Rab27B和HPV16/18蛋白质在宫颈鳞癌表达的意义
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摘要
目的通过免疫组织化学实验探讨Rab27B和HPV16/18蛋白质在宫颈鳞癌组织中表达的意义。方法收集慢性宫颈炎组织和宫颈鳞癌组织连续石蜡切片,采用免疫组织化学实验检测慢性宫颈炎组织和宫颈鳞癌组织中HPV16/18和Rab27B蛋白质的表达水平。通过统计学分析分别研究HPV16/18和Rab27B蛋白质在宫颈鳞癌组织的表达意义。结果 HPV16/18在慢性宫颈炎组织中感染率为13. 5%,而在宫颈鳞癌组织中为46. 2%,差异有统计学意义(P<0. 05)。Rab27B蛋白质表达水平在慢性宫颈炎组织中,中、强阳性表达为32. 4%。在宫颈鳞癌组织中,中、强阳性表达为63. 3%。两者比较具有统计学意义(P<0. 05)。宫颈鳞癌组织中Rab27B表达水平高,HPV16/18表达水平也高;慢性宫颈炎组织中Rab27B表达水平较低,HPV16/18表达水平也低(P<0. 05)。结论宫颈鳞癌组织中Rab27B蛋白质的高表达结合HPV16/18蛋白质的表达,可能和宫颈鳞癌的发生和发展相关。
Objective To explore expression significance of Rab27 B and HPV16/18 protein in tissues of squamous carcinoma of the cervix by immunohistochemistry experiment. Methods Collected the continuous paraffin sections of chronic cervicitis and squamous carcinoma of the cervix tissues. The expression levels of HPV16/18 and Rab27 B protein in chronic cervicitis and cervical cancer tissues was detected by immunohistochemistry experiment. The expression significance of HPV16/18 and Rab27 B proteins in cervical cancer tissues was studied by statistical analysis,respectively. Results HPV16/18 infection rate in tissues of chronic cervicitis is 13. 5%,whereas HPV 16/18 infection rate in tissues of cervical cancer is 46. 2%,the difference is statistically significant( P <0. 05). In addition,Rab27 B protein expression level accounted for 32. 4% of the moderate positive and strong positive rate in chronic cervicitis tissues,moreover,the moderate positive and strong positive rate is 63. 3% in tissues of cervical cancer,the difference is statistically significant( P<0. 05). Furthermore,Rab27 B protein is highly expressed so as HPV16/18 protein expression level in tissues of cervical cancer,whereas,Rab27 B protein expressed lowly so as HPV16/18 protein in chronic cervicitis tissues( P<0. 05). Conclusion The higher expression of Rab27 B protein in cervical cancer tissues may be related to tumorigenesis. The expression of Rab27 B protein in cervical cancer tissues combined with the expression of HPV16/18 protein may contribute to the tumorigenesis and development of cervical squamous cell carcinoma.
Objective To explore expression significance of Rab27 B and HPV16/18 protein in tissues of squamous carcinoma of the cervix by immunohistochemistry experiment. Methods Collected the continuous paraffin sections of chronic cervicitis and squamous carcinoma of the cervix tissues. The expression levels of HPV16/18 and Rab27 B protein in chronic cervicitis and cervical cancer tissues was detected by immunohistochemistry experiment. The expression significance of HPV16/18 and Rab27 B proteins in cervical cancer tissues was studied by statistical analysis,respectively. Results HPV16/18 infection rate in tissues of chronic cervicitis is 13. 5%,whereas HPV 16/18 infection rate in tissues of cervical cancer is 46. 2%,the difference is statistically significant( P <0. 05). In addition,Rab27 B protein expression level accounted for 32. 4% of the moderate positive and strong positive rate in chronic cervicitis tissues,moreover,the moderate positive and strong positive rate is 63. 3% in tissues of cervical cancer,the difference is statistically significant( P<0. 05). Furthermore,Rab27 B protein is highly expressed so as HPV16/18 protein expression level in tissues of cervical cancer,whereas,Rab27 B protein expressed lowly so as HPV16/18 protein in chronic cervicitis tissues( P<0. 05). Conclusion The higher expression of Rab27 B protein in cervical cancer tissues may be related to tumorigenesis. The expression of Rab27 B protein in cervical cancer tissues combined with the expression of HPV16/18 protein may contribute to the tumorigenesis and development of cervical squamous cell carcinoma.
引文
[1]潘贞贞,潘欢,朱君玲,等.宫颈癌组织中HPV16 L1蛋白质的表达及意义[J].石河子大学学报(自然科学版),2017,35(4):458-461.Pan Z Z,Pan H,Zhu J L,et al.The expression and significance of HPV16 L1 protein in cervical cancer tissues[J].Journal of Shihezi University(Natural Science),2017,35(4):458-461.
[2]者湘漪,朱君玲,姜心雨,等.新疆维吾尔族宫颈癌组织人乳头瘤病毒16型E6、E7基因变异分析[J].石河子大学学报(自然科学版),2017,35(6):738-742.Zhe X Y,Zhu J L,Jiang X Y,et al.The study of gene variant analysis of HPV16 E6 and E7 gene in Uygur women of Xinjiang[J].Journal of Shihezi University(Natural Science),2017,35(6):738-742.
[3]Iancu I V,Anton G,Botezatu A,et al.LINC01101 and LINC00277 expression levels as novel factors in HPV-induced cervical neoplasia[J].J Cell Mol Med,2017,21(12):3787-3794.
[4]Masliahplanchon J,Garinet S,Pasmant E.RAS-MAPKpathway epigenetic activation in cancer:miRNAs in action.[J].Oncotarget,2016,7(25):38892-38907.
[5]王晓娟,郭岩,肖文华.Rab27与肿瘤的研究进展[J].中国现代医生,2015,53(34):157-160.Wang X J,Guo Y,Xiao W H.Progess in research on Rab27 and tumor[J].Chinese Modern Doctor,2015,53(34):157-160.
[6]Tolmachova T,Abrink M,Futter C E,et al.Rab27b regulates number and secretion of platelet dense granules[J].Proceedings of the National Academy of Sciences,2007,104(14):5872-5877.
[7]Hendrix A,Maynard D,Pauwels P,et al.Effect of the secretory small GTPase Rab27B on breast cancer growth,invasion,and metastasis[J].J Natl Cancer Inst,2010,102(12):866-880.
[8]Shahbazi S,Bolhassani A.Comparison of six cell penetrating peptides with different properties for in vitro and in vivo delivery of HPV16 E7 antigen in therapeutic vaccines[J].Int Immunopharmacol,2018,62:170-180.
[9]Senapati R,Nayak B,Kar S K,et al.HPV genotypes coinfections associated with cervical carcinoma:Special focus on phylogenetically related and non-vaccine targeted genotypes[J].Plos One,2017,12(11):96.
[10]Hendrix A,Lambein K,Westbroek W,et al.An immunohistochemical analysis of Rab27B distribution in fetal and adult tissue[J].The International Journal of Developmental Biology,2012,56(5):363-368.
[11]Hendrix A,Maynard D,Pauwels P,et al.The Secretory small GTPase Rab27B regulates invasive tumor growth and metastasis through extracellular heat shock protein90α.[J].Cancer Research,2009,69(24 Supplement):6144-6144.
[12]Wang H,Wang Y,Bao Z,et al.Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion[J].Oncology Reports,2015,34(3):1503.
[13]Dong W W,Mou Q,Chen J,et al.Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma[J].World Journal of Gastroenterology,2012,18(15):1806-1813.
[14]Li J,Jin Q,Huang F,et al.Effects of Rab27A and Rab27B on invasion,proliferation,apoptosis,and chemoresistance in human pancreatic cancer cells[J].Pancreas,2017,46(9):1173-1179.
[15]Wang W,Ni Q,Wang H,et al.Prognostic value of Rab27B nuclear expression in gastrointestinal stromal tumors[J].Disease Markers,2014,2014(10):942181.
[16]Koh H M,Song D H.Prognostic role of Rab27A and Rab27B expression in patients with non-small patients with non-small cell lung carcinoma[J].Thorac Cancer,2019,10(2):143-149.
[17]Honegger A,Schilling D,Bastian S,et al.Dependence of intracellular and exosomal micro RNAs on viral E6/E7oncogene expression in HPV-positive tumor cells[J].PLo S Pathog,2015,11(3):e1004712.
[18]Ostrowski M,Carmo N B,Krumeich S,et al.Rab27a and Rab27b control different steps of the exosome secretion pathway.[J].Nature Cell Biology,2010,12(1):19-30.
[19]吴若愚,贾翔,曹忆梦,等.外泌体在常见妇科恶性肿瘤诊治中的研究进展[J].国际妇产科学杂志,2017,44(3):276-279.Wu R Y,Jia X,Cao Y M,et al.Research progress of exosomes in the diagnosis and treatment of common gynecologycal malignancies[J].International Journal of Gynecology and Obstetrics,2017,44(3):276-279.
[2]者湘漪,朱君玲,姜心雨,等.新疆维吾尔族宫颈癌组织人乳头瘤病毒16型E6、E7基因变异分析[J].石河子大学学报(自然科学版),2017,35(6):738-742.Zhe X Y,Zhu J L,Jiang X Y,et al.The study of gene variant analysis of HPV16 E6 and E7 gene in Uygur women of Xinjiang[J].Journal of Shihezi University(Natural Science),2017,35(6):738-742.
[3]Iancu I V,Anton G,Botezatu A,et al.LINC01101 and LINC00277 expression levels as novel factors in HPV-induced cervical neoplasia[J].J Cell Mol Med,2017,21(12):3787-3794.
[4]Masliahplanchon J,Garinet S,Pasmant E.RAS-MAPKpathway epigenetic activation in cancer:miRNAs in action.[J].Oncotarget,2016,7(25):38892-38907.
[5]王晓娟,郭岩,肖文华.Rab27与肿瘤的研究进展[J].中国现代医生,2015,53(34):157-160.Wang X J,Guo Y,Xiao W H.Progess in research on Rab27 and tumor[J].Chinese Modern Doctor,2015,53(34):157-160.
[6]Tolmachova T,Abrink M,Futter C E,et al.Rab27b regulates number and secretion of platelet dense granules[J].Proceedings of the National Academy of Sciences,2007,104(14):5872-5877.
[7]Hendrix A,Maynard D,Pauwels P,et al.Effect of the secretory small GTPase Rab27B on breast cancer growth,invasion,and metastasis[J].J Natl Cancer Inst,2010,102(12):866-880.
[8]Shahbazi S,Bolhassani A.Comparison of six cell penetrating peptides with different properties for in vitro and in vivo delivery of HPV16 E7 antigen in therapeutic vaccines[J].Int Immunopharmacol,2018,62:170-180.
[9]Senapati R,Nayak B,Kar S K,et al.HPV genotypes coinfections associated with cervical carcinoma:Special focus on phylogenetically related and non-vaccine targeted genotypes[J].Plos One,2017,12(11):96.
[10]Hendrix A,Lambein K,Westbroek W,et al.An immunohistochemical analysis of Rab27B distribution in fetal and adult tissue[J].The International Journal of Developmental Biology,2012,56(5):363-368.
[11]Hendrix A,Maynard D,Pauwels P,et al.The Secretory small GTPase Rab27B regulates invasive tumor growth and metastasis through extracellular heat shock protein90α.[J].Cancer Research,2009,69(24 Supplement):6144-6144.
[12]Wang H,Wang Y,Bao Z,et al.Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion[J].Oncology Reports,2015,34(3):1503.
[13]Dong W W,Mou Q,Chen J,et al.Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma[J].World Journal of Gastroenterology,2012,18(15):1806-1813.
[14]Li J,Jin Q,Huang F,et al.Effects of Rab27A and Rab27B on invasion,proliferation,apoptosis,and chemoresistance in human pancreatic cancer cells[J].Pancreas,2017,46(9):1173-1179.
[15]Wang W,Ni Q,Wang H,et al.Prognostic value of Rab27B nuclear expression in gastrointestinal stromal tumors[J].Disease Markers,2014,2014(10):942181.
[16]Koh H M,Song D H.Prognostic role of Rab27A and Rab27B expression in patients with non-small patients with non-small cell lung carcinoma[J].Thorac Cancer,2019,10(2):143-149.
[17]Honegger A,Schilling D,Bastian S,et al.Dependence of intracellular and exosomal micro RNAs on viral E6/E7oncogene expression in HPV-positive tumor cells[J].PLo S Pathog,2015,11(3):e1004712.
[18]Ostrowski M,Carmo N B,Krumeich S,et al.Rab27a and Rab27b control different steps of the exosome secretion pathway.[J].Nature Cell Biology,2010,12(1):19-30.
[19]吴若愚,贾翔,曹忆梦,等.外泌体在常见妇科恶性肿瘤诊治中的研究进展[J].国际妇产科学杂志,2017,44(3):276-279.Wu R Y,Jia X,Cao Y M,et al.Research progress of exosomes in the diagnosis and treatment of common gynecologycal malignancies[J].International Journal of Gynecology and Obstetrics,2017,44(3):276-279.
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