超高效液相色谱-四级杆-飞行时间质谱定性分析油炸马钱子中化学成分
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摘要
目的应用超高效液相色谱-四级杆串联飞行时间质谱法(UPLC-Q-TOF-MS)对油炸马钱子提取物的主要化学成分进行定性研究。方法采用Agilent SB-C_(18)(2. 1 mm×100 mm,1. 8μm)色谱柱,以0. 1%甲酸水(A)-乙腈(B)溶液为流动相,梯度洗脱,流速为0. 2 m L·min~(-1),柱温为35℃;质谱分析采用信息关联模式(IDA)正、负离子分别采集。结果本实验共鉴定出油炸马钱子提取物中31个化学成分,其中有8个为在马钱子中首次报道,并在正、负离子模式下发现4个新化合物;主要成分包括生物碱、有机酸、糖苷等。结论该方法精确、可靠、高效,适用于油炸马钱子成分的快速鉴定,为马钱子炮制品的开发利用以及阐明其药效物质基础提供参考。
OBJECTIVE To identify the main chemical constituents in the extract of deep fried strychnas by UPLC-Q-TOF-MS.METHODS Agilent SB-C_(18)( 2. 1 mm × 100 mm,1. 8 μm) column was used. Gradient elution was conducted with mobile phase consisting of 0. 1% formic acid solution( A) and acetonitrile( B) at a flow rate of 0. 2 m L·min~(-1). The column temperature was maitained at 35 ℃. MS analysis was based on information associated mode( IDA) that positive and negative ions were respectively collected.RESULTS A total of 31 compounds were identified in deep fried strychnas,of which eight had not been reported as Semen Strychni,and four new compounds were found in the positive and negative ion mode. The main chemical constituents included alkaloids,organic acids,glycosides,etc. CONCLUSION The method is accurate,reliable,and efficient,and is suitable for rapid identification of the ingredients in deep fried strychnas,which provides a reference for the development and utilization of processed products of Semen Strychni and clarification of its efficacy and material basis.
OBJECTIVE To identify the main chemical constituents in the extract of deep fried strychnas by UPLC-Q-TOF-MS.METHODS Agilent SB-C_(18)( 2. 1 mm × 100 mm,1. 8 μm) column was used. Gradient elution was conducted with mobile phase consisting of 0. 1% formic acid solution( A) and acetonitrile( B) at a flow rate of 0. 2 m L·min~(-1). The column temperature was maitained at 35 ℃. MS analysis was based on information associated mode( IDA) that positive and negative ions were respectively collected.RESULTS A total of 31 compounds were identified in deep fried strychnas,of which eight had not been reported as Semen Strychni,and four new compounds were found in the positive and negative ion mode. The main chemical constituents included alkaloids,organic acids,glycosides,etc. CONCLUSION The method is accurate,reliable,and efficient,and is suitable for rapid identification of the ingredients in deep fried strychnas,which provides a reference for the development and utilization of processed products of Semen Strychni and clarification of its efficacy and material basis.
引文
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[12] CHATTERJEE I,CHAKRAVARTY A K,GOMES A,et al. Antisnake venom activity of ethanolic seed extract of Strychnos nux vomica Linn[J]. Indian J Exp Biol,2004,42(5):468-475.
[13] JONVILLE M C,DIVE G,ANGENOT L,et al. Dimericbisindole alkaloids from the stem bark of Strychnosnux-vomica L[J].Phytochemistry,2013,87(3):157-163.
[14] ZHAO H,YAN Y,ZHOU L S,et al. Identification of chemical constituents in Magnoliae Officinalis Cortex by UFLC-Triple TOFMS/MS[J]. Chin Pharm J(中国药学杂志),2017,52(20):1824-1831.
[15] LIU J,ZHANG H Z. Research survey of attenuation synercy of Nux Vomica[J]. J Pract Tradit Chin Int Med(实用中医内科杂志),2015,29(6):159-163.
[16] LV S W,MA W B,XIAO H B,et al. Research progress on toxic mechanism of Strychnus Strychni and its combination with licorice in reducing toxicity[J]. J Hebei Tradit Chin Med Pharmacol(河北中医药学报),2018,33(2):59-64.
[2] GUO Y Y,MA W B,XIAN H B,et al. Changes of toxic substance in different phase of compatibility decoction Strychni Semen and Glycyrrhizae Radix et Rhizoma based on UPLC-MS technique[J]. Chin Tradit Herb Drugs(中草药),2017,48(23):4880-4884.
[3] YANG S L,YANG Y. A study on the attenuation method for toxicity of Maqianzi[J]. Clin Res Chin Med(中医临床研究),2017,9(1):43-44.
[4] WANG M Z,QI W Q. Effect of processing techniques on stryehnine content in Nux Vomica with different origins[J]. J Tradit Chin Med(中医学报),2017,32(7):1236-1238.
[5] WANG W. Study on the quality of the processing of Semen Strychni[J]. Inner Mongol J Tradit Chin Med(内蒙古中医药),2017,36(3):98-99.
[6] WU F,LIA R,GUO J W. Processing methods of historical development of Semen Strychni[J]. Pharm Today(今日药学),2017,27(5):355-357.
[7] LIU Y J,WU B Y,XUY,et al. Herbal textual research and processing history for Maqianzi[J]. Pharm Clin Chin Mater Med(中药与临床),2014,5(2):90-92.
[8] WANG D D,LI J S,CAI B C. Content change research of strychnine N-oxide and brucine N-oxide in maqianzi processiig drugs circa[J]. Chin Arch Tradit Chin Med(中华中医药学刊),2009,27(2):435-436.
[9] GONG Q F,ZHOU D G,ZHANG D F,et al. Experimental study on acute toxicity of Semen Strychni and its processed products[J]. Coll Tradit Chin Med(江西中医学院学报),2007,19(3):47-48.
[10] WU X J,MA F S,ZHEN G L,et al. Toxicity research progress on indole alkaloids of Strychni Semen[J]. Pharmacol Clin Chin Mater Med(中药药理与临床),2016,32(6):231-235.
[11] SUN L R,YUAN C H,LI X W,et al. Alkaloid constituents from frying seeds of Struchnosnux-vomica[J]. J Honghe Univ(红河学院学报),2011,9(4):5-8.
[12] CHATTERJEE I,CHAKRAVARTY A K,GOMES A,et al. Antisnake venom activity of ethanolic seed extract of Strychnos nux vomica Linn[J]. Indian J Exp Biol,2004,42(5):468-475.
[13] JONVILLE M C,DIVE G,ANGENOT L,et al. Dimericbisindole alkaloids from the stem bark of Strychnosnux-vomica L[J].Phytochemistry,2013,87(3):157-163.
[14] ZHAO H,YAN Y,ZHOU L S,et al. Identification of chemical constituents in Magnoliae Officinalis Cortex by UFLC-Triple TOFMS/MS[J]. Chin Pharm J(中国药学杂志),2017,52(20):1824-1831.
[15] LIU J,ZHANG H Z. Research survey of attenuation synercy of Nux Vomica[J]. J Pract Tradit Chin Int Med(实用中医内科杂志),2015,29(6):159-163.
[16] LV S W,MA W B,XIAO H B,et al. Research progress on toxic mechanism of Strychnus Strychni and its combination with licorice in reducing toxicity[J]. J Hebei Tradit Chin Med Pharmacol(河北中医药学报),2018,33(2):59-64.
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