一株嗜盐菌的分离鉴定及其降解甲基绿的实验
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摘要
从舟山双峰盐场的盐田中分离筛选得到一株可降解碱性染料甲基绿细菌Salinivibrio kushneri DD-1。研究了该菌株最佳的生长盐浓度以及在不同NaCl浓度、甲基绿浓度、pH值以及O_2浓度对该菌株降解甲基绿的影响,并对其降解产物进行分析。结果表明:该菌株为一株嗜盐菌,最佳生长盐浓度为50 g/L。脱色实验中,Salinivibrio kushneri DD-1在ρ(NaCl)为50 g/L时脱色率最高,达到95%;对低浓度甲基绿(<25 mg/L)的脱色效果较好;脱色率随pH升高而增大,该菌株在碱性条件下对甲基绿的脱色效果更佳; Salinivibrio kushneri DD-1在厌氧条件下无法降解甲基绿,随着O_2浓度升高,脱色效果逐渐增强。甲基绿降解产物主要为4-(N,N-二甲基氨基)-4'-(N',N'-二甲基氨基)二苯甲酮、4-(N-乙基-N,N-二甲基氨基)-4'-(N',N'-二甲基氨基)二苯甲酮、[4-(N-乙基-N,N-二甲基氨基)][4'-(N',N'-二甲基氨基)][4″(N″,N″-二甲基氨基)]三苯基甲醇。
A strain identified as Salinivibrio kushneri DD-1,was isolated from a salina of Zhoushan Shuangfeng Saltern and observed to decolor alkaline dye methyl green. This paper studied the optimum growth of DD-1 with different concentration of NaCl,and its decolorization efficiency on methyl green with different NaCl concentration,methyl green concentration,pH value and oxygen concentration. The results showed that DD-1 was a halophile and had the fastest growth rate with 50 g/L of NaCl. Decolorization efficiency was the highest and reached 95% with 50 g/L of NaCl. The decolorization efficiency was higher with lower concentration of methyl green( <25 mg/L) and higher pH value( especially in alkaline condition),and with higher oxygen concentration. Methyl green cannot be decolored in anaerobic condition. The main degradation production included4-( N,N-dimethylamino)-4'-( N', N'-dimethylamino) benzophenone, 4-( N-Ethyl-N, N-dimethylamino)-4'-( N', N'-dimethylamino) benzophenone and [4-( N-Ethyl-N,N-dimethylamino) ] [4'-( N',N'-dimethylamino) ] [4″-( N″,N″-dimethylamino) ] triphenylmethanol.
A strain identified as Salinivibrio kushneri DD-1,was isolated from a salina of Zhoushan Shuangfeng Saltern and observed to decolor alkaline dye methyl green. This paper studied the optimum growth of DD-1 with different concentration of NaCl,and its decolorization efficiency on methyl green with different NaCl concentration,methyl green concentration,pH value and oxygen concentration. The results showed that DD-1 was a halophile and had the fastest growth rate with 50 g/L of NaCl. Decolorization efficiency was the highest and reached 95% with 50 g/L of NaCl. The decolorization efficiency was higher with lower concentration of methyl green( <25 mg/L) and higher pH value( especially in alkaline condition),and with higher oxygen concentration. Methyl green cannot be decolored in anaerobic condition. The main degradation production included4-( N,N-dimethylamino)-4'-( N', N'-dimethylamino) benzophenone, 4-( N-Ethyl-N, N-dimethylamino)-4'-( N', N'-dimethylamino) benzophenone and [4-( N-Ethyl-N,N-dimethylamino) ] [4'-( N',N'-dimethylamino) ] [4″-( N″,N″-dimethylamino) ] triphenylmethanol.
引文
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[20]江莹,蔡亚君.细菌染料脱色机理研究进展[J].广州化工,2015,43(17):4-6.
[2]谯建军.三苯甲烷类染料废水微生物降解研究[D].西安:西安理工大学,2005.
[3] He Z B. Characterization of dyeing effluents and their treatment techniques[J]. Dyeing&Finishing,2007,17:41-44.
[4] Inoue T,Kikuchi K,Hirose K,et al. Small molecule-based laser inactivation of inositol 1,4,5-trisphosphate receptor[J]. Chemistry&Biology,2001,8(1):9-15.
[5] Kiadehi M,Amoozegar M A,et al. Exploring the potential of halophilic archaea for the decolorization of azo dyes[J]. Water Science&Technology,2018,77(6):1602-1611.
[6] Madureira K M,Gomes V. Total and differential leukocyte counts in the milk of healthy goats,using methyl green-pyronin stain and cytocentrifugation[J]. Arquivos Do Instituto Biológico,2010,77(2):343-347.
[7] Cho B P,Yang T L,Blankenship L R,et al. Synthesis and characterization of N-demethylated metabolites of malachite green and leucomalachite green[J]. Chemical Research in Toxicology,2003,16(3):285-294.
[8]张晓华,赵保卫,任学昌,等.一种三苯甲烷类染料脱色菌液的制备及其脱色性能[J].中国给水排水,2016(9):98-102.
[9] Jp S. Biological degradation of reactive dyes by using bacteria isolated from dye effluent contaminated soil[J]. Middle East Journal of Scientific Research,2013,17(12):1695-1700.
[10] Saratale R G, Saratale G D, Chang J S, et al. Bacterial decolorization and degradation of azo dyes:a review[J]. Journal of the Taiwan Institute of Chemical Engineers,2011,42(1):138-157.
[11]杨清香,贾振杰,杨敏.微生物染料脱色研究进展[J].微生物学通报,2006,33(4):144-148.
[12]仲玲玲,孙东豪,苏小军,等.酸性/碱性染料的生物降解分析[J].苏州大学学报(工科版),2012,32(5):70-75.
[13] Zhou Y,Lu H,Wang J,et al. Catalytic performance of quinone and graphene-modified polyurethane foam on the decolorization of azo dye Acid Red 18 by Shewanella sp. RQs-106[J]. Journal of Hazardous Materials,2018,356:82-90.
[14] Asad S,Amoozegar M A,Pourbabaee A A,et al. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria[J]. Bioresource Technology,2007,98(11):2082-2088.
[15] Leigh J A,Albers S V,Atomi H,et al. Model organisms for genetics in the domain Archaea:methanogens, halophiles,Thermococcales, and Sulfolobales[M]. Oxford:FEMS Microbiology Reviews,2011.
[16]安立超,严学亿,胡磊,等.嗜盐菌的特性与高盐废水生物处理的进展[J].环境污染与防治,2002,24(5):293-296.
[17]戴日成,张统.印染废水水质特征及处理技术综述[J].给水排水,2000,26(10):33-37.
[18] Wang Y C, Yu R C, Chou C C. Growth and survival of bifidobacteria and lactic acid bacteria during the fermentation and storage of cultured soymilk drinks[J]. Food Microbiology,2002,19(5):501-508.
[19]丁凤友.几种分散染料的生物降解特性研究[D].上海:东华大学,2016.
[20]江莹,蔡亚君.细菌染料脱色机理研究进展[J].广州化工,2015,43(17):4-6.