干旱和复水对两种葡萄砧木叶片光合和叶绿素荧光特性的影响
|
摘要
采用盆栽称重控水法,干旱胁迫处理1103P和101-14M两种砧木,处理21 d后复水,分别测定干旱处理0、7、14、21 d及复水第7、14天,葡萄砧木叶片光合和叶绿素荧光参数。结果显示:干旱胁迫后,1103P和101-14M的净光合速率(Pn)均逐渐降低,101-14M的Pn降幅大于1103P,短时间干旱胁迫引起两种砧木Pn降低的主要因素是气孔限制,而长时间干旱胁迫后Pn降低主要是非气孔限制。随着干旱胁迫的持续,1103P和101-14M的初始荧光产量(Fo)呈增加趋势,但101-14M的增幅大于1103P,说明干旱胁迫后101-14M的光反应中心受损害程度大于1103P;复水后1103P和101-14M两种砧木的Pn值逐渐增加,复水第7天,二者分别为对照的83.20%和66.31%,复水第14天,分别为对照的107.30%和88.43%;复水后1103P和101-14M两种砧木的Fo值呈现逐渐降低趋势,复水后第7天,1103P和101-14M的Fo值为对照的102.95%和109.60%,复水后第14天,1103P和101-14M Fo为对照的101.56%和101.81%,说明复水后1103P和101-14M两种砧木受损的光合反应中心得到了修复,光合速率也逐渐恢复,1103P复水后恢复生长的能力高于101-14M。
In order to better understand the photosynthetic adaptability of two grape rootstocks,1103 P and 101-14 M,to drought stress,the effect of continuous drought stress and rewatering on the characteristics of photosynthesis and chlorophyll fluorescence were studied. The 1103 P and 101-14 M were growing in pots with 40% ～ 45% of soil relative water content( RWC) and at day 21,they were rewatered to raise the soil RWC between 70% ～ 75%.The plants growing in the soil with 70% ～ 75% RWC throughout the growing season were used as control. The parameters of photosynthesis and chlorophyll fluorescence were measured on the 0 th,7 th,14 th,and 21 st day after drought treatment and on the 7 th and 14 th day after rewatering. Results showed that continuous drought stress influenced the the net photosynthetic rate( Pn) of 101-14 M and 1103 P successively decreased in varying degrees,and the decrease of Pn of 101-14 M was greater than 1103 P. Stomatal limitation play dominant role in decline of photosynthesis in short-term drought stress,non-stomatal limitation was the main reason in decline of photosynthesis while long-term drought stress. As the drought stress continued,the minimal fluorescence( Fo) of 1103 P and 101-14 M increased,while the increase of 101-14 m was greater than 1103 P,which indicated that the degree of damage to the photosynthesis organ of 101-14 M was higher than 1103 P after drought stress. The Pn of 1103 P and 101-14 M leaves increased gradually after rewatering. At the day 7 after rewatering,1103 P and 101-14 M Pn gradually increased to 83.20% and 66.31% of the control. At the 14 thday after rewatering,Pn values of 1103 P and 101-14 M were at 107.30% and 88.43% compared to that of the control. The Fo of 1103 P and 101-14 M leaves decreased gradually after rewatering. At the 7 thday after rewatering,the Fo of 1103 P and 101-14 M decreased to 102.95%and 109.60% over the control,and the 14 thday after rewatering,the Fo of 1103 P and 101-14 M decreased to101.56% and 101.81% compared to the control. These results indicated that the damage to the photosynthesis organs could be repaired and reach the same level of Fo of the control after rewatering,and photosynthesis of 1103 P leaf had the stronger ability to recover from drought stress than that of 101-14 M once the stress was terminated.
In order to better understand the photosynthetic adaptability of two grape rootstocks,1103 P and 101-14 M,to drought stress,the effect of continuous drought stress and rewatering on the characteristics of photosynthesis and chlorophyll fluorescence were studied. The 1103 P and 101-14 M were growing in pots with 40% ～ 45% of soil relative water content( RWC) and at day 21,they were rewatered to raise the soil RWC between 70% ～ 75%.The plants growing in the soil with 70% ～ 75% RWC throughout the growing season were used as control. The parameters of photosynthesis and chlorophyll fluorescence were measured on the 0 th,7 th,14 th,and 21 st day after drought treatment and on the 7 th and 14 th day after rewatering. Results showed that continuous drought stress influenced the the net photosynthetic rate( Pn) of 101-14 M and 1103 P successively decreased in varying degrees,and the decrease of Pn of 101-14 M was greater than 1103 P. Stomatal limitation play dominant role in decline of photosynthesis in short-term drought stress,non-stomatal limitation was the main reason in decline of photosynthesis while long-term drought stress. As the drought stress continued,the minimal fluorescence( Fo) of 1103 P and 101-14 M increased,while the increase of 101-14 m was greater than 1103 P,which indicated that the degree of damage to the photosynthesis organ of 101-14 M was higher than 1103 P after drought stress. The Pn of 1103 P and 101-14 M leaves increased gradually after rewatering. At the day 7 after rewatering,1103 P and 101-14 M Pn gradually increased to 83.20% and 66.31% of the control. At the 14 thday after rewatering,Pn values of 1103 P and 101-14 M were at 107.30% and 88.43% compared to that of the control. The Fo of 1103 P and 101-14 M leaves decreased gradually after rewatering. At the 7 thday after rewatering,the Fo of 1103 P and 101-14 M decreased to 102.95%and 109.60% over the control,and the 14 thday after rewatering,the Fo of 1103 P and 101-14 M decreased to101.56% and 101.81% compared to the control. These results indicated that the damage to the photosynthesis organs could be repaired and reach the same level of Fo of the control after rewatering,and photosynthesis of 1103 P leaf had the stronger ability to recover from drought stress than that of 101-14 M once the stress was terminated.
引文
[1] Cai J L,Olli V,Yin H. China's water resources vulnerability:A spa-tio-temporal analysis during 2003–2013[J]. Journal of Cleaner Pro-duction,2016,23(3):1890-1902.
[2]王新源,赵学勇,李玉霖,等.环境因素对干旱半干旱区凋落物分解的影响研究进展[J].应用生态学报,2013,24(11):3300-3310.
[3]王新源,李玉霖,赵学勇,等.干旱半干旱区不同环境因素对土壤呼吸影响研究进展[J].生态学报,2012,32(15):4890-4901.
[4] Meggio B,Prinsi A S,Negri G,et al. Biochemical and physiologicalresponses of two grapevine rootstock genotypes to drought and salttreatments[J]. Australian Journal of Grape and Wine Research,2014(2):569-584.
[5] Farooq M,Wahid A,Kobayashi N,et al. Plant drought stress:Effects,mechanisms and management[J]. Sustainable Agriculture,2009,29(1):185-212.
[6] Anjum S A,Xie X Y,Wang L C,et al. Morphological,physiologicaland biochemical responses of plants to drought stress[J]. African Jour-nal of Agricultural Research,2011,16(90):2026-2032.
[7] Comic G. Drought stress inhibits photosynthesis by decreasing stomatalaperture not by affecting ATP synthesis[J]. Trends in Plant Science,2000,(5):187-188.
[8] Lawson T,Oxborough K,Morison J L,et al. The responses of guardand mesophyll cell photosynthesis to CO2,O2,light and water stressin a range of species are similar[J]. Journal of Experimental Botany,2003,54(388):1743-1752.
[9]王磊,胡楠,张彤,等.干旱和复水对大豆(Glycine max)叶片光合及叶绿素荧光的影响[J].生态学报,2007,(09):3630-3636.
[10] Chaves M M,Pereira J S,Mamco J. How plants cope with waterstress in the field[J]. Phomsynthesis and Growth,Ann Bot(Lond),2002,89(7):907-916.
[11] Liu X Y,Luo Y P. Present situation of study on after-effect of waterstress on crop growth[J]. Agricultural Reseach in the Arid Areas,2002,20(4):6-10.
[12]于文颖,纪瑞鹏,冯锐,等.不同程度干旱胁迫及复水对春玉米(丹玉39)茎流动态的影响[J].干旱地区农业研究,2016,34(02):163-170.
[13]李泽,谭晓风,卢锟,等.干旱胁迫对两种油桐幼苗生长、气体交换及叶绿素荧光参数的影响[J].生态学报,2017,37(05):1515-1524.
[14]卞凤娥,孙永江,牛彦杰,等.高温胁迫下根施褪黑素对葡萄叶片叶绿素荧光特性的影响[J].植物生理学报,2017,53(02):257-263.
[15]张强,杨平,张边江.叶绿素荧光技术在彩叶植物引种评价中的应用[J].分子植物育种,2017,15(03):1114-1120.
[16]刘世秋,张振文,惠竹梅,等.干旱胁迫对酿酒葡萄赤霞珠光合特性的影响[J].干旱地区农业研究,2008,(05):169-172.
[17]王振兴,陈丽,艾军,等.不同干旱胁迫对山葡萄的光合作用和光系统II活性的影响[J].植物生理学报,2014,50(08):1171-1176.
[18]胡宏远,王振平.干旱胁迫对赤霞珠葡萄叶片水分及叶绿素荧光参数的影响[J].干旱区资源与环境,2017,31(04):124-130.
[19] Burman U,Garg B.K.,Kathju S. Water relations,Photosynthesis andnitrogen metabolism of Indian mustard grown under salt and waterstress[J]. Journal of Plant Biology,2003,30(1):55-60.
[20] Sharma P N,Tripathi A,Bisht S S. Zinc requirement for stomatal o-pening in cauliflower[J]. Plant Physiology,1995,107:751-756.
[21]牛铁泉,田给林,薛仿正,等.半根及半根交替水分胁迫对苹果幼苗光合作用的影响[J].中国农业科学,2007,40(07):1463-1468.
[22]张继义,赵哈林.短期极端干旱事件干扰下退化沙质草地群落抵抗力稳定性的测度与比较[J].生态学报,2010,30(20):5456-5465.
[23]李金航,齐秀慧,徐程扬,等.黄栌幼苗叶片气体交换对干旱胁迫的短期响应[J].林业科学,2015,51(01):29-41.
[24] Medrano H,Escalona J M,Bota J,et al. Regulation of photosynthesisof C3plants in response to progressive drought:stomatal conductanceas a reference parameter[J]. Annals of botany,2002,89(7):895-905.
[25]杨文权,顾沐宇,寇建村,等.干旱及复水对小冠花光合及叶绿素荧光参数的影响[J].草地学报,2013,21(06):1130-1135.
[26]蒙祖庆,宋丰萍,刘振兴,等.干旱及复水对油菜苗期光合及叶绿素荧光特性的影响[J].中国油料作物学报,2012,34(01):40-47.
[27]袁军伟,李敏敏,刘长江,等.盐胁迫下马瑟兰/101-14嫁接苗和自根苗光合生理及叶绿素荧光参数的差异[J].西北农业学报,2016,25(05):758-762.
[28] Parida A K,das A B. Salt tolerance and salinity effects on plants:areview[J]. Ecotoxicology and Environmental Safety,2005,60(3):324-349.
[29]吴甘霖,段仁燕,王志高,等.干旱和复水对草莓叶片叶绿素荧光特性的影响[J].生态学报,2010,30(14):3941-3946.
[30] Bota J,Flexas J,Medrano H. Is photosynthesis limited by decreasedRubisco activity and Ru BP content unprogressive water stress[J].New Phytologist,2004,162(3):671-681.
[31] Schreiber U,Gademann R,Ralph P J. Assessment of photosyntheticperformance of prochloron in Lissoclinum patella in hospite by chloro-phyll fluorescence measurements[J]. Plant Cell and Physiology,1997,38(8):945-951.
[32] Massacci A,Nabiv S M,Pietrosanti L. Response of photosynthesis ap-paratus of cotton to the onset of drought stress under field conditionsby gas change analysis and chlorophyll fluorescence imaging[J].Plant Physiology and Biochemistry,2008,46(2):189-195.
[33] Santos M G,Ribeiro R V,Machado E C. Effects of drought stress onphotosynthetic parameters and leaf water potential of five commonbean genotypes under water deficit[J]. Biologia Plantarum,2009,53(2):229-236.
[34]赵丽英,邓西平,山仑.渗透胁迫对小麦幼苗叶绿素荧光参数的影响[J].应用生态学报,2005,16(07):1261-1264.
[35]厉广辉,万勇善,刘风珍,等.苗期干旱及复水条件下不同花生品种的光合特性[J].植物生态学报,2014,38(07):729-739.
[2]王新源,赵学勇,李玉霖,等.环境因素对干旱半干旱区凋落物分解的影响研究进展[J].应用生态学报,2013,24(11):3300-3310.
[3]王新源,李玉霖,赵学勇,等.干旱半干旱区不同环境因素对土壤呼吸影响研究进展[J].生态学报,2012,32(15):4890-4901.
[4] Meggio B,Prinsi A S,Negri G,et al. Biochemical and physiologicalresponses of two grapevine rootstock genotypes to drought and salttreatments[J]. Australian Journal of Grape and Wine Research,2014(2):569-584.
[5] Farooq M,Wahid A,Kobayashi N,et al. Plant drought stress:Effects,mechanisms and management[J]. Sustainable Agriculture,2009,29(1):185-212.
[6] Anjum S A,Xie X Y,Wang L C,et al. Morphological,physiologicaland biochemical responses of plants to drought stress[J]. African Jour-nal of Agricultural Research,2011,16(90):2026-2032.
[7] Comic G. Drought stress inhibits photosynthesis by decreasing stomatalaperture not by affecting ATP synthesis[J]. Trends in Plant Science,2000,(5):187-188.
[8] Lawson T,Oxborough K,Morison J L,et al. The responses of guardand mesophyll cell photosynthesis to CO2,O2,light and water stressin a range of species are similar[J]. Journal of Experimental Botany,2003,54(388):1743-1752.
[9]王磊,胡楠,张彤,等.干旱和复水对大豆(Glycine max)叶片光合及叶绿素荧光的影响[J].生态学报,2007,(09):3630-3636.
[10] Chaves M M,Pereira J S,Mamco J. How plants cope with waterstress in the field[J]. Phomsynthesis and Growth,Ann Bot(Lond),2002,89(7):907-916.
[11] Liu X Y,Luo Y P. Present situation of study on after-effect of waterstress on crop growth[J]. Agricultural Reseach in the Arid Areas,2002,20(4):6-10.
[12]于文颖,纪瑞鹏,冯锐,等.不同程度干旱胁迫及复水对春玉米(丹玉39)茎流动态的影响[J].干旱地区农业研究,2016,34(02):163-170.
[13]李泽,谭晓风,卢锟,等.干旱胁迫对两种油桐幼苗生长、气体交换及叶绿素荧光参数的影响[J].生态学报,2017,37(05):1515-1524.
[14]卞凤娥,孙永江,牛彦杰,等.高温胁迫下根施褪黑素对葡萄叶片叶绿素荧光特性的影响[J].植物生理学报,2017,53(02):257-263.
[15]张强,杨平,张边江.叶绿素荧光技术在彩叶植物引种评价中的应用[J].分子植物育种,2017,15(03):1114-1120.
[16]刘世秋,张振文,惠竹梅,等.干旱胁迫对酿酒葡萄赤霞珠光合特性的影响[J].干旱地区农业研究,2008,(05):169-172.
[17]王振兴,陈丽,艾军,等.不同干旱胁迫对山葡萄的光合作用和光系统II活性的影响[J].植物生理学报,2014,50(08):1171-1176.
[18]胡宏远,王振平.干旱胁迫对赤霞珠葡萄叶片水分及叶绿素荧光参数的影响[J].干旱区资源与环境,2017,31(04):124-130.
[19] Burman U,Garg B.K.,Kathju S. Water relations,Photosynthesis andnitrogen metabolism of Indian mustard grown under salt and waterstress[J]. Journal of Plant Biology,2003,30(1):55-60.
[20] Sharma P N,Tripathi A,Bisht S S. Zinc requirement for stomatal o-pening in cauliflower[J]. Plant Physiology,1995,107:751-756.
[21]牛铁泉,田给林,薛仿正,等.半根及半根交替水分胁迫对苹果幼苗光合作用的影响[J].中国农业科学,2007,40(07):1463-1468.
[22]张继义,赵哈林.短期极端干旱事件干扰下退化沙质草地群落抵抗力稳定性的测度与比较[J].生态学报,2010,30(20):5456-5465.
[23]李金航,齐秀慧,徐程扬,等.黄栌幼苗叶片气体交换对干旱胁迫的短期响应[J].林业科学,2015,51(01):29-41.
[24] Medrano H,Escalona J M,Bota J,et al. Regulation of photosynthesisof C3plants in response to progressive drought:stomatal conductanceas a reference parameter[J]. Annals of botany,2002,89(7):895-905.
[25]杨文权,顾沐宇,寇建村,等.干旱及复水对小冠花光合及叶绿素荧光参数的影响[J].草地学报,2013,21(06):1130-1135.
[26]蒙祖庆,宋丰萍,刘振兴,等.干旱及复水对油菜苗期光合及叶绿素荧光特性的影响[J].中国油料作物学报,2012,34(01):40-47.
[27]袁军伟,李敏敏,刘长江,等.盐胁迫下马瑟兰/101-14嫁接苗和自根苗光合生理及叶绿素荧光参数的差异[J].西北农业学报,2016,25(05):758-762.
[28] Parida A K,das A B. Salt tolerance and salinity effects on plants:areview[J]. Ecotoxicology and Environmental Safety,2005,60(3):324-349.
[29]吴甘霖,段仁燕,王志高,等.干旱和复水对草莓叶片叶绿素荧光特性的影响[J].生态学报,2010,30(14):3941-3946.
[30] Bota J,Flexas J,Medrano H. Is photosynthesis limited by decreasedRubisco activity and Ru BP content unprogressive water stress[J].New Phytologist,2004,162(3):671-681.
[31] Schreiber U,Gademann R,Ralph P J. Assessment of photosyntheticperformance of prochloron in Lissoclinum patella in hospite by chloro-phyll fluorescence measurements[J]. Plant Cell and Physiology,1997,38(8):945-951.
[32] Massacci A,Nabiv S M,Pietrosanti L. Response of photosynthesis ap-paratus of cotton to the onset of drought stress under field conditionsby gas change analysis and chlorophyll fluorescence imaging[J].Plant Physiology and Biochemistry,2008,46(2):189-195.
[33] Santos M G,Ribeiro R V,Machado E C. Effects of drought stress onphotosynthetic parameters and leaf water potential of five commonbean genotypes under water deficit[J]. Biologia Plantarum,2009,53(2):229-236.
[34]赵丽英,邓西平,山仑.渗透胁迫对小麦幼苗叶绿素荧光参数的影响[J].应用生态学报,2005,16(07):1261-1264.
[35]厉广辉,万勇善,刘风珍,等.苗期干旱及复水条件下不同花生品种的光合特性[J].植物生态学报,2014,38(07):729-739.