微藻生物质生产燃料乙醇技术进展
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摘要
燃料乙醇是燃烧清洁的高辛烷值燃料,是可再生能源,也是优良的燃油改善剂。微藻是一种高光合效率、高生物量产值的生物质资源,部分微藻经光合作用可在胞内积累大量淀粉和纤维素,是制备燃料乙醇的良好原料。利用微藻生物质生产燃料乙醇,对于缓解石油资源日益紧缺的现状及解决一系列由温室气体引起的环境问题具有乐观的应用前景。介绍了微藻作为生物质供应的特性,综述了国内外对于微藻生产燃料乙醇的技术进展、现存在问题及未来的发展前景。
Bioethanol is a clean-burning high-octane fuel, a renewable energy, and an outstanding fuel additive. As a biomass resource with high photosynthetic efficiency and productivity, some microalgae can accumulate starch and cellulose within cells, which can be regarded as a capable feedstock for bioethanol production. Bioethanol production by using microalgal biomass is a promising future of assuaging the increasing lack of oil resources and solving the environmental problems caused by greenhouse gases. In this paper, the characteristic of microalgae as a biomass resource was introduced, the technical progress of global bioethanol production by using microalgal biomass was discussed as well as the present problems and the future prospects.
Bioethanol is a clean-burning high-octane fuel, a renewable energy, and an outstanding fuel additive. As a biomass resource with high photosynthetic efficiency and productivity, some microalgae can accumulate starch and cellulose within cells, which can be regarded as a capable feedstock for bioethanol production. Bioethanol production by using microalgal biomass is a promising future of assuaging the increasing lack of oil resources and solving the environmental problems caused by greenhouse gases. In this paper, the characteristic of microalgae as a biomass resource was introduced, the technical progress of global bioethanol production by using microalgal biomass was discussed as well as the present problems and the future prospects.
引文
[1] Waltz E. Biotech's green gold?[J].Nat Biotechnol, 2009, 27(1):15-18.
[2] CHISTI Y. Biodiesel from microalgae beats bioethanol[J]. Trends in Biotechnology, 2008, 26:126-131.
[3] WIJFFELS R H, BARBOSA M J. An outlook on microalgal biofuels[J]Science, 2010, 329(5993):796-799.
[4] LEE R E. Phycology[M]. New York:Cambridge University press, 1980.
[5] Smith V H, Sturm B S M, Denoyelles F J, et al. The ecology of algal biodiesel production[J]. Trends in Ecology&Evolution, 2010,25(5):301-309.
[6] Costa J A, De Morais M G. The role of biochemical engineering in the production of biofuels from microalgae[J]. Bioresour Technol,2011,102(1):2-9.
[7] POSTEN C, GEORG S. Microalgae and terrestrial biomass as source for fuels-A process view[J]. Journal of Biotechnology, 2009.
[8] Li Y, Horsman M, Nan Wu, et al. Biofuels from Microalgae[J].Biotechnology Progress, 2008, 24(4):815-820.
[9] Daroch M, Geng S, Wang G. Recent advances in production from algal feed stocks[J]. Applied Energy 2013, 102:1371-1381.
[10] SINGH A, NIGAM P S, MURPHY J D. Mechanism and challenges in commercialization of algal biofuels[J]. Bioresourch Technology, 2011,102:26-34.
[11]闫海，张宾，王素琴，等.小球藻异养培养的研究进展[J].现代化工，2007 27(4):18-21.Yan Hai, Zhang Bin, Wang Suqin, et a1.Advances in the heterotrophic culture of chlorella sp[J]. Modern Chemical Industry, 2007, 27(4):18-21.(in Chinese with English abstract.)
[12]张展，刘建国.微藻高密度培养中的生长指标和适应机制[J].海洋水产研究，2003, 24(4):36-43.Zhang Zhan, Liu Jianguo. Applications of growth parametersand adaptive mechanisms of microalgae cultured at high cell density[J]. Marine Fisheries Research, 2003, 24(4):36-43.(in Chinese with English abstract)
[13] SINGH A, NIGAM P S, MURPHY J D. Mechanism and challenges in commercialization of algal biofuels[J]. Bioresourch Technology, 2011,102:26-34.
[14] BRENNAN L, OWENDE P. Biofuels from microalgae-A review of technology for production, process, and extractions of biofuels and co-products[J]. Renewable and Sustainable Energy Review, 2010,14:557-577.
[15] HARUN R, SINGH M, FORDE G M, et al. Bioprocess engineering of microalgae to produce a variety of consumer products[J]. Renewable and Sustainable Energy Review, 2009.
[16] Yusuf Chisti. Biodiesel from microalgae[J]. Biotechnology Advances,2007, 25(3):294-306.
[17] Brennan L, Owende P. Biofuels from microalgae a review of technologies for production, processing, and extractions of biofuels and co-products[J]. Renewable and Sustainable Energy Reviews, 2010,14(2):557-577.
[18] Ugwu C U, Aoyagi H, Uchiyama H. Photo bioreactors for mass cultivation of algae[J]. Bioresource Technology, 2008, 99(10):4021-4028.
[19] MATSUMOTO M, YOKOUCHI H, SUZUKI N, et al.Sacchari-fication of marine microalgae using marine bacteria for ethanol production[J].Appl. Biochem. Biotechnol, 2003,105-108:247-254.
[20] CHUJI T, MASAHIRO O, IKUNOSUKE T. Method of obtaining ethanol by the utilization of unicellular green algae:CA, 195-2,CA1073379A1[P]. 1976-01-06.
[21] Shin M K, Kim G S, Kim Y J, et al. Method of producing biofuel using algae:Korea, C12P 7/06, WO 2008/105618 A1[P].2008-04-09.
[22] CHOI S P, NGUYEN M T, SIM A J. Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production[J].Bioresource Technology, 2011
[23] NGUYEN, THU M, CHOI S P, et al. Hydrothermal acid pretreatment of chlamydomonas reinhardtii biomass for ethanol production[J].Journal of microbiology and biotechnology, 2009, 19(2):161-166.
[24] Okuda K, Oka K, Onda A, et al. Hvdrothermal fractional pretreatment of sea algae and its enhanced enzymatic hydrolysis[J]. Journal of Chemical Technology&Biotechnology, 2008,83(6):836-841.
[25] Yu Y, I.ou X, Wu H W. Some recent advances in hydrolysis of biomass in hot-compressed, water and its comparisons with other hvdrolysis methods[J]. Energy&Fuels, 2008,22(1):46-60.
[26] Itoh, W ada M, Honda Y, et al. Biooiganosolve Pretreatments for Simultaneous Saccharification and Fermentation of Beech Wood by Ethanolysis and White Rot Fungi[J].J Biotechnol,2003, 103(3):273-280.
[27] Anderson W F, Peteison J, Akin D E,et al. Enzyme Pretreatment of Grass Lignocellulose for Potential High-Value Co-Products and an Improved Fementable Substrate[J]. Appl Biochem Biotechnol, 2005,121(1-3):303-310.
[28]王江龙，孜力汗，自凤.絮凝酵母SPSC01酒精连续发酵幵联产酵母工艺过[J].化工学报，2004,55(6):1024-1027
[29] Fan Zhiliang, Shao Xiongjun, Lynd L R. Lower Cellulose Requirements for Biomass Cellulose Hydrolysis and Fermentation:US Pat App l, US 20060014260[P]. 2006.
[30] UEDA R, HIRAYAMA S, SAGATA K, et al. Process for the production of ethanol from microalgae:US Patent, 5,578,472[P].1996-11-26.
[31] OHTA S, MIYAMOTO K, MIURA Y. Hydrogen evolution as a consumption mode of reducing equivalents in green algal fermentation[J]. Plant Physiol, 1987, 83:1022-1026.
[32] UEDA R, YOHEI H, SHIN S, et al. Process for the production of ethanol from microalgae[P]. US, 5578472, 1996-11-26.
[33] HIRANO A, UEDA R, HIRAYAMA S, et al. CO2 fixation and ethanol production with microalgal photosynthesis and intracellu-lar anaerobic fermentation[J].Energy, 1997,22:137-42.
[34] UENO Y, KURANO N, MIYACHI S. Ethanol production by dark fermentation in the marine green alga, Chlorococcum littorale[J].Journal of fermentation and bioengineering, 1998, 86(1):38-43.
[35] MOLL, BENJAMIN A. Methods and compositions for producing metabolic products for algae[P]. CA, 1991-07-12.
[36] DENG M, COLEMAN J. Ethanol synthesis by genetic engineering in cyanobacteria[J]. Appl. Environ. Microbiol., 1999,65:428-523.
[37] WOODS, PAUL R, ROBERT J, et al. Genetically modified cyanobacteria for the production ethanol, the constructs and method thereof:US, 6699696[P]. 2004-03-02.
[38] FU P C, PATRICK, JASON D. Methods and compositions for ethanol producing cyanobacteria:US, C12N 15/11[P]. 2007-07-26.
[39] LEE J W. Designer organisms for photosynthetic production of ethanol from carbon dioxide and water:US, US 2008/0176304 A1[P].2008-07-24.
[40] SHERIDAN C. Making green[J].Nat. Biotechnol.,2009,27:1074-1076.
[41]黄英明,王伟良,李元广,等.微藻能源技术开发和产业化的发展思路与策略[J].生物工程学报, 2010, 26(7):907-913.
[2] CHISTI Y. Biodiesel from microalgae beats bioethanol[J]. Trends in Biotechnology, 2008, 26:126-131.
[3] WIJFFELS R H, BARBOSA M J. An outlook on microalgal biofuels[J]Science, 2010, 329(5993):796-799.
[4] LEE R E. Phycology[M]. New York:Cambridge University press, 1980.
[5] Smith V H, Sturm B S M, Denoyelles F J, et al. The ecology of algal biodiesel production[J]. Trends in Ecology&Evolution, 2010,25(5):301-309.
[6] Costa J A, De Morais M G. The role of biochemical engineering in the production of biofuels from microalgae[J]. Bioresour Technol,2011,102(1):2-9.
[7] POSTEN C, GEORG S. Microalgae and terrestrial biomass as source for fuels-A process view[J]. Journal of Biotechnology, 2009.
[8] Li Y, Horsman M, Nan Wu, et al. Biofuels from Microalgae[J].Biotechnology Progress, 2008, 24(4):815-820.
[9] Daroch M, Geng S, Wang G. Recent advances in production from algal feed stocks[J]. Applied Energy 2013, 102:1371-1381.
[10] SINGH A, NIGAM P S, MURPHY J D. Mechanism and challenges in commercialization of algal biofuels[J]. Bioresourch Technology, 2011,102:26-34.
[11]闫海，张宾，王素琴，等.小球藻异养培养的研究进展[J].现代化工，2007 27(4):18-21.Yan Hai, Zhang Bin, Wang Suqin, et a1.Advances in the heterotrophic culture of chlorella sp[J]. Modern Chemical Industry, 2007, 27(4):18-21.(in Chinese with English abstract.)
[12]张展，刘建国.微藻高密度培养中的生长指标和适应机制[J].海洋水产研究，2003, 24(4):36-43.Zhang Zhan, Liu Jianguo. Applications of growth parametersand adaptive mechanisms of microalgae cultured at high cell density[J]. Marine Fisheries Research, 2003, 24(4):36-43.(in Chinese with English abstract)
[13] SINGH A, NIGAM P S, MURPHY J D. Mechanism and challenges in commercialization of algal biofuels[J]. Bioresourch Technology, 2011,102:26-34.
[14] BRENNAN L, OWENDE P. Biofuels from microalgae-A review of technology for production, process, and extractions of biofuels and co-products[J]. Renewable and Sustainable Energy Review, 2010,14:557-577.
[15] HARUN R, SINGH M, FORDE G M, et al. Bioprocess engineering of microalgae to produce a variety of consumer products[J]. Renewable and Sustainable Energy Review, 2009.
[16] Yusuf Chisti. Biodiesel from microalgae[J]. Biotechnology Advances,2007, 25(3):294-306.
[17] Brennan L, Owende P. Biofuels from microalgae a review of technologies for production, processing, and extractions of biofuels and co-products[J]. Renewable and Sustainable Energy Reviews, 2010,14(2):557-577.
[18] Ugwu C U, Aoyagi H, Uchiyama H. Photo bioreactors for mass cultivation of algae[J]. Bioresource Technology, 2008, 99(10):4021-4028.
[19] MATSUMOTO M, YOKOUCHI H, SUZUKI N, et al.Sacchari-fication of marine microalgae using marine bacteria for ethanol production[J].Appl. Biochem. Biotechnol, 2003,105-108:247-254.
[20] CHUJI T, MASAHIRO O, IKUNOSUKE T. Method of obtaining ethanol by the utilization of unicellular green algae:CA, 195-2,CA1073379A1[P]. 1976-01-06.
[21] Shin M K, Kim G S, Kim Y J, et al. Method of producing biofuel using algae:Korea, C12P 7/06, WO 2008/105618 A1[P].2008-04-09.
[22] CHOI S P, NGUYEN M T, SIM A J. Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production[J].Bioresource Technology, 2011
[23] NGUYEN, THU M, CHOI S P, et al. Hydrothermal acid pretreatment of chlamydomonas reinhardtii biomass for ethanol production[J].Journal of microbiology and biotechnology, 2009, 19(2):161-166.
[24] Okuda K, Oka K, Onda A, et al. Hvdrothermal fractional pretreatment of sea algae and its enhanced enzymatic hydrolysis[J]. Journal of Chemical Technology&Biotechnology, 2008,83(6):836-841.
[25] Yu Y, I.ou X, Wu H W. Some recent advances in hydrolysis of biomass in hot-compressed, water and its comparisons with other hvdrolysis methods[J]. Energy&Fuels, 2008,22(1):46-60.
[26] Itoh, W ada M, Honda Y, et al. Biooiganosolve Pretreatments for Simultaneous Saccharification and Fermentation of Beech Wood by Ethanolysis and White Rot Fungi[J].J Biotechnol,2003, 103(3):273-280.
[27] Anderson W F, Peteison J, Akin D E,et al. Enzyme Pretreatment of Grass Lignocellulose for Potential High-Value Co-Products and an Improved Fementable Substrate[J]. Appl Biochem Biotechnol, 2005,121(1-3):303-310.
[28]王江龙，孜力汗，自凤.絮凝酵母SPSC01酒精连续发酵幵联产酵母工艺过[J].化工学报，2004,55(6):1024-1027
[29] Fan Zhiliang, Shao Xiongjun, Lynd L R. Lower Cellulose Requirements for Biomass Cellulose Hydrolysis and Fermentation:US Pat App l, US 20060014260[P]. 2006.
[30] UEDA R, HIRAYAMA S, SAGATA K, et al. Process for the production of ethanol from microalgae:US Patent, 5,578,472[P].1996-11-26.
[31] OHTA S, MIYAMOTO K, MIURA Y. Hydrogen evolution as a consumption mode of reducing equivalents in green algal fermentation[J]. Plant Physiol, 1987, 83:1022-1026.
[32] UEDA R, YOHEI H, SHIN S, et al. Process for the production of ethanol from microalgae[P]. US, 5578472, 1996-11-26.
[33] HIRANO A, UEDA R, HIRAYAMA S, et al. CO2 fixation and ethanol production with microalgal photosynthesis and intracellu-lar anaerobic fermentation[J].Energy, 1997,22:137-42.
[34] UENO Y, KURANO N, MIYACHI S. Ethanol production by dark fermentation in the marine green alga, Chlorococcum littorale[J].Journal of fermentation and bioengineering, 1998, 86(1):38-43.
[35] MOLL, BENJAMIN A. Methods and compositions for producing metabolic products for algae[P]. CA, 1991-07-12.
[36] DENG M, COLEMAN J. Ethanol synthesis by genetic engineering in cyanobacteria[J]. Appl. Environ. Microbiol., 1999,65:428-523.
[37] WOODS, PAUL R, ROBERT J, et al. Genetically modified cyanobacteria for the production ethanol, the constructs and method thereof:US, 6699696[P]. 2004-03-02.
[38] FU P C, PATRICK, JASON D. Methods and compositions for ethanol producing cyanobacteria:US, C12N 15/11[P]. 2007-07-26.
[39] LEE J W. Designer organisms for photosynthetic production of ethanol from carbon dioxide and water:US, US 2008/0176304 A1[P].2008-07-24.
[40] SHERIDAN C. Making green[J].Nat. Biotechnol.,2009,27:1074-1076.
[41]黄英明,王伟良,李元广,等.微藻能源技术开发和产业化的发展思路与策略[J].生物工程学报, 2010, 26(7):907-913.