棉花PsbS基因对烟草光合特性的影响
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摘要
【目的】分析光系统Ⅱ Psb S基因在不同光照条件下,光系统Ⅱ PsbS基因的功能。【方法】利用转棉花PsbS基因的烟草(Nicotiana tabacum xanthin),T3代株系及非转基因(普通野生型,wt)烟草为材料,比较两者的生物学性状(叶面积、叶绿素含量)及在不同光强(75、240、450μmol/(m~2·s))下的生理(光合作用)性状。【结果】转基因植株前期叶面积增长较快,叶绿素组成成分的含量也存在差异,叶绿素a、b含量显著高于野生型烟草,转基因烟草具有更强的光合能力,并伴随着气孔导度(Gs)、蒸腾速率(Tr)提高。【结论】转PsbS基因可以提高烟草光合能力。
【Objective】To expound the function of Ⅱ PsbS gene in photo system from the point of view of photosynthesis according to the analysis of Ⅱ PsbS gene in photosystem under different light conditions.【Method】In this experiment,cotton PsbS transgenic( Nicotiana tabacum xanthin),T3 generation lines and non-transgenic( wild-type,wt) tobacco) were used as materials to compare the biological characters( leaf area,chlorophyll content) and physiological( photosynthetic) characters under different light intensities( 75,240 and 450 μmol/( m~2·s)) of the two types of tobaccos. 【Result】The leaf area of the transgenic plants increased rapidly in the early stage,and the content of chlorophyll components also varied. The contents of chlorophyll A and B were significantly higher than those of wild type tobacco,and the transgenic tobacco had stronger photosynthetic capacity accompanied by stomatal conductance( Gs),transpiration rate( Tr) increase. 【Conclusion】The transgenic PsbS gene can enhance the photosynthetic capacity of tobacco.
【Objective】To expound the function of Ⅱ PsbS gene in photo system from the point of view of photosynthesis according to the analysis of Ⅱ PsbS gene in photosystem under different light conditions.【Method】In this experiment,cotton PsbS transgenic( Nicotiana tabacum xanthin),T3 generation lines and non-transgenic( wild-type,wt) tobacco) were used as materials to compare the biological characters( leaf area,chlorophyll content) and physiological( photosynthetic) characters under different light intensities( 75,240 and 450 μmol/( m~2·s)) of the two types of tobaccos. 【Result】The leaf area of the transgenic plants increased rapidly in the early stage,and the content of chlorophyll components also varied. The contents of chlorophyll A and B were significantly higher than those of wild type tobacco,and the transgenic tobacco had stronger photosynthetic capacity accompanied by stomatal conductance( Gs),transpiration rate( Tr) increase. 【Conclusion】The transgenic PsbS gene can enhance the photosynthetic capacity of tobacco.
引文
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[13]许中旗,黄选瑞,徐成立,等.光照条件对蒙古栎幼苗生长及形态特征的影响[J].生态学报,2009,29(3):1 121-1128.XU Zhong-qi,HUANG Xuan-rui,XU Cheng-li,et al.(2009). The impacts of light conditions on the growth and morphology of Quercus Mongolica seedlings[J]. Acta Ecologica Sinica,29(3):1,121-1,128.(in Chinese)
[14]姚新转,吕立堂,赵德刚.转拟南芥BAS1基因提高烟草光合特性[J].山地农业生物学报,2016,35(3):30-34.YAO Xin-zhuan,Lü Li-tang,ZHAO De-gang.(2016).Improving photosynthetic characteristics of tobacco plants with BAS1 gene in transgenic Arabidopsis thaliana[J]. Journal of Mountain Agriculture and Biology,35(3):30-34.(in Chinese)
[15]Funk,C.,Schrder WP,Green,B. R.,Renger,G.,&Andersson,B..(1994). The intrinsic 22 kda protein is a chlorophyll-binding subunit of photosystem II. Febs Letters,342(3):261.
[16] Krishnamoorthi,S.,Goh,K. Y.,Chong,D. Y. R.,Kapoor,R.,&Sun,A. Y. S.(1992). Thermal characterization of a thermally enhanced qfn package. Febs Letters,314(1):67-71.
[17]Li,X. P.,Olle Bjrkman,Shih,C.,Grossman,A. R.,&Niyogi,K. K.(2000). A pigment-binding protein essential for regulation of photosynthetic light harvesting. Nature, 403(6768):391-395.
[18] Horton,P.,Ruban,A. V.,&Walters,R. G.(1996).Regulation of light harvesting in green plants. Annual Review of Plant Physiology&Plant Molecular Biology,47(1):655-684.
[19]Li,Z.,Ahn,T. K.,Avenson,T. J.,Ballottari,M.,Cruz,J. A.,&Kramer,D. M.,et al.(2009). Lutein accumulation in the absence of zeaxanthin restores nonphotochemical quenching in the arabidopsis thaliana npq1 mutant. The Plant Cell Online,21(6):1,798-1,812.
[20]Li,X. P.,Gilmore,A. M.,Caffarri,S.,Bassi,R.,Golan,T.,&Kramer,D.,et al.(2004). Regulation of photosynthetic light harvesting involves intrathylakoid lumen ph sensing by the psbs protein. Journal of Biological Chemistry,279(22):22,866-22,874.
[21]Fariduddin,Q.,Hayat,S.,&Ahmad,A.(2003). Salicylic acid influences net photosynthetic rate,carboxylation efficiency,nitrate reductase activity,and seed yield inbrassica juncea. Photosynthetica(Prague),41(2):281-284.
[22]Zhang,M.,&Yang,X. E.(2014). Psbsexpression analysis in two ecotypes ofsedum alfrediiand the role ofsapsbsin cd tolerance. Russian Journal of Plant Physiology,61(4):427-433.
[23]Krah,N. M.,&Logan,B. A.(2010). Loss of PSBs expression reduces vegetative growth,reproductive output,and light-limited,but not light-saturated,photosynthesis in arabidopsis thaliana(brassicaceae)grown in temperate light environments.American Journal of Botany,97(4):644-649.
[24]高志民,于小清,郑波,等.毛竹PsbS基因的克隆及其原核表达特征研究[J].热带亚热带植物学报,2011,19(6):513-518.GAO Zhi-min,YU Xiao-qing,ZHANG Bo,et al.(2011).Cloning of PsbS gene from moso bamboo(Phyllostachys edulis)and its characterization of prokaryotic expression[J]. Journal of Tropical and Subtropical Botany,19(6):513-518.(in Chinese)
[2]Demmig-Adams,B.,Garab,G.,Iii,W. A.,&Govindjee.(2014). Non-photochemical quenching and energy dissipation in plants,algae and cyanobacteria. Springer Netherlands.
[3]Glowacka,K.,Kromdijk,J.,Kucera,K.,Xie,J.,Cavanagh,A. P.,&Leonelli,L.,et al.(2018). Photosystem ii subunit s overexpression increases the efficiency of water use in a field-grown crop. Nature Communications,9(1):868.
[4] Lou,Y.,Sun,H.,Wang,S.,Xu,H.,Li,L.,&Zhao,H.,et al.(2018). Expression and functional analysis of two psbs genes in bamboo(phyllostachys edulis). Physiologia Plantarum,163(4):459.
[5]唐媛媛,赵瑞海,汤丽魁,等.陆地棉光合系统ⅡGh PsbS基因的克隆及其表达分析[J].分子植物育种,2017,15(7):2 489-2 496.TANG Yuan-yuan,ZHAO Rui-hai,TANG Li-kui,et al.(2017). Cloning and Expression Analysis of PhotosystemⅡGhPsbS Gene in Gossypium hirsupum[J]. Molecular Plant Breeding,15(7):2,489-2,496.(in Chinese)
[6]胡根海,喻树迅.利用改良的CTAB法提取棉花叶片总RNA[J].棉花学报,2007,19(1):69-70.HU Gen-hai,YU Shu-xun.(2007). Extraction of highquality total RNA in cotton leaf with improved CTAB method[J]. Cotton Science,19(1):69-70.(in Chinese)
[7]郑凤君,华南金秋,张立猛,等.长宽法测定幼苗期烟草叶面积的校正系数[J].中国烟草科学,2015,36(6):13-16.ZHENG Feng-jun,HUA Nan-jin-qiu,ZHANG Li-meng,et al.(2015). Revision of the Length-width Method Correction Coefficient in Measuring Leaf Area of Tobacco Plants at the Seedling Stage[J]. Chinese Tobacco Science,36(6):13-16.(in Chinese)
[8]张蜀秋,韩玉珍,李云.植物生理学实验技术教程[M].科学出版社,2011.ZHANG Shu-qiu,HAN Yu-zhen,LI Yun.(2011). Techniques for Plant Physiology Experi-merit[M]. Science Press.(in Chinese)
[9]武高林,杜国祯.植物形态生长对策研究进展[J].世界科技研究与发展,2007,29(4):47-51.WU Gao-lin,DU Guo-zhen.(2007). Advances in Plant Morphological Growth Strategy[J]. World Sci-Tech R&D,29(4):47-51.(in Chinese)
[10]刘柿良,马明东,潘远智,等.不同光强对两种桤木幼苗光合特性和抗氧化系统的影响[J].植物生态学报,2012,36(10):1 062-1 074.LIU Shi-liang,MA Ming-dong,PAN Yuan-zhi,et al.(2012). Effects of light regimes on photosynthetic characteristics and antioxidant system in seedlings of two alder species[J]. Chinese Journal of Plant Ecology,36(10):1,062-1,074.(in Chinese)
[11]Koo,H. Y.,Zulfugarov,I. S.,Oh,M. H.,et al.(2004).The function of the PsbS protein in relation to nonphotochemical energy dependent quenching in rice plants. In Photosynthesis:Fundamental Aspects to Global Perspectives:30 August-03 September 2004. Edited by van der Est A,Bruce D. Montreal:Allen Press,Incorporated; 2005:527-530.
[12]李磊,曾晓芳,赵德刚.贵州地方稻种来拢小圆粒半矮秆突变体srd-1筛选及鉴定[J].核农学报,2015,29(4):629-636.LI Lei,ZENG Xiao-fang,ZHAO De-gang.(2015). Screening and Identification of a SmallRound Seed and Semi-dwarf Mutant-1 of Native Rice Variety Lailong in Guizhou[J]. Journal of Nuclear Agricultural Sciences,29(4):629-636.(in Chinese)
[13]许中旗,黄选瑞,徐成立,等.光照条件对蒙古栎幼苗生长及形态特征的影响[J].生态学报,2009,29(3):1 121-1128.XU Zhong-qi,HUANG Xuan-rui,XU Cheng-li,et al.(2009). The impacts of light conditions on the growth and morphology of Quercus Mongolica seedlings[J]. Acta Ecologica Sinica,29(3):1,121-1,128.(in Chinese)
[14]姚新转,吕立堂,赵德刚.转拟南芥BAS1基因提高烟草光合特性[J].山地农业生物学报,2016,35(3):30-34.YAO Xin-zhuan,Lü Li-tang,ZHAO De-gang.(2016).Improving photosynthetic characteristics of tobacco plants with BAS1 gene in transgenic Arabidopsis thaliana[J]. Journal of Mountain Agriculture and Biology,35(3):30-34.(in Chinese)
[15]Funk,C.,Schrder WP,Green,B. R.,Renger,G.,&Andersson,B..(1994). The intrinsic 22 kda protein is a chlorophyll-binding subunit of photosystem II. Febs Letters,342(3):261.
[16] Krishnamoorthi,S.,Goh,K. Y.,Chong,D. Y. R.,Kapoor,R.,&Sun,A. Y. S.(1992). Thermal characterization of a thermally enhanced qfn package. Febs Letters,314(1):67-71.
[17]Li,X. P.,Olle Bjrkman,Shih,C.,Grossman,A. R.,&Niyogi,K. K.(2000). A pigment-binding protein essential for regulation of photosynthetic light harvesting. Nature, 403(6768):391-395.
[18] Horton,P.,Ruban,A. V.,&Walters,R. G.(1996).Regulation of light harvesting in green plants. Annual Review of Plant Physiology&Plant Molecular Biology,47(1):655-684.
[19]Li,Z.,Ahn,T. K.,Avenson,T. J.,Ballottari,M.,Cruz,J. A.,&Kramer,D. M.,et al.(2009). Lutein accumulation in the absence of zeaxanthin restores nonphotochemical quenching in the arabidopsis thaliana npq1 mutant. The Plant Cell Online,21(6):1,798-1,812.
[20]Li,X. P.,Gilmore,A. M.,Caffarri,S.,Bassi,R.,Golan,T.,&Kramer,D.,et al.(2004). Regulation of photosynthetic light harvesting involves intrathylakoid lumen ph sensing by the psbs protein. Journal of Biological Chemistry,279(22):22,866-22,874.
[21]Fariduddin,Q.,Hayat,S.,&Ahmad,A.(2003). Salicylic acid influences net photosynthetic rate,carboxylation efficiency,nitrate reductase activity,and seed yield inbrassica juncea. Photosynthetica(Prague),41(2):281-284.
[22]Zhang,M.,&Yang,X. E.(2014). Psbsexpression analysis in two ecotypes ofsedum alfrediiand the role ofsapsbsin cd tolerance. Russian Journal of Plant Physiology,61(4):427-433.
[23]Krah,N. M.,&Logan,B. A.(2010). Loss of PSBs expression reduces vegetative growth,reproductive output,and light-limited,but not light-saturated,photosynthesis in arabidopsis thaliana(brassicaceae)grown in temperate light environments.American Journal of Botany,97(4):644-649.
[24]高志民,于小清,郑波,等.毛竹PsbS基因的克隆及其原核表达特征研究[J].热带亚热带植物学报,2011,19(6):513-518.GAO Zhi-min,YU Xiao-qing,ZHANG Bo,et al.(2011).Cloning of PsbS gene from moso bamboo(Phyllostachys edulis)and its characterization of prokaryotic expression[J]. Journal of Tropical and Subtropical Botany,19(6):513-518.(in Chinese)
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