8个马大杂种相思无性系组培快繁试验初报
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摘要
通过对马大杂种相思8个无性系号(10#、73#、LK1、LK2、LK3、SK1、SK2、SK3)的组培快繁进行试验,从影响外植体诱导、增殖、瓶内生根、驯化与移栽等因素进行研究。结果表明:马大杂种相思理想的外植体材料是当年生枝条第3～5个腋芽茎段;外植体两种消毒方法(70%酒精+0.1%升汞)和(2%次氯酸钠+0.1%升汞)对消毒效果没多大区别;两种消毒剂同时使用时务必掌握好消毒时间,0.1%升汞6min是最合适的,诱导率达58.2%;增殖效果与6 BA浓度有关,固定NAA为0.1mg·L-1,6BA最佳浓度以0.4mg/L和0.6mg/L为较适范围,同时与基本培养基有关,其中MS培养基对促进芽分化效果最显著,培养4个月时已进入旺盛增殖期,6个月的倍率为3.87;瓶内生根培养以V6:1/2MS+IBA1.2 mg/L+NAA0.2 mg/L和V13:1/2MS+NAA 1.2 mg/L+IBA0.2 mg/L的生根效果最好,生根率都达96%,生根数平均达3.6条以上;移栽基质选择以黄心土:沙为1:1效果最合适的;8个号移栽的平均成活率有一定的差异,超过80%是10#(87.3%)、73#(83.5%)、SK1(87.8%),其它号为70%左右;制定了马大相思从增殖倍率、生根率及瓶内生根生产能力的综合评价体系,初步认定SK1、10#、73#3个号为可形成瓶内生根生产能力并规模工厂化生产苗木的品号。
We test the histological propagation of 8 asexual numbers(10 #,73 #,LK1,LK2,LK3,SK1,SK2,SK3)of the Acacia Mongrel. The effects of induction,proliferation, rooting,domestication and transplanting were studied. The results showed that the ideal exophytic material of Acacia was the 3 rd to 5 th axillary shoots of the same year. The two disinfection methods for implants(70 % alcohol + 0.1 % Mercury)and(2 % sodium hypochlorite + 0.1 % Mercury)have little difference in the disinfection effect,and the success rate is more than 30 %. When two disinfectants are used at the same time,must master the disinfection time. 0.1 % litres of mercury for 6 minutes is the most appropriate,with an induction rate of 58.2 %;The proliferation effect is related to the 6 BA concentration. The fixed NAA is 0.1 mg·L-1. The best concentration of 6 BA is 0.4 mg/L 和 0.6 mg/L,and it is related to the basic medium. The MS medium has the most significant effect on promoting bud differentiation. At the time of cultivation for 4 months,it has entered a period of vigorous proliferation,and the doubling rate for 6 months is 3.87;The rooting effect of V6:1/2 MS + IBA1.2 mg/L + NAA 0.2 mg/L and V13:1/2 MS + NAA 1.2 mg/L + IBA 0.2 mg/L was best in bottle rooting culture. The rooting rate reached 96 %, and the average number of roots reached 3.6 or more;Transplantation matrix selection with LOESS soil:sand is 1:1 effect is appropriate;The average survival rate of transplanting is 8 with certain differences,with more than 80 % being 10 #(87.3 %),73 #(83.5%),SK1(87.8 %),and other numbers being about 70 %;A comprehensive evaluation system of the multiplication rate,rooting rate,and rooting capacity of Acacia matha was established,and SK1,10 #,73 # 3 numbers were initially identified as the numbers that could form the rooting capacity in the bottle and scale the factory production of seedlings.
We test the histological propagation of 8 asexual numbers(10 #,73 #,LK1,LK2,LK3,SK1,SK2,SK3)of the Acacia Mongrel. The effects of induction,proliferation, rooting,domestication and transplanting were studied. The results showed that the ideal exophytic material of Acacia was the 3 rd to 5 th axillary shoots of the same year. The two disinfection methods for implants(70 % alcohol + 0.1 % Mercury)and(2 % sodium hypochlorite + 0.1 % Mercury)have little difference in the disinfection effect,and the success rate is more than 30 %. When two disinfectants are used at the same time,must master the disinfection time. 0.1 % litres of mercury for 6 minutes is the most appropriate,with an induction rate of 58.2 %;The proliferation effect is related to the 6 BA concentration. The fixed NAA is 0.1 mg·L-1. The best concentration of 6 BA is 0.4 mg/L 和 0.6 mg/L,and it is related to the basic medium. The MS medium has the most significant effect on promoting bud differentiation. At the time of cultivation for 4 months,it has entered a period of vigorous proliferation,and the doubling rate for 6 months is 3.87;The rooting effect of V6:1/2 MS + IBA1.2 mg/L + NAA 0.2 mg/L and V13:1/2 MS + NAA 1.2 mg/L + IBA 0.2 mg/L was best in bottle rooting culture. The rooting rate reached 96 %, and the average number of roots reached 3.6 or more;Transplantation matrix selection with LOESS soil:sand is 1:1 effect is appropriate;The average survival rate of transplanting is 8 with certain differences,with more than 80 % being 10 #(87.3 %),73 #(83.5%),SK1(87.8 %),and other numbers being about 70 %;A comprehensive evaluation system of the multiplication rate,rooting rate,and rooting capacity of Acacia matha was established,and SK1,10 #,73 # 3 numbers were initially identified as the numbers that could form the rooting capacity in the bottle and scale the factory production of seedlings.
引文
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[4]熊建勇,宗亦臣,常金财,等.马大相思茎段的组织培养[J].林业实用技术,2011,9:31-32.
[5]高新一,王玉英.植物无性繁殖实用技术[M].北京:金盾出版社,2003:362-368.
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[7]林来水.黑木相思优良无性系离体培养与快速繁殖技术研究[D].福州:福建农林大学,2008.
[8]谢寅峰,张志敏,尚旭岚,等.青钱柳茎段腋芽萌发和丛生芽增殖[J].林业科学,2011,47(1):50-55.
[2]陈诚,蒲俊文,姚胜,等.速生阔叶马大相思制浆性能的研究[J].造纸科学与技术,2009,28(4):1-3.
[3]周丽华,张华通,刘颂颂,等.相思杂种:莞屏1号离体快速繁殖的研究[J].广东林业科技,1996,12(3):26-29.
[4]熊建勇,宗亦臣,常金财,等.马大相思茎段的组织培养[J].林业实用技术,2011,9:31-32.
[5]高新一,王玉英.植物无性繁殖实用技术[M].北京:金盾出版社,2003:362-368.
[6]谭文澄.观赏植物的组织培养技术[M].北京:中国林业出版社,2001:100-105.
[7]林来水.黑木相思优良无性系离体培养与快速繁殖技术研究[D].福州:福建农林大学,2008.
[8]谢寅峰,张志敏,尚旭岚,等.青钱柳茎段腋芽萌发和丛生芽增殖[J].林业科学,2011,47(1):50-55.