的序列分析.热带亚热带植物学报,12(5):435-439
杨泽民,章群,谢数涛等,2004b.Phaeocystis globosa核糖体大亚基(28S rDNA)的序列分析与分类学意义.海洋环境科学,23(3):4-8
杨泽民,章群,谢数涛等,2005.Phaeocystis globosa与Phaeocystis antarctica叶绿体psbA基因的比较.海洋科学,29(1):24-28,80
吴敏兰,2014.北部湾北部海域营养盐的分布特征及其对生态系统的影响研究.厦门:厦门大学硕士学位论文
陈月琴,王宁,周惠等,2002.棕囊藻赤潮原因种的分子鉴定和起源分析.海洋学报,24(6):99-103
陈丽芬,章群,骆育敏等,2003.18S rDNA序列分析鉴定棕囊藻香港株P2为球形棕囊藻.生态科学,22(4):349-350
陈振华,2014.北部湾环流季节变化的数值模拟与动力机制分析.青岛:中国海洋大学博士学位论文
陈菊芳,徐宁,江天久等,1999.中国赤潮新记录种--球形棕囊藻(Phaeocystis globosa).暨南大学学报(自然科学版),20(3):124-129
侍茂崇,2014.北部湾环流研究述评.广西科学,21(4):313-324
赵越,于仁成,张清春等,2019.北部湾海域微型、微微型浮游植物类群季节变化及其与棕囊藻赤潮的关系初探.海洋与湖沼,50(3):590-600
黄天吾,2012.球形棕囊藻囊体形态特征及光和钙离子对囊体形成和囊体细胞分布的影响.广州:暨南大学硕士学位论文
黄长江,董巧香,郑磊,1999.1997年底中国东南沿海大规模赤潮原因生物的形态分类与生态学特征.海洋与湖沼,30(6):581-590
覃仙玲,赖俊翔,陈波等,2016.棕囊藻北部湾株的18SrDNA分子鉴定.热带亚热带植物学报,24(2):176-181
Blomster J,Maggs C A,Stanhope M J,1998.Molecular and morphological analysis of Enteromorpha intestinalis and E.compressa(Chlorophyta)in the British Isles.J Phycol,34(2):319-340
Charlson R J,Lovelock J E,Andreae M O et al,1987.Oceanic phytoplankton,atmospheric sulphur,cloud albedo and climate.Nature,326(6114):655-661
Chen Y Q,Shao P,Wang N et al,2003.Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITSsequence analysis.Acta Oceanol Sin,22(2):243-253
Chen Y Q,Wang N,Zhang P et al,2002.Molecular evidence identifies bloom-forming Phaeocystis(Prymnesiophyta)from coastal waters of southeast China as Phaeocystis globosa.Biochem Syst Ecol,30(1):15-22
Decelle J,Probert I,Bittner L et al,2012.An original mode of symbiosis in open ocean plankton.P Natl Acad Sci USA,109(44):18000-18005
Doan N H,Nguyen N L,Nguyen M A et al,2008.Red tides in south central coast of Viet Nam:biodiversity and planktonic community structure consequences.Poster Presentation at XIII HAB Conference,Hong Kong,November 2008
Hai D N,Lam N N,Dippner J W et al,2010.Development of Phaeocystis globosa blooms in the upwelling waters of the South Central coast of Viet Nam.J Marine Syst,83(3/4):253-261
Kornmann P,2011.Chapter 10 Phaeocystis globosa:autecological data and bloom phenomena.In:Auteology and Blooms,168-194
Lange M,1996.Phylogeny and taxonomy of the genus Phaeocystis.Germany:University of Bremen
Lange M,Chen Y Q,Medlin L K,2002.Molecular genetic delineation of Phaeocystis species(Prymnesiophyceae)using coding and non-coding regions of nuclear and plastid genomes.Eur J Phycol,37(1):77-92
Medlin L K,Zingone A,2007.A taxonomic review of the genus Phaeocystis.Biogeochemistry,83(1-3):3-18
Medlin L K,Lange M,Baumann M E M,1994.Genetic differentiation among 3 colony-forming species of Phaeocystis-Further evidence for the phylogeny of the prymnesiophyta.Phycologia 33(3):199-212
Peperzak L,G?bler-Schwarz S,2012.Current knowledge of the life cycles of Phaeocystis globosa and Phaeocystis antarctica(Prymnesiophyceae).J Phycol,48(3):514-517
Posada D,2016.Phylogenomics for systematic biology.Syst Biol,65(3):353-356
Qi Y Z,Chen J F,Wang Z H et al,2004.Some observations on harmful algal bloom(HAB)events along the coast of Guangdong,southern China in 1998.Hydrobiologia,512(1-3):209-214
Schoemann V,Becquevort S,Stefels J,Rousseau V,Lancelot C,2005.Phaeocystis blooms in the global ocean and their controlling mechanisms:a review.J Sea Res,53(1-2):43-66
Shi M C,Chen C S,Xu Q C et al,2002.The role of Qiongzhou Strait in the seasonal variation of the South China Sea circulation.J Phys Oceanogr,32(1):103-121
Vaulot D,Birrien J L,Marie D et al,1994.Morphology,ploidy,pigment composition,and genome size of cultured strains of Phaeocystis(Prymnesiophyceae).J Phycol,30(6):1022-1035
Wassmann P,Ratkova T,Reigstad M,2005.The contribution of single and colonial cells of Phaeocystis pouchetii to spring and summer blooms in the north-eastern North Atlantic.Harmful Algae,4(5):823-840
Whipple S J,Patten B C,Verity P G,2005.Life cycle of the marine alga Phaeocystis:a conceptual model to summarize literature and guide research.J Mar Syst,57(1-2):83-110
Winnepenninckx B,Backeljau T,De Wachter R,1993.Extraction of high molecular weight DNA from molluscs.Trends Genet,9(12):407
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |